Pcr Methods In Foods

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PCR Methods in Foods

Author : John Maurer
Publisher : Springer Science & Business Media
ISBN 13 : 0387317023
Total Pages : 152 pages
Book Rating : 4.3/5 (873 download)

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Book Synopsis PCR Methods in Foods by : John Maurer

Download or read book PCR Methods in Foods written by John Maurer and published by Springer Science & Business Media. This book was released on 2006-11-22 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will introduce non-molecular biologists to diagnostic PCR-based te- nologies for the detection of pathogens in foods. By the conclusion of this book, the reader should be able to: 1) understand the principles behind PCR including real-time; 2) know the basics involved in the design, optimization, and imp- mentation of PCR in food microbiology lab setting; 3) interpret results; 4) know limitations and strengths of PCR; and 5) understand the basic principles behind a new fledgling technology, microarrays and its potential applications in food microbiology. This book will provide readers with the latest information on PCR and microarray based tests and their application towards the detection of bacterial, protozoal and viral pathogens in foods. Figures, charts, and tables will be used, where appropriate, to help illustrate concepts or provide the reader with useful information or resources as an important starting point in bringing molecular diagnostics into the food microbiology lab. This book is not designed to be a “cookbook”PCR manual with recipes and step-by-step instructions but rather serve as a primer or resource book for students, faculty, and other professionals interested in molecular biology and its integration into food safety. v Table of Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Chapter 1. PCR Basics Amanda Fairchild, M. S. , Margie D. Lee DVM, Ph. D. , and John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. The Mythology of PCR: A Warning to the Wise John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Chapter 3.

Modern Techniques for Food Authentication

Author : Da-Wen Sun
Publisher : Academic Press
ISBN 13 : 0128142650
Total Pages : 808 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Modern Techniques for Food Authentication by : Da-Wen Sun

Download or read book Modern Techniques for Food Authentication written by Da-Wen Sun and published by Academic Press. This book was released on 2018-07-25 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Techniques for Food Authentication, Second Edition presents a comprehensive review of the novel techniques available to authenticate food products, including various spectroscopic technologies, methods based on isotopic analysis and chromatography, and other techniques based on DNA, enzymatic analysis and electrophoresis. This new edition pinpoints research and development trends for those working in research, development and operations in the food industry, giving them readily accessible information on modern food authentication techniques to ensure a safe and authentic food supply. It will also serve as an essential reference source to undergraduate and postgraduate students, and for researchers in universities and research institutions. Presents emerging imaging techniques that have proven to be powerful, non-destructive tools for food authentication Includes applications of hyperspectral imaging to reflect the current trend of developments in food imaging technology for each topic area Provides pixel level visualization techniques needed for fast and effective food sample testing Contains two new chapters on Imaging Spectroscopic Techniques

Real-time PCR in Food Science

Author : David Rodríguez-Lázaro
Publisher : Caister Academic Press Limited
ISBN 13 : 9781908230157
Total Pages : 0 pages
Book Rating : 4.2/5 (31 download)

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Book Synopsis Real-time PCR in Food Science by : David Rodríguez-Lázaro

Download or read book Real-time PCR in Food Science written by David Rodríguez-Lázaro and published by Caister Academic Press Limited. This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial detection and control are vital aspects of food microbiology. Real-time PCR is one of the most significant advances in this area, providing rapid, reliable, and quantitative results. In recent years, real-time PCR has become increasingly important to the agricultural and food industries as a valuable alternative to traditional detection methods. The advantages of quantitative real-time PCR include speed, an excellent detection limit, selectivity, specificity, sensitivity, and the potential for automation. Written by experts in the field, this book is an indispensable manual for scientists in the food industry. The first section provides an introduction to real-time PCR, discusses the use of PCR diagnostics in food science, describes the principles and methods of sample preparation, and covers the verification and control of PCR procedures. The second section covers the use of real-time PCR to detect various pathogens including Salmonella, Listeria, E. coli, Campylobacter, Yersinia, Staphylococcus, Clostridium, viruses, and parasites. Also included is a chapter on the standardization of real-time PCR methods in food microbiology. In the final section, the book covers the use of real-time PCR for the analysis of genetically modified organisms, for food allergens, and for identification of animal or plant species. This will be an invaluable book for anyone involved in food microbiology or the detection of foodborne pathogens, and it is a recommended volume for all microbiology laboratories.

