Microfluidic Methods For Molecular Biology

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Microfluidic Methods for Molecular Biology

Author : Chang Lu
Publisher : Springer
ISBN 13 : 3319300199
Total Pages : 382 pages
Book Rating : 4.3/5 (193 download)

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Book Synopsis Microfluidic Methods for Molecular Biology by : Chang Lu

Download or read book Microfluidic Methods for Molecular Biology written by Chang Lu and published by Springer. This book was released on 2016-05-14 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Microfluidic Techniques

Author : Shelley D. Minteer
Publisher : Springer Science & Business Media
ISBN 13 : 1592599974
Total Pages : 245 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Microfluidic Techniques by : Shelley D. Minteer

Download or read book Microfluidic Techniques written by Shelley D. Minteer and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Microfluidic Diagnostics

Author : Gareth Jenkins
Publisher : Humana Press
ISBN 13 : 9781493962129
Total Pages : 540 pages
Book Rating : 4.9/5 (621 download)

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Book Synopsis Microfluidic Diagnostics by : Gareth Jenkins

Download or read book Microfluidic Diagnostics written by Gareth Jenkins and published by Humana Press. This book was released on 2016-05-01 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology book covers microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methods from basic fabrication methods to commercializing research. Includes materials, protocols, tips and more."

Microfluidics for Biologists

Author : Chandra K. Dixit
Publisher : Springer
ISBN 13 : 3319400363
Total Pages : 256 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Microfluidics for Biologists by : Chandra K. Dixit

Download or read book Microfluidics for Biologists written by Chandra K. Dixit and published by Springer. This book was released on 2016-10-13 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Author :
Publisher : Academic Press
ISBN 13 : 0128142855
Total Pages : 266 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis by :

Download or read book Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis written by and published by Academic Press. This book was released on 2018-11-22 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidics Diagnostics

Author : Valérie Taly
Publisher : Springer Nature
ISBN 13 : 1071638505
Total Pages : 254 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Microfluidics Diagnostics by : Valérie Taly

Download or read book Microfluidics Diagnostics written by Valérie Taly and published by Springer Nature. This book was released on with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

Author :
Publisher : Academic Press
ISBN 13 : 0128142839
Total Pages : 250 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Microfluidics in Cell Biology Part B: Microfluidics in Single Cells by :

Download or read book Microfluidics in Cell Biology Part B: Microfluidics in Single Cells written by and published by Academic Press. This book was released on 2018-08-27 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidics in Cell Biology: Part A: Microfluidics for Multicellular Systems

Author :
Publisher : Academic Press
ISBN 13 : 0128142812
Total Pages : 276 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Microfluidics in Cell Biology: Part A: Microfluidics for Multicellular Systems by :

Download or read book Microfluidics in Cell Biology: Part A: Microfluidics for Multicellular Systems written by and published by Academic Press. This book was released on 2018-07-20 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Single-Cell Assays

Author : Paul C.H. Li
Publisher : Springer Nature
ISBN 13 : 1071633236
Total Pages : 227 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Single-Cell Assays by : Paul C.H. Li

Download or read book Single-Cell Assays written by Paul C.H. Li and published by Springer Nature. This book was released on 2023-07-10 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores the use of single-cell assays in research for drug discovery, microfluidics, and more. The book delves into methodologies involving a variety of cell types and diseases, small molecules and biologics, as well as studies of the genome and transciptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Assays: Microfluidics, Genomics, and Drug Discovery serves to enable researchers to obtain a rapid overview in state-of-the-art microfluidic single-cell assays and an impression of what possibilities these assays offer to drug discovery.

Microchip Diagnostics

Author : Valérie Taly
Publisher : Humana
ISBN 13 : 9781493982868
Total Pages : 223 pages
Book Rating : 4.9/5 (828 download)

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Book Synopsis Microchip Diagnostics by : Valérie Taly

Download or read book Microchip Diagnostics written by Valérie Taly and published by Humana. This book was released on 2018-06-08 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. Microchip Diagnostics: Methods and Protocols is divided into four sections describing the business aspects of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cell Analysis on Microfluidics

Author : Jin-Ming Lin
Publisher : Springer
ISBN 13 : 9811053944
Total Pages : 435 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Cell Analysis on Microfluidics by : Jin-Ming Lin

Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Microfluidics for Biological Applications

Author : Wei-Cheng Tian
Publisher : Springer Science & Business Media
ISBN 13 : 0387094806
Total Pages : 429 pages
Book Rating : 4.3/5 (87 download)

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Book Synopsis Microfluidics for Biological Applications by : Wei-Cheng Tian

Download or read book Microfluidics for Biological Applications written by Wei-Cheng Tian and published by Springer Science & Business Media. This book was released on 2009-03-02 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics for Biological Applications provides researchers and scientists in the biotechnology, pharmaceutical, and life science industries with an introduction to the basics of microfluidics and also discusses how to link these technologies to various biological applications at the industrial and academic level. Readers will gain insight into a wide variety of biological applications for microfluidics. The material presented here is divided into four parts, Part I gives perspective on the history and development of microfluidic technologies, Part II presents overviews on how microfluidic systems have been used to study and manipulate specific classes of components, Part III focuses on specific biological applications of microfluidics: biodefense, diagnostics, high throughput screening, and tissue engineering and finally Part IV concludes with a discussion of emerging trends in the microfluidics field and the current challenges to the growth and continuing success of the field.

Biological Applications of Microfluidics

Author : Frank A. Gomez
Publisher : John Wiley & Sons
ISBN 13 : 0470074833
Total Pages : 562 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Biological Applications of Microfluidics by : Frank A. Gomez

Download or read book Biological Applications of Microfluidics written by Frank A. Gomez and published by John Wiley & Sons. This book was released on 2008-02-15 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics has numerous potential applications in biotechnology, pharmaceuticals, the life sciences, defense, public health, and agriculture. This book details recent advances in the biological applications of microfluidics, including cell sorting, DNA sequencing on-a-chip, microchip capillary electrophoresis, and synthesis on a microfluidic format. It covers microfabricated LOC technologies, advanced microfluidic tools, microfluidic culture platforms for stem cell and neuroscience research, and more. This is an all-in-one, hands-on resource for analytical chemists and researchers and an excellent text for students.

Microfluidic Electrophoresis

Author : Debashis Dutta
Publisher : Humana Press
ISBN 13 : 9781493989638
Total Pages : 255 pages
Book Rating : 4.9/5 (896 download)

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Book Synopsis Microfluidic Electrophoresis by : Debashis Dutta

Download or read book Microfluidic Electrophoresis written by Debashis Dutta and published by Humana Press. This book was released on 2018-11-29 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

Reinventing Microinjection

Author : Jan de Sonneville
Publisher :
ISBN 13 : 9789088913433
Total Pages : 135 pages
Book Rating : 4.9/5 (134 download)

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Book Synopsis Reinventing Microinjection by : Jan de Sonneville

Download or read book Reinventing Microinjection written by Jan de Sonneville and published by . This book was released on 2011 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microfluidics

Author : Sagnik Basuray
Publisher : Springer Science & Business Media
ISBN 13 : 3642230490
Total Pages : 352 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis Microfluidics by : Sagnik Basuray

Download or read book Microfluidics written by Sagnik Basuray and published by Springer Science & Business Media. This book was released on 2011-10-05 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow Control Methods and Devices in Micrometer Scale Channels, by Shuichi Shoji and Kentaro Kawai. Micromixing Within Microfluidic Devices, by Lorenzo Capretto, Wei Cheng, Martyn Hill and Xunli Zhang. Basic Technologies for Droplet Microfluidics, by Shaojiang Zeng, Xin Liu, Hua Xie and Bingcheng Lin. Electrorheological Fluid and Its Applications in Microfluidics, by Limu Wang, Xiuqing Gong and Weijia Wen. Biosensors in Microfluidic Chips, by Jongmin Noh, Hee Chan Kim and Taek Dong Chung. A Nanomembrane-Based Nucleic Acid Sensing Platform for Portable Diagnostics, by Satyajyoti Senapati, Sagnik Basuray, Zdenek Slouka, Li-Jing Cheng and Hsueh-Chia Chang. Optical Detection Systems on Microfluidic Chips, by Hongwei Gai, Yongjun Li and Edward S. Yeung. Integrated Microfluidic Systems for DNA Analysis, by Samuel K. Njoroge, Hui-Wen Chen, Małgorzata A. Witek and Steven A. Soper. Integrated Multifunctional Microfluidics for Automated Proteome Analyses, by John K. Osiri, Hamed Shadpour, Małgorzata A. Witek and Steven A. Soper. Cells in Microfluidics, by Chi Zhang and Danny van Noort. Microfluidic Platform for the Study of Caenorhabditis elegans,by Weiwei Shi, Hui Wen, Bingcheng Lin and Jianhua Qin.

Microengineering in Biotechnology

Author : Michael Pycraft Hughes
Publisher : Springer Nature
ISBN 13 :
Total Pages : 272 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Microengineering in Biotechnology by : Michael Pycraft Hughes

Download or read book Microengineering in Biotechnology written by Michael Pycraft Hughes and published by Springer Nature. This book was released on 2010 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microelectronic engineering has revolutionized electronics. This book instills in the reader a working understanding of the methods underlying microengineering and the means by which such methods can be used for a range of analytical techniques.