Mixed Mode Chromatography

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Mixed-Mode Chromatography

Author : Qian-Hong Wan
Publisher : Springer Nature
ISBN 13 : 9811654859
Total Pages : 491 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis Mixed-Mode Chromatography by : Qian-Hong Wan

Download or read book Mixed-Mode Chromatography written by Qian-Hong Wan and published by Springer Nature. This book was released on 2021-11-23 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.

Mixed Mode Chromatography: Column Evaluation of a Reversed Phase Anion Exchange Column in HPLC.

Author : Annmarie Brogan
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)

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Book Synopsis Mixed Mode Chromatography: Column Evaluation of a Reversed Phase Anion Exchange Column in HPLC. by : Annmarie Brogan

Download or read book Mixed Mode Chromatography: Column Evaluation of a Reversed Phase Anion Exchange Column in HPLC. written by Annmarie Brogan and published by . This book was released on 1993 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Chromatography

Author : Dermot Walls
Publisher : Humana Press
ISBN 13 : 9781607619147
Total Pages : 527 pages
Book Rating : 4.6/5 (191 download)

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Book Synopsis Protein Chromatography by : Dermot Walls

Download or read book Protein Chromatography written by Dermot Walls and published by Humana Press. This book was released on 2011-04-06 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Application of Mixed Mode Chromatography for Separation of Biologically Important Molecules

Author : Anja Kristl
Publisher :
ISBN 13 :
Total Pages : 128 pages
Book Rating : 4.:/5 (128 download)

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Book Synopsis Application of Mixed Mode Chromatography for Separation of Biologically Important Molecules by : Anja Kristl

Download or read book Application of Mixed Mode Chromatography for Separation of Biologically Important Molecules written by Anja Kristl and published by . This book was released on 2021 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Purification Tools for Monoclonal Antibodies

Author : Pete Gagnon
Publisher : Validated Biosystems Incorporated
ISBN 13 :
Total Pages : 278 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Purification Tools for Monoclonal Antibodies by : Pete Gagnon

Download or read book Purification Tools for Monoclonal Antibodies written by Pete Gagnon and published by Validated Biosystems Incorporated. This book was released on 1996 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purification Tools for Monoclonal is an essential book for professionals, educators and advanced students in the field of Biotechnology. It is based on experience gained from purification process development, scale-up, and manufacture of more than 250 monoclonal-based diagnostic and therapeutic products. Ten chapters provide in-depth coverage of major separation mechanisms, process strengths, weaknesses and method development; all fully integrated with the special performance, economic and validation requirements associated with monoclonals. Covered methods include precipitation with inorganic salts, polyethylene glycol, electrolyte depletion, caprylic acid, ethacridine, chromatographic purification by size exclusion, ion exchange, hydroxyapatite, hydrophobic interaction, immobilized metal affinity, hydrophilic interaction, euglobulin adsorption, thiophilic adsorption, protein A, protein G, lectin affinity, and more. 88 figures, 29 tables.

Perspective Chapter

Author : Ngoc-Van Thi Nguyen
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (139 download)

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Book Synopsis Perspective Chapter by : Ngoc-Van Thi Nguyen

Download or read book Perspective Chapter written by Ngoc-Van Thi Nguyen and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this chapter, we present mixed-mode stationary phases and their applications in the determination of nonpolar, polar, and charged compounds, as well as larger molecules such as peptides or proteins using a single column. Mixed-mode chromatography (MMC) has been growing rapidly in recent years, owing to the new generation of mixed-mode stationary phases and a better understanding of multimode interactions. Mixed-mode chromatography provides a wide range of selectivities and adequate retention of a variety of compounds, especially polar and charged molecules. In summary, this technique is particularly useful in the pharmaceutical analysis of drugs, impurities, biopharmaceuticals, and polar compounds in natural products.

Protein Downstream Processing

Author : Nikolaos E. Labrou
Publisher : Humana
ISBN 13 : 9781493960408
Total Pages : 0 pages
Book Rating : 4.9/5 (64 download)

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Book Synopsis Protein Downstream Processing by : Nikolaos E. Labrou

Download or read book Protein Downstream Processing written by Nikolaos E. Labrou and published by Humana. This book was released on 2016-09-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

Author : Michal Holcapek
Publisher : Elsevier
ISBN 13 : 0128117338
Total Pages : 522 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Handbook of Advanced Chromatography /Mass Spectrometry Techniques by : Michal Holcapek

Download or read book Handbook of Advanced Chromatography /Mass Spectrometry Techniques written by Michal Holcapek and published by Elsevier. This book was released on 2017-09-07 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. - Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more - Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques - Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research

Process Scale Purification of Antibodies

Author : Uwe Gottschalk
Publisher : John Wiley & Sons
ISBN 13 : 1119126932
Total Pages : 849 pages
Book Rating : 4.1/5 (191 download)

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Book Synopsis Process Scale Purification of Antibodies by : Uwe Gottschalk

Download or read book Process Scale Purification of Antibodies written by Uwe Gottschalk and published by John Wiley & Sons. This book was released on 2017-03-07 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing

Mixed-mode Interaction Membrane Chromatography for Protein Fractionation

Author : Nur Farahain Khusnun
Publisher :
ISBN 13 :
Total Pages : 39 pages
Book Rating : 4.:/5 (953 download)

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Book Synopsis Mixed-mode Interaction Membrane Chromatography for Protein Fractionation by : Nur Farahain Khusnun