PCR

Author : Lucília Domingues
Publisher : Springer Nature
ISBN 13 : 1071633589
Total Pages : 255 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis PCR by : Lucília Domingues

Download or read book PCR written by Lucília Domingues and published by Springer Nature. This book was released on 2023-09-23 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Handbook of Food Allergen Detection and Control

Author : Simon Flanagan
Publisher : Elsevier
ISBN 13 : 1782420215
Total Pages : 453 pages
Book Rating : 4.7/5 (824 download)

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Book Synopsis Handbook of Food Allergen Detection and Control by : Simon Flanagan

Download or read book Handbook of Food Allergen Detection and Control written by Simon Flanagan and published by Elsevier. This book was released on 2014-09-25 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Allergens in food and their detection, management and elimination constitute a key issue for food manufacturers, especially in terms of safety. This book reviews current and emerging technologies for detecting and reducing allergens, as well as issues such as traceability, regulation and consumer attitudes. Following an introductory chapter by a distinguished expert, part one covers allergen management throughout the food chain. Part two details current and emerging methods of allergen detection in food, and part three covers methods for reducing and eliminating allergens in food. Finally, part four focuses on the control and detection of individual food allergens and the risks each one presents in food manufacture. Reviews current and emerging technologies for detecting and reducing allergens, as well as issues such as traceability, regulation and consumer attitudes Covers allergen management throughout the food chain and reviews current and emerging methods of allergen detection Examines methods for reducing and eliminating allergens in food and provides a detailed overview of the control and detection of individual food allergens

Bacteriological Analytical Manual

Author : United States. Food and Drug Administration. Division of Microbiology
Publisher :
ISBN 13 :
Total Pages : 180 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Bacteriological Analytical Manual by : United States. Food and Drug Administration. Division of Microbiology

Download or read book Bacteriological Analytical Manual written by United States. Food and Drug Administration. Division of Microbiology and published by . This book was released on 1969 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

PCR Detection of Microbial Pathogens

Author : Konrad Sachse
Publisher : Springer Science & Business Media
ISBN 13 : 1588290492
Total Pages : 667 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis PCR Detection of Microbial Pathogens by : Konrad Sachse

Download or read book PCR Detection of Microbial Pathogens written by Konrad Sachse and published by Springer Science & Business Media. This book was released on 2003 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.

Rapid Cycle Real-Time PCR — Methods and Applications

Author : U. Reischl
Publisher : Springer Science & Business Media
ISBN 13 : 3642483518
Total Pages : 253 pages
Book Rating : 4.6/5 (424 download)

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Book Synopsis Rapid Cycle Real-Time PCR — Methods and Applications by : U. Reischl

Download or read book Rapid Cycle Real-Time PCR — Methods and Applications written by U. Reischl and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

PCR: A Powerful Method in Food Safety Field

Author : Oana-Maria Boldura
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (115 download)

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Book Synopsis PCR: A Powerful Method in Food Safety Field by : Oana-Maria Boldura

Download or read book PCR: A Powerful Method in Food Safety Field written by Oana-Maria Boldura and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this chapter, application of the polymerase chain reaction (PCR) technique in food safety, considering all the branches of this concept, is presented. The area of interest contains important analysis for both human health and the identification of food adulteration. PCR techniques used for detection of genetically modified organisms (GMO) in different matrices, identification of different animal species in meat and dairy products, as well as the detection of food infection with food-borne pathogens and toxicogenic fungi are described. The working methods and result analysis are exemplified, starting with DNA isolation adjusted to different matrices, detection of target genes, and validation for all of these methods. Techniques of simplex PCR, primer multiplexing, primer design, validation of the laboratory methods, optimization of the PCR results, and result interpretation through the analysis of the electrophoresis gels and sequencing data are studied. At the same time, the obtained results, the obstacles encountered, and how they were overcome could be an example for specific analysis developed with less resources and also for adapting the existent validated methods to the new laboratory conditions. The practical applicability and the consumer's demands are of great importance and always must be considered in developing and validating those methods.

Microbiology of Food and Animal Feeding Stuffs. Polymerase Chain Reaction (PCR) for the Detection of Food-Borne Pathogens. Requirements for Amplification and Detection for Qualitative Methods

Download Microbiology of Food and Animal Feeding Stuffs. Polymerase Chain Reaction (PCR) for the Detection of Food-Borne Pathogens. Requirements for Amplification and Detection for Qualitative Methods PDF Online Free

Author : British Standards Institute Staff
Publisher :
ISBN 13 : 9780580482397
Total Pages : 18 pages
Book Rating : 4.4/5 (823 download)

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Book Synopsis Microbiology of Food and Animal Feeding Stuffs. Polymerase Chain Reaction (PCR) for the Detection of Food-Borne Pathogens. Requirements for Amplification and Detection for Qualitative Methods by : British Standards Institute Staff

Download or read book Microbiology of Food and Animal Feeding Stuffs. Polymerase Chain Reaction (PCR) for the Detection of Food-Borne Pathogens. Requirements for Amplification and Detection for Qualitative Methods written by British Standards Institute Staff and published by . This book was released on 2006-04-28 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food products, Animal feed, Microbiology, Microbiological analysis, Pathogens, Microorganisms, Food testing, Biochemical methods of analysis, Enzymes, Nucleic acids, Deoxyribonucleic acid

PCR Protocols

Author : Michael A. Innis
Publisher : Academic Press
ISBN 13 : 008088671X
Total Pages : 501 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis PCR Protocols by : Michael A. Innis

Download or read book PCR Protocols written by Michael A. Innis and published by Academic Press. This book was released on 2012-12-02 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications

Polymerase Chain Reaction for Biomedical Applications

Author : Ali Samadikuchaksaraei
Publisher : BoD – Books on Demand
ISBN 13 : 9535127950
Total Pages : 186 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Polymerase Chain Reaction for Biomedical Applications by : Ali Samadikuchaksaraei

Download or read book Polymerase Chain Reaction for Biomedical Applications written by Ali Samadikuchaksaraei and published by BoD – Books on Demand. This book was released on 2016-12-14 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.

Molecular Techniques in Food Biology

Author : Aly Farag El Sheikha
Publisher : John Wiley & Sons
ISBN 13 : 111937460X
Total Pages : 456 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis Molecular Techniques in Food Biology by : Aly Farag El Sheikha

Download or read book Molecular Techniques in Food Biology written by Aly Farag El Sheikha and published by John Wiley & Sons. This book was released on 2018-04-09 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in agricultural, food science, biomedicine, and government involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.

Advances in microbial food safety

Author : K. Rantsiou
Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128089687
Total Pages : 16 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Advances in microbial food safety by : K. Rantsiou

Download or read book Advances in microbial food safety written by K. Rantsiou and published by Elsevier Inc. Chapters. This book was released on 2013-07-31 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the invention of the polymerase chain reaction (PCR) in the 1980s, applied molecular biology has progressed enormously. Following technological advancements, PCR analysis has become quantitative rather than qualitative. Quantitative PCR (qPCR) revolutionized molecular approaches to food safety, due to the ability to quantify specific pathogens in food. New methods for studying the transcription process, such as microarrays, can be used to investigate the behavior of pathogens in the food environment. Indeed, the effects of food–related conditions on gene expression in pathogenic microorganisms have already been demonstrated.

Rapid Cycle Real-Time PCR — Methods and Applications

Author : Udo Reischl
Publisher : Springer
ISBN 13 : 9783642483530
Total Pages : 258 pages
Book Rating : 4.4/5 (835 download)

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Book Synopsis Rapid Cycle Real-Time PCR — Methods and Applications by : Udo Reischl

Download or read book Rapid Cycle Real-Time PCR — Methods and Applications written by Udo Reischl and published by Springer. This book was released on 2012-12-12 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Microbiology Laboratory Guidebook

Author : United States. Food Safety and Inspection Service. Microbiology Division
Publisher :
ISBN 13 :
Total Pages : 634 pages
Book Rating : 4.3/5 ( download)

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Book Synopsis Microbiology Laboratory Guidebook by : United States. Food Safety and Inspection Service. Microbiology Division

Download or read book Microbiology Laboratory Guidebook written by United States. Food Safety and Inspection Service. Microbiology Division and published by . This book was released on 1998 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Biological and Immunological Techniques and Applications for Food Chemists

Author : Bert Popping
Publisher : John Wiley & Sons
ISBN 13 : 9780470524978
Total Pages : 464 pages
Book Rating : 4.5/5 (249 download)

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Book Synopsis Molecular Biological and Immunological Techniques and Applications for Food Chemists by : Bert Popping

Download or read book Molecular Biological and Immunological Techniques and Applications for Food Chemists written by Bert Popping and published by John Wiley & Sons. This book was released on 2010-03-16 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to using molecular biology and immunological methods for the analysis of food Many of the analytical problems that food chemists face in the lab cannot be solved by chemistry alone, and so analytical chemists are turning to molecular biology and immunology for alternative approaches. Molecular Biological and Immunological Techniques and Applications for Food Chemists comprehensively explains the most important molecular biology and immunology methods, and illustrates their application in food analysis. Written by a distinguished group of experts, the coverage includes: Molecular Biological Methods—techniques explained, laboratory layout, PCR, real-time PCR, RFLP, SSCP, and sequencing Molecular Biology Applications—meat, genetically modified organisms (GMOs), food allergens, offal, and fish Immunological Methods—techniques explained and antibody-based detection methods Immunology Applications—animal speciation, international food allergen regulations (except Japanese), Japanese regulations and buckwheat allergen detection, egg allergen detection, soy allergen detection, milk allergen detection, gluten allergen detection, nut allergen detection, fish allergen detection, lupin allergen detection, mustard allergen detection, and celery allergen detection Clearly written and consistently edited to provide information to a wide range of readers, Molecular Biological and Immunological Techniques and Applications for Food Chemists offers an up-to-date reference for food scientists in government and industry, policymakers, and graduate-level students of food science, technology, and engineering. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.