Sperm Membrane Channels Receptors And Kinematics Using Boar Spermatozoa For Drug Toxicity Screening

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Sperm Membrane Channels, Receptors and Kinematics: Using boar spermatozoa for drug toxicity screening

Author : Alejandro Vicente Carrillo
Publisher : Linköping University Electronic Press
ISBN 13 : 9176857263
Total Pages : 82 pages
Book Rating : 4.1/5 (768 download)

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Book Synopsis Sperm Membrane Channels, Receptors and Kinematics: Using boar spermatozoa for drug toxicity screening by : Alejandro Vicente Carrillo

Download or read book Sperm Membrane Channels, Receptors and Kinematics: Using boar spermatozoa for drug toxicity screening written by Alejandro Vicente Carrillo and published by Linköping University Electronic Press. This book was released on 2016-10-11 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt: Internal fertilization usually implies that a spermatozoon, with intact attributes for zygote formation, passes all hurdles during its transport through the female genitalia and reaches the oocyte. During this journey, millions to billions of other spermatozoa perish. Spermatozoa are highly differentiated motile cells without synthetic capabilities. They generate energy via glycolysis and oxidative phosphorylation to sustain motility and to maintain the stability and functionality of their plasma membrane. In vivo, they spend their short lifespan bathing in female genital tract fluids of different origins, or are in vitro exposed to defined media during diverse sperm handling i.e. extension, cryopreservation, in vitro fertilization, etc. Being excitable cells, spermatozoa respond in vivo to various stimuli during pre-fertilization (capacitation, hyperactivation, oocyte location) and fertilization (acrosome reaction, interaction with the oocyte) events, mediated via diverse membrane ion-conducting channels and ligand-gated receptors. The present Thesis has mapped the presence and reactivity (sperm intactness and kinematics) of selected receptors, water and ion channels in ejaculated boar spermatozoa. The final aim was to find a relevant alternative cell type for in vitro bioassays that could ease the early scrutiny of candidate drugs as well as decreasing our needs for experimental animals according to the 3R principles. Spermatozoa are often extended, cooled and thawed to warrant their availability as fertile gametes for breeding or in vitro testing. Such manipulations stress the cells via osmotic variations and hence spermatozoa need to maintain membrane intactness by controlling the exchange of water and the common cryoprotectant glycerol, via aquaporins (AQPs). Both AQPs-7 and -9 were studied for membrane domain changes in cauda- and ejaculated spermatozoa (un-processed, extended, chilled or frozen-thawed). While AQP-9 maintained location through source and handling, thawing of ejaculated spermatozoa clearly relocated the labelling of AQP-7, thus appearing as a relevant marker for non-empirical studies of sperm cryopreservation. Alongside water, spermatozoa interact with calcium (Ca2+) via the main Ca2+ sperm channel CatSper. Increments in intracellular Ca2+ initiate motility hyperactivation and the acrosome reaction. The four subunits of the CatSper channel were present in boar spermatozoa, mediating changes in sperm motility under in vitro capacitation-inducing conditions (increased extracellular Ca2+ availability and bicarbonate) or challenge by the CatSper antagonists mibefradil and NNC 55-0396. Uterine and oviduct fluids are richest in endogenous opioids as ?-endorphins during mating and ovulation. Both ?- and ?- opioid receptors were present in boar spermatozoa modulating sperm motility, as in vitro challenge with known agonists (?: morphine; ?: DPDPE and ?: U 50488) and antagonists (?: naloxone; ?: naltrindole and ?: nor-binaltrorphimine) showed that the ?-opioid receptor maintained or increased motility while the ?-opioid receptor mediated decreased motility over time. Finally, boar spermatozoa depicted dose-response effects on sperm kinematics and mitochondrial potential following in vitro challenge with 130 pharmacological drugs and toxic compounds as well as with eight known mito-toxic compounds. In conclusion, boar spermatozoa expressing functional water (AQPs-7 and -9) and ion (CatSper 1-4) channels as well as ?- and ?-opioid receptors are able to adapt to stressful environmental variations, capacitation and pharmacological compounds and drug components. Ejaculated sperm suspensions are easily and painlessly obtained from breeding boars, and are suitable biosensors for in vitro drug-induced testing, complying with the 3R principles of reduction and replacement of experimental animals, during early toxicology screening.

