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Messenger Rna Polya Tail Metabolism In Saccharomyces Cerevisiae
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Book Synopsis Messenger RNA Poly(A) Tail Metabolism in Saccharomyces Cerevisiae by : Christine Elizabeth Brown
Download or read book Messenger RNA Poly(A) Tail Metabolism in Saccharomyces Cerevisiae written by Christine Elizabeth Brown and published by . This book was released on 1998 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Messenger RNA and Protein Metabolism During Sporulation of Saccharomyces Cerevisiae by : Ellen B. Kraig
Download or read book Messenger RNA and Protein Metabolism During Sporulation of Saccharomyces Cerevisiae written by Ellen B. Kraig and published by . This book was released on 1981 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis 3' Processing of Messenger RNA in the Yeast Saccharomyces Cerevisiae by : Christoph Mathias Egli
Download or read book 3' Processing of Messenger RNA in the Yeast Saccharomyces Cerevisiae written by Christoph Mathias Egli and published by . This book was released on 1995 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Role of RNA-binding Proteins in MRNA Metabolism in Saccharomyces Cerevisiae by : Elisa Chao-Fong Shen
Download or read book The Role of RNA-binding Proteins in MRNA Metabolism in Saccharomyces Cerevisiae written by Elisa Chao-Fong Shen and published by . This book was released on 2000 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis mRNA Metabolism & Post-Transcriptional Gene Regulation by : Joe B. Harford
Download or read book mRNA Metabolism & Post-Transcriptional Gene Regulation written by Joe B. Harford and published by John Wiley & Sons. This book was released on 1997-03-28 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: mRNA METABOLISM & POST-TRANSCRIPTIONAL GENE REGULATION Edited by Joe B. Harford and David R. Morris Gene expression is a process that begins with the transcription ofDNA to an RNA messenger (mRNA), which is then translated into aprotein. Historically, attention has been focused on the regulationof RNA synthesis (transcription); however, there is a growingrecognition of and appreciation for the importance of the manyregulatory mechanisms that take place after RNA synthesis has beencompleted. mRNA Metabolism and Post-Transcriptional Gene Regulation is thefirst comprehensive overview of the various modes of generegulation that exist post-transcriptionally. Collecting studies bysome of the top researchers in the field, this volume provides bothan up-to-date review of the complex "life" of an mRNA molecule andan introduction to current work on the diversity of mechanisms ofpost-transcriptional reactions. Topics covered include: * RNA structure * Mammalian RNA editing * RNA export from the nucleus * The fundamentals of translation initiation * Control of mRNA decay in plants * mRNA metabolism and cancer * Control of mRNA stability during herpes simplex virus infection * Regulation of mRNA expression in HIV-1 and other complexretroviruses * Nucleases * RNA localization A timely contribution to the understanding of genetic regulatorymechanisms, mRNA Metabolism and Post-Transcriptional GeneRegulation provides a basis from which potential therapeuticstrategies may be developed. This book will be of vital interest tocell and molecular biologists at all levels, from graduate studentsto senior investigators, clinical researchers, and professionals inthe pharmaceutical and biotechnology industries.
