A Practical Guide to Protein Engineering

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Author :
Publisher : Springer Nature
ISBN 13 : 3030568989
Total Pages : 213 pages
Book Rating : 4.0/5 (35 download)

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Book Synopsis A Practical Guide to Protein Engineering by : Tuck Seng Wong

Download or read book A Practical Guide to Protein Engineering written by Tuck Seng Wong and published by Springer Nature. This book was released on 2020-10-29 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

Protein engineering handbook. 2

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Author :
Publisher :
ISBN 13 :
Total Pages : 407 pages
Book Rating : 4.:/5 (316 download)

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Book Synopsis Protein engineering handbook. 2 by : Stefan H. Lutz

Download or read book Protein engineering handbook. 2 written by Stefan H. Lutz and published by . This book was released on 2009 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Engineering

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527344705
Total Pages : 41 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Protein Engineering by : Huimin Zhao

Download or read book Protein Engineering written by Huimin Zhao and published by John Wiley & Sons. This book was released on 2021-08-23 with total page 41 pages. Available in PDF, EPUB and Kindle. Book excerpt: A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.

Protein Engineering Techniques

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Author :
Publisher : Springer
ISBN 13 : 9811027323
Total Pages : 144 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Protein Engineering Techniques by : Krishna Mohan Poluri

Download or read book Protein Engineering Techniques written by Krishna Mohan Poluri and published by Springer. This book was released on 2016-11-12 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.

Protein Engineering Handbook, Volume 3

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527666982
Total Pages : 723 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Protein Engineering Handbook, Volume 3 by : Stefan Lutz

Download or read book Protein Engineering Handbook, Volume 3 written by Stefan Lutz and published by John Wiley & Sons. This book was released on 2012-09-14 with total page 723 pages. Available in PDF, EPUB and Kindle. Book excerpt: This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic. Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resulting in a superb overview of this burgeoning field.

Protein Engineering and Design

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Author :
Publisher : CRC Press
ISBN 13 : 1420076590
Total Pages : 434 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Protein Engineering and Design by : Sheldon J. Park

Download or read book Protein Engineering and Design written by Sheldon J. Park and published by CRC Press. This book was released on 2009-09-25 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science

Medicinal Protein Engineering

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Author :
Publisher : CRC Press
ISBN 13 : 1420007300
Total Pages : 588 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Medicinal Protein Engineering by : Yury E. Khudyakov

Download or read book Medicinal Protein Engineering written by Yury E. Khudyakov and published by CRC Press. This book was released on 2008-12-01 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: An All-Inclusive Review of the Achievements and Trends in the Fast-Growing Protein Engineering Field From humble beginnings like making fire for mere survival, engineering now steadfastly penetrates all aspects of our lives and even life itself at the molecular level. Protein engineering is a molecular biological discipline focused on designing and

Protein Engineering Protocols

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1597451878
Total Pages : 318 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Protein Engineering Protocols by : Kristian Müller

Download or read book Protein Engineering Protocols written by Kristian Müller and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Protein Engineering

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Author :
Publisher : Humana Press
ISBN 13 : 9781493973644
Total Pages : 350 pages
Book Rating : 4.9/5 (736 download)

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Book Synopsis Protein Engineering by : Uwe T. Bornscheuer

Download or read book Protein Engineering written by Uwe T. Bornscheuer and published by Humana Press. This book was released on 2017-10-30 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.

Protein Engineering

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Author :
Publisher : BoD – Books on Demand
ISBN 13 : 9535111388
Total Pages : 198 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Protein Engineering by : Tomohisa Ogawa

Download or read book Protein Engineering written by Tomohisa Ogawa and published by BoD – Books on Demand. This book was released on 2013-05-29 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Given the centrality of protein to many biological process, this book makes a significant contribution to the fields of healthcare and nutrition. Its chapters consider topics such as protein-protein and protein-ligand docking, and the protein engineering of enzymes involved in bioplastic metabolism. One contribution gives an overview of the In Vitro Virus (IVV) analytic method, while another shows how cutting-edge techniques in protein engineering advance our knowledge in the field of palaeontology. The book also includes a review of classic and alternative strategies when using yeasts in research, with a focus on Pichia pastoris as a host. Finally, there are two contributions on chromatography: one on the method itself, and another on its use to identify HMGB1-binding components.

