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Plant Pathology Techniques Protocols
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Download or read book Plant Pathology written by Robert Burns and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.
Book Synopsis Molecular Methods in Plant Disease Diagnostics by : Neil Boonham
Download or read book Molecular Methods in Plant Disease Diagnostics written by Neil Boonham and published by CABI. This book was released on 2016-04-19 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
Book Synopsis Plant Fungal Pathogens by : Melvin Dennis Bolton
Download or read book Plant Fungal Pathogens written by Melvin Dennis Bolton and published by Springer Science+Business Media. This book was released on 2012 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: Including reproducible laboratory protocols, this guide to fungal pathogens in plants has been written by expert researchers in the field and includes methods now used to study them, including techniques for model systems such as Arabidopsis thaliana.
Book Synopsis Practical Plant Virology by : Jeanne Dijkstra
Download or read book Practical Plant Virology written by Jeanne Dijkstra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viruses require a special approach to establish their presence in a diseased plant since they are not visible, even under a light microscope. This manual describes in detail a variety of protocols for determining the properties and identity of a virus and its behavior in infected plants. A Springer Lab Manual.
Book Synopsis Laboratory Protocols in Fungal Biology by : Vijai Kumar Gupta
Download or read book Laboratory Protocols in Fungal Biology written by Vijai Kumar Gupta and published by Springer Science & Business Media. This book was released on 2012-12-09 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology
Book Synopsis Fungal Plant Pathogens, 2nd Edition by : Charles R. Lane
Download or read book Fungal Plant Pathogens, 2nd Edition written by Charles R. Lane and published by CABI. This book was released on 2023-07-30 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: This substantially updated edition now in full colour provides key techniques used when working with fungal and fungal-like plant pathogens. As a practical manual it also deals with disease recognition, detection and identification of fungi, plus methods to characterise and curate fungi and handle them under quarantine and quality assurance systems. Fungal Plant Pathogens: Applied Techniques, 2nd edition provides a valuable guide to investigating fungal plant diseases and interpreting laboratory findings for postgraduate and advanced undergraduate students, extension plant pathologists, consultants and advisers in agriculture, forestry and horticulture, and the food supply chain.
Book Synopsis Plant Microtechniques and Protocols by : Edward Chee Tak Yeung
Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by Springer. This book was released on 2015-09-19 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Book Synopsis Testing Methods for Seed-transmitted Viruses by : S. E. Albrechtsen
Download or read book Testing Methods for Seed-transmitted Viruses written by S. E. Albrechtsen and published by CABI. This book was released on 2006 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.
Book Synopsis Plant Pathology by : Christophe Lacomme
Download or read book Plant Pathology written by Christophe Lacomme and published by Humana. This book was released on 2015-05-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories.
Book Synopsis An Introduction to the Diagnosis of Plant Disease by : Chelston W. D. Brathwaite
Download or read book An Introduction to the Diagnosis of Plant Disease written by Chelston W. D. Brathwaite and published by IICA. This book was released on 1981 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diagnosis of plant disease; The microscope; The autoclave; The preparation of media for fungal and bacterial growth; Detection of fungal pathogens in infected plant tissues; Detection of bacterial pathogens in infected tissues; Koch's postulates; The diagnosis of a nematode prblem; Viruses and plant virus diseases; Mycoplasma as agents of plant disease.
Book Synopsis Essential Plant Pathology by : Gail Lynn Schumann
Download or read book Essential Plant Pathology written by Gail Lynn Schumann and published by American Phytopathological Society. This book was released on 2010 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides an explanation of how plant diseases are diagnosed, the 'plant disease triangle', how to determine the cause of a specific disease, what 'biotrophs' and necrotrophs are, disease cycles and how they can be utilized. Specific chapters address plant diseases caused by fungi, bacteria, nematodes, viruses, parasitic flowering plants, abiotic factors of the environment including light, temperature, and atmospheric gases, pathogens, how people influence plant disease epidemics, the prevention or management of plant disease epidemics, and more.