Book Synopsis Control of MRNA Metabolism by Poly(A) Binding Protein and Development of the Tethered Function Assay by : Jeffery Michael Coller
Download or read book Control of MRNA Metabolism by Poly(A) Binding Protein and Development of the Tethered Function Assay written by Jeffery Michael Coller and published by . This book was released on 2000 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Control of Gene Expression by Nuclear RNA Degradation in Saccharomyces Cerevisiae by : Kevin Richard Jones Roy
Download or read book The Control of Gene Expression by Nuclear RNA Degradation in Saccharomyces Cerevisiae written by Kevin Richard Jones Roy and published by . This book was released on 2015 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribonucleases play critical roles in controlling the quantity and quality of gene expression through processing and degrading RNA. An important class of evolutionarily conserved ribonucleases is the RNase III family of enzymes, which are distinguished by their specificity for cleaving double-stranded RNA (dsRNA). RNase III enzymes perform diverse functions in RNA metabolism in all eukaryotes studied, yet numerous questions remain regarding their range of natural targets in vivo, how they achieve substrate specificity, and how their cleavage activity is regulated. The model eukaryote Saccharomyces cerevisiae harbors one RNase III homolog, Rnt1p, which is responsible for all known dsRNA cleavage activity in this organism. To better understand the substrate selectivity of Rnt1p, we examined how its double-stranded RNA binding domain (dsRBD) recognizes a non-canonical substrate containing an AAGU tetraloop sequence differing from the NGNN consensus sequence. Surprisingly, we found that upon engaging the RNA, the dsRBD induces a structural change in the AAGU loop so that it closely adopts the structure of the NGNN loop. This suggested that the structures of isolated RNAs in solution are not necessarily predictive of substrate specificity. We next characterized how structural dynamics in the dsRBD mediate specific binding. We found that in order to bind substrate dsRNA with high affinity, the dsRBD must undergo a significant conformational change involving the first alpha helix and beta strand of the dsRBD. Next we implemented computational RNA secondary structure screens to scan the genome for potential Rnt1p targets. We identified a characteristic Rnt1p stem-loop in the BDF2 mRNA, which is also subject to nuclear decay by the spliceosome through a first step splicing discard pathway. Cis acting mutations in BDF2 blocking Rnt1p or spliceosome-mediated decay (SMD) conferred distinct phenotypes for each pathway, revealing that salt stress hyper-activates Rnt1p cleavage while spliceosome-mediated decay controls BDF2 expression during DNA replication stress. To globally identify RNA targets of Rnt1p cleavage, we leveraged the fact that the 5 product of Rnt1p cleavage is oligo-adenylated by Trf4/5-Air2/1-Mtr4 polyadenylation (TRAMP) complex prior to degradation by the nuclear exosome, a 3 -to-5 exonuclease complex. We mapped TRAMP poly(A) tails genome-wide by high-throughput sequencing of 3 ends of polyadenylated RNA in yeast cells lacking a nuclear exosome component. This revealed a global profile of destabilized 3 ends arising from various nuclear RNA degradation mechanisms, including Rnt1p cleavage, transcription termination by the Nrd1p-Nab3p-Sen1p (NNS) pathway and roadblock transcription termination by Reb1p and TFIIIB DNA binding factors. While the NNS pathway was known to play a prominent role in limiting pervasive RNA polymerase II, we uncovered previously unappreciated roles for roadblocks and Rnt1p in controlling Pol II transcriptional output throughout the genome, revealing how cells use a multitude of nuclear mechanisms to regulate the levels of coding and cryptic transcripts.
Book Synopsis mRNA Processing and Metabolism by : Daniel R. Schoenberg
Download or read book mRNA Processing and Metabolism written by Daniel R. Schoenberg and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of splicing factors, where the majority of pre-mRNAs undergo alternative splicing, and where proteins deposited during nuclear processing impact distal cytoplasmic processing, translation, and decay. If there is a unifying theme to mRNA Processing and Metabolism: Methods and Protocols, it is that mRNA processing and metabolism are integrated processes. Many of the techniques used to study mRNA have been described in a previous volume of this series (RNA–Protein Interaction Protocols, Susan Haynes, ed.) and specialized methods journals. In selecting topics for mRNA Processing and Metabolism: Methods and Protocols, I sought input on new and novel techniques and approaches that build on this foundation using technological advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference. I have tried not to bias this book toward any single model organism, and approaches described in the various chapters use yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells.
Book Synopsis Translation of Polyriboadenylic [Poly(A)] - Enriched Messenger RNA's from the Yeast, Saccharomyces Cerevisiae, in Homologous and Heterologous Cell-free Systems by : Byron M. Gallis
Download or read book Translation of Polyriboadenylic [Poly(A)] - Enriched Messenger RNA's from the Yeast, Saccharomyces Cerevisiae, in Homologous and Heterologous Cell-free Systems written by Byron M. Gallis and published by . This book was released on 1974 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of Metabolism by Transfer RNA in Saccharomyces Cerevisiae by : John Michael Zaborske (Jr)
Download or read book Regulation of Metabolism by Transfer RNA in Saccharomyces Cerevisiae written by John Michael Zaborske (Jr) and published by . This book was released on 2010 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transfer RNA has long been known to be essential for protein synthesis. Only relatively recently has its role as signal molecule been examined. Of particular interest is the role of uncharged tRNA in cellular regulation, specifically metabolism. Changes in tRNA charging, or aminoacylation, can occur very rapidly making it an ideal method for intracellular signaling.
Book Synopsis Translational Control of Gene Expression by : Nahum Sonenberg
Download or read book Translational Control of Gene Expression written by Nahum Sonenberg and published by CSHL Press. This book was released on 2001 with total page 1034 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control.
Book Synopsis A Study of RNA Metabolism in Saccharomyces Cerevisiae by : Kenneth Willard Boehlke
Download or read book A Study of RNA Metabolism in Saccharomyces Cerevisiae written by Kenneth Willard Boehlke and published by . This book was released on 1972 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of MRNA Decay in Saccharomyces Cerevisiae by the Sequence-specific RNA-binding Protein Vts1 by : Laura Marie Rendl
Download or read book Regulation of MRNA Decay in Saccharomyces Cerevisiae by the Sequence-specific RNA-binding Protein Vts1 written by Laura Marie Rendl and published by . This book was released on 2009 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vts1 is a member of the Smaug protein family, a group of sequence-specific RNA-binding proteins that regulate mRNA translation and degradation by binding to consensus stem-loop structures in target mRNAs. Using RNA reporters that recapitulate Vts1-mediated decay in vivo as well as endogenous mRNA transcripts, I show that Vts1 regulates the degradation of target mRNAs in Saccharomyces cerevisiae. In Chapter Two, I focus on the mechanism of Vts1-mediated mRNA decay. I demonstrate that Vts1 initiates mRNA degradation through deadenylation mediated by the Ccr4-Pop2-Not deadenylase complex. I also show that Vts1 interacts with the Ccr4-Pop2-Not deadenylase complex suggesting that Vts1 recruits the deadenylase machinery to target mRNAs, resulting in transcript decay. Following poly(A) tail removal, Vts1 target transcripts are decapped and subsequently degraded by the 5'-to-3' exonuclease Xrn1. Taken together these data suggest a mechanism of mRNA degradation that involves recruitment of the Ccr4-Pop2-Not deadenylase to target mRNAs. Previous work in Drosophila melanogaster demonstrated that Smg interacts with the Ccr4-Pop2-Not complex to regulate mRNA stability, suggesting Smaug family members employ a conserved mechanism of mRNA decay.In Drosophila, Smg also regulates mRNA translation through a separate mechanism involving the eIF4E-binding protein Cup. In Chapter Three, I identify the eIF4E-associated protein Eap1 as a component of Vts1-mediated mRNA decay in yeast. Interestingly Cup and Eap1 share no significant homology outside of the seven amino acid eIF4E-binding motif. In eap1Delta cells mRNAs accumulate as deadenylated capped species, suggesting that Eap1 stimulates mRNA decapping. I demonstrate that the Eap1 eIF4E-binding motif is required for efficient degradation of Vts1 target mRNAs and that this motif enables Eap1 to mediate an interaction between Vts1 and eIF4E. Together these data suggest Vts1 influences multiple steps in the mRNA decay pathway through interactions with the Ccr4-Pop2-Not deadenylase and the decapping activator Eap1.
