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Modifications And Targeting Of Protein Termini Part A
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Book Synopsis Modifications and Targeting of Protein Termini Part A by :
Download or read book Modifications and Targeting of Protein Termini Part A written by and published by Elsevier. This book was released on 2023-05-23 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more. Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology serials - Updated release includes the latest information on Modifications and Targeting of Protein Termini
Book Synopsis Modifications and targeting of protein termini Part B by :
Download or read book Modifications and targeting of protein termini Part B written by and published by Elsevier. This book was released on 2023-07-31 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modifications and Targeting of Protein Termini, Part B, Volume 686 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including In vitro production of N-degron fused proteins and its application, Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomics, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathway. Other chapters cover Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, Building libraries to dissect terminal degrons with fluorescent timers, Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS, Probing the effects of N-terminal acetylation on a-synuclein structure, aggregation and toxicity, Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE), and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Modifications and Targeting of Protein Termini
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Neuroproteomics written by Oscar Alzate and published by CRC Press. This book was released on 2009-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Book Synopsis Membrane Dynamics and Domains by : Peter J. Quinn
Download or read book Membrane Dynamics and Domains written by Peter J. Quinn and published by Springer Science & Business Media. This book was released on 2004-07-02 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fluid-mosaic model of membrane structure formulated by Singer and Nicolson in the early 1970s has proven to be a durable concept in terms of the principles governing the organization of the constituent lipids and proteins. During the past 30 or so years a great deal of information has accumulated on the composition of various cell membranes and how this is related to the dif ferent functions that membranes perform. Nevertheless, the task of explaining particular functions at the molecular level has been hampered by lack of struc tural detail at the atomic level. The reason for this is primarily the difficulty of crystallizing membrane proteins which require strategies that differ from those used to crystallize soluble proteins. The unique exception is bacteriorhodopsin of the purple membrane of Halobacterium halobium which is interpolated into a membrane that is neither fluid nor in a mosaic configuration. To date only 50 or so membrane proteins have been characterised to atomic resolution by diffraction methods, in contrast to the vast data accumulated on soluble proteins. Another factor that has been difficult to explain is the reason why the lipid compliment of membranes is often extremely complex. Many hundreds of different molecular species of lipid can be identified in some membranes. Remarkably, the particular composition of each membrane appears to be main tained within relatively narrow limits and its identity distinguished from other morphologically-distinct membranes.
Book Synopsis Bioconjugate Techniques by : Greg T. Hermanson
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Book Synopsis Modifications and Targeting of Protein Termini Part A by :
Download or read book Modifications and Targeting of Protein Termini Part A written by and published by Elsevier. This book was released on 2023-07 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more. Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more.
Book Synopsis Post-Translational Modifications in Health and Disease by : Cecilio J. Vidal
Download or read book Post-Translational Modifications in Health and Disease written by Cecilio J. Vidal and published by Springer Science & Business Media. This book was released on 2010-10-13 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Post-translational modifications serve many different purposes in several cellular processes such as gene expression, protein folding and transport to appropriate cell compartment, protein-lipid and protein-protein interactions, enzyme regulation, signal transduction, cell proliferation and differentiation, protein stability, recycling and degradation. Although several-hundred different modifications are known, the significance of many of them remains unknown. The enormous versatility of the modifications which frequently alter the physico-chemical properties of the respective proteins represents an extraordinary challenge in understanding their physiological role. Since essential cellular functions are regulated by protein modifications, an improvement of current understanding of their meaning might allow new avenues to prevent and/or alleviate human and animal diseases.
Book Synopsis Expressed Protein Ligation by : Miquel Vila-Perelló
Download or read book Expressed Protein Ligation written by Miquel Vila-Perelló and published by Humana. This book was released on 2020-03-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Book Synopsis How Proteins Work by : Michael Williamson
Download or read book How Proteins Work written by Michael Williamson and published by Garland Science. This book was released on 2012-03-26 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-throughputomics' projects such as genome sequencing, structural genomics and proteomics mean that there is no shortage of information on proteins. But the more information we have, the harder it is to make sense of it, to know where to start, and to identify the important results. This book is a clear, up to date and authoritative account of
Book Synopsis Membrane Biogenesis by : Jos A.F. Op den Kamp
Download or read book Membrane Biogenesis written by Jos A.F. Op den Kamp and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.
Book Synopsis Essentials of Glycobiology by : Ajit Varki
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Book Synopsis Cell Chemistry and Physiology: Part II by : Edward Bittar
Download or read book Cell Chemistry and Physiology: Part II written by Edward Bittar and published by Elsevier. This book was released on 1996-05-14 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume illustrates the extent to which the traditional distinction between biochemical and physiological processes is being obliterated by molecular biology. It can hardly be doubted that the revolution in cell and molecular biology is leading to core knowledge that provides an outline of the integrative and reductionist approach. We view this as the beginning of a new era, that of the integration of learning.As in the preceding volumes, the choice of topics has been deliberate not only because of the need to keep the volume within reasonable bounds but also because of the need to avoid information over-load. Several relevant topics are dealt with in other modules; for example, the role of G proteins in transmembrane signalling is covered in the Membranes and Cell Signalling module (i.e., Volume 7). Omissions are of course inevitable but they are minor. A case in point is the subject of phosphatases, the treatment of which does not take into account calcineurin. One of the key functions of this Ca2+ -activated protein phosphatase that is also regulated by calmodulin is to desphosphorylate voltage-dependent Ca2+ channels. The mere recognition of such omissions before or after consulting textbooks and journals should be a spur to a more complete discussion by the student of the subject in a small group teaching setting.
Download or read book Protein Prenylation, Part B written by and published by Academic Press. This book was released on 2011-11-08 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of The Enzymes features high-caliber thematic articles on the topic of glycosylphosphatidylinositol (GPI) anchoring of proteins. - Contributions from leading authorities - Informs and updates on all the latest developments in the field
Book Synopsis The Proteomics Protocols Handbook by : John M. Walker
Download or read book The Proteomics Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2007-10-09 with total page 969 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Book Synopsis The Ubiquitin System by : Milton J. Schlesinger
Download or read book The Ubiquitin System written by Milton J. Schlesinger and published by Cold Spring Harbor Laboratory Press. This book was released on 1988 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Seed Technology and Its Biological Basis by : Michael Black
Download or read book Seed Technology and Its Biological Basis written by Michael Black and published by CRC Press. This book was released on 2000 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited by a renowned seed biologist with a team assembled from the most respected laboratories worldwide, Seed Technology and Its Biological Basis illustrates the commercial value of seeds as a major resource. The editors provide a sweeping overview of the current state-of-the-art in seed technology and its biological basis. The book is invaluable to researchers and professionals in both the industrial and academic sectors.
