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Protein Phosphatase Protocols
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Book Synopsis Protein Phosphatase Protocols by : Greg Moorhead
Download or read book Protein Phosphatase Protocols written by Greg Moorhead and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics.
Book Synopsis Protein Phosphatase Protocols by : Greg Moorhead
Download or read book Protein Phosphatase Protocols written by Greg Moorhead and published by Humana Press. This book was released on 2006-09-16 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics.
Book Synopsis The Protein Protocols Handbook by : John M. Walker
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2002 with total page 1446 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Book Synopsis Phosphatase Modulators by : José Luis Millán
Download or read book Phosphatase Modulators written by José Luis Millán and published by Humana. This book was released on 2013-07-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one “how to” manual for basic scientists interested in initiating a drug discovery effort. While this book contains the traditional method chapters and some typical reviews on the structure and known functions of phosphatases, other contributions are meant to discuss approaches and alternatives useful in making “go/no-go” decisions in high throughput screening (HTS) and lead optimization campaigns. Many chapters focus on tissue-nonspecific alkaline phosphatase (TNAP) as well as protein phosphatases. Written for the highly successful Methods in Molecular Biology series, chapters in this volume include the kind of detail and key implementation advice that promotes reproducible results. Step-by-step and practical, Phosphatase Modulators offers a path to understanding many of the facets and complexities associated with undertaking a drug discovery effort and will serve as a roadmap to initiating those efforts.
Book Synopsis Microbial Biochemistry by : G. N. Cohen
Download or read book Microbial Biochemistry written by G. N. Cohen and published by Springer. This book was released on 2014-07-21 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial physiology, biochemistry and genetics allowed the formulation of concepts that turned out to be important in the study of higher organisms. In the first section, the principles of bacterial growth are given, as well as the description of the different layers that enclose the bacterial cytoplasm, and their role in obtaining nutrients from the outside media through different permeability mechanism described in detail. A chapter is devoted to allostery and is indispensable for the comprehension of many regulatory mechanisms described throughout the book. Another section analyses the mechanisms by which cells obtain the energy necessary for their growth, glycolysis, the pentose phosphate pathway, the tricarboxylic and the anaplerotic cycles. Two chapters are devoted to classes of microorganisms rarely dealt with in textbooks, namely the Archaea, mainly the methanogenic bacteria, and the methylotrophs. Eight chapters describe the principles of the regulations at the transcriptional level, with the necessary knowledge of the machineries of transcription and translation. The next fifteen chapters deal with the biosynthesis of the cell building blocks, amino acids, purine and pyrimidine nucleotides and deoxynucleotides, water-soluble vitamins and coenzymes, isoprene and tetrapyrrole derivatives and vitamin B12. The two last chapters are devoted to the study of protein-DNA interactions and to the evolution of biosynthetic pathways. The considerable advances made in the last thirty years in the field by the introduction of gene cloning and sequencing and by the exponential development of physical methods such as X-ray crystallography or nuclear magnetic resonance have helped presenting metabolism under a multidisciplinary attractive angle.
Book Synopsis Handbook of Toxicologic Pathology by : Wanda M. Haschek
Download or read book Handbook of Toxicologic Pathology written by Wanda M. Haschek and published by San Diego : Academic Press. This book was released on 2002 with total page 1012 pages. Available in PDF, EPUB and Kindle. Book excerpt: In volume I, the book covers toxicologic pathology in its basic aspects, including its definition, the basic biochemical and morphologic mechanisms underlying the discipline, the basic practice of toxicologic pathology (including special techniques) and issues essential to the understanding of toxicologic pathology such as risk assessment, experimental design, and statistical analysis. Next, the book moves to specific issues affecting the "practice" toxicologic pathology, including issues such as knowledge management, regulatory affairs and writing pathology reports. Finally, Volume I closes with several chapters that deal with specific classes of environmental toxicants such as endocrine disruptors and heavy metals. Volume II addresses the toxicologic pathology in a thoroughly standardized systems manner, addressing the basic structure and function of a particular organ system, its response to toxic injury, mechanisms of injury and methods of evaluation of such injury.-
Book Synopsis Protein Phosphatase Protocols by : John W. Ludlow
Download or read book Protein Phosphatase Protocols written by John W. Ludlow and published by Humana. This book was released on 2013-08-14 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: A major mechanism by which cells regulate protein function is to place phosphate groups on serine and threonine residues. Though the steady-state level of protein phosphorylation depends on the relative activities of both kinases and phosphatases, a much greater effort has previously gone into the study of the former that the latter . Today, however, there is an increasing appreciation for the role that protein phosphatases play in the dynamic p- cess of protein phosphorylation . To date, there are four major types of protein serine/threonine phosphatase catalytic subunits, designated protein phosphatase type 1, 2A, 2B, and 2C . Each has been identified by the techniques of protein chemistry and enzymology and can be distinguished from one another by their preference for specific substrates as well as their sensitivity to certain acti- tors and inhibitors . Protein Phosphatase Protocols has been assembled in response to the growing interest these enzymes are receiving . The goal of this compilation is to provide a "how-to" experimental guide to aid newcomers as well as s- soned veterans in their research endeavors, thus further contributing towards our ever increasing knowledge of serine/threonine phosphatases . What you have before you contains contributions by many of the current and emerging leaders in the field . To highlight just a few, these chapters c- tain step-by-step information on how to isolate novel phosphatases and re- latory subunits, assay for activity, and generate immunological reagents for both biochemical and biological characterization of these enzymes .
Book Synopsis The Protein Protocols Handbook by : John M. Walker
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Book Synopsis Gel Electrophoresis of Proteins by : B. D. Hames
Download or read book Gel Electrophoresis of Proteins written by B. D. Hames and published by . This book was released on 1981 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis PCR in Bioanalysis by : Stephen J Meltzer
Download or read book PCR in Bioanalysis written by Stephen J Meltzer and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Book Synopsis Cell Signalling by : John T. Hancock
Download or read book Cell Signalling written by John T. Hancock and published by Oxford University Press. This book was released on 2017 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'Cell Signalling' presents a carefully structured introduction to this subject, introducing those conserved features which underlie many different extra-and intracellular signalling systems.
Book Synopsis Stress Response by : Stephen M. Keyse
Download or read book Stress Response written by Stephen M. Keyse and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The p- tocols are carefully described by authors who have both devised and succe- fully employed them, and they represent a mixture not only of well-established techniques, but also new technologies at the leading edge of research. The areas covered include the detection and assay of stress-induced damage, the acti- tion of signal transduction pathways, stress-inducible gene expression, and stress protein function. Although no volume of this size can be comprehensive and the topics covered reflect a personal choice, it is hoped that it will prove of subst- tial interest and use to a wide range of research workers in the field.
Book Synopsis Affinity Chromatography by : Pascal Bailon
Download or read book Affinity Chromatography written by Pascal Bailon and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Book Synopsis Receptor Binding Techniques by : Mary Keen
Download or read book Receptor Binding Techniques written by Mary Keen and published by Springer Science & Business Media. This book was released on 1999 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This cutting-edge collection of step-by-step experimental protocols demonstrates
Book Synopsis Fluorescent Protein-Based Biosensors by : Jin Zhang
Download or read book Fluorescent Protein-Based Biosensors written by Jin Zhang and published by Humana. This book was released on 2013-09-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.
Book Synopsis Protein Gel Detection and Imaging by : Biji T. Kurien
Download or read book Protein Gel Detection and Imaging written by Biji T. Kurien and published by Humana. This book was released on 2019-08-24 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.
Book Synopsis Protein Tyrosine Phosphatases by : Damien Thévenin
Download or read book Protein Tyrosine Phosphatases written by Damien Thévenin and published by Springer Nature. This book was released on 2024-01-27 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. Furthermore, new advances in pharmacology and drug design have contributed to the developing novel therapeutics that target PTPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein Tyrosine Phosphatases: Methods and Protocols, Second Edition is a valuable resource for both experienced and novel researchers in this field, and will lead to discoveries and accelerated progress in the field of PTP, signal transduction, and drug development.
