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Studies On Immobilization Of Alcohol Dehydrogenase And Cells Of Saccharomyces Cerevisiae For Improving Ethanol Production
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Book Synopsis Studies on Immobilization of Alcohol Dehydrogenase and Cells of Saccharomyces Cerevisiae for Improving Ethanol Production by : Sarabjot Kaur
Download or read book Studies on Immobilization of Alcohol Dehydrogenase and Cells of Saccharomyces Cerevisiae for Improving Ethanol Production written by Sarabjot Kaur and published by . This book was released on 2000 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Ethanol Production From Saccharomyces Cerevisiae by : Sarabjot Kaur
Download or read book Ethanol Production From Saccharomyces Cerevisiae written by Sarabjot Kaur and published by LAP Lambert Academic Publishing. This book was released on 2014-09-10 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ethanol is an important industrial solvent and a chemical feedstock being used for the synthesis of pharmaceuticals, detergents, adhesives, plastics, plasticizers and host for other chemicals. To reduce the cost of ethanol production, significant improvements in ethanol production technology are needed. The potential advantages of immobilized whole cell system have been recognized in the fermentation of a wide variety of carbohydrates. Immobilized cell technology has many advantages over conventional systems .This study is aimed at cell immobilization process, isolation, purification and immobilization of alcohol dehydrogenase(ADH) on different matrices from cells of S.Cerevisiae for enhancing ethanol production. The cells of Saccharomyces Cerevisiae were immobilized on different matrices viz polacrylamide, k-carrageenan, agar and calcium-alginate .Ethanol Production was improved by immobilized Alcohol Dehydrogenase and further enhanced when cell free extract was co-immobilized with isolated ADH
Book Synopsis A Study of Fermentation Behavior and Alcohol Dehydrogenase Activity in the Yeast Saccharomyces Cerevisiae by : Harry David Hansen
Download or read book A Study of Fermentation Behavior and Alcohol Dehydrogenase Activity in the Yeast Saccharomyces Cerevisiae written by Harry David Hansen and published by . This book was released on 1986 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Production of Ethanol by Immobilized Saccharomyces Cerevisiae by : Noordiana Ariffin
Download or read book Production of Ethanol by Immobilized Saccharomyces Cerevisiae written by Noordiana Ariffin and published by . This book was released on 2014 with total page 58 pages. Available in PDF, EPUB and Kindle. Book excerpt: This research was conducted on the fermentation of ethanol by Saccharomyces cerevisiae using immobilization technique. S. cerevisiae which is also known as Baker's yeast is a single-celled eukaryote that is frequently used in scientific research. It is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated and very easy to maintain in the laboratory. Besides, immobilization has already been approved as useful method in maintaining high cell concentration within the reactor, increases the cell's ability to tolerate high ethanol concentration in the medium, allowing higher conversion yield and increased volumetric productivities. Therefore, the purpose of this study is to immobilize the yeast using various kind of supports (e.g: muslin cloth, membrane and sugarcane stalk) in batch processes to determine the best support for ethanol production. In this method, immobilized fermentation was carried out through shake flask seeding technique while the duration of fermentation process was fixed at 10 hours with sampling made at every 2 hours intervals. The germination of the stock and seed culture preparations were conducted followed by the immobilization technique in order to produce ethanol. Samples taken were analyzed for ethanol and glucose concentration, colony forming unit (CFU), biomass concentration, optical density and viewing of S. cerevisiae attachment on immobilized supports by Scanning Electron Microscope (SEM). From the result obtained, it showed that immobilized cloth was capable to increase cell optical density up to 1.602 after 10 hours of fermentation. Moreover, analysis performed by using biomass has revealed that immobilized cloth at 10 hours of fermentation gave the highest biomass concentration which is 39.64 gL-1 and colonies of cells forming at 2.57 x 108/mL of viable cells compared to other materials. Besides, its ethanol productivity was enhanced to 0.42 gL-1h-1, being over 9.6% higher than that observed in the batch culture (0.38 gL-1h-1). The consumption of glucose was improved by 6.9% (compared to batch operation), where nearly 67% of glucose conversion at 10 hours period of immobilized fermentation through muslin cloth. The increment might be associated with the altered metabolical functions in the immobilized cells. This alteration is attributed to the reduction of the diffusion path of the growth nutrient that enhanced the availability and promoted the growth of yeast, thus improving the catalytic conversion of glucose to ethanol. Therefore, the supported muslin cloth was proved to increase the number of cells and glucose inhibition is able to reduce with the utilization of immobilized S. cerevisiae, thus increasing ethanol production.
