Author : Donald S. Anson
Publisher : Springer Science & Business Media
ISBN 13 : 158829739X
Total Pages : 165 pages
Book Rating : 4.5/5 (882 download)
Book Synopsis Reporter Genes by : Donald S. Anson
Download or read book Reporter Genes written by Donald S. Anson and published by Springer Science & Business Media. This book was released on 2007-09-13 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.