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ISBN 13 :
Total Pages : 474 pages
Book Rating : 4.:/5 (82 download)
Download or read book Laboratory Methods in Cell Biology written by and published by . This book was released on 2012 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:
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Publisher : Academic Press
ISBN 13 : 0124078079
Total Pages : 447 pages
Book Rating : 4.1/5 (24 download)
Download or read book Laboratory Methods in Cell Biology: Imaging written by and published by Academic Press. This book was released on 2013-01-03 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone. - Covers the most important laboratory methods in cell biology - Chapters written by experts in their fields
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Publisher : Academic Press
ISBN 13 : 0124055494
Total Pages : 505 pages
Book Rating : 4.1/5 (24 download)
Download or read book Laboratory Methods in Cell Biology written by and published by Academic Press. This book was released on 2012-12-31 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. - Covers the most important laboratory methods in cell biology - Chapters written by experts in their fields
Author : Douglas J. Taatjes
Publisher : Springer Science & Business Media
ISBN 13 : 1592599931
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)
Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Author : Robert D. Goldman
Publisher : CSHL Press
ISBN 13 : 9780879696832
Total Pages : 658 pages
Book Rating : 4.6/5 (968 download)
Download or read book Live Cell Imaging written by Robert D. Goldman and published by CSHL Press. This book was released on 2005 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Author : Ammasi Periasamy
Publisher : Springer
ISBN 13 : 1461475139
Total Pages : 473 pages
Book Rating : 4.4/5 (614 download)
Download or read book Methods in Cellular Imaging written by Ammasi Periasamy and published by Springer. This book was released on 2013-05-27 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.
Author : Dmitri Papkovsky
Publisher : Humana Press
ISBN 13 : 9781493961405
Total Pages : 367 pages
Book Rating : 4.9/5 (614 download)
Download or read book Live Cell Imaging written by Dmitri Papkovsky and published by Humana Press. This book was released on 2016-08-23 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.
Author : David L. Spector
Publisher : CSHL Press
ISBN 13 : 0879697512
Total Pages : 384 pages
Book Rating : 4.8/5 (796 download)
Download or read book Basic Methods in Microscopy written by David L. Spector and published by CSHL Press. This book was released on 2006 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.
Author : Charles A. Ettensohn
Publisher : Gulf Professional Publishing
ISBN 13 : 9780124802797
Total Pages : 948 pages
Book Rating : 4.8/5 (27 download)
Download or read book Development of Sea Urchins, Ascidians, and Other Invertebrate Deuterostomes: Experimental Approaches written by Charles A. Ettensohn and published by Gulf Professional Publishing. This book was released on 2004-10-22 with total page 948 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical guide to experimental methods for studying the development of invertebrate deuterostomes, such as sea urchins, ascidians, hemichordates, and amphioxus. These model organisms are of contemporary and historical importance to the study of developmental biology, particularly genomic research. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. * Covers a wide range of methods, from classical embryology through modern genomics * Discusses animals related to vertebrates, providing a valuable evolutionary perspective * Includes a practical guide to the use of sea urchins in the teaching laboratory
Author : Sung-Bae Kim
Publisher : Humana
ISBN 13 : 9781071612606
Total Pages : 447 pages
Book Rating : 4.6/5 (126 download)
Download or read book Live Cell Imaging written by Sung-Bae Kim and published by Humana. This book was released on 2022-06-12 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.
Author : Rafael Yuste
Publisher :
ISBN 13 :
Total Pages : 848 pages
Book Rating : 4.3/5 (91 download)
Download or read book Imaging Neurons written by Rafael Yuste and published by . This book was released on 2000 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.
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Publisher : Academic Press
ISBN 13 : 0124202012
Total Pages : 609 pages
Book Rating : 4.1/5 (242 download)
Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Author : Kursad Turksen
Publisher : Humana
ISBN 13 : 9781071606261
Total Pages : 0 pages
Book Rating : 4.6/5 (62 download)
Download or read book Imaging and Tracking Stem Cells written by Kursad Turksen and published by Humana. This book was released on 2020-06-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated book brings together protocols to arm stem cell biologists with tools and approaches to continue uncovering the intricacies and regulatory mechanisms underlying stem cell biology. Through various models and organ systems, the volume reflects the numerous recent advances in cell lineage and lineage tracking. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Imaging and Tracking Stem Cells: Methods and Protocols, Second Edition is an ideal guide for novices and experts alike who are working to expand our knowledge in the field of stem cells.
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Publisher : Academic Press
ISBN 13 : 0128208082
Total Pages : 358 pages
Book Rating : 4.1/5 (282 download)
Download or read book Expansion Microscopy for Cell Biology written by and published by Academic Press. This book was released on 2021-01-19 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more. Provides the authority and expertise of leading contributors from an international board of authors Represents the latest release in the Methods in Cell Biology series Includes the latest information on Expansion Microscopy for Cell Biology
Author : Francesco S. Pavone
Publisher : John Wiley & Sons
ISBN 13 : 3527632255
Total Pages : 229 pages
Book Rating : 4.5/5 (276 download)
Download or read book Laser Imaging and Manipulation in Cell Biology written by Francesco S. Pavone and published by John Wiley & Sons. This book was released on 2011-07-28 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, the editor has gathered a team of international experts to present the latest advances in the field of laser imaging and manipulation techniques. The result is broad coverage of the interactions with biological samples to perform novel optical manipulation operations, both on the cellular and tissue levels. Of interest to physicists working and researching laser tissue mechanisms, cell biologists investigating new imaging and manipulation operation on the cellular level, medical doctors working with new laser therapies and diagnostic tools, as well as engineers developing new technologies in the field of optics and lasers.
Author : Heather B. Miller
Publisher : Academic Press
ISBN 13 : 0123855454
Total Pages : 227 pages
Book Rating : 4.1/5 (238 download)
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
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Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