Book Synopsis Optimization Studies for Ethanol Production Using Immobilized Saccharomyces Cerevisiae Cells by : Anita Sheoran
Download or read book Optimization Studies for Ethanol Production Using Immobilized Saccharomyces Cerevisiae Cells written by Anita Sheoran and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fungal Metabolites by : Jean-Michel Mérillon
Download or read book Fungal Metabolites written by Jean-Michel Mérillon and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Yeast written by Antonio Morata and published by BoD – Books on Demand. This book was released on 2017-11-08 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast - Industrial Applications is a book that covers applications and utilities of yeasts in food, chemical, energy, and environmental industries collected in 12 chapters. The use of yeasts in the production of metabolites, enzymatic applications, fermented foods, microorganism controls, bioethanol production, and bioremediation of contaminated environments is covered showing results, methodologies, and processes and describing the specific role of yeasts in them. The traditional yeast Saccharomyces cerevisiae is complemented in many applications with the use of less known non-Saccharomyces yeasts that now are being used extensively in industry. This book compiles the experience and know-how of researchers and professors from international universities and research centers.
Book Synopsis Assessment of Immobilized Cell Systems for Ethanol Production Using Z. Mobilis and S. Cerevisiae by : Gerald Ernest Rowe
Download or read book Assessment of Immobilized Cell Systems for Ethanol Production Using Z. Mobilis and S. Cerevisiae written by Gerald Ernest Rowe and published by . This book was released on 1981 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Immobilization of Enzymes and Cells by : Jose M. Guisan
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2016-08-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Book Synopsis Studies on Alcohol Dehydrogenase During Growth and Sporulation of the Yeast Saccharomyces Cerevisiae by : Y. E. Eltayeb
Download or read book Studies on Alcohol Dehydrogenase During Growth and Sporulation of the Yeast Saccharomyces Cerevisiae written by Y. E. Eltayeb and published by . This book was released on 1978 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Enhancing the Ethanol Pathway in Escherichia Coli RM10 for Improved Ethanol Production from Xylose by : Ryan Allan Manow
Download or read book Enhancing the Ethanol Pathway in Escherichia Coli RM10 for Improved Ethanol Production from Xylose written by Ryan Allan Manow and published by . This book was released on 2016 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to its capability to ferment biomass-derived pentose and hexose, Escherichia coli is considered a potential biocatalyst for production of bioethanol. Gene deletions and promoter replacements were previously employed to engineer a non-transgenic homoethanol pathway in E. coli [glucose (or 1.2 xylose) => (glycolysis) => 2 pyruvate + 2 NADH => (pyruvate dehydrogenase, aceEF-lpd operon) => 2 acetyl-CoA + 4 NADH => (alcohol dehydrogenase, adhE) => 2 acetaldehyde + 2 NADH => (alcohol dehydrogenase) => 2 ethanol]. The resulting strain, RM10, is able to produce ethanol from xylose and glucose, with a yield greater than 90% theoretical (one mole of glucose to 2 mole of ethanol). In this study, the transcription of five genes involved in the homoethanol pathway [ aceEF-lpd, adhE, and aldB (encoding for aldehyde dehydrogenase)] were overexpressed in RM10 for improved ethanol fermentation. Acetaldehyde is an intermediate of the homoethanol pathway. In theory, blocking the oxidation of acetaldehyde (catalyzed by aldehyde dehydrogenase) will prevent diverting the carbon skeleton to acetic acid, which should in turn improve ethanol production. Contrary to this hypothesis, however, blocking acetaldehyde oxidation (deletion of aldB) resulted in decreased ethanol production compared to that of the parent strain. Furthermore, overexpression of aldB increased ethanol production over that achieved by the parent strain. The improved ethanol production likely attributes to improved acetaldehyde tolerance because the aldB overexpression showed an improved acetaldehyde tolerance than the parent strain. The anaerobically expressed pyruvate dehydrogenase complex (PDH) is encoded by a three-gene operon (aceEF-lpd) and is essential to ethanol production by RM10. Previous studies suggested that PDH expression might be one of the limiting steps for ethanol fermentation. This hypothesis was tested by overexpression of the PDH via: 1) cloning aceE, aceF, lpd and the complete aceEF-lpd operon into a high copy plasmid; 2) integrating of a second copy of aceEF-lpd into chromosome. The results showed that: 1) overexpression of aceEF-lpd operon significantly improved cell growth and ethanol production; 2) chromosomal integration of a second copy of the aceEF-lpd operon did not show an improved transcription rate nor improved ethanol production; 3) overexpression of individual aceE , aceF, lpd genes decreased cell growth and ethanol fermentation. Alcohol dehydrogenase (adhE) is the final enzyme of the homoethanol pathway in RM10. Prior studies suggested that deletion of RNase G resulted in an increase of adhE mRNA, which in turn improved cell growth and ethanol fermentation. We assessed the impact overexpression of adhE on ethanol production by increasing the adhE copy number and by promoter replacement. The results showed that overexpression of adhE had a beneficial impact on cellular growth and ethanol production. Additionally, replacing the native adhE promoter with a pflBp6 promoter further enhanced ethanol fermentation. Nevertheless, chromosomal integration of a second copy of adhE with either pflBp6 promoter or native promoter did not achieve the improved adhE expression nor improved ethanol fermentation. In summary, the expression of aldB, aceEF-lpd , and adhE appears to be a rate-limiting factor during RM10 xylose fermentation. Successful overexpression of those genes resulted in improvements in cellular growth and/or ethanol production. Increasing chromosomal expression of genes involved in the homoethanol pathway should improve the fermentative capabilities of RM10 and move non-transgenic E. coli forward as an option for industrial bioethanol production.
