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Peptide And Protein Interaction With Membrane Systems
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Book Synopsis Peptide and Protein Interaction with Membrane Systems by : Sara Bobone
Download or read book Peptide and Protein Interaction with Membrane Systems written by Sara Bobone and published by Springer. This book was released on 2014-05-31 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: In her thesis, Sara Bobone outlines spectroscopic studies of antimicrobial peptides (AMPs) which are promising lead compounds for drugs used to fight multidrug resistant bacteria. Bobone shows that AMPs interact with liposomes and she clarifies the structure of pores formed by one of these molecules. These results help us to understand how AMPs are selective for bacterial membranes and how their activity can be finely tuned by modifying their sequence. Findings which solve several conundrums debated in the literature for years. In addition, Bobone uses liposomes as nanotemplates for the photopolymerization of hydrogels - exploiting the self- assembly properties of phospholipids. Bobone was able to trap an enzyme using nanometeric particles, while still allowing its activity by the diffusion of substrates and products through the network of the polymer. The innovative nano devices described in this thesis could solve many of the hurdles still hampering the therapeutic application of protein-based drugs.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Inhibitors of Protein–Protein Interactions by : Ali Tavassoli
Download or read book Inhibitors of Protein–Protein Interactions written by Ali Tavassoli and published by Royal Society of Chemistry. This book was released on 2020-12-07 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-protein interactions (PPI) are at the heart of the majority of cellular processes, and are frequently dysregulated or usurped in disease. Given this central role, the inhibition of PPIs has been of significant interest as a means of treating a wide variety of diseases. However, there are inherent challenges in developing molecules capable of disrupting the relatively featureless and large interfacial areas involved. Despite this, there have been a number of successes in this field in recent years using both traditional drug discovery approaches and innovative, interdisciplinary strategies using novel chemical scaffolds. This book comprehensively covers the various aspects of PPI inhibition, encompassing small molecules, peptidomimetics, cyclic peptides, stapled peptides and macrocycles. Illustrated throughout with successful case studies, this book provides a holistic, cutting-edge view of the subject area and is ideal for chemical biologists and medicinal chemists interested in developing PPI inhibitors.
Book Synopsis Peptide-Lipid Interactions by : Sidney A. Simon
Download or read book Peptide-Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
Book Synopsis Peptide Arrays on Membrane Supports by : Joachim Koch
Download or read book Peptide Arrays on Membrane Supports written by Joachim Koch and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.
Download or read book Peptide-membrane Interactions written by and published by . This book was released on 2021 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume will address several related aspects of peptide interactions with membranes. It will consider model theoretical and experimental systems in order to define the ‘reaction space’ that is possible and where appropriate with relevance to fundamental questions in cell biology, including how peptides and proteins behave within biological membranes. The topics covered include: Theoretical and experimental comparisons of simple peptide-membrane systems ; Theoretical and experimental studies of complex peptide-membrane systems ; Behaviour and interactions of proteins and peptides with and within membranes ; Peptide-membrane interactions and biotechnology.
Book Synopsis Using Molecular Dynamics Simulations and Statistical Modeling to Explore Membrane Structure and Membrane-protein Interactions by : Shushan He
Download or read book Using Molecular Dynamics Simulations and Statistical Modeling to Explore Membrane Structure and Membrane-protein Interactions written by Shushan He and published by . This book was released on 2018 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure of cellular membranes gives rise to important biological phenomena, and understanding the (de)mixing behavior of multicomponent lipid bilayers is an important step toward unraveling the nature of spatial composition heterogeneities in cellular membranes and their role in biological function. In this dissertation we focus on the spatial organization and compositional heterogeneity of model membrane systems and their interaction with small, biologically relevant peptides. I employed both coarse-grained and atomistic molecular dynamics simulations to study the composition phase diagram of a quaternary mixture of phospholipids and cholesterol, as well as to sample the configurational space of peptide-membrane interactions. I investigate the mechanisms controlling membrane spatial heterogeneity and membrane protein interaction. This work yields important new insight into both the structural properties of lipid bilayer systems with spatial and compositional heterogeneity at vastly different length scales, which has prompted numerous publications in the field seeking for a plausible mechanism. This work will also provide perspectives on configurations of the PAP248-286 peptide upon interacting with membranes, which, despite its importance for human health, has not received as much attention from the research community as other amyloid-forming peptides. In this wide-open field such simulations will have significant scientific impact.
Download or read book Peptide-Membrane Interactions written by and published by . This book was released on 2022-01-28 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume will address several related aspects of peptide interactions with membranes. It will consider model theoretical and experimental systems with relevance to fundamental questions in cell biology, including how peptides and proteins behave within biological membranes.
Book Synopsis Studies of Protein-protein Interactions at Membrane Interfaces Using Correlated Atomic Force and Fluorescence Microscopy by : James Eric Shaw
Download or read book Studies of Protein-protein Interactions at Membrane Interfaces Using Correlated Atomic Force and Fluorescence Microscopy written by James Eric Shaw and published by . This book was released on 2007 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Processes occurring at membrane interfaces are important for cellular and biological functions. The inability to readily crystallize membrane proteins for X-ray diffraction, or in solution NMR to study proteins with large molecular weight and to retain all intramolecular structures of the membrane proteins during their solubilisation process by detergents are the key reasons for a scarcity of atomic-resolution membrane protein structures. This has hindered our understanding of the structure-function relationship of membrane proteins. New strategies for studying membrane proteins will provide more insights. In this thesis, we have coupled atomic force microscopy and two fluorescence microscopy techniques---total internal reflectance fluorescence microscopy and confocal laser scanning microscopy, to visualize protein-membrane interactions and dynamics in model membrane systems. To validate our correlated approach, we investigated the partitioning of various lipid fluorescent probes in phase-separated supported planar bilayers. We applied the same methodology in two case studies of peptide-membrane interactions in supported planar bilayers: (1) indolicidin, a cationic 13 amino-acid peptide isolated from bovine neutrophils, as a model of antimicrobial peptide-membrane interactions, and (2) NAP-22 peptide, a cationic myristoylated peptide crucial for neuronal growth and plasticity, as a model of protein-induced lipid recruitment. Using this combined AFM-fluorescence approach with supported planar bilayers, we showed that: (1) the attachment of a fluorescent dye molecule can alter the physical properties of the host lipid and inhibit the lipid-dye conjugate from associating with raft domains, (2) indolicidin preferentially associates with the more disordered fluid domain and induces gel domain lowering in a concentration- and lipid-dependent manner, and (3) NAP-22 peptide induces coalescence of raft domains at low peptide concentration and phase-immersion at high peptide concentration resulting the co-localization of cholesterol and phosphatidylinositol biphosphates. This work demonstrates the versatility of a combined AFM-fluorescence approach as well as its limitations of the current configurations.
Book Synopsis Peptides Targeting Protein-Protein Interactions: Methods and Applications by : Luca Domenico D’Andrea
Download or read book Peptides Targeting Protein-Protein Interactions: Methods and Applications written by Luca Domenico D’Andrea and published by Frontiers Media SA. This book was released on 2021-12-06 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Biological Solid-State NMR by : Frances Separovic
Download or read book Advances in Biological Solid-State NMR written by Frances Separovic and published by Royal Society of Chemistry. This book was released on 2014 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biological NMR brings the reader up to date with chapters from international leaders of this growing field, covering the most recent developments in the methodology and applications of solid state NMR to studies of membrane interactions and molecular motions
Book Synopsis Protein-Lipid Interactions by : C.Reyes Mateo
Download or read book Protein-Lipid Interactions written by C.Reyes Mateo and published by Springer Science & Business Media. This book was released on 2006-01-31 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of protein–lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.
Book Synopsis Synthesis, Organization and Structural Studies of Peptide Motifs Interacting with Membrane Systems by : Philipp E. Schneggenburger
Download or read book Synthesis, Organization and Structural Studies of Peptide Motifs Interacting with Membrane Systems written by Philipp E. Schneggenburger and published by . This book was released on 2010 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins in biological membranes represent up to 30% of all proteins in sequenced genomes. While certain membrane proteins are functional as monomers others need to assemble into oligomeric structures to carry out their biochemical role. Due to the complexity and dynamics of these interactions, they are often hardly traceable and nearly impossible to predict. For this respect, peptide model systems provide a valuable tool in studying membrane associated processes, since they are synthetically accessible and can be readily modified. Herein, the design and synthesis of peptide/peptide nucleic acid (PNA) conjugates as well as their functional analyses in lipid complexes are reported. The respective species exhibit a high-affinity recognition in the membrane adjacent water layers prior to assembly of the transmembrane domains. Analytical studies of the novel model system include X-ray scattering and F rster resonance energy transfer assays.
Book Synopsis New Developments in Lipid-Protein Interactions and Receptor Function by : K.W.A. Wirtz
Download or read book New Developments in Lipid-Protein Interactions and Receptor Function written by K.W.A. Wirtz and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: A NATO Advanced Study Institute on "New Developments in Lipid-Protein Interactions and Receptor Function" was held on the Island of Spetsai, Greece, from August 16-27, 1992. This Institute was organized to bring together researchers in the field of membrane organization and dynamics with those actively involved in studies on receptor function, signal transduction mechanisms and gene regulation. 2 Presentations and discussions focussed on the regulation of intracellular Ca +-levels, on the second messengers derived from inositol lipids and on the specific phospholipase C isozymes involved in these processes. A major focus was on G-proteins and the effect of lipid anchors on their function. These principles of regulation were further discussed in the context of receptors for acetylcholine, lysophosphatidic acid and low-density lipoproteins. In addition, various aspects of the genomic regulation of cell growth and differentiation by transcription factors were presented. These topics were put into perspective by discussing the most recent developments in lipid-protein interactions, protein insertion into membranes, membrane lipid organization and lipid dynamics as mediated by phospholipid transfer proteins. This book presents the content of the major lectures and a selection of the most relevant of the most important topics posters. These proceedings offer a comprehensive account presented during the course of the Institute. The book is intended to make these proceedings accessible to a large audience.
Book Synopsis Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification by : Jianpeng Wang
Download or read book Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification written by Jianpeng Wang and published by Springer. This book was released on 2016-09-26 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis focuses on the study of interactions between protein and peptides and their potential applications in cell imaging and nanoparticle surface modification. Drawing inspiration from naturally occurring coiled-coil binding pairs, it proposes a novel covalent peptide tag and probe system, based on the concept of “affinity guided covalent conjugation.” This newly established methodology provides complementary resolution to protein labeling, imaging and trafficking. By systematically investigating the coordination interaction between protein and quantum dots using various engineered protein ligands, this thesis proposes a general rule for protein self-assembly on the surface of quantum dots and reports a revolutionized nanobelt protein in accordance with this rule. It is an extraordinary example of interdisciplinary research, providing answers to real-life biological problems from a chemistry perspective.
Book Synopsis Free Energy Calculations by : Christophe Chipot
Download or read book Free Energy Calculations written by Christophe Chipot and published by Springer Science & Business Media. This book was released on 2007-01-08 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free energy constitutes the most important thermodynamic quantity to understand how chemical species recognize each other, associate or react. Examples of problems in which knowledge of the underlying free energy behaviour is required, include conformational equilibria and molecular association, partitioning between immiscible liquids, receptor-drug interaction, protein-protein and protein-DNA association, and protein stability. This volume sets out to present a coherent and comprehensive account of the concepts that underlie different approaches devised for the determination of free energies. The reader will gain the necessary insight into the theoretical and computational foundations of the subject and will be presented with relevant applications from molecular-level modelling and simulations of chemical and biological systems. Both formally accurate and approximate methods are covered using both classical and quantum mechanical descriptions. A central theme of the book is that the wide variety of free energy calculation techniques available today can be understood as different implementations of a few basic principles. The book is aimed at a broad readership of graduate students and researchers having a background in chemistry, physics, engineering and physical biology.
Book Synopsis Membrane Protein Protocols by : Barry S. Selinsky
Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
