Safety In Cell And Tissue Culture

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Safety in Cell and Tissue Culture

Author : G. Stacey
Publisher : Springer Science & Business Media
ISBN 13 : 940114916X
Total Pages : 257 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Safety in Cell and Tissue Culture by : G. Stacey

Download or read book Safety in Cell and Tissue Culture written by G. Stacey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cells and the detection of adven titious agents. However, it is clear that there is no room for complacency in this field and the recent expansion in the use of animal cells in the manufacture of medical products and the development of new biological assays for diagnostic and pharmaco-toxicological screening, underlines the need for vigilance regarding the correct and safe use of animal cells as substrates. This book is therefore very timely and should prove to be a highly valuable text, finding a wider audience beyond those with respon sibility for laboratory safety. The book guides the reader from fundamental cell biology issues and the establishment of new in vitro methods, through testing and validation of cell lines and on to issues in the use of animal cells in manufacturing processes.

Tissue Culture Techniques

Author : Bernice M. Martin
Publisher : Springer Science & Business Media
ISBN 13 : 1461202477
Total Pages : 251 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Tissue Culture Techniques by : Bernice M. Martin

Download or read book Tissue Culture Techniques written by Bernice M. Martin and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

General Techniques of Cell Culture

Author : Maureen A. Harrison
Publisher : Cambridge University Press
ISBN 13 : 9780521574969
Total Pages : 178 pages
Book Rating : 4.5/5 (749 download)

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Book Synopsis General Techniques of Cell Culture by : Maureen A. Harrison

Download or read book General Techniques of Cell Culture written by Maureen A. Harrison and published by Cambridge University Press. This book was released on 1997-10-13 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concise introduction to a major technique of cell biology laboratories for those new to the field.

Cell Culture Methods for In Vitro Toxicology

Author : G. Stacey
Publisher : Springer Science & Business Media
ISBN 13 : 9401709963
Total Pages : 153 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Cell Culture Methods for In Vitro Toxicology by : G. Stacey

Download or read book Cell Culture Methods for In Vitro Toxicology written by G. Stacey and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Culture Methods for in vitro Toxicology introduces the reader to a range of techniques involved in the use of in vitro cell culture in toxicological studies. It deals with major cell types studied in the field of toxicology and will be useful for anyone wishing to start work with animal cell cultures or to refresh their knowledge relating to in vitro cell models. Fundamental chapters deal with the general biology of cytotoxicity and cell immortalisation these are key issues for in vitro systems addressing the `3Rs' principle. Up-to-date overviews deal with the use of cells from liver, brain and intestine. In addition, biochemical analysis of cell responses, biotransformation pathways in cells and recombinant approaches to the early detection of cell stress are also covered in detail. Prominent features of in vitro technologies also include regulation, biosafety and standardisation. Dedicated chapters deal with these issues in a practical way in order to lead the reader to the right source of information. This book provides an up-to-date, informative and practical review of cell culture methods for in vitro toxicology. It will be of equal benefit to students and experienced toxicologists with little experience of in vitro cell culture.

Cell & Tissue Culture

Author : D. G. Newell
Publisher :
ISBN 13 :
Total Pages : 1760 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Cell & Tissue Culture by : D. G. Newell

Download or read book Cell & Tissue Culture written by D. G. Newell and published by . This book was released on 1993 with total page 1760 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and Tissue Culture provides a comprehensive collection of cell and tissue culture techniques and procedures to assist scientists with the labour intensive and expensive in vitro techniques. Specific techniques are included together with standard techniques with an Editorial Advisory Board ensuring that all procedures are written by research workers eminent in their discipline. Being a looseleaf format, the contents are easily updated, allowing for rapid dissemination of valuable revised and new techniques. In this way, information remains up-to-date, and will also expand to ensure that every topic of interest is covered. The manual should be considered an essential adjunct to any laboratory or library.

Animal Tissue Culture

Author : A Wilson Aruni
Publisher : MJP Publisher
ISBN 13 :
Total Pages : 219 pages
Book Rating : 4./5 ( download)

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Book Synopsis Animal Tissue Culture by : A Wilson Aruni

Download or read book Animal Tissue Culture written by A Wilson Aruni and published by MJP Publisher. This book was released on 2019-06-10 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and tissues culture has been one of the first and foremost techniques paving for recent cutting-edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. It has played a substantial role in the developments of health care and prophylactics industries, thus serving the mankind. It has made the dream of producing cost-effective prophylactics, diagnostics and therapeutics come true and affordable. In the recent past, with the explosion of knowledge in the field of biotechnology, intensive research in being carried out, where undergraduate and post-graduate courses are being offered in this field. Even through more emphasis is being given to theory, a dearth of practical knowledge is lacking due to paucity of established tissue culture facilities.

Freshney's Culture of Animal Cells

Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 1119513030
Total Pages : 832 pages
Book Rating : 4.1/5 (195 download)

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Book Synopsis Freshney's Culture of Animal Cells by : R. Ian Freshney

Download or read book Freshney's Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2021-02-17 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Tissue Culture

Author : Paul F. Jr. Kruse
Publisher : Elsevier
ISBN 13 : 0323142079
Total Pages : 896 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Tissue Culture by : Paul F. Jr. Kruse

Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Animal Cell Culture

Author : John M. Davis
Publisher : John Wiley & Sons
ISBN 13 : 0470975636
Total Pages : 389 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Animal Cell Culture by : John M. Davis

Download or read book Animal Cell Culture written by John M. Davis and published by John Wiley & Sons. This book was released on 2011-03-16 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology

Author : Jack G. Chirikjian
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780867208955
Total Pages : 220 pages
Book Rating : 4.2/5 (89 download)

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Book Synopsis Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology by : Jack G. Chirikjian

Download or read book Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology written by Jack G. Chirikjian and published by Jones & Bartlett Learning. This book was released on 1995 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vol. I: The work presented in these two volumes is the collaborative effort of over twenty undergraduate science faculty, whose common goal was to develop a text of unique and flexible laboratory activities focusing on the theory and practice of biotechnology for undergraduate students. The books are designed to provide flexibility for easy integration into any course in the life sciences with an experimental emphasis.

Cell and Tissue Culture

Author : John Paul
Publisher : Churchill Livingstone
ISBN 13 :
Total Pages : 472 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Cell and Tissue Culture by : John Paul

Download or read book Cell and Tissue Culture written by John Paul and published by Churchill Livingstone. This book was released on 1970 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic Cell Culture Protocols

Author : Jeffrey W. Pollard
Publisher : Springer Science & Business Media
ISBN 13 : 0896034410
Total Pages : 477 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard

Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Culture of Animal Cells

Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 0470649356
Total Pages : 766 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2011-02-02 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture

Cell Culture

Author : Alan Doyle
Publisher :
ISBN 13 :
Total Pages : 204 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Cell Culture by : Alan Doyle

Download or read book Cell Culture written by Alan Doyle and published by . This book was released on 1997-07-04 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides concise practical instructions for setting up a cell culture facility and operating it effectively and safely. Procedures are described to guide the readers from the initiation of primary cultures, handling established cell lines and quantifying their performance, through to the production of specialist cell types by immortalization. The user-friendly format makes Mammalian Cell Culture: Essential Techniques a convenient summary of techniques for experienced workers as well as an invaluable aid to tissue culture in nonspecialized environments. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

Plant Cell Culture Protocols

Author : Robert D. Hall
Publisher : Springer Science & Business Media
ISBN 13 : 1592595839
Total Pages : 422 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Cell Culture Protocols by : Robert D. Hall

Download or read book Plant Cell Culture Protocols written by Robert D. Hall and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

Contamination in Tissue Culture

Author : Jorgen Fogh
Publisher : Elsevier
ISBN 13 : 0323156983
Total Pages : 300 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Contamination in Tissue Culture by : Jorgen Fogh

Download or read book Contamination in Tissue Culture written by Jorgen Fogh and published by Elsevier. This book was released on 2012-12-02 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contamination in Tissue Culture covers the sources, prevention, detection, and elimination of contamination in tissue culture. Composed of 12 chapters, the book describes the frequency of occurrence of contamination and the many different effects of contamination on cultured cells. After introducing the intraspecies contamination of cell cultures, the book explains a specific type of contamination, such as bacterial, fungal, viral, and parasitic contamination. A chapter in this book describes the reversible and irreversible alterations of cultured FL human amnion cells after experimental mycoplasmal infection. Chapters 9 and 10 examine the occurrence of tissue culture contaminants by electron microscopy and procedures for isolating and identifying viral contaminants. The concluding chapter covers sterility tests of media and solutions for tissue culture and the use of antibiotics. It also summarizes the major developments made as well as future challenges in the field. This book will be helpful to investigators, teachers, students, and technicians within the many disciplines of cell biology, physiology, cytology, virology, immunology, genetics, oncology, molecular biology, biochemistry, and biophysics, in which tissue and cell cultures are used, either as the primary object of research or as tools.

Cell and Tissue Culture for Medical Research

Author : Alan Doyle
Publisher :
ISBN 13 :
Total Pages : 482 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Cell and Tissue Culture for Medical Research by : Alan Doyle

Download or read book Cell and Tissue Culture for Medical Research written by Alan Doyle and published by . This book was released on 2000-08-22 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and tissue culture is a technique in which plant or animal cells are grown under controlled conditions in the laboratory. This is then used for the analysis of the cells themselves, the assessment of the cell's response to chemicals, or as a tool to produce cellular-derived protein products. This book is a collection of fundamental and specific applied procedures in cell and tissue culture which form the basis of the new medical techniques of tissue engineering and gene therapy. It combines both detailed laboratory procedures and informative overviews. * Provides step-by-step protocols with troubleshooting tips and notes on time considerations. * Main procedures are supplemented by alternative procedures, background information and references. * Experimental examples indicate expected results.