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Spectral And Imaging Cytometry
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Book Synopsis Spectral and Imaging Cytometry by : Natasha S. Barteneva
Download or read book Spectral and Imaging Cytometry written by Natasha S. Barteneva and published by Springer Nature. This book was released on 2023-04-19 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Book Synopsis Imaging Flow Cytometry by : Natasha S. Barteneva
Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by Humana. This book was released on 2015-11-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Book Synopsis Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by : Anna Porwit
Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
Book Synopsis Recent Advances in Cytometry, Part A by :
Download or read book Recent Advances in Cytometry, Part A written by and published by Academic Press. This book was released on 2011-06-13 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays Discusses many new assays developed since the previous edition Presents recent developments in cytometric intrumentation/technology
Book Synopsis Flow Cytometry Protocols by : Teresa S. Hawley
Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Cytometry: Applications by :
Download or read book Advances in Cytometry: Applications written by and published by Elsevier. This book was released on 2024-05-03 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Cytometry: Applications, Volume 176 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Book Synopsis Recent Advances in Cytometry by : Zbigniew Darzynkiewicz
Download or read book Recent Advances in Cytometry written by Zbigniew Darzynkiewicz and published by Academic Press. This book was released on 2011 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays * Discusses many new assays developed since the previous edition. Presents recent developments in cytometric intrumentation/technology.
Book Synopsis Real-time Fourier Transform Spectrometry for Fluorescence Imaging and Flow Cytometry by :
Download or read book Real-time Fourier Transform Spectrometry for Fluorescence Imaging and Flow Cytometry written by and published by . This book was released on 1990 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt: We present a Fourier transform (FT) spectrometer that is suitable for real-time spectral analysis in fluorescence imaging and flow cytometry. The instrument consists of a novel type of interferometer that can be modulated at frequencies of up to 100 kHz and has a high light throughput; and a dedicated, parallel array processor for the real-time computation of spectral parameters. The data acquisition array processor can be programmed by a host computer to perform any desired linear transform on the interferogram and can thus separate contributions from multiple fluorescence microscopy. The integration of a flow cytometer and a spectral imaging fluorescence microscope is discussed, and the concepts of direct and reversed virtual sorting'' are introduced. 9 refs., 8 figs.
Book Synopsis Imaging Flow Cytometry by : Natasha S. Barteneva
Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by . This book was released on 2015 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionised our ability to analyse cells, cellular clusters and populations. Beginning with an introduction to technology, it continues with sections addressing protocols for studies on the cell nucleus and nucleic acids, FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.
Author :Alain Jaquemin-Sablon Publisher :Springer Science & Business Media ISBN 13 :364261115X Total Pages :246 pages Book Rating :4.6/5 (426 download)
Book Synopsis Flow and Image Cytometry by : Alain Jaquemin-Sablon
Download or read book Flow and Image Cytometry written by Alain Jaquemin-Sablon and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, "Membrane Dynamics and Function", deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.
Book Synopsis Advanced Optical Flow Cytometry by : Valery V. Tuchin
Download or read book Advanced Optical Flow Cytometry written by Valery V. Tuchin and published by John Wiley & Sons. This book was released on 2011-03-31 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.
Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu
Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia
Download or read book Flow Cytometry written by Marion G. Macey and published by Springer Science & Business Media. This book was released on 2007-11-03 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Book Synopsis Coherent Raman Scattering Microscopy by : Ji-Xin Cheng
Download or read book Coherent Raman Scattering Microscopy written by Ji-Xin Cheng and published by CRC Press. This book was released on 2016-04-19 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc
Book Synopsis Flow Cytometry by : Alice Longobardi Givan
Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Book Synopsis Fluorescence Applications in Biotechnology and Life Sciences by : Ewa M. Goldys
Download or read book Fluorescence Applications in Biotechnology and Life Sciences written by Ewa M. Goldys and published by John Wiley & Sons. This book was released on 2009-08-24 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro
Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