Encyclopedia of Reproduction

Author : Ernst Knobil
Publisher : Academic Press
ISBN 13 :
Total Pages : 1160 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Encyclopedia of Reproduction by : Ernst Knobil

Download or read book Encyclopedia of Reproduction written by Ernst Knobil and published by Academic Press. This book was released on 1998-10-23 with total page 1160 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The present work should serve as a convenient and comprensive source of information encompassing all aspects of the subject of reproduction as it relates to the entire animal kingdom." -- Preface, p. xxxiii.

Spermatogenesis

Author : Lori Barnard
Publisher : Humana Press
ISBN 13 : 9781627030373
Total Pages : 0 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Spermatogenesis by : Lori Barnard

Download or read book Spermatogenesis written by Lori Barnard and published by Humana Press. This book was released on 2012-09-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.

Oxidants, Antioxidants, and Impact of the Oxidative Status in Male Reproduction

Author : Ralf Henkel
Publisher : Academic Press
ISBN 13 : 0128125675
Total Pages : 322 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Oxidants, Antioxidants, and Impact of the Oxidative Status in Male Reproduction by : Ralf Henkel

Download or read book Oxidants, Antioxidants, and Impact of the Oxidative Status in Male Reproduction written by Ralf Henkel and published by Academic Press. This book was released on 2018-08-23 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction is an essential reference for fertility practitioners and research and laboratory professionals interested in learning about the role of reactive oxygen species in sperm physiology and pathology. The book focuses on unravelling the pathophysiology of oxidative stress mediated male infertility, recruiting top researchers and clinicians to contribute chapters. This collection of expertise delves into the physico-chemical aspects of oxidative stress, including a new focus on reductive stress. Furthermore, the inclusion of clinical techniques to determine oxidative stress and the OMICS of reductive oxidative stress are also included. This is a must-have reference in the area of oxidative stress and male reproductive function. Offers comprehensive information on oxidative stress and its role in male reproduction, including new therapeutic approaches Deals with current approaches to oxidative stress using OMICS platform“/li> Designed for fertility practitioners, reproductive researchers, and laboratory professionals interested in learning about the role of reactive oxygen species in sperm physiology and pathology

Reproductive Immunology

Author : Satish Kumar Gupta
Publisher : Springer Science & Business Media
ISBN 13 : 9780792359234
Total Pages : 430 pages
Book Rating : 4.3/5 (592 download)

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Book Synopsis Reproductive Immunology by : Satish Kumar Gupta

Download or read book Reproductive Immunology written by Satish Kumar Gupta and published by Springer Science & Business Media. This book was released on 1999 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Papers presented at the VII International Congress of Reproductive Immunology held from 27-30 October 1998, New Delhi ; organised by National Institute of Immunology.

Andrology

Author : Eberhard Nieschlag
Publisher : Springer Science & Business Media
ISBN 13 : 3540783555
Total Pages : 630 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Andrology by : Eberhard Nieschlag

Download or read book Andrology written by Eberhard Nieschlag and published by Springer Science & Business Media. This book was released on 2010-01-13 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: The decade that has passed since publication of the second edition of this textbook has not only witnessed a tremendous increase in knowledge within the ? eld of and- logy, but also seen the ? eld itself achieve a newfound status within the medical p- fession. Knowledge and status have been of mutual bene? t to the ? eld and the growing critical mass of diagnostic and therapeutic possibilities have caused andrology to be recognized as a medical subspecialty in some countries such as Germany, Poland, and Estonia. The European Academy of Andrology (EAA) served as a pacemaker for this development and continues to strive for establishment of andrology as a clinical ? eld. Well-designed curricula and qualifying examinations have contributed to the of? cial recognition of andrology as a speciality. This recognition of the ? eld helps patients with andrological problems to ? nd the specialist they seek. This textbook summarizes the current state of knowledge in the ? eld of andrology. It is a source of knowledge to all those who are or want to become andrologists. In addition, as andrology is clearly an interdisciplinary ? eld, this book may serve as a compendium and source of reference for all those physicians and biologists active in neighboring areas, who want to obtain an overview of andrology and who require information on special problems. The extensive references are timely and up to date.

