Microrna Expression Detection Methods

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MicroRNA Expression Detection Methods

Author : Zhiguo Wang
Publisher : Springer
ISBN 13 : 3642049281
Total Pages : 382 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis MicroRNA Expression Detection Methods by : Zhiguo Wang

Download or read book MicroRNA Expression Detection Methods written by Zhiguo Wang and published by Springer. This book was released on 2009-12-23 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quanti?cation; a wide spectrum of creative and inno- tive techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improve the existing techniques and develop more new methods to ?t to new application needs. These considerations prompted us and urged us to undertake the work: writing a book focusing on miRNA expression detection methods.

MicroRNA Detection and Target Identification

Author : Tamas Dalmay
Publisher : Springer Nature
ISBN 13 : 1071629824
Total Pages : 267 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis MicroRNA Detection and Target Identification by : Tamas Dalmay

Download or read book MicroRNA Detection and Target Identification written by Tamas Dalmay and published by Springer Nature. This book was released on 2023-01-23 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

MicroRNA Detection and Pathological Functions

Author : Xueji Zhang
Publisher : Springer
ISBN 13 : 3662472937
Total Pages : 108 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis MicroRNA Detection and Pathological Functions by : Xueji Zhang

Download or read book MicroRNA Detection and Pathological Functions written by Xueji Zhang and published by Springer. This book was released on 2015-05-26 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

MicroRNA Methods

Author : John J. Rossi
Publisher : Academic Press
ISBN 13 : 0080551394
Total Pages : 302 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis MicroRNA Methods by : John J. Rossi

Download or read book MicroRNA Methods written by John J. Rossi and published by Academic Press. This book was released on 2007-08-30 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process – or stops it – is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.

Luminescence-based MicroRNA Detection Methods

Author : Kyle A. Cissell
Publisher :
ISBN 13 :
Total Pages : 250 pages
Book Rating : 4.:/5 (88 download)

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Book Synopsis Luminescence-based MicroRNA Detection Methods by : Kyle A. Cissell

Download or read book Luminescence-based MicroRNA Detection Methods written by Kyle A. Cissell and published by . This book was released on 2010 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs (miRNA) are short, 18-24 nucleotide long noncoding RNAs. These small RNAs, which are initially transcribed in the nucleus, are transported into the cell cytoplasm where they regulate protein translation either through direct cleavage of mRNA, or indirect inhibition through binding to mRNA and disrupting the protein translation machinery. Recently, miRNAs have gained much attention due to their implication in numerous diseases and cancers. It has been found that heightened or lowered levels of miRNA in diseased cells vs. healthy cells are linked to disease progression. It is therefore immensely important to be able to detect these small molecules. Current detection methods of Northern blotting, microarrays, and qRT-PCR suffer from drawbacks including low sensitivity, a lack of simplicity, being semi-quantitative in nature, time-consuming, and requiring expensive instruments. This work aims to develop novel miRNA technologies which will address these above problems. Bioluminescent labels are promising alternatives to current methods of miRNA detection. Bioluminescent labels are relatively small, similar in size to fluorescent proteins, and they emit very intense signals upon binding to their substrate. Bioluminescent labels are advantageous to fluorescent labels in that they do not require an external excitation source, rather, the excitation energy is supplied through a biochemical reaction. Therefore, background signal due to excitation is eliminated. They also have the advantage of being produced in large amounts through bacterial expression. Four miRNA detection methods are presented which utilize luminescence-based methods. Three employ Renilla luciferase, a bioluminescent protein, and one is based on fluorescence. The presented methods are capable of detecting miRNA from the picomole (nanomolar) level down to the femtomole (picomolar) level. These methods are rapid, sensitive, simple, and quantitative, can be employed in complex matrices, and do not require expensive instruments. All methods are hybridization-based and do not require amplification steps.

In Situ Hybridization Methods

Author : Giselbert Hauptmann
Publisher : Humana
ISBN 13 : 9781493944637
Total Pages : 0 pages
Book Rating : 4.9/5 (446 download)

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Book Synopsis In Situ Hybridization Methods by : Giselbert Hauptmann

Download or read book In Situ Hybridization Methods written by Giselbert Hauptmann and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

MiRNomics

Author : Malik Yousef
Publisher :
ISBN 13 : 9781627037488
Total Pages : 333 pages
Book Rating : 4.0/5 (374 download)

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Book Synopsis MiRNomics by : Malik Yousef

Download or read book MiRNomics written by Malik Yousef and published by . This book was released on 2014 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: In miRNomics: MicroRNA Biology and Computational Analysis, expert researchers in the field present an overview of the current state of the art and aim to put the respective areas of research into a larger perspective. These include methods and techniques ranging from miRNA biogenesis, their biological function, computational analyses to their medical implications and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNomics: MicroRNA Biology and Computational Analysis seeks to aid scientists in the further study into miRNA research and statistics.

Microrna

Author : James A. Mulligan
Publisher : Nova Science Publishers
ISBN 13 : 9781621001522
Total Pages : 299 pages
Book Rating : 4.0/5 (15 download)

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Book Synopsis Microrna by : James A. Mulligan

Download or read book Microrna written by James A. Mulligan and published by Nova Science Publishers. This book was released on 2011 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt:

MicroRNAs as Tools in Biopharmaceutical Production

Author : Niall Barron
Publisher : Springer Science & Business Media
ISBN 13 : 9400751281
Total Pages : 126 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis MicroRNAs as Tools in Biopharmaceutical Production by : Niall Barron

Download or read book MicroRNAs as Tools in Biopharmaceutical Production written by Niall Barron and published by Springer Science & Business Media. This book was released on 2012-11-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

MicroRNA Profiling

Author : Sweta Rani
Publisher : Springer Nature
ISBN 13 : 1071628232
Total Pages : 258 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis MicroRNA Profiling by : Sweta Rani

Download or read book MicroRNA Profiling written by Sweta Rani and published by Springer Nature. This book was released on 2022-11-28 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Nanotechnology-Enabled Sensors

Author : Kourosh Kalantar-zadeh
Publisher : Springer Science & Business Media
ISBN 13 : 0387680233
Total Pages : 502 pages
Book Rating : 4.3/5 (876 download)

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Book Synopsis Nanotechnology-Enabled Sensors by : Kourosh Kalantar-zadeh

Download or read book Nanotechnology-Enabled Sensors written by Kourosh Kalantar-zadeh and published by Springer Science & Business Media. This book was released on 2007-09-19 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology provides tools for creating functional materials, devices, and systems by controlling materials at the atomic and molecular scales and making use of novel properties and phenomena. Nanotechnology-enabled sensors find applications in several fields such as health and safety, medicine, process control and diagnostics. This book provides the reader with information on how nanotechnology enabled sensors are currently being used and how they will be used in the future in such diverse fields as communications, building and facilities, medicine, safety, and security, including both homeland defense and military operations.

RNA Detection

Author : Imre Gaspar
Publisher : Humana Press
ISBN 13 : 9781493972128
Total Pages : 492 pages
Book Rating : 4.9/5 (721 download)

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Book Synopsis RNA Detection by : Imre Gaspar

Download or read book RNA Detection written by Imre Gaspar and published by Humana Press. This book was released on 2017-11-15 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

MicroRNA Profiling in Cancer

Author : Yuriy Gusev
Publisher : CRC Press
ISBN 13 : 9814267546
Total Pages : 257 pages
Book Rating : 4.8/5 (142 download)

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Book Synopsis MicroRNA Profiling in Cancer by : Yuriy Gusev

Download or read book MicroRNA Profiling in Cancer written by Yuriy Gusev and published by CRC Press. This book was released on 2019-09-06 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents current advances in the emerging interdisciplinary field of microRNA research of human cancers from a unique perspective of quantitative sciences: bioinformatics, computational and systems biology, and mathematical modeling. This volume contains adaptations and critical reviews of recent state-of-the-art studies, ranging from technological advances in microRNA detection and profiling, clinically oriented microRNA profiling in several human cancers, to a systems biology analysis of global patterns of microRNA regulation of signaling and metabolic pathways. Interactions with transcription factor regulatory networks and mathematical modeling of microRNA regulation are also discussed.

RT-PCR Protocols

Author : Nicola King
Publisher : Springer Science & Business Media
ISBN 13 : 159259283X
Total Pages : 370 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis RT-PCR Protocols by : Nicola King

Download or read book RT-PCR Protocols written by Nicola King and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.

MicroRNA Methods

Author : John J. Rossi
Publisher : Academic Press
ISBN 13 : 9780123739179
Total Pages : 304 pages
Book Rating : 4.7/5 (391 download)

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Book Synopsis MicroRNA Methods by : John J. Rossi

Download or read book MicroRNA Methods written by John J. Rossi and published by Academic Press. This book was released on 2007-09-13 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.

Next-Generation MicroRNA Expression Profiling Technology

Author : Jian-Bing Fan
Publisher : Humana Press
ISBN 13 : 9781617794261
Total Pages : 341 pages
Book Rating : 4.7/5 (942 download)

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Book Synopsis Next-Generation MicroRNA Expression Profiling Technology by : Jian-Bing Fan

Download or read book Next-Generation MicroRNA Expression Profiling Technology written by Jian-Bing Fan and published by Humana Press. This book was released on 2011-12-08 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular BiologyTM series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

Biosensors

Author :
Publisher : BoD – Books on Demand
ISBN 13 : 1839624310
Total Pages : 256 pages
Book Rating : 4.8/5 (396 download)

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Book Synopsis Biosensors by :

Download or read book Biosensors written by and published by BoD – Books on Demand. This book was released on 2021-05-05 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers novel and current strategies for biosensing, from the use of nanomaterials and biological functionalized surfaces to the mathematical assessment of novel biosensors and their potential use as wearable devices for continuous monitoring. Biosensing technologies can be used in the medical field for the early detection of disease, monitoring effectiveness of treatments, detecting nervous system signals for controlling robotic prosthesis, and much more. This book includes eleven chapters that examine and discuss several strategies of biosensing, proposing mathematical designs that address the latest reported technologies.