Book Synopsis Control of Messenger RNA Stability by : Joel G. Belasco
Download or read book Control of Messenger RNA Stability written by Joel G. Belasco and published by Elsevier. This book was released on 2012-12-02 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first comprehensive review of mRNA stability and its implications for regulation of gene expression. Written by experts in the field, Control of Messenger RNA Stability serves both as a reference for specialists in regulation of mRNA stability and as a general introduction for a broader community of scientists. Provides perspectives from both prokaryotic and eukaryotic systems Offers a timely, comprehensive review of mRNA degradation, its regulation, and its significance in the control of gene expression Discusses the mechanisms, RNA structural determinants, and cellular factors that control mRNA degradation Evaluates experimental procedures for studying mRNA degradation
Book Synopsis Formation of Messenger RNA 3'ends in the Yeast “Saccharomyces Cerevisiae” by : Stefan Irniger
Download or read book Formation of Messenger RNA 3'ends in the Yeast “Saccharomyces Cerevisiae” written by Stefan Irniger and published by . This book was released on 1992 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA Metabolism in Mitochondria by : Jorge Cruz-Reyes
Download or read book RNA Metabolism in Mitochondria written by Jorge Cruz-Reyes and published by Springer. This book was released on 2018-06-12 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on mitochondrial RNA metabolism, emphasizing recent discoveries and technological advances in this fast moving area that increase our understanding of mitochondrial gene function. Topics addressed include the interplay of mitochondria with the nucleus and cytosol, structure-function connections, and relevance to human disease. Mitochondria are the powerhouses of the cell, and a great deal is known about mitochondrial energy metabolism. Less well known is the plethora of amazing mechanisms that have evolved to control expression of mitochondrial genomes. Several RNA processes and machineries in protozoa, plants, flies and humans are discussed, including: transcription and RNA polymerase mechanism; tRNA processing of 5′ and 3′ ends; mRNA maturation by nucleotide insertion/deletion editing and by RNA splicing; mRNA stability; and RNA import. Specialized factors and ribonucleoproteins (RNPs) examined include pentatricopeptide repeat (PPR) proteins, RNase P, polymerases, helicases, nucleases, editing and repair enzymes. Remarkable features of these processes and factors are either not found outside mitochondria, differ substantially among eukaryotic lineages, or are unique in biology.
Book Synopsis RNA Metabolism in Neurodegenerative Diseases by : Rita Sattler
Download or read book RNA Metabolism in Neurodegenerative Diseases written by Rita Sattler and published by Springer. This book was released on 2018-06-18 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has become evident over the last years that abnormalities in RNA processing play a fundamental part in the pathogenesis of neurodegenerative diseases. Cellular viability depends on proper regulation of RNA metabolism and subsequent protein synthesis, which requires the interplay of many processes including transcription, pre--‐mRNA splicing, mRNA editing as well as mRNA stability, transport and translation. Dysfunction in any of these processes, often caused by mutations in the coding and non--‐ coding RNAs, can be very destructive to the cellular environment and consequently impair neural viability. The result of this RNA toxicity can lead to a toxic gain of function or a loss of function, depending on the nature of the mutation. For example, in repeat expansion disorders, such as the newly discovered hexanucleotide repeat expansion in theC9orf72 gene found in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), a toxic gain of function leads to the formation of RNA foci and the sequestration of RNA binding proteins (RBPs). This in return leads to a loss of function of those RBPs, which is hypothesized to play a significant part in the disease progression of ALS and FTD. Other toxicities arising from repeat expansions are the formation of RNA foci, bi--‐directional transcription and production of repeat associated non--‐ATG (RAN) translation products. This book will touch upon most of these disease mechanisms triggered by aberrant RNA metabolism and will therefore provide a broad perspective of the role of RNA processing and its dysfunction in a variety of neurodegenerative disorders, including ALS, FTD, Alzheimer’s disease, Huntington’s disease, spinal muscular atrophy, myotonic dystrophy and ataxias. The proposed authors are leading scientists in the field and are expected to not only discuss their own work, but to be inclusive of historic as well as late breaking discoveries. The compiled chapters will therefore provide a unique collection of novel studies and hypotheses aimed to describe the consequences of altered RNA processing events and its newest molecular players and pathways.