Microbial Community Analysis Assessed by Pyrosequencing of RRNA Gene

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Publisher :
ISBN 13 :
Total Pages : 348 pages
Book Rating : 4.3/5 (129 download)

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Book Synopsis Microbial Community Analysis Assessed by Pyrosequencing of RRNA Gene by : Woo Jun Sul

Download or read book Microbial Community Analysis Assessed by Pyrosequencing of RRNA Gene written by Woo Jun Sul and published by . This book was released on 2009 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Endodontic Microbiology

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Publisher : John Wiley & Sons
ISBN 13 : 1118975502
Total Pages : 878 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Endodontic Microbiology by : Ashraf F. Fouad

Download or read book Endodontic Microbiology written by Ashraf F. Fouad and published by John Wiley & Sons. This book was released on 2017-02-06 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endodontic Microbiology, Second Edition presents a comprehensive reference to the microbiology, pathogenesis, management, and healing of endodontic pathosis, emphasizing the importance of biological sciences in understanding and managing endodontic disease and its interaction with systemic health. Provides a major revision to the first book to focus on the problems related to microbes in the root canal and periapical tissues Updates current knowledge in endodontic pathosis, especially regarding next generation sequencing and microbial virulence Presents useful diagrams, images, radiographs, and annotated histological images to illustrate the concepts Emphasizes the importance of biological science in understanding and managing endodontic disease Includes contributions from the leading researchers and educators in the field

Molecular Microbial Ecology Manual

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Publisher : Springer
ISBN 13 : 9400902158
Total Pages : 266 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Molecular Microbial Ecology Manual by : Antoon D. L. Akkermans

Download or read book Molecular Microbial Ecology Manual written by Antoon D. L. Akkermans and published by Springer. This book was released on 2014-01-13 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.

16s RRNA-based Tag Pyrosequencing of Complex Food and Wastewater Environments

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (778 download)

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Book Synopsis 16s RRNA-based Tag Pyrosequencing of Complex Food and Wastewater Environments by : Katherine McElhany

Download or read book 16s RRNA-based Tag Pyrosequencing of Complex Food and Wastewater Environments written by Katherine McElhany and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Environmental microbiology has traditionally been performed using culture-based methods. However, in the last few decades, the emergence of molecular methods has changed the field considerably. The latest development in this area has been the introduction of next-generation sequencing, including pyrosequencing. These technologies allow the massively parallel sequencing of millions of DNA strands and represent a major development in sequencing technologies. The purpose of this study was to use both pyrosequencing and traditional culture-based techniques to investigate the diversity and dynamics of bacterial populations within milk and untreated sewage sludge samples. Pasteurized and raw milk samples were collected from grocery stores and dairies within Texas. Milk samples were analyzed by plating, pyrosequencing, and an assay for the presence of cell-cell signaling molecules. Samples were processed, stored, and then evaluated again for spoilage microflora. The results of this study showed that raw milk had a considerably higher bacterial load, more diversity between samples, and a significantly higher concentration of pathogens than pasteurized milk. Additionally, this study provided evidence for varying spoilage microflora between raw and pasteurized milk, as well as evidence for the production of cell-cell signaling molecules by bacterial organisms involved in milk spoilage. Four samplings of untreated sewage sludge were collected from wastewater treatment plants in seven different municipalities across the United States. Samples were subjected to quantification of selected bacterial organisms by culture and a pyrosequencing analysis was performed on extracted community DNA. The results of this study showed that untreated sewage sludge is inhabited by a huge diversity of microorganisms and that certain municipalities may have distinct bacterial populations that are conserved over time. Additionally, this study provided some evidence for seasonal differences in several of the major bacterial phyla. Lastly, this study emphasized the challenges of comparing results obtained by culture and pyrosequencing. In conclusion, this study showed that both milk and sewage are highly diverse, dynamic environments that can contain organisms of public health concern. The use of both culture-based methods and pyrosequencing in this study proved a complementary approach, providing a more comprehensive picture of both microbial environments.