Book Synopsis Handbook on Bioethanol by : Charles Wyman
Download or read book Handbook on Bioethanol written by Charles Wyman and published by Routledge. This book was released on 2018-05-02 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol is a versatile transportation fuel and fuel additive that offers excellent performance and reduced air pollution compared to conventional fuels. Its production and use adds little, if any, net release of carbon dioxide to the atmosphere, dramatically reducing the potential for global climate change. Through a sustained research program and an emerging economic competitiveness, the technology for bioethanol production is poised for immediate widespread commercial applications. Written by engineers and scientists providing a technical focus, this handbook provides the up-to-date information needed by managers, engineers, and scientists to evaluate the technology, market, and economics of this fuel, while examining the development of production required to support its commercial use.
Download or read book Biofuels written by Dwight Tomes and published by Springer Science & Business Media. This book was released on 2010-11-16 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive volume developed under the guidance of guest editors Prakash Lakshmanan and David Songstad features broad coverage of the topic of biofuels and its significance to the economy and to agriculture. These chapters were first published by In Vitro Cellular and Developmental Biology In Vitro Plant in 2009 and consists of 15 chapters from experts who are recognized both for their scientific accomplishments and global perspective in their assigned topics.
Book Synopsis Biochemical Engineering and Biotechnology by : Ghasem Najafpour
Download or read book Biochemical Engineering and Biotechnology written by Ghasem Najafpour and published by Elsevier. This book was released on 2015-02-24 with total page 669 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical Engineering and Biotechnology, 2nd Edition, outlines the principles of biochemical processes and explains their use in the manufacturing of every day products. The author uses a diirect approach that should be very useful for students in following the concepts and practical applications. This book is unique in having many solved problems, case studies, examples and demonstrations of detailed experiments, with simple design equations and required calculations. - Covers major concepts of biochemical engineering and biotechnology, including applications in bioprocesses, fermentation technologies, enzymatic processes, and membrane separations, amongst others - Accessible to chemical engineering students who need to both learn, and apply, biological knowledge in engineering principals - Includes solved problems, examples, and demonstrations of detailed experiments with simple design equations and all required calculations - Offers many graphs that present actual experimental data, figures, and tables, along with explanations
Book Synopsis Non-conventional Yeasts: from Basic Research to Application by : Andriy Sibirny
Download or read book Non-conventional Yeasts: from Basic Research to Application written by Andriy Sibirny and published by Springer. This book was released on 2019-08-12 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.
Book Synopsis Immobilized Microbial Cells by : Ichiro Chibata
Download or read book Immobilized Microbial Cells written by Ichiro Chibata and published by Elsevier. This book was released on 2014-06-28 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Download or read book Wine Fermentation written by Harald Claus and published by MDPI. This book was released on 2019-03-28 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wineries are facing new challenges due to actual market demands for the creation of products exhibiting more particular flavors. In addition, climate change has lead to the requirement for grape varieties with specific features, such as convenient maturation times, enhanced tolerance towards dryness, osmotic stress, and resistance against plant-pathogens. The next generation of yeast starter cultures should produce wines with an appealing sensory profile and less alcohol. This Special Issue comprises actual studies addressing some of the problems and solutions for the environmental, technical, and consumer challenges of wine making today: Development of sophisticated mass spectroscopic methods enable the identification of the major metabolite spectrum of grapes/wine and deliver detailed insights in terroir and yeast-specific traits;Knowledge of the origin and reactions of reductive sulphur compounds facilitates the avoidance of unpleasant wine odors;Innovative physical–chemical treatments support effective and sustainable color extraction from red grape varieties;Enological enzymes from yeasts used directly or in the form of starter cultures are promising tools to increase the juice yields, color intensity, and aroma of wine;Natural and artificial Saccharomyces hybrids as well as collections of adapted wild isolates from various ecological niches will extend winemakers repertoire, allowing individual fermentations;Exact process control of wine fermentations by convenient computer programs will guarantee consistently high product quality.
