Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Structural Investigation Of Membrane Proteins By Electron Microscopy
Download Structural Investigation Of Membrane Proteins By Electron Microscopy full books in PDF, epub, and Kindle. Read online Structural Investigation Of Membrane Proteins By Electron Microscopy ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Structural Investigation of Membrane Proteins by Electron Microscopy by : Katarzyna Beata Mościcka
Download or read book Structural Investigation of Membrane Proteins by Electron Microscopy written by Katarzyna Beata Mościcka and published by . This book was released on 2009 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structural Analysis of Membrane Proteins by Electron Microscopy by : Thomas Walz
Download or read book Structural Analysis of Membrane Proteins by Electron Microscopy written by Thomas Walz and published by . This book was released on 1996 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Structural Analysis of Membrane Proteins as Determined by High Resolution Electron Microscopy by : E. Loren Buhle
Download or read book The Structural Analysis of Membrane Proteins as Determined by High Resolution Electron Microscopy written by E. Loren Buhle and published by . This book was released on 1984 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Membrane Proteins Production for Structural Analysis by : Isabelle Mus-Veteau
Download or read book Membrane Proteins Production for Structural Analysis written by Isabelle Mus-Veteau and published by Springer. This book was released on 2014-06-20 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.
Book Synopsis Techniques for the Analysis of Membrane Proteins by : C. Ragan
Download or read book Techniques for the Analysis of Membrane Proteins written by C. Ragan and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: A preface should justify the existence of the book it precedes and this is invariably done in scientific texts by reference to the explosive growth of the field since the last such volume appeared. In molecular biology, most fields can be justifiably described as growing explosively, as should be the case for a young and vigorous science, but the study of membrane proteins stands out as one which has taken giant strides in the last few years. Ignorance of the structure and function of membrane proteins at the molecular level was certainly not due to lack of interest but rather was a result of lack of appropriate techniques. It has above all been the development of new experimental methods which has wrenched membrane biochemistry out of what Anthony Martonosi fetchingly called its 'romantic phase' (Le. lots of ideas and few facts), into an era when the determination of membrane protein structure and mechanism is a reasonable goal. Membrane proteins are generally classified as peripheral or integral. Peripheral proteins are relatively easily dissociated from membranes by mild treatments whence their study is essentially no different to that of soluble proteins. This book therefore concentrates on integral proteins which are strongly bound to the membrane by hydrophobic interactions with lipids. A crucial step in their study is of necessity the d~velopment of methods of solubilization and purification under non-denaturing conditions.
Book Synopsis Membrane Protein Complexes: Structure and Function by : J. Robin Harris
Download or read book Membrane Protein Complexes: Structure and Function written by J. Robin Harris and published by Springer. This book was released on 2018-02-20 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book contains a compilation of 14 advanced academic chapters dealing with the structure and function of membrane protein complexes. This rapidly advancing important field of study closely parallels those on soluble protein complexes, and viral protein and nucleoprotein complexes. Diverse topics are included in this book, ranging from membrane–bound enzymes to ion channels, proton pumps and photosystems. Data from X-ray crystallography, cryo-electron microscopy and other biophysical and biochemical techniques are presented throughout the book. There is extensive use of colour figures of protein structures. Throughout the book structure and function are closely correlated. The two editors, Egbert Boekema and J. Robin Harris, have worked on aspects of membrane and soluble proteins throughout their scientific careers and also have much publishing experience. The Subcellular Biochemistry series has expanded considerably in recent years, including several related volumes. The theme of protein complexes will be continued within several future volumes, thereby creating encyclopaedic coverage. The chapter topics within this book are particularly relevant to those involved in the biological and biomedical sciences. It is aimed at the advanced undergraduates, postgraduates and established researchers within this broad field. It is hoped that the book will be of interest and use to those involved with the study of cellular membranes and their associated proteins.
Book Synopsis Membrane Protein Structure Determination by : Jean-Jacques Lacapère
Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Methods in Molecular Biology. This book was released on 2010-08-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.