Nonlinear Regression

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Publisher : John Wiley & Sons
ISBN 13 : 0471725307
Total Pages : 768 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Nonlinear Regression by : George A. F. Seber

Download or read book Nonlinear Regression written by George A. F. Seber and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: WILEY-INTERSCIENCE PAPERBACK SERIES The Wiley-Interscience Paperback Series consists of selected books that have been made more accessible to consumers in an effort to increase global appeal and general circulation. With these new unabridged softcover volumes, Wiley hopes to extend the lives of these works by making them available to future generations of statisticians, mathematicians, and scientists. From the Reviews of Nonlinear Regression "A very good book and an important one in that it is likely to become a standard reference for all interested in nonlinear regression; and I would imagine that any statistician concerned with nonlinear regression would want a copy on his shelves." –The Statistician "Nonlinear Regression also includes a reference list of over 700 entries. The compilation of this material and cross-referencing of it is one of the most valuable aspects of the book. Nonlinear Regression can provide the researcher unfamiliar with a particular specialty area of nonlinear regression an introduction to that area of nonlinear regression and access to the appropriate references . . . Nonlinear Regression provides by far the broadest discussion of nonlinear regression models currently available and will be a valuable addition to the library of anyone interested in understanding and using such models including the statistical researcher." –Mathematical Reviews

Microbial Environmental Genomics (MEG)

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Publisher : Springer Nature
ISBN 13 : 1071628712
Total Pages : 370 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Microbial Environmental Genomics (MEG) by : Francis Martin

Download or read book Microbial Environmental Genomics (MEG) written by Francis Martin and published by Springer Nature. This book was released on 2022-12-15 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume guides researchers on how to characterize, image rare, and hitherto unknown taxa and their interactions, to identify new functions and biomolecules and to understand how environmental changes condition the activity and the response of the organisms living with us and in our environment. Chapters cover different organism types (i.e., archaea, bacteria, fungi, protest, microfauna and microeukaryotes) and propose detailed protocols to produce high quality DNA, to analyse active microbial communities directly involved in complex interactions or processes through stable isotope probing, to identify and characterize of new functional genes, to image in situ interactions and to apply bioinformatics analysis tools to complex metagenomic or RNAseq sequence data. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial Environmental Genomics (MEG): Methods and Protocols, Second Edition aims to serve as a primary research reference for researchers in microbiology working to in the expanding field of molecular ecology and environmental genomics.

Metalliferous Sediments of the World Ocean

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Publisher : Springer Science & Business Media
ISBN 13 : 3540309691
Total Pages : 424 pages
Book Rating : 4.5/5 (43 download)

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Book Synopsis Metalliferous Sediments of the World Ocean by : Evgeny G. Gurvich

Download or read book Metalliferous Sediments of the World Ocean written by Evgeny G. Gurvich and published by Springer Science & Business Media. This book was released on 2006-01-16 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dramatic advances in understanding global tectonics have been made in the last half century and the information and specific data acquired on the floor of the World Ocean by the scientific community probably has exc- ded that available in all previous time. With the benefit of new technology and advanced concepts in the earth sciences extensive exploration of the deep seabed became possible, and has been carried out in many parts of the world. Many features have been recognized and data recorded that are vital for understanding the fundamental processes that shape the earth=s surface and control the habitable environment. The data collected to date on the o- an floor and its physical environment greatly exceeds our understanding and appreciation of their fundamental importance in the earth sciences, and our ability to apply this knowledge effectively in improving our way of life. With his extensive scientific knowledge and unique experience from - ny cruises in association with scientists throughout the world, Dr. Evgeny Gurvich has made an outstanding contribution in acquiring basic data on hydrothermal and sedimentation processes in the ocean, as well as in the synthesis of data and concepts available from cruise reports and an extensive literature.

Nucleic Acid Techniques in Bacterial Systematics

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ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Nucleic Acid Techniques in Bacterial Systematics by : Erko Stackebrandt

Download or read book Nucleic Acid Techniques in Bacterial Systematics written by Erko Stackebrandt and published by . This book was released on 1991-04-19 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of a series whose aim is to identify specialist areas in microbiology and to provide up-to-date methodological information for laboratory microbiologists, active researchers and graduate students. This volume addresses nucleic acid techniques in bacterial systematics.

