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Strategies For De Novo Dna Sequencing
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Book Synopsis A Tale of Three Next Generation Sequencing Platforms by : Applied Research Press
Download or read book A Tale of Three Next Generation Sequencing Platforms written by Applied Research Press and published by Createspace Independent Publishing Platform. This book was released on 2015-07-24 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent's PGM, Pacific Biosciences' RS and the Illumina MiSeq. Here we compare the results obtained with those platforms to the performance of the Illumina HiSeq, the current market leader. In order to compare these platforms, and get sufficient coverage depth to allow meaningful analysis, we have sequenced a set of 4 microbial genomes with mean GC content ranging from 19.3 to 67.7%. Together, these represent a comprehensive range of genome content. Here we report our analysis of that sequence data in terms of coverage distribution, bias, GC distribution, variant detection and accuracy. All three fast turnaround sequencers evaluated here were able to generate usable sequence. However there are key differences between the quality of that data and the applications it will support. Proceeds from the sale of this book go to the support of an elderly disabled person.
Download or read book The Barley Genome written by Nils Stein and published by Springer. This book was released on 2018-08-18 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of the state-of-the-art in barley genome analysis, covering all aspects of sequencing the genome and translating this important information into new knowledge in basic and applied crop plant biology and new tools for research and crop improvement. Unlimited access to a high-quality reference sequence is removing one of the major constraints in basic and applied research. This book summarizes the advanced knowledge of the composition of the barley genome, its genes and the much larger non-coding part of the genome, and how this information facilitates studying the specific characteristics of barley. One of the oldest domesticated crops, barley is the small grain cereal species that is best adapted to the highest altitudes and latitudes, and it exhibits the greatest tolerance to most abiotic stresses. With comprehensive access to the genome sequence, barley’s importance as a genetic model in comparative studies on crop species like wheat, rye, oats and even rice is likely to increase.
Download or read book Flow Cytogenetics written by and published by Academic Press. This book was released on 2012-12-02 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to be devoted entirely to the application and development of flow techniques in cytogenetics. It provides comprehensive information on the use of flow cytometry and sorting for chromosome classification and purification. Cytogenetics and molecular biologists will find this book an invaluable reference source. - Practical details for the preparation and analysis of chromosomes using flow cytometry - Flow karyotyping for sensitive rapid analysis of chromosome normality and the detection of aberrant chromosomes - Flow sorting as a source of chromosome-specific DNA for gene mapping and recombinant DNA libraries - Construction and current status of chromosome-specific recombinant DNA libraries
Book Synopsis Bioinformatics in the Era of Post Genomics and Big Data by : Ibrokhim Y. Abdurakhmonov
Download or read book Bioinformatics in the Era of Post Genomics and Big Data written by Ibrokhim Y. Abdurakhmonov and published by BoD – Books on Demand. This book was released on 2018-06-20 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioinformatics has evolved significantly in the era of post genomics and big data. Huge advancements were made toward storing, handling, mining, comparing, extracting, clustering and analysis as well as visualization of big macromolecular data using novel computational approaches, machine and deep learning methods, and web-based server tools. There are extensively ongoing world-wide efforts to build the resources for regional hosting, organized and structured access and improving the pre-existing bioinformatics tools to efficiently and meaningfully analyze day-to-day increasing big data. This book intends to provide the reader with updates and progress on genomic data analysis, data modeling and network-based system tools.
Download or read book Man and Wildfowl written by Janet Kear and published by A&C Black. This book was released on 2010-11-30 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: The involvement of humans with ducks, geese and swans has probably been closer than with any other group of birds, today and for several millenia past. This involvement, in its many aspects, is the theme of this compelling and readable account by an Assistant Director of the Wildfowl and Wetlands Trust. Dr Kear ranges widely, from a summary of the taxonomy and natural history of wildfowl, through a history of domestication world wide, to wildfowling, decoys, conservation and captive breeding, conflicts with agriculture, and wildfowl in legend and literature. Throughout, the text abounds with little-known facts and insights to intrigue the general reader and expert alike - a reflection of the author's wide reading and affection for her subject. Jacket illustrations by Joe Blossom.
