Preparative Chromatography

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Preparative Chromatography Techniques

Author : K. Hostettmann
Publisher : Springer Science & Business Media
ISBN 13 : 3662036312
Total Pages : 259 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Preparative Chromatography Techniques by : K. Hostettmann

Download or read book Preparative Chromatography Techniques written by K. Hostettmann and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past few years, increasing attention has been paid to the search for bioactive compounds from natural sources. The success of plant-derived products such as paclitaxel (Taxol) in tumor therapy or artemisinin in the treatment of malaria has provided the impetus for the introduction of numerous research programmes, especially in Industry. A great deal of effort is being expended in the generation of novel lead molecules of vegetable, marine and microbial origin by the use of high throughput screening protocols. When interesting hits are found, it is essen tial to have methods available for the rapid isolation of target compounds. For this reason, both industry and academia need efficient preparative chromatographic separation techniques and experience in their application. Purified natural products are required for complete spectro scopic identification and full characterization of new compo unds, for biological testing and for the supply of pharmaceuti cals, standards, and starting materials for synthetic work. Obtaining pure products from an extract can be a very long, te dious and expensive undertaking, involving many steps. Sometimes only minute amounts of the desired compounds are at hand and these entities may be labile. Thus it is an advantage to have access to as many different methods as possible in order to aid the isolation process. Although a certain amount of trial and error may be involved, nowadays there is the possibility of devising suitable rapid separation schemes by a judicious choice of the different techniques available.

Preparative Chromatography

Author : H. Schmidt-Traub
Publisher : John Wiley & Sons
ISBN 13 : 3527649301
Total Pages : 545 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Preparative Chromatography by : H. Schmidt-Traub

Download or read book Preparative Chromatography written by H. Schmidt-Traub and published by John Wiley & Sons. This book was released on 2012-09-27 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and substantially extended to reflect the developments in this fast-changing field. It retains the interdisciplinary approach that elegantly combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. It also covers recent advances in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. The increase in biopharmaceutical substances is reflected by new and revised chapters on different modifications of continuous chromatography as well as ion-exchange chromatography and other separation principles widely used in biochromatography. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on stationary phases, selection of chromatographic systems and process concepts. A completely new chapter deals with engineering and operation of chromatographic equipment. Final chapters on modeling and determination of model parameters as well as model based design, optimization and control of preparative chromatographic processes allow for optimal selection of chromatographic processes. Essential for chemists and chemical engineers in the chemical, pharmaceutical, and food industries.

Fundamentals of Preparative and Nonlinear Chromatography

Author : Georges Guiochon
Publisher : Academic Press
ISBN 13 : 0123705371
Total Pages : 991 pages
Book Rating : 4.1/5 (237 download)

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Book Synopsis Fundamentals of Preparative and Nonlinear Chromatography by : Georges Guiochon

Download or read book Fundamentals of Preparative and Nonlinear Chromatography written by Georges Guiochon and published by Academic Press. This book was released on 2006-03-21 with total page 991 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Fundamentals of Preparative and Nonlinear Chromatography is devoted to the fundamentals of a new process of purification or extraction of chemicals or proteins widely used in the pharmaceutical industry and in preparative chromatography. This process permits the preparation of extremely pure compounds satisfying the requests of the US Food and Drug Administration. The book describes the fundamentals of thermodynamics, mass transfer kinetics, and flow through porous media that are relevant to chromatography. It presents the models used in chromatography and their solutions, discusses the applications made, describes the different processes used, their numerous applications, and the methods of optimization of the experimental conditions of this process.

Preparative Chromatography for Separation of Proteins

Author : Arne Staby
Publisher : John Wiley & Sons
ISBN 13 : 1119031176
Total Pages : 608 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Preparative Chromatography for Separation of Proteins by : Arne Staby

Download or read book Preparative Chromatography for Separation of Proteins written by Arne Staby and published by John Wiley & Sons. This book was released on 2017-02-02 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers

Preparative and Production Scale Chromatography

Author : G. Ganetsos
Publisher : CRC Press
ISBN 13 : 9780824787387
Total Pages : 812 pages
Book Rating : 4.7/5 (873 download)

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Book Synopsis Preparative and Production Scale Chromatography by : G. Ganetsos

Download or read book Preparative and Production Scale Chromatography written by G. Ganetsos and published by CRC Press. This book was released on 1992-09-16 with total page 812 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines.

