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Author : Mary Jane Fukushima Yagi
Publisher :
ISBN 13 :
Total Pages : 236 pages
Book Rating : 4.:/5 (29 download)
Download or read book Long Term Cell Culture of Mannary Tumor Cells written by Mary Jane Fukushima Yagi and published by . This book was released on 1971 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Roswitha Pfragner
Publisher : John Wiley & Sons
ISBN 13 : 0471270628
Total Pages : 453 pages
Book Rating : 4.4/5 (712 download)
Download or read book Culture of Human Tumor Cells written by Roswitha Pfragner and published by John Wiley & Sons. This book was released on 2005-03-11 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ein neuer Band aus der 'Culture of Specialized Cells'-Reihe. Leserfreundlich aufgemacht. Er vermittelt spezifische praktische Details, wie man Medien und Reagenzien sowie Protokolle für Zellisolierung und Zellkultur präpariert. Logisch aufgebaut und nach spezifischen Tumoren gegliedert. Farbtafeln demonstrieren anschaulich Immunozytochemie und Fluoreszenz in situ Hybridisierung (FISH). Darüber hinaus beschreibt das Buch auch umfangreiche Sicherheitsvorkehrungen. Mit einer Vielzahl nützlicher Tipps. Mit einem Glossar zu ausgewählten Fachtermini. Enthält eine umfangreiche Liste mit Bezugsadressen von Ausrüstung und Zellkulturprodukten. Erläutert medikamentöse Behandlung, Auswahl, Differenzierung, Assays für die Untersuchung maligner Zellen sowie Risiken und Anwendungsmöglichkeiten.
Author :
Publisher :
ISBN 13 :
Total Pages : 32 pages
Book Rating : 4.:/5 (227 download)
Download or read book A New Paradigm for African American Breast Cancer Involving Stem Cell Differentiation in a Novel Cell Culture System written by and published by . This book was released on 2006 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our laboratory has published a novel culture system for Human Mammary Epithelial Cells (HMEC), both normal and malignant. This system allows for unusually long-term establishment of normal primary cultures that begin as three-dimensional "mammospheres," which subsequently differentiate into complex organotypic branching ducts and lobules that demonstrate Epithelial Specific Antibody (ESA) staining, lumen, polarized nuclei, desmosomes along the lateral surfaces of the cells, and microvilli on the apical surfaces. We hypothesize that since we have demonstrated de novo formation of multicellular organotypic epithelial ductal and lobular structures, that our cultures must contain multipotent stem cells. We have established primary HMEC cultures from 35/35 breast reduction mammoplasty tissues. These tissues included: 19 pre-menopausal, 9 post-menopausal and 7 peri-menopausal (ages 45-55) subjects. Six out of 35 of the subjects from whom this tissue came, were African American and matched in socioeconomic status with the white women (middle class). We found: 1. The more children a woman had, the less likely her breast tissue was to form ductal structures in vitro consistent with the idea that lactational differentiation decreases the number of pluripotent stem cells in the breast. 2. Pre-menopausal breast tissue was more likely to form ductal structures in culture than post-menopausal tissue. 3. Race was shown to contribute as a modifying factor in the ability to form ductal architecture in culture, with AA tissue demonstrating more of an ability to spontaneously differentiate than white tissue.
Author : Daniel Medina
Publisher : Springer
ISBN 13 :
Total Pages : 540 pages
Book Rating : 4.3/5 (91 download)
Download or read book Cellular and Molecular Biology of Mammary Cancer written by Daniel Medina and published by Springer. This book was released on 1987 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea for this book arose during the 1985 Gordon Conference on "Mammary Gland Biology". New developments in the methodology of cell biology and the explosive growth of molecular biology had begun to impact upon our understanding of mammary gland growth and function. It seemed a propitious time for summarizing the current status of knowledge of the cell and molecular biology of mammary cancer and for attempting to outline future areas of concern and interest. The reviews presented here were completed by the Fall of 1986. Although new insights will surely continue to emerge, it is hoped that the material in this volume will form not only a current update but a basic core of information for future experiments. We have not attempted to cover all areas of mammary gland transformation. Those areas where recent detailed reviews are already available have been omitted. Also, the areas of normal gland development, cell ultrastructure, hormone responsiveness, chemotherapy and clinical aspects of mammary cancer have not been included. Instead, we have selected those areas where the development of new methodology, reagents and results have led to new ideas about mammary gland function and development as they are related to neoplasia.