Innovations in Biomedical Engineering

Author : Marek Gzik
Publisher : Springer Nature
ISBN 13 : 3030991121
Total Pages : 341 pages
Book Rating : 4.0/5 (39 download)

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Book Synopsis Innovations in Biomedical Engineering by : Marek Gzik

Download or read book Innovations in Biomedical Engineering written by Marek Gzik and published by Springer Nature. This book was released on 2022-05-31 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest developments in the field of biomedical engineering and includes practical solutions and strictly scientific considerations. The development of new methods of treatment, advanced diagnostics or personalized rehabilitation requires close cooperation of experts from many fields, including, among others, medicine, biotechnology and finally biomedical engineering. The latter, combining many fields of science, such as computer science, materials science, biomechanics, electronics not only enables the development and production of modern medical equipment, but also participates in the development of new directions and methods of treatment. The presented monograph is a collection of scientific papers on the use of engineering methods in medicine. The topics of the work include both practical solutions and strictly scientific considerations expanding knowledge about the functioning of the human body. We believe that the presented works will have an impact on the development of the field of science, which is biomedical engineering, constituting a contribution to the discussion on the directions of development of cooperation between doctors, physiotherapists and engineers. We would also like to thank all the people who contributed to the creation of this monograph—both the authors of all the works and those involved in technical works.

8th European Medical and Biological Engineering Conference

Author : Tomaz Jarm
Publisher : Springer Nature
ISBN 13 : 3030646106
Total Pages : 1198 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis 8th European Medical and Biological Engineering Conference by : Tomaz Jarm

Download or read book 8th European Medical and Biological Engineering Conference written by Tomaz Jarm and published by Springer Nature. This book was released on 2020-11-29 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims at informing on new trends, challenges and solutions, in the multidisciplinary field of biomedical engineering. It covers traditional biomedical engineering topics, as well as innovative applications such as artificial intelligence in health care, tissue engineering , neurotechnology and wearable devices. Further topics include mobile health and electroporation-based technologies, as well as new treatments in medicine. Gathering the proceedings of the 8th European Medical and Biological Engineering Conference (EMBEC 2020), held on November 29 - December 3, 2020, in Portorož, Slovenia, this book bridges fundamental and clinically-oriented research, emphasizing the role of education, translational research and commercialization of new ideas in biomedical engineering. It aims at inspiring and fostering communication and collaboration between engineers, physicists, biologists, physicians and other professionals dealing with cutting-edge themes in and advanced technologies serving the broad field of biomedical engineering.

Male Infertility

Author : Anne M. Jequier
Publisher : John Wiley & Sons
ISBN 13 : 0470695269
Total Pages : 386 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Male Infertility by : Anne M. Jequier

Download or read book Male Infertility written by Anne M. Jequier and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book deals almost wholly with the clinical aspects of the subject. Male Infertility is a book written by a clinician for clinicians, most frequently gynaecologists who are battling to understand reproductive pathology in the infertile male patient. It is intended to familiarize the gynaecologist and other interested clinicians in the clinical management of an infertile man and provide that individual with practical guidance to a clinical problem.

Cockroaches

Author : William J. Bell
Publisher : JHU Press
ISBN 13 : 0801891752
Total Pages : 781 pages
Book Rating : 4.8/5 (18 download)

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Book Synopsis Cockroaches by : William J. Bell

Download or read book Cockroaches written by William J. Bell and published by JHU Press. This book was released on 2007-07-27 with total page 781 pages. Available in PDF, EPUB and Kindle. Book excerpt: The essential volume on the biology and behavior of these remarkable insects. “This transformative work will be an inspiration to students of entomology.” —Choice The cockroach is truly an evolutionary wonder. This definitive volume provides a complete overview of suborder Blattaria, highlighting the diversity of these amazing insects in their natural environments. Beginning with a foreword by Edward O. Wilson, the book explores the fascinating natural history and behavior of cockroaches, describing their various colors, sizes, and shapes, as well as how they move on land, in water, and through the air. In addition to habitat use, diet, reproduction, and behavior, Cockroaches covers aspects of cockroach biology, such as the relationship between cockroaches and microbes, termites as social cockroaches, and the ecological impact of the suborder. With over 100 illustrations, an expanded glossary, and an invaluable set of references, this work is destined to become the classic book on the Blattaria. Students and research entomologists can mine each chapter for new ideas, new perspectives, and new directions for future study. “Well-written . . . visually attractive . . . This book is much needed to educate biologists about the fascinating biology and diversity of cockroaches.” —Integrative and Comparative Biology “A must-have for any insect hobbyest.” —Allpet Roaches Forum “This contribution is an important source of information on cockroach natural history and diversity.” —The Quarterly Review of Biology “Suitable for researchers, students, and naturalists, chapters are topical, exploring the diversity of cockroaches.” —Southeastern Naturalist

