Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics

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Publisher : John Wiley & Sons
ISBN 13 : 0470258861
Total Pages : 325 pages
Book Rating : 4.4/5 (72 download)

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Book Synopsis Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics by : M. Chance

Download or read book Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics written by M. Chance and published by John Wiley & Sons. This book was released on 2008-09-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Includes footprinting and plistex specifically, setting this book apart from the competition.

Mass Spectrometry of Protein Interactions

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Publisher : John Wiley & Sons
ISBN 13 : 047014632X
Total Pages : 153 pages
Book Rating : 4.4/5 (71 download)

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Book Synopsis Mass Spectrometry of Protein Interactions by : Kevin Downard

Download or read book Mass Spectrometry of Protein Interactions written by Kevin Downard and published by John Wiley & Sons. This book was released on 2007-08-24 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authoritative guide to analyzing protein interactions by mass spectrometry Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text: * Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS) * Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS) * Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions * Reviews the latest discoveries and applications and addresses new and ongoing challenges This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.

Mass Spectrometry of Protein Interactions

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Publisher :
ISBN 13 :
Total Pages : 420 pages
Book Rating : 4.:/5 (468 download)

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Book Synopsis Mass Spectrometry of Protein Interactions by : Adam A. Rostom

Download or read book Mass Spectrometry of Protein Interactions written by Adam A. Rostom and published by . This book was released on 1999 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein-Protein Interactions

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Author :
Publisher : Springer Nature
ISBN 13 : 1071633279
Total Pages : 480 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Protein-Protein Interactions by : Shahid Mukhtar

Download or read book Protein-Protein Interactions written by Shahid Mukhtar and published by Springer Nature. This book was released on 2023-07-14 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein-Protein Interactions: Methods and Protocols serves as an ideal guide for researchers working in protein science and beyond.

Protein Interactions

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Publisher : Springer Science & Business Media
ISBN 13 : 0387359664
Total Pages : 537 pages
Book Rating : 4.3/5 (873 download)

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Book Synopsis Protein Interactions by : Peter Schuck

Download or read book Protein Interactions written by Peter Schuck and published by Springer Science & Business Media. This book was released on 2007-03-20 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.

Proteomics and Protein-Protein Interactions

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Publisher : Springer Science & Business Media
ISBN 13 : 0387245324
Total Pages : 325 pages
Book Rating : 4.3/5 (872 download)

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Book Synopsis Proteomics and Protein-Protein Interactions by : Gabriel Waksman

Download or read book Proteomics and Protein-Protein Interactions written by Gabriel Waksman and published by Springer Science & Business Media. This book was released on 2006-12-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gabriel Waksman Institute of Structural Molecular Biology, Birkbeck and University College London, Malet Street, London WC1E 7HX, United Kingdom Address for correspondence: Professor Gabriel Waksman Institute of Structural Molecular Biology Birkbeck and University College London Malet Street London WC1E 7H United Kingdom Email: g. waksman@bbk. ac. uk and g. waksman@ucl. ac. uk Phone: (+44) (0) 207 631 6833 Fax: (+44) (0) 207 631 6833 URL: http://people. cryst. bbk. ac. uk/?ubcg54a Gabriel Waksman is Professor of Structural Molecular Biology at the Institute of Structural Molecular Biology at UCL/Birkbeck, of which he is also the director. Before joining the faculty of UCL and Birkbeck, he was the Roy and Diana Vagelos Professor of Biochemistry and Molecular Biophysics at the Washington University School of Medicine in St Louis (USA). The rapidly evolving ?eld of protein science has now come to realize the ubiquity and importance of protein–protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of hi- throughput proteomics to monitor protein–protein interactions at an organism level, we can now safely state that protein–protein interactions are the norm and not the exception.

