Author : Divya Srinivasan
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (119 download)
Book Synopsis Profiling the Protein Corona of Human Saliva Interacted Dietary Particles and Understanding the Effect of Silica Particles on the Structure and Function of Salivary Alpha-amylase by : Divya Srinivasan
Download or read book Profiling the Protein Corona of Human Saliva Interacted Dietary Particles and Understanding the Effect of Silica Particles on the Structure and Function of Salivary Alpha-amylase written by Divya Srinivasan and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "While NPs (NPs) are copiously incorporated into different food products, the protein corona profile of saliva interacted dietary NPs and its potential influence on the function of proteins involved have received little attention in the past. In this study the most widely used NPs such as food grade silicon dioxide (SiO2), titanium dioxide (TiO2) and emerging NP with application in food industry- silver (Ag) were interacted with human saliva. These particles were characterized for their physicochemical properties in their pristine state and after interacting with saliva. Analysis of the protein corona composition of saliva interacted dietary particles were carried by employing 1D gel electrophoresis and LC-MS/MS. We observed a net decrease in the negative surface charge and change in agglomeration size in saliva interacted NPs. Significant differences in corona composition, and protein abundance were noted among the tested particles. Several proteins with vital functions in digestion and host protections were found to be enriched on the surface of particles. For instance, Lysozyme, Lactoferrin, Apolipoprotein B100 & E, and Annexin A2 were the most enriched proteins on NPs of SiO2. In order to verify if the binding of proteins have any effect on their function, we tested the amylase and lysozyme activities of saliva in the presence and absence of NPs of SiO2 and TiO2. Our results showed that these particles could compromise the enzymes function wherein NPs of SiO2 showed the highest effect. Overall, our study, identified salivary proteins involved in the protein corona of dietary particles and showed that vital functions of saliva such as digestion and antimicrobial properties could be partially affected by NP-Protein interactions in saliva"--