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Proteins At Solid Liquid Interfaces
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Book Synopsis Proteins at Solid-Liquid Interfaces by : Philippe Déjardin
Download or read book Proteins at Solid-Liquid Interfaces written by Philippe Déjardin and published by Springer Science & Business Media. This book was released on 2006-09-28 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Book Synopsis Proteins at Solid-liquid Interfaces by : Ananthakrishnan G. Sethuraman
Download or read book Proteins at Solid-liquid Interfaces written by Ananthakrishnan G. Sethuraman and published by . This book was released on 2004 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Behavior of Proteins at Solid-liquid Interfaces by : Adam Till Garland
Download or read book The Behavior of Proteins at Solid-liquid Interfaces written by Adam Till Garland and published by . This book was released on 2014 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: The behavior of a protein molecule at the solid-liquid interface is a worthy scientific problem for at least three reasons. The main driving force for studying this problem is a practical one, as many areas of bio-related technologies, such as medical implants, biosensing, and drug delivery, require the understanding of protein-surface interactions. In this dissertation, the nature of the precursive weakly adsorbed state of proteins during binding is reviewed. From this perspective, the adsorption and binding of proteins to a solid block copolymer thin film was achieved with regular spacing. Further efforts produced a monolayer of green fluorescent protein (GFP) covalently bound with regular spacing and orientation to a diblock copolymer thin film. This protein could be folded and refolded by changing solvent characteristics. We also explored the binding of DC-SIGN to mannose and mannotriose bearing lipid membranes. While no binding was observed, the usefulness of the lipid-based glycan microarray was proven using the well-studied CTB-GM1 binding motif.
Book Synopsis Proteins at Liquid Interfaces by : D. Möbius
Download or read book Proteins at Liquid Interfaces written by D. Möbius and published by Elsevier. This book was released on 1998-04-24 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interfacial behaviour of surfactants and proteins, and their mixtures, is of importance in a wide range of areas such as food technology, detergency, cosmetics, coating processes, biomedicine, pharmacy and biotechnology. Methods such as surface and interfacial tension measurements and interfacial dilation and shear rheology characterise the relationships between these interfacial properties and the complex behaviour of foams and emulsions is established. Recently-developed experimental techniques, such as FRAP which enable the measurement of molecular mobility in adsorption layers, are covered in this volume. The development of theories to describe the thermodynamic surface state or the exchange of matter for proteins and protein/surfactant mixtures is also described. Features of this book: • Reflects the state-of-the-art research and application of protein interfacial layers rather than a snapshot of only some recent developments. • Emphasis is placed on experimental details as well as recent theoretical developments. • New experimental techniques applied to protein interfacial layers are described, such as FRAP or ADSA, or rheological methods to determine the mechanical behaviour of protein-modified interfaces. • A large number of practical applications, ranging from emulsions relevant in food technology for medical problems such as lung surfactants, to the characterisation of foams intrinsic to beer and champagne production. The book will be of interest to research and university institutes dedicated to interfacial studies in chemistry, biology, pharmacy, medicine and food engineering. Industrial departments for research and technology in food industry, pharmacy, medicine and brewery research will also find this volume of value.
Book Synopsis Protein Adsorption at Solid-liquid Interfaces by : Gaurav Anand
Download or read book Protein Adsorption at Solid-liquid Interfaces written by Gaurav Anand and published by . This book was released on 2010 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Selected Experiments with Proteins at Solid-liquid Interfaces by : Jonathan Teichroeb
Download or read book Selected Experiments with Proteins at Solid-liquid Interfaces written by Jonathan Teichroeb and published by . This book was released on 2008 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes a number of novel experiments contributing to the understanding of protein adsorption from both a fundamental and applied perspective. The first three papers involve the use of the localized surface plasmon resonance of gold nanospheres to measure protein conformational dependencies during heat and acid denaturation. A significant finding of all three studies is that the state and stability of BSA depends strongly upon local curvature. The last two papers investigate protein adsorption relevant to the biomaterial field. A unique fibril-like morphology is identified on galyfilcon A. Protein morphology is discussed in terms of bare lens morphology, roughness, and surface composition.
Book Synopsis Protein Adsorption at Solid-liquid Interfaces by : Hendrik Sibolt van Damme
Download or read book Protein Adsorption at Solid-liquid Interfaces written by Hendrik Sibolt van Damme and published by . This book was released on 1990 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Two Dimensional Protein Crystallization at Solid Liquid Interfaces by : Tammy Lynn Calvert
Download or read book Two Dimensional Protein Crystallization at Solid Liquid Interfaces written by Tammy Lynn Calvert and published by . This book was released on 1997 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Adsorption at the Solid/liquid Interface by : Richard James Marsh
Download or read book Protein Adsorption at the Solid/liquid Interface written by Richard James Marsh and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Adsorption of Proteins and Interactions with Surfactants at the Solid/liquid Interface by : Marie Wahlgren
Download or read book Adsorption of Proteins and Interactions with Surfactants at the Solid/liquid Interface written by Marie Wahlgren and published by . This book was released on 1992 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Competitive Adsorption of Proteins at Solid-liquid Interfaces by : Nicoletta Giamblanco
Download or read book Competitive Adsorption of Proteins at Solid-liquid Interfaces written by Nicoletta Giamblanco and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Reflectometric Studies of Protein Interactions at Solid-liquid Interfaces by : Mirjam Oldenzeel
Download or read book Reflectometric Studies of Protein Interactions at Solid-liquid Interfaces written by Mirjam Oldenzeel and published by . This book was released on 1993 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Aggregation at Solid/liquid Interfaces: a Monte Carlo Study with Explicit and Implicit Solvent Effects by : Rehman Fazeem
Download or read book Protein Aggregation at Solid/liquid Interfaces: a Monte Carlo Study with Explicit and Implicit Solvent Effects written by Rehman Fazeem and published by . This book was released on 2013 with total page 185 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Metropolis Monte Carlo (MC) simulations were performed with positively charged peptides in aqueous solution to study changes in peptide conformations at solid/liquid interface, and its effects on protein aggregation. Intermediate-resolution diblock model peptide, comprising of 10 units of ALA (non-polar) and LYS (polar) amino acid residues, was used for the simulations. In the first approach to modeling, solvent effects were considered explicitly. The explicit model was then used to study two peptide molecules, in helical structure, at solid/liquid interface. In order to increase the number of peptide molecules in the simulation box, with reduced computational cost, an implicit solvent model was developed with nonadditive hydrogen bonding and hydrophobic interaction potentials. The implicit model was used to simulate two peptides of helical structure at charged surfaces (to compare with the explicit model), and ten peptides of random coil structure with and without charged surfaces. The peptides were observed to always move towards the negatively charged surface and orient with residues of complimentary charge settling close to the surface, maximizing the electrostatic interactions. On reaching the surface, the peptides partially lose their secondary structure and clusters around the hydrophobic ends; this restructuring and dehydration of the peptides provides the entropic drive for adsorption and subsequent misfolding events. The 2- peptide-water-surface system in explicit model was also simulated with periodic switching of surface charge polarity, to induce a "shaking effect" in order to observe possible peptide configurational changes"--Abstract, page iii.
Download or read book Proteins at Interfaces II written by and published by . This book was released on 1995 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fundamentals of Protein Adsorption at the Solid-Liquid Interface Over Short Time Periods by : Brad Hall
Download or read book Fundamentals of Protein Adsorption at the Solid-Liquid Interface Over Short Time Periods written by Brad Hall and published by . This book was released on 2014 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purpose: To directly measure the quantity and degree of denaturation of biomaterial- adsorbed proteins over short time periods.
Book Synopsis Protein Instability at Interfaces During Drug Product Development by : Jinjiang Li
Download or read book Protein Instability at Interfaces During Drug Product Development written by Jinjiang Li and published by Springer Nature. This book was released on 2021-02-12 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are exposed to various interfacial stresses during drug product development. They are subjected to air-liquid, liquid-solid, and, sometimes, liquid-liquid interfaces throughout the development cycle-from manufacturing of drug substances to storage and drug delivery. Unlike small molecule drugs, proteins are typically unstable at interfaces where, on adsorption, they often denature and form aggregates, resulting in loss of efficacy and potential immunogenicity. This book covers both the fundamental aspects of proteins at interfaces and the quantification of interfacial behaviors of proteins. Importantly, this book introduces the industrial aspects of protein instabilities at interfaces, including the processes that introduce new interfaces, evaluation of interfacial instabilities, and mitigation strategies. The audience that this book targets encompasses scientists in the pharmaceutical and biotech industry, as well as faculty and students from academia in the surface science, pharmaceutical, and medicinal chemistry areas.
Book Synopsis Imaging of Protein Adsorption and Self-assembled Proteins at the Solid/liquid Interface by Atomic Force Microscopy by : David Tae Kim
Download or read book Imaging of Protein Adsorption and Self-assembled Proteins at the Solid/liquid Interface by Atomic Force Microscopy written by David Tae Kim and published by . This book was released on 2004 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