Microbiomes of the Built Environment

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Publisher : National Academies Press
ISBN 13 : 0309449839
Total Pages : 318 pages
Book Rating : 4.3/5 (94 download)

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Book Synopsis Microbiomes of the Built Environment by : National Academies of Sciences, Engineering, and Medicine

Download or read book Microbiomes of the Built Environment written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2017-10-06 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: People's desire to understand the environments in which they live is a natural one. People spend most of their time in spaces and structures designed, built, and managed by humans, and it is estimated that people in developed countries now spend 90 percent of their lives indoors. As people move from homes to workplaces, traveling in cars and on transit systems, microorganisms are continually with and around them. The human-associated microbes that are shed, along with the human behaviors that affect their transport and removal, make significant contributions to the diversity of the indoor microbiome. The characteristics of "healthy" indoor environments cannot yet be defined, nor do microbial, clinical, and building researchers yet understand how to modify features of indoor environmentsâ€"such as building ventilation systems and the chemistry of building materialsâ€"in ways that would have predictable impacts on microbial communities to promote health and prevent disease. The factors that affect the environments within buildings, the ways in which building characteristics influence the composition and function of indoor microbial communities, and the ways in which these microbial communities relate to human health and well-being are extraordinarily complex and can be explored only as a dynamic, interconnected ecosystem by engaging the fields of microbial biology and ecology, chemistry, building science, and human physiology. This report reviews what is known about the intersection of these disciplines, and how new tools may facilitate advances in understanding the ecosystem of built environments, indoor microbiomes, and effects on human health and well-being. It offers a research agenda to generate the information needed so that stakeholders with an interest in understanding the impacts of built environments will be able to make more informed decisions.

Microbes and Microbial Technology

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Publisher : Springer Science & Business Media
ISBN 13 : 144197931X
Total Pages : 522 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Microbes and Microbial Technology by : Iqbal Ahmad

Download or read book Microbes and Microbial Technology written by Iqbal Ahmad and published by Springer Science & Business Media. This book was released on 2011-02-01 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on successful application of microbial biotechnology in areas such as medicine, agriculture, environment and human health.

Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa

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Publisher : CRC Press
ISBN 13 : 135109081X
Total Pages : 177 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa by : B.A. Dehority

Download or read book Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa written by B.A. Dehority and published by CRC Press. This book was released on 2018-01-18 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: The only rumen protozoa lab guide featuring line drawings created by a leading scientist in the field Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa is a unique lab guide for learning how to count and identify rumen protozoa. In this guide, Professor Dehority has created line drawings of rumen protozoa that emphasize morphological features and size measurements. The book also provides keys for identifying genera and species, and it contains classifications and descriptions of the different orders and families of rumen ciliate protozoa. Procedures for counting rumen protozoa and identifying individual species are included as well. Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa will be an excellent identification guide for protozoologists, microbiologists, dairy scientists, and any researcher or student working with rumen protozoa.

Using Genomics, Metagenomics and Other "Omics" to Assess Valuable Microbial Ecosystem Services and Novel Biotechnological Applications

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Publisher : Frontiers Media SA
ISBN 13 : 2889458148
Total Pages : 674 pages
Book Rating : 4.8/5 (894 download)

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Book Synopsis Using Genomics, Metagenomics and Other "Omics" to Assess Valuable Microbial Ecosystem Services and Novel Biotechnological Applications by : Diana Elizabeth Marco

