Nonisotopic Dna Probe Techniques

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Nonisotopic Probing, Blotting, and Sequencing

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Author : Larry J. Kricka
Publisher : Elsevier
ISBN 13 : 0080537669
Total Pages : 539 pages
Book Rating : 4.0/5 (85 download)

Book Synopsis Nonisotopic Probing, Blotting, and Sequencing by : Larry J. Kricka

Download or read book Nonisotopic Probing, Blotting, and Sequencing written by Larry J. Kricka and published by Elsevier. This book was released on 1995-06-01 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection

Nonisotopic DNA Probe Techniques

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Author : Larry J. Kricka
Publisher : Academic Press
ISBN 13 : 0323137717
Total Pages : 373 pages
Book Rating : 4.3/5 (231 download)

Book Synopsis Nonisotopic DNA Probe Techniques by : Larry J. Kricka

Download or read book Nonisotopic DNA Probe Techniques written by Larry J. Kricka and published by Academic Press. This book was released on 2012-12-02 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided.This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection.Gives extensive practical informationCovers major types of nonisotopic labels and proceduresPresents background information for each methodProvides strategies and detailed experimental procedures for labeling and detecting DNA sequences byFluorescenceChemiluminescenceBioluminescenceColorimetry

DNA Technology in Forensic Science

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Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309045878
Total Pages : 199 pages
Book Rating : 4.3/5 (9 download)

Book Synopsis DNA Technology in Forensic Science by : National Research Council

Download or read book DNA Technology in Forensic Science written by National Research Council and published by National Academies Press. This book was released on 1992-02-01 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.

Evaluation Technologies for Food Quality

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Author : Jian Zhong
Publisher : Woodhead Publishing
ISBN 13 : 0128142189
Total Pages : 884 pages
Book Rating : 4.1/5 (281 download)

Book Synopsis Evaluation Technologies for Food Quality by : Jian Zhong

Download or read book Evaluation Technologies for Food Quality written by Jian Zhong and published by Woodhead Publishing. This book was released on 2019-04-16 with total page 884 pages. Available in PDF, EPUB and Kindle. Book excerpt: Evaluation Technologies for Food Quality summarizes food quality evaluation technologies, which include sensory evaluation techniques and chemical and physical analysis. In particular, the book introduces many novel micro and nano evaluation techniques, such as atomic force microscopy, scanning electron microscopy, and other nanomaterial-based methods. All topics cover basic principles, procedures, advantages, limitations, recent technology development, and application progress in different types of foods. This book is a valuable resource for scientists in the field of food science, engineering, and professionals in the food industry, as well as for undergraduate and postgraduate students studying food quality evaluation technology. - Explains basic principles, procedures, advantages, limitations, and current applications of recent food quality technologies - Provides guidance on the understanding and application of food quality evaluation technology in the field of food research and food industry - Introduces many novel micro/nano evaluation techniques, such as atomic force and scanning electron microscopies and other nanomaterial-based methods

Molecular Beacons

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Author : Chaoyong James Yang
Publisher : Springer Science & Business Media
ISBN 13 : 3642391095
Total Pages : 201 pages
Book Rating : 4.6/5 (423 download)

Book Synopsis Molecular Beacons by : Chaoyong James Yang

Download or read book Molecular Beacons written by Chaoyong James Yang and published by Springer Science & Business Media. This book was released on 2013-08-15 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.

Biotechnology in Medicine and Agriculture

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Author : Anil Kumar
Publisher :
ISBN 13 : 9789381141403
Total Pages : 0 pages
Book Rating : 4.1/5 (414 download)

Book Synopsis Biotechnology in Medicine and Agriculture by : Anil Kumar

Download or read book Biotechnology in Medicine and Agriculture written by Anil Kumar and published by . This book was released on 2012-11-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examines the role of biotechnology in research and advances in agriculture, veterinary, biomedical, industrial development and environmental protection. It presents the basics of the potential for biotechnology in each of these areas in understandable language. The book aims to give students a complete view of the 'know-how' of biotechnology.