Probing Protein-protein Interactions by Mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 147 pages
Book Rating : 4.:/5 (73 download)

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Book Synopsis Probing Protein-protein Interactions by Mass Spectrometry by : Tatiana Pimenova

Download or read book Probing Protein-protein Interactions by Mass Spectrometry written by Tatiana Pimenova and published by . This book was released on 2009 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein-protein Interactions and Networks

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Publisher : Springer Science & Business Media
ISBN 13 : 1848001258
Total Pages : 198 pages
Book Rating : 4.8/5 (48 download)

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Book Synopsis Protein-protein Interactions and Networks by : Anna Panchenko

Download or read book Protein-protein Interactions and Networks written by Anna Panchenko and published by Springer Science & Business Media. This book was released on 2010-04-06 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biological interactions of living organisms, and protein-protein interactions in particular, are astonishingly diverse. This comprehensive book provides a broad, thorough and multidisciplinary coverage of its field. It integrates different approaches from bioinformatics, biochemistry, computational analysis and systems biology to offer the reader a comprehensive global view of the diverse data on protein-protein interactions and protein interaction networks.

Characterization of Peptide Antigens and Protein-protein Interactions by Mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 244 pages
Book Rating : 4.X/5 (4 download)

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Book Synopsis Characterization of Peptide Antigens and Protein-protein Interactions by Mass Spectrometry by : Jennifer Nina Sutton

Download or read book Characterization of Peptide Antigens and Protein-protein Interactions by Mass Spectrometry written by Jennifer Nina Sutton and published by . This book was released on 2003 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Lipid-Protein Interactions

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Publisher : Humana
ISBN 13 : 9781493995110
Total Pages : 0 pages
Book Rating : 4.9/5 (951 download)

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Book Synopsis Lipid-Protein Interactions by : Jörg H. Kleinschmidt

Download or read book Lipid-Protein Interactions written by Jörg H. Kleinschmidt and published by Humana. This book was released on 2019-06-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

Protein-Protein Interactions

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Publisher : BoD – Books on Demand
ISBN 13 : 9535103970
Total Pages : 488 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Protein-Protein Interactions by : Weibo Cai

Download or read book Protein-Protein Interactions written by Weibo Cai and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.

Protein-Protein Interaction Assays

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Publisher : BoD – Books on Demand
ISBN 13 : 1789233909
Total Pages : 91 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Protein-Protein Interaction Assays by : Mahmood-Ur-Rahman Ansari

Download or read book Protein-Protein Interaction Assays written by Mahmood-Ur-Rahman Ansari and published by BoD – Books on Demand. This book was released on 2018-07-18 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: The chapters in this book are written by a team of well-reputed international researchers. The objective is to provide advanced and updated information related to protein-protein interactions. I hope the methods, resources and approaches described here will enhance the available knowledge of the reader significantly.

Characterization of Protein Interactions by Mass Spectrometry and Bioinformatics

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (113 download)

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Book Synopsis Characterization of Protein Interactions by Mass Spectrometry and Bioinformatics by : Victor Solis Mezarino

Download or read book Characterization of Protein Interactions by Mass Spectrometry and Bioinformatics written by Victor Solis Mezarino and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

New Mass Spectrometry Approaches to Investigate Proteins and Protein Interactions

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Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (134 download)

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Book Synopsis New Mass Spectrometry Approaches to Investigate Proteins and Protein Interactions by : Daniele Canzani

