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Partition Of Cell Partilces And Macromolecules
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Book Synopsis Aqueous Two-Phase Systems by : Harry Walter
Download or read book Aqueous Two-Phase Systems written by Harry Walter and published by Elsevier. This book was released on 1994-04-18 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Book Synopsis Methods for Affinity-Based Separations of Enzymes and Proteins by : Munishwar N. Gupta
Download or read book Methods for Affinity-Based Separations of Enzymes and Proteins written by Munishwar N. Gupta and published by Springer Science & Business Media. This book was released on 2002-04-01 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Book Synopsis Partitioning In Aqueous Two – Phase System by : Harry Walter
Download or read book Partitioning In Aqueous Two – Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Partition of Cell Particles and Macromolecules by : Per Åke Albertsson
Download or read book Partition of Cell Particles and Macromolecules written by Per Åke Albertsson and published by . This book was released on 1971 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Partition of Cell Particles and Macromolecules by : Per-Ake Albertsson
Download or read book Partition of Cell Particles and Macromolecules written by Per-Ake Albertsson and published by Wiley-Interscience. This book was released on 1986-03-28 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.
Book Synopsis Protein Purification by : Robert K. Scopes
Download or read book Protein Purification written by Robert K. Scopes and published by Springer Science & Business Media. This book was released on 1993-11-19 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
Book Synopsis Protein Structure Analysis by : Roza Maria Kamp
Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.
Book Synopsis Cell Separation by : Thomas G. Pretlow
Download or read book Cell Separation written by Thomas G. Pretlow and published by Academic Press. This book was released on 2014-05-10 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including liver cells, epidermal Langerhans cells, isolation of oval cells, clonal analysis, and the purification of polymorphonuclear leukocytes. Organized into 17 chapters, this volume begins with an overview of the central role of the liver in the metabolism of the body. This text then provides the analysis of Langerhans cells that allow modulation of their function and provide approaches to the treatment of skin disease. Other chapters consider the biological significance of oval cells. This book discusses as well the elucidation of the mechanisms of cellular proliferation, function, and differentiation in living tissues. The final chapter deals with the important applications of cell culture that involve continuous cell lines. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.
Book Synopsis Microcompartmentation and Phase Separation in Cytoplasm by :
Download or read book Microcompartmentation and Phase Separation in Cytoplasm written by and published by Academic Press. This book was released on 1999-10-21 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.This volume provides an overview of major cytoplasmic properties and events which including cytoarchitecture and the physical properties of cytoplasm, molecular compartmentation and gradients, channeling, sorting, and trafficking. It also addresses physicochemical events, both measured and anticipated, which attend solutions under conditions prevailing in cytoplasm: molecular crowding. It summarizes the current state of knowledge in the field and considers questions such as how molecules in cytoplasm interact.
Book Synopsis Aqueous Two-Phase Partitioning by : Boris Y. Zaslavsky
Download or read book Aqueous Two-Phase Partitioning written by Boris Y. Zaslavsky and published by CRC Press. This book was released on 1994-11-15 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
Book Synopsis Aqueous Two-Phase Systems by : Rajni Hatti-Kaul
Download or read book Aqueous Two-Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Book Synopsis Aqueous Biphasic Separations by : Robin D. Rogers
Download or read book Aqueous Biphasic Separations written by Robin D. Rogers and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of an American Chemical Society Symposium held in San Diego, California, March 13-14, 1994
Download or read book Advances in Protein Chemistry written by and published by Academic Press. This book was released on 1970-03-31 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Protein Chemistry
Book Synopsis Separations Using Aqueous Phase Systems by : D. Fisher
Download or read book Separations Using Aqueous Phase Systems written by D. Fisher and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.
Book Synopsis Steroid Receptors in Health and Disease by : Virinder Moudgil
Download or read book Steroid Receptors in Health and Disease written by Virinder Moudgil and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last two decades, progress in steroid hormone research has resulted in the development of new approaches to contraception as well as diagnosis and treatment of endocrine disorders and cancers. Although significant advances have been made in the purification, characterization, immunochemistry and molecular biology of steroid receptors, the precise molecular mechanism of steroid hormone action has remained obscure. This book captures the detailed presentations made at the first conference on Steroid Receptors in Health and Disease held at Meadow Brook Hall, Oakland University in the fall of 1987. The purpose of this international con ference was to facilitate scientific exchange toward a better understand ing of the mode of action of steroid hormones. The scientific sessions consisted of poster presentations and state-of-the-art lectures, the latter of which make up this volume. The first chapter is meant to provide the reader with a more general background of the topics covered in the book, as well as to discuss certain theme-related issues that are either not yet well-established or accepted or are in the stage of infancy. It is hoped that this volume will serve as a useful treatise for students and investigators interested in basic and clinical aspects of biological regulation by steroid hormones. A task of this magnitude could not have been undertaken without the encouragement, advice and continued generous assistance of the members of the scientific committee. I am gratefully indebted to Drs.
Book Synopsis Heme, Chlorophyll, and Bilins by : Alison Smith
Download or read book Heme, Chlorophyll, and Bilins written by Alison Smith and published by Springer Science & Business Media. This book was released on 2002 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although researchers can profitably investigate heme, chlorophyll, and related tetrapyrroles in a wide range of academic and medical research programs, the handling and manipulation of these delicate compounds requires considerable skill and cross-boundary knowledge. In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators overcomes these limitations by describing in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and the analysis of chlorophyll and bilins. Timely and highly practical, Heme, Chlorophyll, and Bilins: Methods and Protocols is a gold-standard collection of readily reproducible techniques suitable for a wide range of researchers, whether it be a clinician studying photodynamic therapy, an ecologist studying the chlorophyll composition of leaves in a tropical forest, or a cell biologist investigating the function of specific hemoproteins.
