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Techniques In Somatic Cell Genetics
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Book Synopsis Techniques in Somatic Cell Genetics by : Jerry W. Shay
Download or read book Techniques in Somatic Cell Genetics written by Jerry W. Shay and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu lating following the characterization of cytoplasmic mutations. The produc tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.
Book Synopsis Genetic Engineering of Plants by : National Research Council
Download or read book Genetic Engineering of Plants written by National Research Council and published by National Academies Press. This book was released on 1984-02-01 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."
Book Synopsis Somatic Gene Therapy by : P.L. Chang
Download or read book Somatic Gene Therapy written by P.L. Chang and published by CRC Press. This book was released on 2018-01-17 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: As human gene therapy becomes a clinical reality, a new era in medicine dawns. Novel and innovative developments in molecular genetics now provide opportunities to treat the genetic bases of diseases often untreatable before. Somatic Gene Therapy documents these historical clinical trials, reviews current advances in the field, evaluates the use of the many different cell types and organs amenable to gene transfer, and examines the prospects of various exciting strategies for gene therapy.
Author :National Academies of Sciences, Engineering, and Medicine Publisher :National Academies Press ISBN 13 :0309452880 Total Pages :329 pages Book Rating :4.3/5 (94 download)
Book Synopsis Human Genome Editing by : National Academies of Sciences, Engineering, and Medicine
Download or read book Human Genome Editing written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2017-08-13 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genome editing is a powerful new tool for making precise alterations to an organism's genetic material. Recent scientific advances have made genome editing more efficient, precise, and flexible than ever before. These advances have spurred an explosion of interest from around the globe in the possible ways in which genome editing can improve human health. The speed at which these technologies are being developed and applied has led many policymakers and stakeholders to express concern about whether appropriate systems are in place to govern these technologies and how and when the public should be engaged in these decisions. Human Genome Editing considers important questions about the human application of genome editing including: balancing potential benefits with unintended risks, governing the use of genome editing, incorporating societal values into clinical applications and policy decisions, and respecting the inevitable differences across nations and cultures that will shape how and whether to use these new technologies. This report proposes criteria for heritable germline editing, provides conclusions on the crucial need for public education and engagement, and presents 7 general principles for the governance of human genome editing.
Book Synopsis Safety of Genetically Engineered Foods by : National Research Council
Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Book Synopsis Advances in Gene Technology: Molecular Genetics of Plants and Animals by : Kathleen Downey
Download or read book Advances in Gene Technology: Molecular Genetics of Plants and Animals written by Kathleen Downey and published by Elsevier. This book was released on 2013-10-22 with total page 636 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Gene Technology: Molecular Genetics of Plants and Animals contains the proceedings of the Miami Winter Symposium held in January 1983 in Miami, Florida. The papers explore advances in the molecular genetics of plants and animals and cover a wide range of topics such as genetic manipulation of plants; plant cell cultures, regeneration, and somatic cell fusion; and nitrogen fixation. Practical applications of gene technology with plants are also discussed. Comprised of 84 chapters, this volume begins with an overview of how plants manufacture from carbon dioxide and water all of their substances, paying particular attention to the path of carbon in photosynthesis. The organization of the plant genome is then considered, along with techniques for cell culture, regeneration, and somatic cell fusion; vector systems; and nitrogen fixation. Some chapters focus on gene transfer by protoplast fusion; somatic cell genetic systems in corn; regulation of transcription of the nitrogen fixation operons; and leghemoglobin and nodulin genes of soybean. The final section is devoted to practical applications of gene technology to plants and to technology frontiers in animal biology, in particular embryonic development and vaccines and diagnostic methods for animal diseases. This book should be of value to molecular geneticists.
Book Synopsis Plant Improvement and Somatic Cell Genetics by : Indra Asil
Download or read book Plant Improvement and Somatic Cell Genetics written by Indra Asil and published by Elsevier. This book was released on 2012-12-02 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Improvement and Somatic Cell Genetics includes all but one of the papers presented at two symposia held during the XIII International Botanical Congress in Sydney, Australia, on August 21-28, 1981. ""Frontiers in Plant Breeding"" and ""Cell Culture and Somatic Cell Genetics in Plant Biology"" highlight the ways in which plant breeding techniques can improve crops. The book explores the potentials as well as the limitations of plant breeding, and cellular and molecular techniques in plant improvement. Comprised of 14 chapters, this volume begins with an overview of the potential applications of exotic germplasm for tomato and cereal crop improvement. It continues with a discussion of multiline breeding, breeding of crop plants that can tolerate soil stresses, combining genomes by means of conventional methods, use of embryo culture in interspecific hybridization, use of haploids in plant improvement, and somaclonal variation and somatic hybridization as new techniques for plant improvement. The reader is also introduced to plant cell culture, as well as somatic cell genetics of cereals and grasses, somatic cell fusion for inducing cytoplasmic exchange, uses of cell culture mutants, genetic transformation of plant cells by experimental procedures in the context of plant genetic engineering, and use of molecular biology techniques for recognition and modification of crop plant genotypes. This book will be a useful resource for scientists and plant breeders interested in applying somatic cell genetics for crop improvement.