Methods in Microbiology

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Author :
Publisher : Academic Press
ISBN 13 : 0128211466
Total Pages : 216 pages
Book Rating : 4.1/5 (282 download)

Book Synopsis Methods in Microbiology by :

Download or read book Methods in Microbiology written by and published by Academic Press. This book was released on 2021-07-07 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book Methods in Silkworm Microbiology is the first ever publication that provides in-depth reviews on the latest progresses about silkworm –pathogen interactions, diseases and management practices for sustainable development of sericulture. Different molecular and immunodiagnostic methods for the detection of pathogens have been comprehensively addressed. Most recent advancements on the role of Micro RNAs in silkworm and pathogen interactions are provided with suitable illustrations. Recent technological advances and emerging trends in exploring silkworm gut microbial communities towards translation research, particularly to understand microbiome functions have been highlighted. Information on various immune mechanisms of silkworm against invading pathogens is summarized. The book further highlights the silkworm gut microbiota as a potential source for biotechnological applications. - Provide comprehensive reviews and valuable methods from the selected experts on the topic "Methods in silkworm microbiology/pathology" - Provides latest information on application of genomics and transcriptomics to decipher silkworm gut microbial communities. Different molecular and immunodiagnostic methods for the detection of pathogens have been comprehensively addressed - Provides up to date information on silkworm-pathogen interactions, different silkworm diseases and immune mechanisms

Molecular Microbial Ecology Manual

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Author : Antoon D. L. Akkermans
Publisher : Springer
ISBN 13 : 9400902158
Total Pages : 266 pages
Book Rating : 4.4/5 (9 download)

Book Synopsis Molecular Microbial Ecology Manual by : Antoon D. L. Akkermans

Download or read book Molecular Microbial Ecology Manual written by Antoon D. L. Akkermans and published by Springer. This book was released on 2014-01-13 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.

RNA Methodologies

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Author : Robert E. Farrell Jr.
Publisher : Elsevier
ISBN 13 : 0080454763
Total Pages : 794 pages
Book Rating : 4.0/5 (84 download)

Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.

Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Nucleic Acids

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Author : Marcelo Larramendy
Publisher : BoD – Books on Demand
ISBN 13 : 9535122649
Total Pages : 222 pages
Book Rating : 4.5/5 (351 download)

Book Synopsis Nucleic Acids by : Marcelo Larramendy

Download or read book Nucleic Acids written by Marcelo Larramendy and published by BoD – Books on Demand. This book was released on 2016-03-16 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.

Molecular Methods for Virus Detection

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Author : Danny L. Wiedbrauk
Publisher : Elsevier
ISBN 13 : 0080536891
Total Pages : 405 pages
Book Rating : 4.0/5 (85 download)

Book Synopsis Molecular Methods for Virus Detection by : Danny L. Wiedbrauk

Download or read book Molecular Methods for Virus Detection written by Danny L. Wiedbrauk and published by Elsevier. This book was released on 1995-02-08 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables

In Situ Hybridization Protocols

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Author : Ian A. Darby
Publisher : Taylor & Francis US
ISBN 13 : 9780896036864
Total Pages : 368 pages
Book Rating : 4.0/5 (368 download)

Book Synopsis In Situ Hybridization Protocols by : Ian A. Darby

Download or read book In Situ Hybridization Protocols written by Ian A. Darby and published by Taylor & Francis US. This book was released on 2000 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Handbook of Fluorescent Dyes and Probes

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Author : R. W. Sabnis
Publisher : John Wiley & Sons
ISBN 13 : 1118028694
Total Pages : 468 pages
Book Rating : 4.1/5 (18 download)

Book Synopsis Handbook of Fluorescent Dyes and Probes by : R. W. Sabnis

Download or read book Handbook of Fluorescent Dyes and Probes written by R. W. Sabnis and published by John Wiley & Sons. This book was released on 2015-06-02 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: A COMPLETE, UP-TO-DATE RESOURCE OF INFORMATION ON MORE THAN 150 FLUORESCENT DYES AND PROBES Handbook of Fluorescent Dyes and Probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in various fields. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, color, material, physical and related allied fields. The ease of use of the handbook is further enhanced by various appendixes provided at the end of the book to conveniently and easily locate the dye as per the reader's need. This is the first book to give the CAS registry numbers, chemical structure, Chemical Abstract (CA) index name, all other chemical names, Merck Index number, chemical/dye class, molecular formula, molecular weight, physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient, and quantum yield of fluorescent dyes and probes, as well as to provide access to synthetic procedures (lab scale and industrial scale) of dyes and probes in a single source. This user-friendly handbook also features references on safety, toxicity and adverse effects of dyes and probes on humans, animals and the environment, including: acute/chronic toxicity aquatic toxicity carcinogenicity cytotoxicity ecotoxicity genotoxocity hematotoxicity hepatotoxicity immunotoxicity marine toxicity microbial toxicity mutagenicity nephrotoxicity neurotoxicity nucleic acid damage oral toxicity phototoxicity phytotoxicity reproductive toxicity skin toxicity Containing imaging/labeling applications, biological/medical applications and industrial applications, Handbook of Fluorescent Dyes and Probes is a convenient, vital resource for industrial and academic researchers, and a valuable desktop reference for medical professionals, lab supervisors, scientists, chemists, biologists, engineers, physicists, intellectual property professionals, students, and professors. Includes all fluorescent dyes & probes known to date and provides a complete, up-to-date library of information in one reference/handbook Includes more than 300 fluorescent dyes & probes organized alphabetically by the commonly used Chemical Name Provides access to synthesis procedures (lab scale and industrial scale) of fluorescent dyes & probes First book to provide references on safety, toxicity and adverse effects of fluorescent dyes and probes on humans, animals, and the environment User-friendly and convenient resource guide for chemical, biological, medical, and intellectual property professionals in a broad range of disciplines