Fermentation and Biochemical Engineering Handbook

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Author :
Publisher : William Andrew
ISBN 13 : 0815517130
Total Pages : 831 pages
Book Rating : 4.8/5 (155 download)

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Book Synopsis Fermentation and Biochemical Engineering Handbook by : Henry C. Vogel

Download or read book Fermentation and Biochemical Engineering Handbook written by Henry C. Vogel and published by William Andrew. This book was released on 1996-12-31 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a well-rounded handbook of fermentation and biochemical engineering presenting techniques for the commercial production of chemicals and pharmaceuticals via fermentation. Emphasis is given to unit operations fermentation, separation, purification, and recovery. Principles, process design, and equipment are detailed. Environment aspects are covered. The practical aspects of development, design, and operation are stressed. Theory is included to provide the necessary insight for a particular operation. Problems addressed are the collection of pilot data, choice of scale-up parameters, selection of the right piece of equipment, pinpointing of likely trouble spots, and methods of troubleshooting. The text, written from a practical and operating viewpoint, will assist development, design, engineering and production personnel in the fermentation industry. Contributors were selected based on their industrial background and orientation. The book is illustrated with numerous figures, photographs and schematic diagrams.

Protein Engineering Handbook

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527667016
Total Pages : 1021 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Protein Engineering Handbook by : Stefan Lutz

Download or read book Protein Engineering Handbook written by Stefan Lutz and published by John Wiley & Sons. This book was released on 2012-07-31 with total page 1021 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.

Engineering the Genetic Code

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Publisher : John Wiley & Sons
ISBN 13 : 3527607099
Total Pages : 312 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Engineering the Genetic Code by : Nediljko Budisa

Download or read book Engineering the Genetic Code written by Nediljko Budisa and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.

Handbook of Protein Engineering

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Publisher :
ISBN 13 : 9781632394101
Total Pages : 0 pages
Book Rating : 4.3/5 (941 download)

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Book Synopsis Handbook of Protein Engineering by : Anton Torres

Download or read book Handbook of Protein Engineering written by Anton Torres and published by . This book was released on 2015-02-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of protein engineering has been comprehensively illustrated in this book. The aim of this book is to provide state-of-the-art information regarding the field of protein engineering and elucidate its applications as well as technology. It covers a broad spectrum of significant topics like chromatography methodology, protein-protein and protein-ligand docking, protein engineering of enzymes involved in bio-plastic metabolism, etc. The book will appeal to a wide range of readers including researchers, scientists, and even students who wish to gain knowledge about the principles and practices of protein engineering.

Protein Engineering

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Author :
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 558 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Protein Engineering by : Jeffrey L. Cleland

Download or read book Protein Engineering written by Jeffrey L. Cleland and published by Wiley-Liss. This book was released on 1996-02-07 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: This systematic approach to protein engineering provides a thorough introduction to protein biophysics via chapters dealing with the experimental and theoretical determination of protein structure. Separate chapters discuss each of the expression systems c

Handbook of Food Proteins

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Author :
Publisher : Elsevier
ISBN 13 : 0857093630
Total Pages : 457 pages
Book Rating : 4.8/5 (57 download)

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Book Synopsis Handbook of Food Proteins by : Glyn O. Phillips

Download or read book Handbook of Food Proteins written by Glyn O. Phillips and published by Elsevier. This book was released on 2011-09-09 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: Traditionally a source of nutrition, proteins are also added to foods for their ability to form gels and stabilise emulsions, among other properties. The range of specialised protein ingredients used in foods is increasing. Handbook of food proteins provides an authoritative overview of the characteristics, functionalities and applications of different proteins of importance to the food industry in one convenient volume. The introductory chapter provides an overview of proteins and their uses in foods. The following chapters each focus on a particular protein ingredient or group of ingredients covering their origins, production, properties and applications. The proteins discussed are caseins, whey proteins, gelatin and other meat-derived protein ingredients, seafood proteins, egg proteins, soy proteins, pea and other legume proteins, mycoprotein, wheat gluten, canola and other oilseed proteins, algal proteins and potato protein. A chapter on texturised vegetable proteins completes the volume. Innovative products and potential methods for improving nutrition and diet using these proteins are described. With its distinguished editors and international team of expert contributors Handbook of food proteins is an invaluable reference tool for professionals using food protein ingredients for both food and other applications. An authoritative overview of the characteristics, functionalities and applications of different proteins of importance to the food industry Chapters each focus on a particular protein ingredient or group of ingredients Innovative products and potential methods for improving nutrition and diet using proteins is also described

The Protein Protocols Handbook

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Author :
Publisher : Springer
ISBN 13 : 1592591698
Total Pages : 1099 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis The Protein Protocols Handbook by : John M. Walker

Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.