Author : Mark C. Leake
Publisher : Springer Nature
ISBN 13 : 1071622218
Total Pages : 332 pages
Book Rating : 4.0/5 (716 download)
Book Synopsis Chromosome Architecture by : Mark C. Leake
Download or read book Chromosome Architecture written by Mark C. Leake and published by Springer Nature. This book was released on 2022-05-30 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.