Download or read book Mixed-mode Interaction Membrane Chromatography for Protein Fractionation written by Nur Farahain Khusnun and published by . This book was released on 2012 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nowadays, membrane chromatography has widely use in protein separation. Most of the membrane chromatography is prepared through a chemical modification which sometimes can changes the structure and properties of the membrane itself. In this study, membrane chromatography was prepared through physical modification using the mixed matrix membrane preparation concept. Amberlite IR120 cation exchange resin (CEX) and Lewatit MP500 anion exchange resin (AEX) was used to formulate mixed mode interaction membrane chromatography (MMIMC) based on ethylene vinyl alcohol base membrane matrix. The total resin loading from 20-50% and the ratio between anion exchange resin and cation exchange resin was studied. This research found the optimum resin loading is 30%. Pure AEX MMM has the binding capacity of 340.26 mg BSA/g membrane and pure CEX MMM has a binding capacity of 3166.22 mg LZY/g membrane. The MMIMC with ratio CEX/AEX equal to 50/50 has a capacity of 895.898 mg LZY/g membrane and 3430.74 mg BSA/g membrane. A high performance MMIMC can be produced by optimization the loading percentage of ion exchanger resin and the ratio of CEX/AEX resin incorporate into the membrane.

Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications

Author : Perry G. Wang
Publisher : CRC Press
ISBN 13 : 1439807531
Total Pages : 613 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications by : Perry G. Wang

Download or read book Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications written by Perry G. Wang and published by CRC Press. This book was released on 2011-02-17 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.

Advances in Chromatography, Volume 47

Author : Eli Grushka
Publisher : CRC Press
ISBN 13 : 1420060376
Total Pages : 470 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Advances in Chromatography, Volume 47 by : Eli Grushka

Download or read book Advances in Chromatography, Volume 47 written by Eli Grushka and published by CRC Press. This book was released on 2009-02-23 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: For more than four decades, scientists and researchers have relied upon the Advances in Chromatography Series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. Covering the state of the art in separation science, this volume continues to present timely, cutting-edge reviews on chromatog

Ion-Exchange Chromatography and Related Techniques

Author : Pavel Nesterenko
Publisher : Elsevier
ISBN 13 : 0443153701
Total Pages : 646 pages
Book Rating : 4.4/5 (431 download)

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Book Synopsis Ion-Exchange Chromatography and Related Techniques by : Pavel Nesterenko

Download or read book Ion-Exchange Chromatography and Related Techniques written by Pavel Nesterenko and published by Elsevier. This book was released on 2023-11-15 with total page 646 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion-Exchange Chromatography and Related Techniques defines the current state-of-the-art in ion-exchange chromatography and related techniques and their implementation in laboratory and industrial practice. This book provides a compact source of information to facilitate the transfer of knowledge and experience acquired by separation science specialists to colleagues from diverse backgrounds who need to acquire fundamental and practical information to facilitate progress in research and management functions reliant on information acquired by separation. Individual chapters written by recognized experts lending credibility to the work will allow this book to serve as a high value reference source of current information for analytical and biopharmaceutical chemists. - Includes individual chapters written by recognized authoritative and visionary experts in the field to provide an overview and focused treatment of a single topic - Presents comprehensive coverage of ion-exchange techniques from theory, to methods, to selected applications for ions and biopolymers - Provides Tables and diagrams with commonly used data to facilitate practical work, comparison of results and decision-making

Aromatic Amino Acids—Advances in Research and Treatment: 2013 Edition

Author :
Publisher : ScholarlyEditions
ISBN 13 : 1481685244
Total Pages : 277 pages
Book Rating : 4.4/5 (816 download)

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Book Synopsis Aromatic Amino Acids—Advances in Research and Treatment: 2013 Edition by :

Download or read book Aromatic Amino Acids—Advances in Research and Treatment: 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aromatic Amino Acids—Advances in Research and Treatment: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Phenylalanine. The editors have built Aromatic Amino Acids—Advances in Research and Treatment: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Phenylalanine in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Aromatic Amino Acids—Advances in Research and Treatment: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Mixed-Mode Hydrophilic and Ionic Interaction Chromatography Rivals Reversed-Phased Chromatography for the Separation of Peptides

Download Mixed-Mode Hydrophilic and Ionic Interaction Chromatography Rivals Reversed-Phased Chromatography for the Separation of Peptides PDF Online Free

Author : B. Y. Zhu
Publisher :
ISBN 13 :
Total Pages : 3 pages
Book Rating : 4.:/5 (228 download)

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Book Synopsis Mixed-Mode Hydrophilic and Ionic Interaction Chromatography Rivals Reversed-Phased Chromatography for the Separation of Peptides by : B. Y. Zhu

Download or read book Mixed-Mode Hydrophilic and Ionic Interaction Chromatography Rivals Reversed-Phased Chromatography for the Separation of Peptides written by B. Y. Zhu and published by . This book was released on 1992 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Essentials in Modern HPLC Separations

Author : Serban C. Moldoveanu
Publisher : Elsevier
ISBN 13 : 0323998364
Total Pages : 718 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Essentials in Modern HPLC Separations by : Serban C. Moldoveanu

Download or read book Essentials in Modern HPLC Separations written by Serban C. Moldoveanu and published by Elsevier. This book was released on 2022-06-24 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated. Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry. - Provides a thoroughly updated resource, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more - Includes insights into the chromatographic process to find the optimum solution for analyzing complex samples - Presents a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, beverage and environmental samples

Advances in Chromatography

Author : Eli Grushka
Publisher : CRC Press
ISBN 13 : 1439858446
Total Pages : 480 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Advances in Chromatography by : Eli Grushka

Download or read book Advances in Chromatography written by Eli Grushka and published by CRC Press. This book was released on 2012-01-27 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: For more than four decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. For Volume 50, the series editors have invited established, well-known chemists from across the globe to offer cutting-edge reviews on their areas of expertise. The clear presentation of topics and vivid illustrations for which this series has become known makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill.