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Pcr Methods Express
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Book Synopsis PCR: Methods Express by : Simon Hughes
Download or read book PCR: Methods Express written by Simon Hughes and published by Scion Publishing Ltd. This book was released on 2007-05-01 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: *real time qRT-PCR *rapid generation of gene targeting constructs *PCR multiplexes *PCR-based mutagenesis *identification of microdeletions and microduplications *DNA methylation analysis *forensic genetic DNA typing *genotyping *identification of mutations in single cells *whole genome amplification *diagnosis of infectious diseases *inverse PCR-based RFLP This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.
Download or read book PCR written by Simon Hughes and published by Methods Express (Hardcover). This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "PCR: Methods Express" describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems.
Download or read book PCR written by Lucília Domingues and published by Springer Nature. This book was released on 2023-09-23 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Book Synopsis Gene Quantification by : Francois Ferre
Download or read book Gene Quantification written by Francois Ferre and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Download or read book Poisoning written by Ntambwe Malangu and published by BoD – Books on Demand. This book was released on 2017-12-20 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, which is the result of contributions from a team of international authors, presents a collection of materials that can be categorized into two groups. The first group of papers deals with clinical toxicology topics including poisoning by anticoagulant rodenticides, food toxins, carbon monoxide, the toxicity of beta-lactam antibiotics, acute neonicotinoid poisoning, occupational risk factors for acute pesticide poisoning, activating carbon fibers, and date pits for use in liver toxin adsorption. The second group of papers deals with forensic or analytical toxicology topics such as simplified methods for the analysis of gaseous toxic agents, rapid methods for the analysis and monitoring of pathogens in drinking water and water-based solutions, as well as the linkages between clinical and forensic toxicology. Each chapter presents new information on the topic discussed based on authors' experience while summarizing existing knowledge. As such, this book will be a good teaching aid and can be a prescribed or recommended reading for postgraduate students and professionals in the fields of public health, medicine, pharmacy, nursing, biology, toxicology, and forensic sciences.
Book Synopsis DNA Techniques to Verify Food Authenticity by : Malcolm Burns
Download or read book DNA Techniques to Verify Food Authenticity written by Malcolm Burns and published by Royal Society of Chemistry. This book was released on 2019-10-14 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: The food supply chain needs to reassure consumers and businesses about the safety and standards of food. Global estimates of the cost of food fraud to economies run into billions of dollars hence a huge surge in interest in food authenticity and means of detecting and preventing food fraud and food crime. Approaches targeting DNA markers have assumed a pre-eminence. This book is the most comprehensive and timely collection of material from those working at the forefront of DNA techniques applied to food authenticity. Addressing the new field of analytical molecular biology as it combines the quality assurance rigour of analytical chemistry with DNA techniques, it introduces the science behind DNA as a target analyte, its extraction, amplification, detection and quantitation as applied to the detection of food fraud and food crime. Making the link with traditional forensic DNA profiling and describing emerging and cutting-edge techniques such as next generation sequencing, this book presents real-world case studies from a wide perspective including from analytical service providers, industry, enforcement agencies and academics. It will appeal to food testing laboratories worldwide, who are just starting to use these techniques and students of molecular biology, food science and food integrity. Food policy professionals and regulatory organisations who will be using these techniques to back up legislation and regulation will find the text invaluable. Those in the food industry in regulatory and technical roles will want to have this book on their desks.
Download or read book Real-time PCR written by M Dorak and published by Garland Science. This book was released on 2007-01-24 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Book Synopsis Encyclopedia of Food Microbiology by : Carl A. Batt
Download or read book Encyclopedia of Food Microbiology written by Carl A. Batt and published by Academic Press. This book was released on 2014-04-02 with total page 3243 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the world's leading scientists and spanning over 400 articles in three volumes, the Encyclopedia of Food Microbiology, Second Edition is a complete, highly structured guide to current knowledge in the field. Fully revised and updated, this encyclopedia reflects the key advances in the field since the first edition was published in 1999 The articles in this key work, heavily illustrated and fully revised since the first edition in 1999, highlight advances in areas such as genomics and food safety to bring users up-to-date on microorganisms in foods. Topics such as DNA sequencing and E. coli are particularly well covered. With lists of further reading to help users explore topics in depth, this resource will enrich scientists at every level in academia and industry, providing fundamental information as well as explaining state-of-the-art scientific discoveries. This book is designed to allow disparate approaches (from farmers to processors to food handlers and consumers) and interests to access accurate and objective information about the microbiology of foods Microbiology impacts the safe presentation of food. From harvest and storage to determination of shelf-life, to presentation and consumption. This work highlights the risks of microbial contamination and is an invaluable go-to guide for anyone working in Food Health and Safety Has a two-fold industry appeal (1) those developing new functional food products and (2) to all corporations concerned about the potential hazards of microbes in their food products
Download or read book Ion Channel Factsbook written by and published by Academic Press. This book was released on 1995-11-16 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: How do you keep track of basic information on the proteins you work with? Where do you find details of their physicochemical properties, sequence information, gene organization? Are you tired of scanning review articles, primary papers and databases to locate that elusive fact? The Academic Press FactsBook series will satisfy scientists and clinical researchers suffering from information overload. Each volume provides a catalogue of the essential properties of families of molecules. Gene organization, sequence information, physicochemical properties, and biological activity are presented using a common, easy to follow format. Taken together they compile everything you wanted to know about proteins but were too busy to look for.In a set of four inter-related volumes, The Ion Channel FactsBook provides a comprehensive framework of facts about channel molecules central to electrical signalling phenomena in living cells. The first volume is devoted to Extracellular Ligand-Gated Integral Receptor-Channel Families including those molecular complexes activated by: 5-Hydroxytryptamine, ATP, Glutamate, Acetylcholine, GABA, Glycine. - Nomenclature - Expression - Sequence analyses - Structure and function - Electrophysiology - Pharmacology - Information retrieval
Book Synopsis Methods in Chemosensory Research by : Sidney A. Simon
Download or read book Methods in Chemosensory Research written by Sidney A. Simon and published by CRC Press. This book was released on 2001-09-26 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Chemosensory Research describes new molecular, electrophysiological, engineering, genetic, behavioral, psychophysical, and imaging techniques that have recently been adapted to investigate the basic neuronal mechanisms underlying chemoreception. Written by leaders in the field of neuronal chemoreception specializing in olfaction, gustation, baroreception, and chemical irritation caused by environmental pollutants, this unique book combines novel methods to investigate all aspects of taste and olfaction. These methods range from the molecular level to the investigation of individual cell taste and olfactory epithelia.
Book Synopsis Neuroscience Methods by : Rosemary Martin
Download or read book Neuroscience Methods written by Rosemary Martin and published by CRC Press. This book was released on 2019-08-16 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.
Book Synopsis Dopamine in the CNS I by : Gaetano Di Chiara
Download or read book Dopamine in the CNS I written by Gaetano Di Chiara and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts
Book Synopsis Gene Expression in the Central Nervous System by : Albert C. H. Yu
Download or read book Gene Expression in the Central Nervous System written by Albert C. H. Yu and published by Elsevier. This book was released on 1995 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene expression is an active ongoing process that maintains a functional CNS, as proteins are being made on a continual basis. Processes such as learning and memory, nerve cell repair and regeneration and its response to stress are critically dependent on gene expression. This volume highlights the role of gene expression in normal CNS function, and presents many research methods at the cutting edge of neuroscience, which will provide insight into therapeutic approaches through which the control of gene expression may be used in the treatment of many nervous system diseases.
Download or read book RT-PCR Protocols written by Nicola King and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
Book Synopsis Principles and Practices of Plant Genomics, Vol. 1 by : C Kole
Download or read book Principles and Practices of Plant Genomics, Vol. 1 written by C Kole and published by CRC Press. This book was released on 2024-11-01 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genome of an organism is depicted by genetic linkage mapping and physical mapping. Genome mapping stated with genetic linkage mapping and contributed enormously in genome analysis and its improvement. Physical mapping emerged later and is the prelude to structural and functional genomics. Volume One of Principles and Practices of Plant Genomics introduces the historical background of genome mapping, and delineates modern methods in the field. This volume briefly introduces the historical background and overview on genome mapping. Chapters deliberating on different types of molecular markers, their detection, relative merits, shortcomings and applications; types of mapping populations, methods of their generation, applications; basic concepts and schematic depiction of construction of genetic linkage maps; concepts and strategies of mapping genes controlling qualitative and quantitative traits on framework genetic linkage maps; rationale, methodologies and implications of comparative mapping; principles, strategies, and outcome of map-based cloning; overviews on the recent advances on plant genomics and genome initiatives; and finally computer strategies and software.
Book Synopsis Gene Expression Profiling by : Richard A. Shimkets
Download or read book Gene Expression Profiling written by Richard A. Shimkets and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.
Book Synopsis Essentials of Nucleic Acid Analysis by : Jacquie T Keer
Download or read book Essentials of Nucleic Acid Analysis written by Jacquie T Keer and published by Royal Society of Chemistry. This book was released on 2008-02-25 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade there has been a rapid development of molecular techniques, with an increasing range of instrumentation now available. The development of accompanying reference literature has not kept pace with technological advances and this poses significant challenges to the analyst. Essentials of Nucleic Acid Analysis sets out to guide the analyst through the steps needed to obtain good quality results in DNA analysis. The underlying principles for achieving this goal were formulated by LGC (formerly the Laboratory of the Government Chemist) as the six principles for ensuring valid analytical measurement, which are detailed in the introduction. The reader is also provided with guidelines for method validation and quality control of established and emerging DNA measurement techniques. The authors of each chapter are practitioners of the art of DNA analysis in areas where the quality of the result is critical. Technical details and examples of application of key techniques in nucleic acid analysis are provided while highlighting best practice, available standards and practical advice on improving measurement quality. This book provides an indispensable handbook and premier reference for those working in the widely varying areas and specifically in the fields of food analysis and forensic applications.