Practical Laboratory Andrology

Author : David Mortimer
Publisher : Oxford University Press, USA
ISBN 13 : 9780195065954
Total Pages : 424 pages
Book Rating : 4.0/5 (659 download)

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Book Synopsis Practical Laboratory Andrology by : David Mortimer

Download or read book Practical Laboratory Andrology written by David Mortimer and published by Oxford University Press, USA. This book was released on 1994 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical manual on sperm analysis presents the diagnostic and therapeutic procedures that are used in andrology laboratories to analyze and assess male infertility. Diagnostic areas include: semen analysis and the biochemical, immunological and microbiological examination of human semen and spermatozoa; computer-aided sperm motility analysis; sperm ultrastructure; and assessment of sperm transport through the female tract and sperm fertilizing ability. The clinical relevance of various diagnostic procedures is also discussed. Therapeutic topics include sperm washing techniques, semen cryopreservation, and insemination procedures. The volume also covers safety in the andrology laboratory, technician training, and quality control. The text is extensively illustrated and will be an invaluable resource to all scientists and technicians who diagnose male infertility. It will also be of interest to researchers working in human gamete biology and reproductive physiology. The detailed methods described in the book are relevant to all hospital, commercial, and university laboratories involved in infertility diagnosis and treatment.

Design of Artificial Human Joints & Organs

Author : Subrata Pal
Publisher : Springer Science & Business Media
ISBN 13 : 146146255X
Total Pages : 429 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Design of Artificial Human Joints & Organs by : Subrata Pal

Download or read book Design of Artificial Human Joints & Organs written by Subrata Pal and published by Springer Science & Business Media. This book was released on 2013-08-31 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Design of Artificial Human Joints & Organs is intended to present the basics of the normal systems and how, due to aging, diseases or trauma, body parts may need to be replaced with manmade materials. The movement of the body generates forces in various work situations and also internally at various joints, muscles and ligaments. It is essential to figure out the forces, moments, pressure etc to design replacements that manage these stresses without breaking down. The mechanical characterization of the hard and the soft tissues are presented systematically using the principles of solid mechanics. The viscoelastic properties of the tissue will also discussed. This text covers the design science and methodology from concept to blueprint to the final component being replaced. Each chapter will be a brief overview of various joint/organ replacement systems. Engineers working on artificial joints and organs, as well as students of Mechanical Engineering and Biomedical Engineering are the main intended audience, however, the pedagogy is simple enough for those who are learning the subject for the first time.

Sustainable Agriculture Reviews 54

Author : Vinod Kumar Yata
Publisher :
ISBN 13 : 9783030765309
Total Pages : 0 pages
Book Rating : 4.7/5 (653 download)

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Book Synopsis Sustainable Agriculture Reviews 54 by : Vinod Kumar Yata

Download or read book Sustainable Agriculture Reviews 54 written by Vinod Kumar Yata and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews concepts and recent advances of biotechnological approaches for livestock production. Indeed, biotechnologies have recently emerged as powerful tools for animal breeding, genetics, production, nutrition, and animal health. Applications to the production of livestock such as cattle, camel, and poultry are detailed. Chapters also present biotechnological applications for diagnostics, animal nutrition, and animal food production.