Preparative Chromatography Techniques

Author : Kurt Hostettmann
Publisher : Springer Science & Business Media
ISBN 13 : 3662024926
Total Pages : 146 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Preparative Chromatography Techniques by : Kurt Hostettmann

Download or read book Preparative Chromatography Techniques written by Kurt Hostettmann and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last few years several new instrumental techniques have been introduced for chromatographie separations. In addition, rapid developments in existing methods, such as preparative HPLC, have taken place. Despite these advances, how ever, a handbook covering the various preparative aspects ofthe new separation tech niques does not exist. This book is an attempt to fill the gap and to present a compila tion of modern separation techniques that will be useful for researchers faced with day-to-day preparative problems. Numerous examples of separations have been selected in order to show the possibilities (and also the limits) of each technique treated. These are often either applications from our own laboratory or else they reflect the approach we have been following for the isolation of natural products from plant sourees. Owing to the large number of published papers and the diversity of secondary plant constituents. an exhaustive survey of the literature has not been undertaken. We hope, however, that the examples selected will suggest to the reader which technique(s) and which conditions to choose for a particular isolation problem in the field of natural products. For invaluable help in the preparation of the manuscript for this book, we would like to thank Corinne Appolonia and Christine Marston. Lausanne, November 1985 K. Hostettmann M. Hostettmann A . . Marston Foreword Although not many people realize this, isolation and purification of biologically active materials is becoming increasingly crucial.

A Practical Handbook of Preparative HPLC

Author : Donald A Wellings
Publisher : Elsevier
ISBN 13 : 0080458858
Total Pages : 193 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis A Practical Handbook of Preparative HPLC by : Donald A Wellings

Download or read book A Practical Handbook of Preparative HPLC written by Donald A Wellings and published by Elsevier. This book was released on 2011-04-18 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...•Provides practical, hands-on advice based on years of experience•Will help ensure optimal design, equipment and separation results for your particular task•Presents system layouts from laboratory to process scale•Will help you to devise or improve record-keeping and documentation systems·Provides practical, hands-on advice based on years of experience·Will help ensure optimal design, equipment and separation results for your particular task·Presents system layouts from laboratory to process scale·Will help you to devise or improve record-keeping and documentation systems

Preparative Liquid Chromatography

Author : B.A. Bidlingmeyer
Publisher : Elsevier
ISBN 13 : 0080858414
Total Pages : 357 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Preparative Liquid Chromatography by : B.A. Bidlingmeyer

Download or read book Preparative Liquid Chromatography written by B.A. Bidlingmeyer and published by Elsevier. This book was released on 1987-07-01 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus.The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc.

Preparative Chromatography

Author : H. Schmidt-Traub
Publisher : John Wiley & Sons
ISBN 13 : 3527605908
Total Pages : 485 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Preparative Chromatography by : H. Schmidt-Traub

Download or read book Preparative Chromatography written by H. Schmidt-Traub and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This interdisciplinary approach combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. The book covers recent developments in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on solid materials and packed columns process concepts. Final chapters on modeling and determination of model parameters, the design and optimization of preparative chromatographic processes and chromatographic reactors allow for the optimum selection of chromatographic systems. Essential for chemists and engineers working in the chemicals and pharmaceutical industries as well as for food technologies, due to the interdisciplinary nature of these processes.

Preparative Chromatography

Author : H. Schmidt-Traub
Publisher : John Wiley & Sons
ISBN 13 : 352781633X
Total Pages : 749 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Preparative Chromatography by : H. Schmidt-Traub

Download or read book Preparative Chromatography written by H. Schmidt-Traub and published by John Wiley & Sons. This book was released on 2020-02-27 with total page 749 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this popular work is revised to include the latest developments in this fast-changing field. Its interdisciplinary approach elegantly combines the chemistry and engineering to explore the fundamentals and optimization processes involved.

Scale-Up and Optimization in Preparative Chromatography

Author : Anurag Rathore
Publisher : CRC Press
ISBN 13 : 9780203909287
Total Pages : 368 pages
Book Rating : 4.9/5 (92 download)

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Book Synopsis Scale-Up and Optimization in Preparative Chromatography by : Anurag Rathore

Download or read book Scale-Up and Optimization in Preparative Chromatography written by Anurag Rathore and published by CRC Press. This book was released on 2002-09-26 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting guidelines to predict and improve separation system performance, this book contains numerous case studies illustrating the practice of scale-up principles in process development. It offers solutions to limitations that occur in real-world purification schemes; methods to model, optimize, and characterize nonlinear separation processes; d