Download or read book Using Genomics, Metagenomics and Other "Omics" to Assess Valuable Microbial Ecosystem Services and Novel Biotechnological Applications written by Diana Elizabeth Marco and published by Frontiers Media SA. This book was released on 2019-05-15 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most ecosystem services and goods human populations use and consume are provided by microbial populations and communities. Indeed, numerous provisioning services (e.g. food and enzymes for industrial processes), regulating services (e.g. water quality, contamination alleviation and biological processes such as plant-microbial symbioses), and supporting services (e.g. nutrient cycling, agricultural production and biodiversity) are mediated by microbes. The fast development of metagenomics and other meta-omics technologies is expanding our understanding of microbial diversity, ecology, evolution and functioning. This enhanced knowledge directly translates into the emergence of new applications in an unlimited variety of areas across all microbial ecosystem services and goods. The varied topics addressed in this Research Topic include the development of innovative industrial processes, the discovery of novel natural products, the advancement of new agricultural methods, the amelioration of negative effects of productive or natural microbiological processes, as well as food security and human health, and archeological conservation. The articles compiled provide an updated, high-quality overview of current work in the field. This body of research makes a valuable contribution to the understanding of microbial ecosystem services, and expands the horizon for finding and developing new and more efficient biotechnological applications.

The New Science of Metagenomics

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Publisher : National Academies Press
ISBN 13 : 0309106761
Total Pages : 170 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis The New Science of Metagenomics by : National Research Council

Download or read book The New Science of Metagenomics written by National Research Council and published by National Academies Press. This book was released on 2007-06-24 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although we can't usually see them, microbes are essential for every part of human life-indeed all life on Earth. The emerging field of metagenomics offers a new way of exploring the microbial world that will transform modern microbiology and lead to practical applications in medicine, agriculture, alternative energy, environmental remediation, and many others areas. Metagenomics allows researchers to look at the genomes of all of the microbes in an environment at once, providing a "meta" view of the whole microbial community and the complex interactions within it. It's a quantum leap beyond traditional research techniques that rely on studying-one at a time-the few microbes that can be grown in the laboratory. At the request of the National Science Foundation, five Institutes of the National Institutes of Health, and the Department of Energy, the National Research Council organized a committee to address the current state of metagenomics and identify obstacles current researchers are facing in order to determine how to best support the field and encourage its success. The New Science of Metagenomics recommends the establishment of a "Global Metagenomics Initiative" comprising a small number of large-scale metagenomics projects as well as many medium- and small-scale projects to advance the technology and develop the standard practices needed to advance the field. The report also addresses database needs, methodological challenges, and the importance of interdisciplinary collaboration in supporting this new field.

Encyclopedia of Metagenomics

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Publisher : Springer
ISBN 13 : 9781461446743
Total Pages : 1528 pages
Book Rating : 4.4/5 (467 download)

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Book Synopsis Encyclopedia of Metagenomics by : Karen E. Nelson

Download or read book Encyclopedia of Metagenomics written by Karen E. Nelson and published by Springer. This book was released on 2015-02-18 with total page 1528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metagenomics has taken off as one of the major cutting-edge fields of research. The field has broad implications for human health and disease, animal production, and environmental health. Metagenomics has opened up a wealth of data, tools, technologies and applications that allow us to access the majority of organisms that we still cannot access in pure culture (an estimated 99% of microbial life). Numerous research groups are developing tools, approaches and applications to deal with this new field, as larger data sets from environments including the human body, the oceans and soils are being generated. See for example the human microbiome initiative (HMP; http://nihroadmap.nih.gov/hmp/) which has become a world-wide effort, and the Global Ocean Sampling (GOS) surveys; http://www.jcvi.org/cms/research/projects/gos/overview/). The number of publications as measured through PubMed that are focused on metagenomics continues to increase. The field of metagenomics continues to evolve with large common datasets available to the scientific community. A concerted effort is needed to collate all this information in a centralized place. By having all the information in an Encyclopedia form, we have an opportunity to gather seminal contributions from the leaders in the field, and at the same time provide this information to a significant number of junior and senior scientists. It is anticipated that the Encyclopedia will also be used by many other groups including, clinicians, undergraduate and graduate level students, as well as ethical and legal groups associated with or interested in the issues surrounding metagenome science.