Book Synopsis Next Generation Sequencing by : Jerzy Kulski
Download or read book Next Generation Sequencing written by Jerzy Kulski and published by BoD – Books on Demand. This book was released on 2016-01-14 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.
Book Synopsis Next Generation Sequencing and Sequence Assembly by : Ali Masoudi-Nejad
Download or read book Next Generation Sequencing and Sequence Assembly written by Ali Masoudi-Nejad and published by Springer Science & Business Media. This book was released on 2013-07-09 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this book is to introduce the biological and technical aspects of next generation sequencing methods, as well as algorithms to assemble these sequences into whole genomes. The book is organized into two parts; part 1 introduces NGS methods and part 2 reviews assembly algorithms and gives a good insight to these methods for readers new to the field. Gathering information, about sequencing and assembly methods together, helps both biologists and computer scientists to get a clear idea about the field. Chapters will include information about new sequencing technologies such as ChIp-seq, ChIp-chip, and De Novo sequence assembly.
Book Synopsis Flower Breeding and Genetics by : Neil O. Anderson
Download or read book Flower Breeding and Genetics written by Neil O. Anderson and published by Springer Science & Business Media. This book was released on 2007-10-04 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: Floriculture is one of the fastest-growing sectors of commercial agriculture. This book provides a unique and valuable resource on the many issues and challenges facing flower breeders, as well as the industry at-large. Featuring contributions from 32 international authorities, it offers tools and directions for future crop domestication and enhancement as well as offers essential information for breeding a wide range of floriculture crops.
Book Synopsis Next-Generation Sequencing Data Analysis by : Xinkun Wang
Download or read book Next-Generation Sequencing Data Analysis written by Xinkun Wang and published by CRC Press. This book was released on 2016-04-06 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Practical Guide to the Highly Dynamic Area of Massively Parallel SequencingThe development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphi
Author :Naiara Rodríguez-Ezpeleta Publisher :Springer Science & Business Media ISBN 13 :1461407826 Total Pages :258 pages Book Rating :4.4/5 (614 download)
Book Synopsis Bioinformatics for High Throughput Sequencing by : Naiara Rodríguez-Ezpeleta
Download or read book Bioinformatics for High Throughput Sequencing written by Naiara Rodríguez-Ezpeleta and published by Springer Science & Business Media. This book was released on 2011-10-26 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing is revolutionizing molecular biology. Owing to this new technology it is now possible to carry out a panoply of experiments at an unprecedented low cost and high speed. These go from sequencing whole genomes, transcriptomes and small non-coding RNAs to description of methylated regions, identification protein – DNA interaction sites and detection of structural variation. The generation of gigabases of sequence information for each of this huge bandwidth of applications in just a few days makes the development of bioinformatics applications for next generation sequencing data analysis as urgent as challenging.
Book Synopsis Modern Biocatalysis by : Gavin Williams
Download or read book Modern Biocatalysis written by Gavin Williams and published by Royal Society of Chemistry. This book was released on 2018-05-31 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: The synergy between synthetic biology and biocatalysis is emerging as an important trend for future sustainable processes. This book reviews all modern and novel techniques successfully implemented in biocatalysis, in an effort to provide better performing enzymatic systems and novel biosynthetic routes to (non-)natural products. This includes the use of molecular techniques in protein design and engineering, construction of artificial metabolic pathways, and application of computational methods for enzyme discovery and design. Stress is placed on current ‘hot’ topics in biocatalysis, where recent advances in research are defining new grounds in enzyme-catalyzed processes. With contributions from leading academics around the world, this book makes a ground-breaking contribution to this progressive field and is essential reading for graduates and researchers investigating (bio)catalysis, enzyme engineering, chemical biology, and synthetic biology.
Book Synopsis Algorithms for Next-Generation Sequencing Data by : Mourad Elloumi
Download or read book Algorithms for Next-Generation Sequencing Data written by Mourad Elloumi and published by Springer. This book was released on 2017-09-18 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 14 contributed chapters in this book survey the most recent developments in high-performance algorithms for NGS data, offering fundamental insights and technical information specifically on indexing, compression and storage; error correction; alignment; and assembly. The book will be of value to researchers, practitioners and students engaged with bioinformatics, computer science, mathematics, statistics and life sciences.
Download or read book Gene Prediction written by Martin Kollmar and published by Humana Press. This book was released on 2019-05-19 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces software used for gene prediction with focus on eukaryotic genomes. The chapters in this book describe software and web server usage as applied in common use-cases, and explain ways to simplify re-annotation of long available genome assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary computational requirements, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Gene Prediction: Methods and Protocols is a valuable resource for researchers and research groups working on the assembly and annotation of single species or small groups of species. Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
Download or read book The Pangenome written by Hervé Tettelin and published by Springer Nature. This book was released on 2020-04-30 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book offers the first comprehensive account of the pan-genome concept and its manifold implications. The realization that the genetic repertoire of a biological species always encompasses more than the genome of each individual is one of the earliest examples of big data in biology that opened biology to the unbounded. The study of genetic variation observed within a species challenges existing views and has profound consequences for our understanding of the fundamental mechanisms underpinning bacterial biology and evolution. The underlying rationale extends well beyond the initial prokaryotic focus to all kingdoms of life and evolves into similar concepts for metagenomes, phenomes and epigenomes. The book’s respective chapters address a range of topics, from the serendipitous emergence of the pan-genome concept and its impacts on the fields of microbiology, vaccinology and antimicrobial resistance, to the study of microbial communities, bioinformatic applications and mathematical models that tie in with complex systems and economic theory. Given its scope, the book will appeal to a broad readership interested in population dynamics, evolutionary biology and genomics.
Book Synopsis Applications of RNA-Seq and Omics Strategies by : Fabio Marchi
Download or read book Applications of RNA-Seq and Omics Strategies written by Fabio Marchi and published by BoD – Books on Demand. This book was released on 2017-09-13 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The large potential of RNA sequencing and other "omics" techniques has contributed to the production of a huge amount of data pursuing to answer many different questions that surround the science's great unknowns. This book presents an overview about powerful and cost-efficient methods for a comprehensive analysis of RNA-Seq data, introducing and revising advanced concepts in data analysis using the most current algorithms. A holistic view about the entire context where transcriptome is inserted is also discussed here encompassing biological areas with remarkable technological advances in the study of systems biology, from microorganisms to precision medicine.
Book Synopsis The Collembola of Fennoscandia and Denmark, Part II: Entomobryomorpha and Symphypleona by : Arne Fjellberg
Download or read book The Collembola of Fennoscandia and Denmark, Part II: Entomobryomorpha and Symphypleona written by Arne Fjellberg and published by BRILL. This book was released on 2007-08-31 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume completes the survey of the ca. 400 species of springtails, which can be found in Finland, Sweden, Norway, Denmark, Iceland, the Faroes and the Arctic Islands and includes the sections Entomobryomorpha and Symphypleona. The first volume, published in 1998, covered Poduromorpha. Identification keys and full descriptions of the species are richly illustrated by line drawings. Photos are provided for some species displaying characteristic patterns of pigmentation. New diagnostic characters, including sensillary chaetotaxy and details of the mouth apparatus, are introduced. With the appearence of this book soil scientists and the interested amateur have now a modern tool to identify all species of Nordic springtails. In addition the habitat preferences and geographical distribuition are summarised. The book will be of general interest to everyone working on springtail identification.
Book Synopsis The Rubber Tree Genome by : Minami Matsui
Download or read book The Rubber Tree Genome written by Minami Matsui and published by Springer. This book was released on 2021-05-04 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the first comprehensive compilation of genome research on the Hevea brasiliensis rubber tree. The genomes of Hevea tree clones (cultivars) are described by three major international groups. Chapters on omics-driven investigations address a broad range of topics including genome annotation and utilisation, transcriptome and gene family analysis, genetic mapping, metabolic pathways in latex and molecular breeding. Additionally, an overview of fundamental rubber biology, especially on laticifers, provides a historical background that is relevant to rubber genome analysis. The book concludes with several perspectives on the future needs of rubber investigations and prospects of rubber genomics. Given the scope of topics, this book will appeal to researchers and university students working in genomics and biotechnology of the rubber tree, and to rubber breeders with an interest in non-conventional approaches to trait analysis, selection and breeding.