Author : Charles M. McGrath
Publisher :
ISBN 13 :
Total Pages : 544 pages
Book Rating : 4.:/5 (49 download)
Download or read book Cell Biology of Breast Cancer written by Charles M. McGrath and published by . This book was released on 1980 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Benjamin S. Leung
Publisher : Springer Science & Business Media
ISBN 13 : 9401180458
Total Pages : 296 pages
Book Rating : 4.4/5 (11 download)
Download or read book Hormonal Regulation of Mammary Tumors written by Benjamin S. Leung and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept that hormones influence tumor growth originated in 1889 with the proposal of Albert Schinzinger who suggested that breast cancer is related to the ovaries. Several years later, Sir George Beatson observed that remission of disseminated breast cancer could be achieved in premenopausal patients by performing bilateral oophorectomy. As a result of the contri butions of Hedley Atkins, Charles Huggins and others, additive and ablative hormonal therapies have been widely used for the treatment of advanced breast cancers for several decades. Model systems to study the effects of hormones on growth and regression of breast tumors have been available for many years; however, the complexities of the hormonal environment have rendered in vivo studies difficult in man and experimental animals. Recently, the availability of long-term cultures of breast cancer cells has stimulated many investigators to use these cell lines to unravel the mechanisms of hormone action. Because of the extreme diversity and complexity of advances regarding the endocrinology of the breast and breast cancers, a multi-authored review was deemed necessary. It has been gratifying to receive contributions from many noted scholars. In Volume I of this monograph, the influence of steroid hormones and their antagonists upon normal and neoplastic tissues of the mammary gland are presented. In Volume II, the effects of peptide and other hormones are reviewed.
Author : Alecsandru Ioan Baba
Publisher :
ISBN 13 : 9789732714577
Total Pages : 787 pages
Book Rating : 4.7/5 (145 download)
Download or read book Comparative Oncology written by Alecsandru Ioan Baba and published by . This book was released on 2007 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Gordon Sato
Publisher :
ISBN 13 :
Total Pages : 570 pages
Book Rating : 4.F/5 ( download)
Download or read book Hormones and Cell Culture written by Gordon Sato and published by . This book was released on 1979 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Shay Soker
Publisher : Humana Press
ISBN 13 : 3319605119
Total Pages : 225 pages
Book Rating : 4.3/5 (196 download)
Download or read book Tumor Organoids written by Shay Soker and published by Humana Press. This book was released on 2017-10-20 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer cell biology research in general, and anti-cancer drug development specifically, still relies on standard cell culture techniques that place the cells in an unnatural environment. As a consequence, growing tumor cells in plastic dishes places a selective pressure that substantially alters their original molecular and phenotypic properties.The emerging field of regenerative medicine has developed bioengineered tissue platforms that can better mimic the structure and cellular heterogeneity of in vivo tissue, and are suitable for tumor bioengineering research. Microengineering technologies have resulted in advanced methods for creating and culturing 3-D human tissue. By encapsulating the respective cell type or combining several cell types to form tissues, these model organs can be viable for longer periods of time and are cultured to develop functional properties similar to native tissues. This approach recapitulates the dynamic role of cell–cell, cell–ECM, and mechanical interactions inside the tumor. Further incorporation of cells representative of the tumor stroma, such as endothelial cells (EC) and tumor fibroblasts, can mimic the in vivo tumor microenvironment. Collectively, bioengineered tumors create an important resource for the in vitro study of tumor growth in 3D including tumor biomechanics and the effects of anti-cancer drugs on 3D tumor tissue. These technologies have the potential to overcome current limitations to genetic and histological tumor classification and development of personalized therapies.
Author : Ian A. Cree
Publisher : Humana Press
ISBN 13 : 9781493956579
Total Pages : 502 pages
Book Rating : 4.9/5 (565 download)
Download or read book Cancer Cell Culture written by Ian A. Cree and published by Humana Press. This book was released on 2016-08-23 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.
Author : Jorgen Fogh
Publisher : Springer Science & Business Media
ISBN 13 : 1475716478
Total Pages : 568 pages
Book Rating : 4.4/5 (757 download)
Download or read book Human Tumor Cells in Vitro written by Jorgen Fogh and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of cultured human tumor cells is a most obvious approach in experimental human cancer research. For many techniques in virology, immunology, biochemistry, and biophysics, for example, large amounts of cells may be required and such quantities are usually provided only when the cultures develop into established cell lines; when this happens, thorough characterization also becomes possible. The development of cell lines, therefore, is of prime importance. Recent major advances in research with animal cell systems see m to be a prologue for present and future efforts directed toward work with human tumor cells in culture. Conceivably, the most significant results in cancer research may develop from work with such cells, and so the time seemed right to define the present state of our knowledge. This is the first book dedicated exclusively to the subject: human tumor cells in vitro. Although so me of the fundamental aspects in the cultivation of human tumor ceIls, and the extent to which they represent human cancer in vivo are still unclear, I asked a number of the leading investigators in this area of research to collect and evaluate previous and present contributions, and to offer their thoughts on the questions to which answers are not yet available. Many of the chapters are concerned with techniques of cultivation. Cultures from some types of tumors have grown weIl; in many cases they have given rise to established cell lines.