Criminal and Environmental Soil Forensics

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Publisher : Springer Science & Business Media
ISBN 13 : 1402092040
Total Pages : 534 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Criminal and Environmental Soil Forensics by : Karl Ritz

Download or read book Criminal and Environmental Soil Forensics written by Karl Ritz and published by Springer Science & Business Media. This book was released on 2008-12-23 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soils have important roles to play in criminal and environmental forensic science. Since the initial concept of using soil in forensic investigations was mooted by Conan Doyle in his Sherlock Holmes stories prior to real-world applications, this branch of forensic science has become increasingly sophisticated and broad. New techniques in chemical, physical, biological, ecological and spatial analysis, coupled with informatics, are being applied to reducing areas of search by investigators, site identification, site comparison and measurement for the eventual use as evidence in court. Soils can provide intelligence, in assisting the determination of the provenance of samples from artifacts, victims or suspects, enabling their linkage to locations or other evidence. They also modulate change in surface or buried cadavers and hence affect the ability to estimate post-mortem or post-burial intervals, and locate clandestine graves. This interdisciplinary volume explores the conceptual and practical interplay of soil and geoforensics across the scientific, investigative and legal fields. Supported by reviews, case-studies from across the world, and reports of original research, it demonstrates the increasing convergence of a wide range of knowledge. It covers conceptual issues, evidence (from recovery to use in court), geoforensics, taphonomy, as well as leading-edge technologies. The application of the resultant soil forensics toolbox is leading to significant advances in improving crime detection, and environmental and national security.

Microbial Communities in Coastal Sediments

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Publisher : Elsevier
ISBN 13 : 0128151668
Total Pages : 206 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Microbial Communities in Coastal Sediments by : Salom Gnana Thanga Vincent

Download or read book Microbial Communities in Coastal Sediments written by Salom Gnana Thanga Vincent and published by Elsevier. This book was released on 2021-06-02 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise reference on the structural composition and function of microbial communities in coastal environments, especially in relation to natural and anthropogenic impacts. Microbial Communities in Coastal Sediments presents twenty years of coastal microbiology research, grounding it as a vital development in the field of microbial ecology. It is the first book to focus exclusively on the complex microbial ecology and its function in rest of the marine environment. The book outlines the structure, function, and assessment of microbial communities in marine sediments while exploring practical methods of assessment. It is an invaluable resource to aquatic microbiologists, marine ecologists, marine microbiologists, aquatic researchers, and graduate students in this field. Microbial Communities in Coastal Sediments begins with an examination of nutrient sources in the coastal context with a focus on organic matter inputs. The quantity and quality of organic matter in coastal sediments and their impacts on the composition and formation of microbial communities is discussed. The book explores the consequences of anthropogenic changes and human activity on microbial ecology and nutrient cycling. Sections on nutrient availability, green house gas production and biodegradation of persistent organic pollutants provide essential details. Molecular research techniques and methods for assessing microbial community structure and function in coastal sediments are also covered. Explores the interplay of physicochemical and biological features of coastal ecosystems on microbial community composition to provide a template of comparison for field research Includes unique figures, schematic diagrams and photographs related to microbial processes of coastal ecosystem to clearly represent different aspects of microbial structure and functions Provides analytical methods and detailed molecular techniques for qualitative and quantitative analyses of microbial community structure

Psychrophiles: From Biodiversity to Biotechnology

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Publisher : Springer
ISBN 13 : 3319570579
Total Pages : 685 pages
Book Rating : 4.3/5 (195 download)

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Book Synopsis Psychrophiles: From Biodiversity to Biotechnology by : Rosa Margesin

Download or read book Psychrophiles: From Biodiversity to Biotechnology written by Rosa Margesin and published by Springer. This book was released on 2017-06-22 with total page 685 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cold adaptation includes a complex range of structural and functional adaptations at the level of all cellular constituents, and these adaptations render cold-adapted organisms particularly useful for biotechnological applications. This book presents the most recent knowledge of (i) boundary conditions for microbial life in the cold, (ii) microbial diversity in various cold ecosystems, (iii) molecular cold adaptation mechanisms and (iv) the resulting biotechnological perspectives.