Encyclopedia of Forensic Sciences

Author :
Publisher : Academic Press
ISBN 13 : 0123821665
Total Pages : 2253 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Encyclopedia of Forensic Sciences by :

Download or read book Encyclopedia of Forensic Sciences written by and published by Academic Press. This book was released on 2012-12-28 with total page 2253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic science includes all aspects of investigating a crime, including: chemistry, biology and physics, and also incorporates countless other specialties. Today, the service offered under the guise of "forensic science’ includes specialties from virtually all aspects of modern science, medicine, engineering, mathematics and technology. The Encyclopedia of Forensic Sciences, Second Edition, Four Volume Set is a reference source that will inform both the crime scene worker and the laboratory worker of each other’s protocols, procedures and limitations. Written by leading scientists in each area, every article is peer reviewed to establish clarity, accuracy, and comprehensiveness. As reflected in the specialties of its Editorial Board, the contents covers the core theories, methods and techniques employed by forensic scientists – and applications of these that are used in forensic analysis. This 4-volume set represents a 30% growth in articles from the first edition, with a particular increase in coverage of DNA and digital forensics Includes an international collection of contributors The second edition features a new 21-member editorial board, half of which are internationally based Includes over 300 articles, approximately 10pp on average Each article features a) suggested readings which point readers to additional sources for more information, b) a list of related Web sites, c) a 5-10 word glossary and definition paragraph, and d) cross-references to related articles in the encyclopedia Available online via SciVerse ScienceDirect. Please visit www.info.sciencedirect.com for more information This new edition continues the reputation of the first edition, which was awarded an Honorable Mention in the prestigious Dartmouth Medal competition for 2001. This award honors the creation of reference works of outstanding quality and significance, and is sponsored by the RUSA Committee of the American Library Association

Electromagnetism and Life

Author : Robert O. Becker
Publisher :
ISBN 13 : 9780981854908
Total Pages : 216 pages
Book Rating : 4.8/5 (549 download)

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Book Synopsis Electromagnetism and Life by : Robert O. Becker

Download or read book Electromagnetism and Life written by Robert O. Becker and published by . This book was released on 2010-04 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The environment is now thoroughly polluted by man-made sources of electromagnetic radiation with frequencies and magnitudes never before present. Man's activities have probably changed the earth's electromagnetic background to a greater degree than they have changed any other natural physical attribute of the earth. The evidence now indicates that the present abnormal electromagnetic environment constitutes a significant health risk. There are also positive aspects of the relationship between electromagnetism and life. Clinical uses of electromagnetic energy are increasing and promise to expand into important areas in the near future. This book synthesizes the various aspects of the role of electricity in biology.

Male Reproduction and Fertility

Author : Andrés Negro-Vilar
Publisher : Lippincott Williams & Wilkins
ISBN 13 :
Total Pages : 416 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Male Reproduction and Fertility by : Andrés Negro-Vilar

Download or read book Male Reproduction and Fertility written by Andrés Negro-Vilar and published by Lippincott Williams & Wilkins. This book was released on 1983 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Masters Theses in the Pure and Applied Sciences

Author : Wade H. Shafer
Publisher : Springer
ISBN 13 :
Total Pages : 328 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Masters Theses in the Pure and Applied Sciences by : Wade H. Shafer

Download or read book Masters Theses in the Pure and Applied Sciences written by Wade H. Shafer and published by Springer. This book was released on 1985-03 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Masters Theses in the Pure and Applied Sciences was first conceived, published, and disseminated by the Center for Information and Numerical Data Analysis and Synthesis (CINDAS) * at Purdue University in 1 957, starting its coverage of theses with the academic year 1955. Beginning with Volume 13, the printing and dissemination phases of the activity were transferred to University Microfilms/Xerox of Ann Arbor, Michigan, with the thought that such an arrangement would be more beneficial to the academic and general scientific and technical community. After five years of this joint undertaking we had concluded that it was in the interest of all con cerned if the printing and distribution of the volumes were handled by an interna tional publishing house to assure improved service and broader dissemination. Hence, starting with Volume 18, Masters Theses in the Pure and Applied Sciences has been disseminated on a worldwide basis by Plenum Publishing Cor poration of New York, and in the same year the coverage was broadened to include Canadian universities. All back issues can also be ordered from Plenum. We have reported in Volume 28 (thesis year 1 983) a total of 10,661 theses titles from 26 Canadian and 197 United States universities. We are sure that this broader base for these titles reported will greatly enhance the value of this important annual reference work. While Volume 28 reports theses submitted in-1983, on occasion, certain univer sities do report theses submitted in previous years but not reported at the time.