Author : Robert C. Bast, Jr.
Publisher : John Wiley & Sons
ISBN 13 : 111900084X
Total Pages : 2004 pages
Book Rating : 4.1/5 (19 download)
Download or read book Holland-Frei Cancer Medicine written by Robert C. Bast, Jr. and published by John Wiley & Sons. This book was released on 2017-03-10 with total page 2004 pages. Available in PDF, EPUB and Kindle. Book excerpt: Holland-Frei Cancer Medicine, Ninth Edition, offers a balanced view of the most current knowledge of cancer science and clinical oncology practice. This all-new edition is the consummate reference source for medical oncologists, radiation oncologists, internists, surgical oncologists, and others who treat cancer patients. A translational perspective throughout, integrating cancer biology with cancer management providing an in depth understanding of the disease An emphasis on multidisciplinary, research-driven patient care to improve outcomes and optimal use of all appropriate therapies Cutting-edge coverage of personalized cancer care, including molecular diagnostics and therapeutics Concise, readable, clinically relevant text with algorithms, guidelines and insight into the use of both conventional and novel drugs Includes free access to the Wiley Digital Edition providing search across the book, the full reference list with web links, illustrations and photographs, and post-publication updates
Author : Dania Movia
Publisher : Springer Nature
ISBN 13 : 1071630563
Total Pages : 291 pages
Book Rating : 4.0/5 (716 download)
Download or read book Cancer Cell Culture written by Dania Movia and published by Springer Nature. This book was released on 2023-05-18 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two contains technical chapters that illustrate step-by-step methodologies for specific cancer cell culture methods. The methods discussed range from the generation of isogenic cancer cell lines, the use of serum-free growth conditions, and three-dimensional cell cultures and their specific assays for the efficacy assessment of new anticancer therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cancer Cell Culture: Methods and Protocols is a valuable tool to help researchers involved in this important field to further improve or advance their models for cancer research.
Author : John R. W. Masters
Publisher : Springer Science & Business Media
ISBN 13 : 0792351436
Total Pages : 295 pages
Book Rating : 4.7/5 (923 download)
Download or read book Cancer Cell Lines Part 1 written by John R. W. Masters and published by Springer Science & Business Media. This book was released on 1999 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series provides the first complete guide to the culture of human cells. It is a detailed manual and reference resource for researchers in every aspect of medicine, biology, technology and immunobiology using human cells. Every chapter is written by an internationally recognised authority in the field. The first three volumes in the series provide a comprehensive text on the culture of established cell lines from every type of human cancer. They provide a basic manual and reference resource for every cancer research scientist using human cancer cells.
Author : Katherine Elaine McKenzie
Publisher :
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.:/5 (33 download)
Download or read book Lipid Involvement in the Proliferation of Mouse Mammary Tumor Cells in Collagen Gel Culture written by Katherine Elaine McKenzie and published by . This book was released on 1990 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Robert B. Dickson
Publisher : Springer Science & Business Media
ISBN 13 : 1461312590
Total Pages : 432 pages
Book Rating : 4.4/5 (613 download)
Download or read book Mammary Tumor Cell Cycle, Differentiation, and Metastasis written by Robert B. Dickson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mammary Tumor Cell Cycle, Differentiation and Metastasis is the fifth volume since 1988 in a series designed to broadly examine current advances in the cellular and molecular biology of breast cancer. As in previous volumes, the editors have invited recognized experts in cutting-edge topics to provide a chapter focused on their area of research. The editors have turned to the researchers who study rodent models of the disease and to those who study the cellular and molecular basis of human breast cancer. The first section of the book is devoted to new mouse models of mammary development and tumorigenesis. The second section moves to studies of human breast cancer and focuses on receptors, signalling, and the cell cycle. The final section deals with defective tissue interactions in human breast cancer. We are now in a period of extremely rapid accumulation of data on the molecular and cellular biology of breast cancer. These findings are highlighted in chapters from Mammary Tumor Cell Cycle, Differentiation and Metastasis: Advances in Cellular and Molecular Biology of Breast Cancer.
Author : Catherine Sue Murphy
Publisher :
ISBN 13 :
Total Pages : 346 pages
Book Rating : 4.:/5 (89 download)
Download or read book Control of Breast Cancer Cell Replication in Vitro by Estrogens and Antiestrogens written by Catherine Sue Murphy and published by . This book was released on 1990 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:
