Mass Spectrometry Based Lipidomics Applied To Animal And Clinical Studies

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Mass Spectrometry-Based Lipidomics

Author : Fong-Fu Hsu
Publisher : Humana
ISBN 13 : 9781071614099
Total Pages : 314 pages
Book Rating : 4.6/5 (14 download)

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Book Synopsis Mass Spectrometry-Based Lipidomics by : Fong-Fu Hsu

Download or read book Mass Spectrometry-Based Lipidomics written by Fong-Fu Hsu and published by Humana. This book was released on 2021-05-29 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

Metabolic Profiling

Author : Georgios A. Theodoridis
Publisher : Humana
ISBN 13 : 9781493992522
Total Pages : 291 pages
Book Rating : 4.9/5 (925 download)

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Book Synopsis Metabolic Profiling by : Georgios A. Theodoridis

Download or read book Metabolic Profiling written by Georgios A. Theodoridis and published by Humana. This book was released on 2019-04-30 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.

Chemical Derivatization in Gas Chromatography

Author : J. Drozd
Publisher : Elsevier
ISBN 13 : 0080858201
Total Pages : 247 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Chemical Derivatization in Gas Chromatography by : J. Drozd

Download or read book Chemical Derivatization in Gas Chromatography written by J. Drozd and published by Elsevier. This book was released on 1986-07-01 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Derivatization in Gas Chromatography

Lipidomics

Author : Sanjoy K. Bhattacharya
Publisher : Humana
ISBN 13 : 9781071629680
Total Pages : 0 pages
Book Rating : 4.6/5 (296 download)

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Book Synopsis Lipidomics by : Sanjoy K. Bhattacharya

Download or read book Lipidomics written by Sanjoy K. Bhattacharya and published by Humana. This book was released on 2024-02-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.

Direct Analysis in Real Time Mass Spectrometry

Author : Yiyang Dong
Publisher : John Wiley & Sons
ISBN 13 : 3527341846
Total Pages : 370 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Direct Analysis in Real Time Mass Spectrometry by : Yiyang Dong

Download or read book Direct Analysis in Real Time Mass Spectrometry written by Yiyang Dong and published by John Wiley & Sons. This book was released on 2018-04-09 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clear, comprehensive, and state of the art, the groundbreaking book on the emerging technology of direct analysis in real time mass spectrometry Written by a noted expert in the field, Direct Analysis in Real Time Mass Spectrometry offers a review of the background and the most recent developments in DART-MS. Invented in 2005, DART-MS offers a wide range of applications for solving numerous analytical problems in various environments, including food science, forensics, and clinical analysis. The text presents an introduction to the history of the technology and includes information on the theoretical background, for exampleon the ionization mechanism. Chapters on sampling and coupling to different types of mass spectrometers are followed by a comprehensive discussion of a broad range of applications. Unlike most other ionization methods, DART does not require laborious sample preparation, as ionization takes place directly on the sample surface. This makes the technique especially attractive for applications in forensics and food science. Comprehensive in scope, this vital text: -Sets the standard on an important and emerging ionization technique -Thoroughly discusses all the relevant aspects from instrumentation to applications -Helps in solving numerous analytical problems in various applications, for example food science, forensics, environmental and clinical analysis -Covers mechanisms, coupling to mass spectrometers, and includes information on challenges and disadvantages of the technique Academics, analytical chemists, pharmaceutical chemists, clinical chemists, forensic scientists, and others will find this illuminating text a must-have resource for understanding the most recent developments in the field.

Lipidomics

Author : Xianlin Han
Publisher : John Wiley & Sons
ISBN 13 : 1118893123
Total Pages : 48 pages
Book Rating : 4.1/5 (188 download)

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Book Synopsis Lipidomics by : Xianlin Han

Download or read book Lipidomics written by Xianlin Han and published by John Wiley & Sons. This book was released on 2016-05-02 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the area of lipidomics from fundamentals and theory to applications Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes

Processing Metabolomics and Proteomics Data with Open Software

Author : Robert Winkler
Publisher : Royal Society of Chemistry
ISBN 13 : 1788017218
Total Pages : 460 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Processing Metabolomics and Proteomics Data with Open Software by : Robert Winkler

Download or read book Processing Metabolomics and Proteomics Data with Open Software written by Robert Winkler and published by Royal Society of Chemistry. This book was released on 2020-03-19 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics and proteomics allow deep insights into the chemistry and physiology of biological systems. This book expounds open-source programs, platforms and programming tools for analysing metabolomics and proteomics mass spectrometry data. In contrast to commercial software, open-source software is created by the academic community, which facilitates the direct interaction between users and developers and accelerates the implementation of new concepts and ideas. The first section of the book covers the basics of mass spectrometry, experimental strategies, data operations, the open-source philosophy, metabolomics, proteomics and statistics/ data mining. In the second section, active programmers and users describe available software packages. Included tutorials, datasets and code examples can be used for training and for building custom workflows. Finally, every reader is invited to participate in the open science movement.

Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines

Author : Aihua Zhang
Publisher : John Wiley & Sons
ISBN 13 : 3527835741
Total Pages : 292 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines by : Aihua Zhang

Download or read book Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines written by Aihua Zhang and published by John Wiley & Sons. This book was released on 2021-08-20 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highlights the importance and benefit of mass spectrometry-based metabolomics for identifying biomarkers that accurately screen for potential biomarkers of diseases Mass spectrometry-based metabolomics offer new opportunities for biomarker discovery in complex diseases and may provide pathological understanding of diseases beyond traditional technologies. It is the systematic analysis of low-molecular-weight metabolites in biological samples and has been applied to discovering and identifying the perturbed pathways. Currently, mass spectrometry-based metabolomics has become an important tool in clinical research and the diagnosis of human disease. Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines comprehensively presents the current state, challenges, and applications of high-throughput mass spectrometry-based metabolomics such as metabolites analysis, biomarker discovery, technical challenges, discovery of natural product, mechanism interpretation of action, discovery of active ingredients, clinical application and precision medicine, and enhancing their biomedical value in a real world of biomedicine, shedding light on the potential for spectrometry-based metabolomics. It highlights the value of mass spectrometry-based metabolomics and metabolism to address the complexity of herbal medicines in systems pharmacology, especially, to link phytochemical analysis with the assessment of pharmacological effect and therapeutic potential. Each chapter has been laid out with introduction, method, up-to-date literature, identification of biomarker, and applications Covers the current state, challenges, and applications of high-throughput mass spectrometry-based metabolomics in the discovery of biomarker, active ingredients, natural product, etc. Constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of new techniques Provides a guide for new practitioners of pharmacologists, pharmacological scholars, drug developers, botanist, researchers of traditional medicines. Mass Spectrometry-Based Metabolomics in Clinical and Herbal Medicines provides a landmark of mass spectrometry-based metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all biomedical science fields.

Methodologies for Metabolomics

Author : Norbert W. Lutz
Publisher : Cambridge University Press
ISBN 13 : 0521765900
Total Pages : 641 pages
Book Rating : 4.5/5 (217 download)

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Book Synopsis Methodologies for Metabolomics by : Norbert W. Lutz

Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Mass Spectrometry for the Clinical Laboratory

Author : Hari Nair
Publisher : Academic Press
ISBN 13 : 0128009926
Total Pages : 306 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Mass Spectrometry for the Clinical Laboratory by : Hari Nair

Download or read book Mass Spectrometry for the Clinical Laboratory written by Hari Nair and published by Academic Press. This book was released on 2016-11-02 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab. Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab

Differential Ion Mobility Spectrometry

Author : Alexandre A. Shvartsburg
Publisher : CRC Press
ISBN 13 : 9781420051070
Total Pages : 322 pages
Book Rating : 4.0/5 (51 download)

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Book Synopsis Differential Ion Mobility Spectrometry by : Alexandre A. Shvartsburg

Download or read book Differential Ion Mobility Spectrometry written by Alexandre A. Shvartsburg and published by CRC Press. This book was released on 2008-12-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.

Lipidomics

Author : Kim Ekroos
Publisher : John Wiley & Sons
ISBN 13 : 3527655964
Total Pages : 507 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Lipidomics by : Kim Ekroos

Download or read book Lipidomics written by Kim Ekroos and published by John Wiley & Sons. This book was released on 2012-09-04 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on the practical applications, this user-oriented guide presents current technologies and strategies for systems-level lipid analysis, going beyond basic research to concentrate on commercial uses of lipidomics in biomarker and diagnostic development, as well as within pharmaceutical drug discovery and development. The editor and authors have experience of the most recent analytical instruments and techniques, allowing them to provide here first-hand practical experience for newcomers to the field. The first half of the book covers current methodologies, ranging from global to targeted lipidomics and shotgun approaches, while the second part discusses the role of lipidomics in biomedical and pharmaceutical research, covering such diverse fields as inflammation, metabolic syndrome, cardiovascular and neurological disease. Both small and large-scale, high-throughput approaches are discussed, resulting in an invaluable source for academic and industrial research and development.

Tandem Mass Spectrometry of Lipids

Author : Robert C Murphy
Publisher : Royal Society of Chemistry
ISBN 13 : 1782626352
Total Pages : 295 pages
Book Rating : 4.7/5 (826 download)

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Book Synopsis Tandem Mass Spectrometry of Lipids by : Robert C Murphy

Download or read book Tandem Mass Spectrometry of Lipids written by Robert C Murphy and published by Royal Society of Chemistry. This book was released on 2014-12-02 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emerging field of lipidomics has been made possible because of advances in mass spectrometry, and in particular tandem mass spectrometry of lipid ions generated by electrospray ionization. The ability to carry out basic biochemical studies of lipids using electrospray ionization is predicated upon understanding the behaviour of lipid derived ions following collision induced decomposition and mechanisms of product ion formation. During the past 20 years, a wealth of information has been generated about lipid molecules that are now analysed by mass spectrometry, however there is no central source where one can obtain basic information about how these very diverse biomolecules behave following collisional activation. This book brings together, in one volume, this information so that investigators considering using tandem mass spectrometry to structurally characterize lipids or to quantitate their occurrence in a biological matrix, will have a convenient source to review mechanism of decomposition reactions related to the diversity of lipid structures. A separate chapter is devoted to each of seven major lipid classes including fatty acids, eicosanoids and bioactive lipid mediators, fatty acyl esters and amides, glycerol esters, glycerophospholipids, sphingolipids, and steroids. Mechanistic details are provided for understanding the pathways of formation of major product ions and ions used for structural characterization. In most cases specific ancillary information has been critical to understand the pathways, including isotope labeling and high resolution analysis of precursor and product ions. For a few specific examples such data is missing and pathways are proposed as a means to initiate further mass spectral experiments to prove or disprove pathway hypotheses. While this work largely centres on the lipid biochemistry of animal (mammalian) systems, general principles can be taken from the specific examples and applied to lipid biochemistry found in plants, fungi, prokaryotes and archeal organisms.

Plant Metabolomics

Author : Kazuki Saito
Publisher : Springer Science & Business Media
ISBN 13 : 3540297820
Total Pages : 351 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Plant Metabolomics by : Kazuki Saito

Download or read book Plant Metabolomics written by Kazuki Saito and published by Springer Science & Business Media. This book was released on 2006-06-29 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields.

LIPIDAT A Database of Thermo Data and Association Information on Lipid

Author : Martin Caffrey
Publisher : CRC Press
ISBN 13 : 9780849389245
Total Pages : 342 pages
Book Rating : 4.3/5 (892 download)

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Book Synopsis LIPIDAT A Database of Thermo Data and Association Information on Lipid by : Martin Caffrey

Download or read book LIPIDAT A Database of Thermo Data and Association Information on Lipid written by Martin Caffrey and published by CRC Press. This book was released on 1993-06-04 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: LIPIDAT is a convenient compilation of thermodynamic data and bibliographic information on lipids. Over 11,000 records in 15 information fields are provided. The book presents tabulations of all known mesomorphic and polymorphic phase transition types, temperatures, and enthalpies for synthetic and biologically derived lipids in dry, partially hydrated, and fully hydrated states. It also includes the effect of pH, protein, drugs, salt, and metal ion concentration on these thermodynamic values. Methods used in making the measurements and the experimental conditions are reported. Bibliographic information includes a complete literature reference and list of authors. The book will be an indispensable reference for biophysicists, chemical engineers, pharmaceutical and cosmetic researchers, dermatologists, nutritionists, biochemists, physiologists, food scientists, and fats and oils chemists.

The Handbook of Metabonomics and Metabolomics

Author : John C. Lindon
Publisher : Elsevier
ISBN 13 : 0080468004
Total Pages : 573 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis The Handbook of Metabonomics and Metabolomics by : John C. Lindon

Download or read book The Handbook of Metabonomics and Metabolomics written by John C. Lindon and published by Elsevier. This book was released on 2011-08-11 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular biology operates at three levels – genes, proteins and metabolites. This book is unique in that it provides a comprehensive description of an approach (metabonomics) to characterise the endogenous metabolites in a living system, complementing gene and protein studies (genomics and proteomics). These "omics" methods form the basis for understanding biology at a systems level. The Handbook of Metabonomics and Metabolomics aims to be the definitive work on the rapidly expanding subjects of metabolic profiling, metabolite and biomarker identification, encompassing the fields of metabonomics and metabolomics. It covers the principles of the subject, the analytical and statistical techniques used and the wide variety of applications.* comprehensive description of an approach (metabonomics) to characterise the endogenous metabolites in a living system, complementing gene and protein studies* aims to be the definitive work on the rapidly expanding subjects of metabolic profiling, metabolite and biomarker identification* covers the principles of the subject, the analytical and statistical techniques used and the wide variety of applications.

Lipid Analysis

Author : W. W. Christie
Publisher : Elsevier
ISBN 13 : 0857097865
Total Pages : 447 pages
Book Rating : 4.8/5 (57 download)

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Book Synopsis Lipid Analysis by : W. W. Christie

Download or read book Lipid Analysis written by W. W. Christie and published by Elsevier. This book was released on 2010-01-10 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.