Modeling for Preparative Chromatography

Author : Georges Guiochon
Publisher : Academic Press
ISBN 13 : 9780120449835
Total Pages : 354 pages
Book Rating : 4.4/5 (498 download)

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Book Synopsis Modeling for Preparative Chromatography by : Georges Guiochon

Download or read book Modeling for Preparative Chromatography written by Georges Guiochon and published by Academic Press. This book was released on 2003-05-19 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonlinear chromatography is a field that borders both chemical engineering and physical chemistry. In turn, the theory of nonlinear chromatography is the foundation of preparative chromatography, a separation process that has lately become of considerable interest in the pharmaceutical industry. Only chromatography is sufficiently flexible and powerful to satisfy the practical requirements encountered in most difficult separations of pharmaceuticals and pharmaceutical intermediates. Since "nonlinear" behaviour is strictly a mathematical concept, it is difficult to leave mathematics out of any fundamental study of nonlinear chromatography. Therefore, Modeling for Preparative Chromatography will describe the different mathematical models of chromatography, examine the assumptions on which they are based, consider their properties, and discuss their solutions. All this will be done from a mathematical analysis point of view, paying considerable attention to the influence of nonlinear behavior on the results. Clear and simple discussions of the basic physico-chemical phenomena involved in HPLC Clear and complete presentation of the relevant properties of the mathematical tools involved Detailed analysis of nonlinear effects

Preparative Layer Chromatography

Author : Teresa Kowalska
Publisher : CRC Press
ISBN 13 : 1420005820
Total Pages : 441 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Preparative Layer Chromatography by : Teresa Kowalska

Download or read book Preparative Layer Chromatography written by Teresa Kowalska and published by CRC Press. This book was released on 2006-02-03 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compoun

Ion-Exchange Sorption and Preparative Chromatography of Biologically Active Molecules

Author : G.V. Samsonov
Publisher : Springer Science & Business Media
ISBN 13 : 1468489089
Total Pages : 175 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Ion-Exchange Sorption and Preparative Chromatography of Biologically Active Molecules by : G.V. Samsonov

Download or read book Ion-Exchange Sorption and Preparative Chromatography of Biologically Active Molecules written by G.V. Samsonov and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the physico-chemical principles underlying ion exchange sorption and chromatog~aphy. It does not cover in any detail the experimental and instrumental aspects of practical separations. The author has developed the subject starting from the synthesis and structure of the ion exchangers employed, through the thermodynamics of sorption selectivity and the equilibrium dynamics of ion sorption, to the kinetics and dynamics of non-equilibrium ion-exchange systems. Throughout this treatment the additional factors arising from the exchange of complex organic ions, as opposed to simple mineral ones, have been interwoven. The author has stressed the application in the separation of organic ions with biological activity, many of which are synthesized in biotechnological processes, and in view of this he uses the expression "physico-chemical biotechnology." In practice, however, his in-depth treatment is applicable to any charged organic species with multifunction ality and/or high molecular weight, and is therefore by no means restricted to biologically active materials, and certainly not to those molecules from a biotechnological source. Bearing this in mind, the text has a much wider value than the title may convey.

Preparative Scale Chromatography

Author : Eli Grushka
Publisher : CRC Press
ISBN 13 : 1000447308
Total Pages : 0 pages
Book Rating : 4.0/5 (4 download)

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Book Synopsis Preparative Scale Chromatography by : Eli Grushka

Download or read book Preparative Scale Chromatography written by Eli Grushka and published by CRC Press. This book was released on 2021-08-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses a model for the propagation of a finite concentration zone in a chromatographic column for the case of a single component sample, and explores operating conditions of ion-exchange chromatography for the production of albumin from bovine serum and plasma. Focus is on theoretical, instrument

Introduction to Modern Liquid Chromatography

Author : Lloyd R. Snyder
Publisher : John Wiley & Sons
ISBN 13 : 1118210395
Total Pages : 831 pages
Book Rating : 4.1/5 (182 download)

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Book Synopsis Introduction to Modern Liquid Chromatography by : Lloyd R. Snyder

Download or read book Introduction to Modern Liquid Chromatography written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Practical HPLC Method Development

Author : Lloyd R. Snyder
Publisher : John Wiley & Sons
ISBN 13 : 1118591518
Total Pages : 665 pages
Book Rating : 4.1/5 (185 download)

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Book Synopsis Practical HPLC Method Development by : Lloyd R. Snyder

Download or read book Practical HPLC Method Development written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2012-12-03 with total page 665 pages. Available in PDF, EPUB and Kindle. Book excerpt: This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.