Human Biochemistry

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Author : Gerald Litwack
Publisher : Academic Press
ISBN 13 : 032391053X
Total Pages : 883 pages
Book Rating : 4.3/5 (239 download)

Book Synopsis Human Biochemistry by : Gerald Litwack

Download or read book Human Biochemistry written by Gerald Litwack and published by Academic Press. This book was released on 2021-11-28 with total page 883 pages. Available in PDF, EPUB and Kindle. Book excerpt: **Selected for Doody's Core Titles® 2024 in Biochemistry** Human Biochemistry, Second Edition provides a comprehensive, pragmatic introduction to biochemistry as it relates to human development and disease. Here, Gerald Litwack, award-wining researcher and longtime teacher, discusses the biochemical aspects of organ systems and tissue, cells, proteins, enzymes, insulins and sugars, lipids, nucleic acids, amino acids, polypeptides, steroids, and vitamins and nutrition, among other topics. Fully updated to address recent advances, the new edition features fresh discussions on hypothalamic releasing hormones, DNA editing with CRISPR, new functions of cellular prions, plant-based diet and nutrition, and much more. Grounded in problem-driven learning, this new edition features clinical case studies, applications, chapter summaries, and review-based questions that translate basic biochemistry into clinical practice, thus empowering active clinicians, students and researchers. - Presents an update on a past edition winner of the 2018 Most Promising New Textbook (College) Award (Texty) from the Textbook and Academic Authors Association and the PROSE Award of the Association of American Publishers - Provides a fully updated resource on current research in human and medical biochemistry - Includes clinical case studies, applications, chapter summaries and review-based questions - Adopts a practice-based approach, reflecting the needs of both researchers and clinically oriented readers

Guideline for Salinity Assessment, Mitigation and Adaptation Using Nuclear and Related Techniques

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Author : Mohammad Zaman
Publisher : Springer
ISBN 13 : 331996190X
Total Pages : 183 pages
Book Rating : 4.3/5 (199 download)

Book Synopsis Guideline for Salinity Assessment, Mitigation and Adaptation Using Nuclear and Related Techniques by : Mohammad Zaman

Download or read book Guideline for Salinity Assessment, Mitigation and Adaptation Using Nuclear and Related Techniques written by Mohammad Zaman and published by Springer. This book was released on 2018-11-28 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book is an outcome of the collaboration between the Soil and Water Management & Crop Nutrition Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency (IAEA), Vienna, Austria, and Dr. Shabbir A Shahid, Senior Salinity Management Expert, Freelancer based in United Arab Emirates.The objective of this book is to develop protocols for salinity and sodicity assessment and develop mitigation and adaptation measures to use saline and sodic soils sustainably. The focus is on important issues related to salinity and sodicity and to describe these in an easy and user friendly way. The information has been compiled from the latest published literature and from the authors’ publications specific to the subject matter. The book consists of six chapters. Chapter 1 introduces the terms salinity and sodicity and describes various salinity classification systems commonly used around the world. Chapter 2 reviews global distribution of salinization and socioeconomic aspects related to salinity and crop production. Chapters 3 covers comprehensively salinity and sodicity adaptation and mitigation options including physical, chemical, hydrological and biological methods. Chapter 4 discusses the efforts that have been made to demonstrate the development of soil salinity zones under different irrigation systems. Chapter 5 discusses the quality of irrigation water, boron toxicity and relative tolerance to boron, the effects of chlorides on crops. Chapter 6 introduces the role of nuclear techniques in saline agriculture.

In Situ Hybridization Methods

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Author : Giselbert Hauptmann
Publisher : Humana
ISBN 13 : 9781493944637
Total Pages : 0 pages
Book Rating : 4.9/5 (446 download)

Book Synopsis In Situ Hybridization Methods by : Giselbert Hauptmann

Download or read book In Situ Hybridization Methods written by Giselbert Hauptmann and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Antigen Retrieval Techniques

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Author : Shan-Rong Shi
Publisher : Eaton Publishing Company/Biotechniques Books
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.3/5 (91 download)

Book Synopsis Antigen Retrieval Techniques by : Shan-Rong Shi

Download or read book Antigen Retrieval Techniques written by Shan-Rong Shi and published by Eaton Publishing Company/Biotechniques Books. This book was released on 2000 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: