Mammalian Cell Engineering

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Mammalian Cell Engineering

Author : Ryosuke Kojima
Publisher : Humana
ISBN 13 : 9781071614433
Total Pages : 330 pages
Book Rating : 4.6/5 (144 download)

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Book Synopsis Mammalian Cell Engineering by : Ryosuke Kojima

Download or read book Mammalian Cell Engineering written by Ryosuke Kojima and published by Humana. This book was released on 2022-07-21 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.

Cell Culture Engineering

Author : Gyun Min Lee
Publisher : John Wiley & Sons
ISBN 13 : 3527343342
Total Pages : 436 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Cell Culture Engineering by : Gyun Min Lee

Download or read book Cell Culture Engineering written by Gyun Min Lee and published by John Wiley & Sons. This book was released on 2020-01-13 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

Cell Line Development

Author : Mohamed Al-Rubeai
Publisher : Springer Science & Business Media
ISBN 13 : 9048122457
Total Pages : 259 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis Cell Line Development by : Mohamed Al-Rubeai

Download or read book Cell Line Development written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2009-08-11 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Cell and Tissue Reaction Engineering

Author : Regine Eibl
Publisher : Springer Science & Business Media
ISBN 13 : 3540681825
Total Pages : 367 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis Cell and Tissue Reaction Engineering by : Regine Eibl

Download or read book Cell and Tissue Reaction Engineering written by Regine Eibl and published by Springer Science & Business Media. This book was released on 2008-09-30 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.

Cell Culture Engineering

Author : Wei-Shu Hu
Publisher : Springer
ISBN 13 : 3540340076
Total Pages : 179 pages
Book Rating : 4.5/5 (43 download)

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Book Synopsis Cell Culture Engineering by : Wei-Shu Hu

Download or read book Cell Culture Engineering written by Wei-Shu Hu and published by Springer. This book was released on 2006-08-16 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.

Sophisticated Mammalian Cell Engineering with CRISPR/Cas12a

Author : Hannah Rose Kempton
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (133 download)

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Book Synopsis Sophisticated Mammalian Cell Engineering with CRISPR/Cas12a by : Hannah Rose Kempton

Download or read book Sophisticated Mammalian Cell Engineering with CRISPR/Cas12a written by Hannah Rose Kempton and published by . This book was released on 2022 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of CRISPR/Cas systems for programmable DNA manipulation has revolutionized the field of genome engineering. In addition to their use for cutting DNA, nuclease-dead versions of Cas proteins fused to different effectors are a powerful tool for cell engineering and synthetic biology. In this thesis, I will discuss two projects from my Ph.D., both demonstrating how Cas12a can be used for constructing next-generation genetic circuits in human cells. For my first project I developed a split Cas12a protein, which spontaneously re-assembles into a functional gene-activating effector when all components are present. Therapeutic cell engineering, in which cells are programmed to sense and respond to complex disease states, requires integration of information about the cellular environment and execution of a functional response only in response to the specified combination of inputs. By linking the expression of individual components of the split Cas12a system to different input signals, it forms the functional core of multi-input, multi-output logic circuits in mammalian cells. The system is highly programmable and can generate expandable AND gates, as well as incorporate NOT logic by using anti-CRISPR proteins as an OFF switch. For my second project I established a platform for parallel signal recording using a hyper-efficient Cas12a base editor. Biological signal recording enables the study of molecular inputs experienced throughout cellular history, by encoding a transient signal as a permanent, targeted DNA mutation that can be read out at a later time by sequencing. However, current methods are often limited in their ability to scale up beyond a single biologically relevant signal in mammalian contexts. This novel implementation of Cas12a for signal recording provides a powerful platform for scalable recording, demonstrated by the recording of up to four signals in parallel in human cells. This method is plug-and-play with diverse inputs in different cell types, including tumor antigen recording in CAR-T cells. It can also be easily extended for more sophisticated applications, such as time-delimited recording and dual recorder-effector cells. Overall, these two projects demonstrate the utility of Cas-based effectors in complex genetic circuits, for both understand-ing fundamental biological processes as well as constructing smarter cellular therapeutics.

Cell Engineering

Author : Mohamed Al-Rubeai
Publisher : Springer Science & Business Media
ISBN 13 : 0585379718
Total Pages : 311 pages
Book Rating : 4.5/5 (853 download)

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Book Synopsis Cell Engineering by : Mohamed Al-Rubeai

Download or read book Cell Engineering written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2007-11-23 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrating advances in molecular biology into bioprocesses presents a continuous challenge to scientists and bioengineers. This series is conceived to help meet this challenge. It examines and assesses the feasibility of new approaches for the modification of cellular function such as gene expression, protein processing, secretion, glycosylation, immortalisation, proliferation, and apoptosis as well as the systematic study of the metabolic genotype-phenotype relationship. The series provides detailed coverage of the methodology for improving cellular properties of cells used in the production of biopharmaceuticals, gene and cell therapies and tissue engineering. It also seeks to explain the cellular mechanisms underlying in vitro physiological activity and productivity. This volume, which is based on presentations at the `European Workshop on Animal Cell Engineering' held in Costa Brava, Spain, contains a collection of chapters relating to cellular function and modification by leading authorities in several different areas of basic research and the biopharmaceutical industry.

Cell Culture Engineering

Author : Gyun Min Lee
Publisher : John Wiley & Sons
ISBN 13 : 3527343342
Total Pages : 436 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Cell Culture Engineering by : Gyun Min Lee

Cell Engineering

Author : Mohamed Al-Rubeai
Publisher : Springer Science & Business Media
ISBN 13 : 9401143153
Total Pages : 366 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Cell Engineering by : Mohamed Al-Rubeai

Download or read book Cell Engineering written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infection cycle, two forms of the virus are produced: an early budded virus (BY) form (Kost et al. , 2000), in which the viral DNA and structural proteins are surrounded by membrane derived from the infected cell; and a late occluded form (occlusion-derived virus, ODy), consisting of enveloped viral cores which are embedded in a crystal matrix of viral proteins. The principal component of the matrix is the abundantly expressed protein polyhedrin. The budded virus rapidly spreads the infection from cell to cell within the insect host, resulting ultimately in the complete liquefaction of the host, and release of occluded virus into the environment. The occluded form protects the released virus, allowing it to survive for long periods in the environment until ingested by another host. In the alkaline environment ofthe insect gut, the protective protein matrix is removed, and the life cycle is repeated. In insect cell cultures, only the BV form of baculovirus is required, and the polyhedrin gene may be replaced with the gene for the recombinant protein. An additional benefit of replacing or deleting polyhedrin is that it effectively makes the virus unable to survive outside the laboratory, an advantage in terms of environmental safety. The system is intrinsically safe to animals, being unable to replicate in species other than a limited range of insects.

Cell Culture Engineering and Technology

Author : Ralf Pörtner
Publisher : Springer Nature
ISBN 13 : 3030798712
Total Pages : 552 pages
Book Rating : 4.0/5 (37 download)

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Book Synopsis Cell Culture Engineering and Technology by : Ralf Pörtner

Download or read book Cell Culture Engineering and Technology written by Ralf Pörtner and published by Springer Nature. This book was released on 2022-02-20 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume is dedicated towards the progress achieved within the last years in all areas of Cell Culture Engineering and Technology. It comprises contributions of active researchers in the field of cell culture development for the production of recombinant proteins, cell line development, cell therapy and gene therapy, with consideration of media development, process scale-up, reactor design, monitoring and control and model-assisted strategies for process design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. This book is conceived for graduate students, postdoctoral fellows and researchers interested in the latest developments in Cell Engineering.

The Science and Applications of Synthetic and Systems Biology

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309219396
Total Pages : 570 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis The Science and Applications of Synthetic and Systems Biology by : Institute of Medicine

Download or read book The Science and Applications of Synthetic and Systems Biology written by Institute of Medicine and published by National Academies Press. This book was released on 2011-12-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.

Mammalian Cell Cultures for Biologics Manufacturing

Author : Weichang Zhou
Publisher : Springer
ISBN 13 : 3642540503
Total Pages : 262 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Mammalian Cell Cultures for Biologics Manufacturing by : Weichang Zhou

Download or read book Mammalian Cell Cultures for Biologics Manufacturing written by Weichang Zhou and published by Springer. This book was released on 2014-01-15 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Cell Culture Bioprocess Engineering, Second Edition

Author : Wei-Shou Hu
Publisher : CRC Press
ISBN 13 : 1498762867
Total Pages : 466 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis Cell Culture Bioprocess Engineering, Second Edition by : Wei-Shou Hu

Download or read book Cell Culture Bioprocess Engineering, Second Edition written by Wei-Shou Hu and published by CRC Press. This book was released on 2020-03-06 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

Genomics and Systems Biology of Mammalian Cell Culture

Author : Wei-Shou Hu
Publisher : Springer Science & Business Media
ISBN 13 : 3642283497
Total Pages : 305 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis Genomics and Systems Biology of Mammalian Cell Culture by : Wei-Shou Hu

Download or read book Genomics and Systems Biology of Mammalian Cell Culture written by Wei-Shou Hu and published by Springer Science & Business Media. This book was released on 2012-03-16 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcriptome Analysis, by Frank Stahl, Bernd Hitzmann, Kai Mutz, Daniel Landgrebe, Miriam Lübbecke, Cornelia Kasper, Johanna Walter und Thomas Scheper Transcriptome Data Analysis for Cell Culture Processes, by Marlene Castro-Melchor, Huong Le und Wei-Shou Hu Modeling Metabolic Networks for Mammalian Cell Systems: General Considerations, Modeling Strategies, and Available Tools, by Ziomara P. Gerdtzen Metabolic Flux Analysis in Systems Biology of Mammalian Cells, by Jens Niklas und Elmar Heinzle Advancing Biopharmaceutical Process Development by System-Level Data Analysis and Integration of Omics Data, by Jochen Schaub, Christoph Clemens, Hitto Kaufmann und Torsten W. Schulz Protein Glycosylation and Its Impact on Biotechnology, by Markus Berger, Matthias Kaup und Véronique Blanchard Protein Glycosylation Control in Mammalian Cell Culture: Past Precedents and Contemporary Prospects, by Patrick Hossler Modeling of Intracellular Transport and Compartmentation, by Uwe Jandt und An-Ping Zeng Genetic Aspects of Cell Line Development from a Synthetic Biology Perspective, by L. Botezatu, S. Sievers, L. Gama-Norton, R. Schucht, H. Hauser und D. Wirth.

Cell Engineering and Modulation of Metabolic Pathways in Mammalian Cells for the Production of Glycoprotein and Proteoglycan Based Pharmaceuticals

Download Cell Engineering and Modulation of Metabolic Pathways in Mammalian Cells for the Production of Glycoprotein and Proteoglycan Based Pharmaceuticals PDF Online Free

Author : Payel Datta
Publisher :
ISBN 13 :
Total Pages : 242 pages
Book Rating : 4.:/5 (115 download)

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Book Synopsis Cell Engineering and Modulation of Metabolic Pathways in Mammalian Cells for the Production of Glycoprotein and Proteoglycan Based Pharmaceuticals by : Payel Datta

Download or read book Cell Engineering and Modulation of Metabolic Pathways in Mammalian Cells for the Production of Glycoprotein and Proteoglycan Based Pharmaceuticals written by Payel Datta and published by . This book was released on 2013 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cell Engineering

Author : Mohamed Al-Rubeai
Publisher : Springer Science & Business Media
ISBN 13 : 9781402022166
Total Pages : 354 pages
Book Rating : 4.0/5 (221 download)

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Book Synopsis Cell Engineering by : Mohamed Al-Rubeai

Download or read book Cell Engineering written by Mohamed Al-Rubeai and published by Springer Science & Business Media. This book was released on 2004-07-13 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ten papers from the European Workshop on Animal Cell Engineering Costa Brava, Spain (no date noted) kick off a series designed to facilitate the integration of developments in molecular biology into bioprocesses . Scientists and engineers doing basic research and from the biopharmaceutical industry discuss gene expression, protein synthesis and modification, cell proliferation, immortalization, and apoptosis. Their titles include understanding the translation regulatory mechanisms to improve the efficiency and the specificity of protein production by the cell factory, using the endoprotease furin in the high-yield expression of recombinant proteins requiring proteolytic maturation, inhibiting apoptosis in mammalian cell culture, factors involved in the cell cycle arrest of adult rat cardiomycytes, and the immortalization of hepatocytes through the targeted deregulation of the cell cycle. Annotation copyrighted by Book News, Inc., Portland, OR

Animal Cell Culture

Author : Mohamed Al-Rubeai
Publisher : Springer
ISBN 13 : 3319103202
Total Pages : 766 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Animal Cell Culture by : Mohamed Al-Rubeai

Download or read book Animal Cell Culture written by Mohamed Al-Rubeai and published by Springer. This book was released on 2014-11-28 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.