Book Synopsis Phytopathogenic Bacteria and Plant Diseases by : BS Thind
Download or read book Phytopathogenic Bacteria and Plant Diseases written by BS Thind and published by CRC Press. This book was released on 2019-08-08 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of Phytobacteriology is rapidly advancing and changing, because of recent advances in genomics and molecular plant pathology, but also due to the global spread of bacterial plant diseases and the emergence of new bacterial diseases. So, there is a need to integrate understanding of bacterial taxonomy, genomics, and basic plant pathology that reflects state-of-the-art knowledge about plant-disease mechanisms. This book describes seventy specific bacterial plant diseases and presents up-to-date classification of plant pathogenic bacteria. It would be of great help for scientists and researchers in conducting research on ongoing projects or formulation of new research projects. The book will also serve as a text book for advanced undergraduate and postgraduate students of disciplines of Phytobacteriology and Plant Pathology. Contains latest and updated information of plant pathogenic bacteria till December 2018 Describes seventy specific bacterial diseases Presents classification of the bacteria and associated nomenclature based on Bergey’s Manual Systematic Bacteriology and International Journal of Systematic and Evolutionary Microbiology Discusses practical and thoroughly tested disease management strategies that would help in controlling enormous losses caused by these plant diseases Reviews role of Type I-VI secretion systems and peptide- or protein-containing toxins produced by bacterial plant pathogens Briefs about plants and plant products that act as carriers of human enteric bacterial pathogens, like emphasizing role of seed sprouts as a common vehicle in causing food-borne illness Dr B. S. Thind was ex-Professor-cum-Head, Department of Plant Pathology, Punjab Agricultural University Ludhiana, India. He has 34 years of experience in teaching, research, and transfer of technology. He has conducted research investigations on bacterial blight of rice, bacterial stalk rot of maize, bacterial blight of cowpea, bacterial leaf spot of green gram, bacterial leaf spot of chillies and bacterial soft rot of potatoes. He also acted as Principal Investigator of two ICAR-funded research schemes entitled, "Detection and control of phytopathogenic bacteria from cowpea and mungbean seeds from 1981 to 1986 and "Perpetuation, variability, and control of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight of rice" from 1989 to 1993, and also of a DST funded research scheme "Biological control of bacterial blight, sheath blight, sheath rot, and brown leaf spot of rice" from 1999 to 2002. He also authored a manual entitled, "Plant Bacteriology" and a text book entitled, "Phytopathogenic Procaryotes and Plant Diseases" published by Scientific Publishers (India). He is Life member of Indian Phytopathological Society, Indian Society of Plant Pathologists, Indian Society of Mycology and Plant Pathology, and Indian Science Congress Association.
Book Synopsis Laboratory Techniques in Plant Bacteriology by : Suresh G. Borkar
Download or read book Laboratory Techniques in Plant Bacteriology written by Suresh G. Borkar and published by CRC Press. This book was released on 2017-12-12 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Techniques in Plant Bacteriology is ideal for scientists and students who seek a career in plant pathogenic bacteria. This book contains 41 chapters comprising practicable techniques from isolation of bacterial plant pathogens to their identification up to species and race/biotype level. It includes identification protocols of morphological, biochemical, immunological, and molecular-based techniques. This book comprises all technological aspects of plant bacteriological studies. Its content is ideal for graduate students and research scholars including bacteriological professionals or technicians. The book ultimately provides working technologies useful for controlling bacterial disease pathogens.
Book Synopsis Basic Plant Pathology Methods by : James B. Sinclair
Download or read book Basic Plant Pathology Methods written by James B. Sinclair and published by CRC Press. This book was released on 1995-04-05 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following: Sterilization of culture apparatus and culture media Culture of pathogens with detailed techniques for 61 fungi and selected bacteria Long-term storage of plant pathogens Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens Establishment of disease and testing for disease resistance Work with soil microorganisms Fungicide evaluation Biological control Bright-field microscopy
Book Synopsis Manual of Techniques in Insect Pathology by : Lawrence A. Lacey
Download or read book Manual of Techniques in Insect Pathology written by Lawrence A. Lacey and published by Academic Press. This book was released on 1997-02-27 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.This book will be an essential laboratory reference for insect pathologists.Features include:* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens* Details of the practical knowledge needed by beginners to apply the techniques* Chapters written by an international group of experts* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques* Provides extensive supplemental literature and recipes for media, fixatives and stains
Book Synopsis Microbial Plant Pathogens-Detection and Disease Diagnosis: by : P. Narayanasamy
Download or read book Microbial Plant Pathogens-Detection and Disease Diagnosis: written by P. Narayanasamy and published by Springer Science & Business Media. This book was released on 2010-10-20 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.
Book Synopsis Introduction to Plant Pathology by : Richard N. Strange
Download or read book Introduction to Plant Pathology written by Richard N. Strange and published by John Wiley & Sons. This book was released on 2006-02-08 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: This invaluable resource introduces the eleven types of organism that cause plant disease, ranging from higher plants to viroids and describes examples of cash and staple crop diseases that have caused human catastrophes. Early chapters cover serological and molecular techniques for the diagnosis of plant pathogens, epidemiology, methods for estimating disease severity and its effect on crop yields and techniques for limiting inoculum. Later chapters are concerned with colonisation of the plant and symptom development and the underlying biochemical and genetic factors that control these events. Finally, the control of plant disease using a variety of techniques including genetic modification is discussed. Modern diagnostic techniques Epidemiology and the measurement of disease severity The biochemistry and molecular biology of plant disease Control through cultural, biological, genetic and molecular techniques A wealth of examples and applications including full colour photographs