Book Synopsis Heritable Human Genome Editing by : The Royal Society
Download or read book Heritable Human Genome Editing written by The Royal Society and published by National Academies Press. This book was released on 2021-01-16 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight.
Book Synopsis Somatic Cell Genetics by : C. Thomas Caskey
Download or read book Somatic Cell Genetics written by C. Thomas Caskey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mapping and Sequencing the Human Genome by : National Research Council
Download or read book Mapping and Sequencing the Human Genome written by National Research Council and published by National Academies Press. This book was released on 1988-01-01 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.
Book Synopsis Distant Hybridization of Crop Plants by : G. Kalloo
Download or read book Distant Hybridization of Crop Plants written by G. Kalloo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wild taxa are invaluable sources of resistance to diseases, insects/ pests, nematodes, temperature extremes, salinity and alkalinity stresses, and also of nutritional quality; adaptation; genetic diversity and new species. Utilization of wild relatives of a crop depends largely upon its crossability relations with cultivated varieties. Sev eral wild species are not crossable with the commercial cultivars due to various isolation barriers. Furthermore, in a few cases, hybridiza tion is possible only in one direction and reciprocal crosses are not successful, thus depriving the utilization of desired cytoplasm of many species. However, techniques have been developed to over come many barriers and hybrid plants are produced. New crop species have been developed by overcoming the F 1 sterility and producing amphidiploids and such crops are commercially being grown in the field. The segregation pattern ofF 1 hybrids produced by distant hybridization in segregating generations are different from the intervarietal hybrids. In former cases, generally, unidirectional segregation takes place in early generations and accordingly, selec tion procedures are adopted. In most of the cases, backcross or modified backcross methods have been followed to utilize wild species, and thus numerous types of resistance and other economical attributes have been transferred in the recurrent parents. Protoplast fusion has been amply demonstrated in a number of cases where sexual hybridization was not possible and, as a result, hybrids have been produced.
Book Synopsis Nuclear Transfer Protocols by : Paul J. Verma
Download or read book Nuclear Transfer Protocols written by Paul J. Verma and published by Springer Science & Business Media. This book was released on 2006-07-18 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
Book Synopsis Scientific and Medical Aspects of Human Reproductive Cloning by : National Research Council
Download or read book Scientific and Medical Aspects of Human Reproductive Cloning written by National Research Council and published by National Academies Press. This book was released on 2002-06-17 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.
Download or read book Cell Hybrids written by Nils R. Ringertz and published by Academic Press. This book was released on 2014-06-28 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Hybrids summarizes the methodology of cell fusion-the fusion of human, animal, and plant cells of different origins to produce cell hybrids-and surveys the main applications and current findings of the hybridization technique. The book opens with a chapter on the history cell hybridization. This is followed by separate chapters on spontaneous cell fusion, virus-induced cell fusion, the cell fusion mechanism, regulation of DNA synthesis and mitosis in heterokaryons and homokaryons, and regulatory events which occur when two cells with different nuclear activity and/or phenotype are fused with each other. Subsequent chapters deal with methods used in preparing various cell fragments and some of their properties and uses in fusion experiments; isolation of growing hybrid cells; chromosome patterns and phenotypic expression in hybrid cells; cell organelles in hybrid cells; analysis of malignancy by cell hybridization. The final chapters discuss the use of somatic cell hybridization to analyze the interaction between a number of viruses and their host cells; and the use of plant cell hybrids.
Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard
Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Somatic Cell Hybrids written by Abbott and published by Oxford University Press, USA. This book was released on 1995-03 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describing the background to a technique widely used in human genetics, this study analyzes the uses of a particular type of cell culture. These uses include the mapping of human genes, a key aspect of the human genome project.