Book Synopsis Biophysical Analysis of Membrane Proteins by : Eva Pebay-Peyroula
Download or read book Biophysical Analysis of Membrane Proteins written by Eva Pebay-Peyroula and published by John Wiley & Sons. This book was released on 2008-06-25 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.
Book Synopsis Membrane Proteins in Aqueous Solutions by : Jean-Luc Popot
Download or read book Membrane Proteins in Aqueous Solutions written by Jean-Luc Popot and published by Springer. This book was released on 2018-06-08 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications. Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users. Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.
Book Synopsis Structural Analysis of Integral Membrane Proteins by Freeze-fracture Electron Microscopy by : Sepehr Eskandari
Download or read book Structural Analysis of Integral Membrane Proteins by Freeze-fracture Electron Microscopy written by Sepehr Eskandari and published by . This book was released on 1999 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structural Studies of Membrane Proteins Using Transmission Electron Microscopy by :
Download or read book Structural Studies of Membrane Proteins Using Transmission Electron Microscopy written by and published by . This book was released on 2015 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Membrane Protein Structure and Function Characterization by : Jean-Jacques Lacapere
Download or read book Membrane Protein Structure and Function Characterization written by Jean-Jacques Lacapere and published by Humana. This book was released on 2018-06-10 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Structural Analysis of Membrane Protein Biogenesis and Ribosome Stalling by Cryo-electron Microscopy by : Lukas Bischoff
Download or read book Structural Analysis of Membrane Protein Biogenesis and Ribosome Stalling by Cryo-electron Microscopy written by Lukas Bischoff and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structural Biology in Drug Discovery by : Jean-Paul Renaud
Download or read book Structural Biology in Drug Discovery written by Jean-Paul Renaud and published by John Wiley & Sons. This book was released on 2020-01-09 with total page 1367 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins
Book Synopsis The Next Generation in Membrane Protein Structure Determination by : Isabel Moraes
Download or read book The Next Generation in Membrane Protein Structure Determination written by Isabel Moraes and published by Springer. This book was released on 2018-09-19 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.
Book Synopsis Investigating the Structure of Macromolecular Complexes and Membrane Proteins Using Transmission Electron Microscopy by : Breanna Sachiko Vollmar
Download or read book Investigating the Structure of Macromolecular Complexes and Membrane Proteins Using Transmission Electron Microscopy written by Breanna Sachiko Vollmar and published by . This book was released on 2013 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transmission electron microscopy (TEM) has provided atomic resolution details regarding the biological processes that give rise to life. The work presented here focuses on the application of TEM for studying membrane proteins and macromolecular assemblies using electron crystallography and single particle electron cryomicroscopy (cryo-EM). Two membrane proteins, human aquaporin 3 (hAQP3) and galactose permease (GalP) from Escherichia coli, were studied using electron crystallography. hAQP3 was successfully expressed in Pichia pastoris and purified, but failed to reconstitute to form two-dimensional crystals. Two-dimensional crystals of GalP were optimized to form large, crystalline vesicles and sheets. A projection map of GalP calculated from a cryo-EM image showed that GalP forms trimers in the two-dimensional crystals. Single particle TEM studies focused on three macromolecular assemblies, alphaB-crystallin, a designed octahedral protein cage and a mutant HCV IRES-40S ribosome complex. Projection class averages of negatively stained alphaB-crystallin were combined with NMR and SAXS data to produce a pseudo-atomic model of alphaB-crystallin and provide a hypothesis for multimerization. A single particle cryo-EM reconstruction of a designed self-assembling octahedral protein oligomer showed that a designed protein assembly forms the predicted symmetry in solution. A 17 [angstrom] resolution single particle cryo-EM reconstruction of a mutant HCV IRES bound to the 40S ribosomal subunit revealed that deletion of three nucleotides in the IRES modulate interaction with the 40S ribosomal subunit. The conformational changes induced in the 40S were shown to affect a step after 80S formation, the transition from initiation to elongation. These studies illustrate the application of TEM to study the molecular mechanisms used by both membrane proteins and macromolecular complexes to carry out biological processes.