Download or read book New Mass Spectrometry Approaches to Investigate Proteins and Protein Interactions written by Daniele Canzani and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) is essential for understanding the composition, structures, and interactions of proteins. There are various forms of MS that can be used to obtain valuable information about proteins and protein interactions. In native MS, intact protein ions are generated from a solution that maintains the non-covalent protein interactions into the gas-phase environment of a mass spectrometer. MS is frequently coupled liquid chromatography (LC), which is an analytical technique that is used to separate proteins or peptides before they enter the mass spectrometer. LC can improve the sensitivity of an MS analysis and increase the amount of information that can be obtained from a sample. Ion mobility (IM) is a gas-phase separation technique that occurs after a protein sample has been ionized. IM-MS measurements can provide simultaneous details about the mass and size of a protein ion, which is useful for structural biology applications. In the following chapters of this dissertation, various MS, LC, and IM techniques were used to study protein composition, interactions, and structure. In Chapter 2, native anion exchange chromatography (AEX), which uses a low-pressure and bioinert workflow with ammonium acetate mobile phases, is used to enhance native MS by removing common contaminants that interfere with MS, separating protein variants based on their charge, and isolating proteins from complex mixtures. Those capabilities demonstrate that native AEX is a valuable tool for overcoming common challenges that are associated with native MS, and that native AEX has distinct advantages over other conventional sample preparation methods. In Chapter 3, an integrative MS strategy was developed to identify binding interactions that essential proteins called E3 ligases have in cells. Eukaryotic cells use hundreds of unique E3 ligases to control cellular functions through protein degradation. E3 ligases selectively bind to protein substrates through recognition motifs known as degrons. The integrative MS strategy, which uses a combination of native MS, native top-down MS, MS of destabilized samples, and LC-MS, is called "degronomics" and will be useful for uncovering degron motifs that other E3s can recognize in cells. Results from that study demonstrate that an E3 ligase called KLHDC2 binds to C-terminus diglycine degrons by uncovering KLHDC2-peptide binding interactions from cells. In Chapter 4, a combination of IM-MS experiments and computational tools was used to characterize the effects of charge state, charge distribution, and structure on the ion mobility of proteins in nitrogen gas. The results from Chapter 4 have implications for interpreting protein structures from IM-MS experiments and for comparing nitrogen gas-based measurements against measurements taken with helium gas.

Proteomics

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Publisher : Springer Science & Business Media
ISBN 13 : 0306468956
Total Pages : 128 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Proteomics by : Timothy Palzkill

Download or read book Proteomics written by Timothy Palzkill and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics is an introduction to the exciting new field of proteomics, an interdisciplinary science that includes biology, bioinformatics, and protein chemistry. The purpose of this book is to provide the active researcher with an overview of the types of questions being addressed in proteomics studies and the technologies used to address those questions. Key subjects covered in this book include: an assessment of the limitations of this approach and outlines new developments in mass spectrometry that will advance future research high-throughput recombinant DNA cloning methods used to systematically clone all of the open reading frames of an organism into plasmid vectors for large scale protein expression and functional studies such as protein-protein interactions with the two-hybrid system protein structure an overview of large-scale experimental attempts to determine the three-dimensional structures of representative sets of proteins computational approaches to determining the three-dimensional structure of proteins. Proteomics provides a starting point for researchers who would like a theoretical understanding of the new technologies in the field, and obtain a solid grasp of the fundamentals before integrating new tools into their experiments. Written with attention to detail, but without being overwhelmingly technical, Proteomics is a user-friendly guide needed by most biologists today.

Neuroproteomics

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Publisher : CRC Press
ISBN 13 : 1420076264
Total Pages : 356 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Neuroproteomics by : Oscar Alzate

Download or read book Neuroproteomics written by Oscar Alzate and published by CRC Press. This book was released on 2009-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg

Methods in Proteome and Protein Analysis

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Publisher : Springer Science & Business Media
ISBN 13 : 3662087227
Total Pages : 429 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Methods in Proteome and Protein Analysis by : Roza Maria Kamp

Download or read book Methods in Proteome and Protein Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, Methods in Proteome and Protein Analysis presents the most interesting papers from the 14th MPSA meeting. Major topics include: protein and peptide sample preparation and separation; new reagent for protein sequence analysis; mass spectrometry in protein research; analysis of posttranslational modification; protein-protein interaction using MALDI-MS; manipulation of genome or functional compositon trap; structure-function correlation study using optical biosensors of microcolorimetrical techniques; structural proteomics as NMR or fluorescence polarization study; the classification and prediction of structure or functional sites; in silico analysis of proteins and proteomes; increasing throughput and data quality for proteomics.