Label Free Monitoring Of Cells In Vitro

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Label-Free Monitoring of Cells in vitro

Author : Joachim Wegener
Publisher : Springer Nature
ISBN 13 : 3030324338
Total Pages : 286 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Label-Free Monitoring of Cells in vitro by : Joachim Wegener

Download or read book Label-Free Monitoring of Cells in vitro written by Joachim Wegener and published by Springer Nature. This book was released on 2019-11-18 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.

Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors

Author : Maximilian Oberleitner
Publisher : Springer
ISBN 13 : 331945384X
Total Pages : 391 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors by : Maximilian Oberleitner

Download or read book Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors written by Maximilian Oberleitner and published by Springer. This book was released on 2017-09-26 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.

Mammalian Cell Viability

Author : Martin J. Stoddart
Publisher : Humana Press
ISBN 13 : 9781617791079
Total Pages : 0 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis Mammalian Cell Viability by : Martin J. Stoddart

Download or read book Mammalian Cell Viability written by Martin J. Stoddart and published by Humana Press. This book was released on 2011-04-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Toward A High Throughput Label-Free Platform for Monitoring Interaction Between Cells and Superparamagnetic Iron Oxide Nanoparticles

Download Toward A High Throughput Label-Free Platform for Monitoring Interaction Between Cells and Superparamagnetic Iron Oxide Nanoparticles PDF Online Free

Author : Sonia Abad Tan
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (136 download)

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Book Synopsis Toward A High Throughput Label-Free Platform for Monitoring Interaction Between Cells and Superparamagnetic Iron Oxide Nanoparticles by : Sonia Abad Tan

Download or read book Toward A High Throughput Label-Free Platform for Monitoring Interaction Between Cells and Superparamagnetic Iron Oxide Nanoparticles written by Sonia Abad Tan and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis focuses on cytotoxicity examination of superparamagnetic iron oxide nanoparticles (SPIONs) using different methods, including impedance spectroscopy. Despite the significant advances in adapting various biological and chemical methods to assess in-vitro toxicity of SPIONs, less attention has been paid on the development of a high throughput label-free screening platform to study the interaction between the cells and nanoparticles. In this thesis, we have taken the first step by proposing a label-free impedimetric method for monitoring cells treated with SPIONs. This study has demonstrated the effects of SPIONs on the adhesion, growth, proliferation, and viability of neuroblastoma 2A cells using impedance spectroscopy in comparison to other standard microscopic and cell viability testing methods. Results suggest that the change in impedance of electrodes exposed to the mixture of cells and SPIONs offers a wide dynamic range suitable for monitoring the effects of SPIONs with a concentration of less than 100 g/mL.

Basic Concepts on 3D Cell Culture

Author : Cornelia Kasper
Publisher : Springer Nature
ISBN 13 : 3030667499
Total Pages : 252 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis Basic Concepts on 3D Cell Culture by : Cornelia Kasper

Download or read book Basic Concepts on 3D Cell Culture written by Cornelia Kasper and published by Springer Nature. This book was released on 2021-06-09 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook shall introduce the students to 3D cell culture approaches and applications. An overview on existing techniques and equipment is provided and insight into various aspects and challenges that researchers need to consider and face during culture of 3D cells is given. The reader will learn the importance of physiological cell, tissue and organ models and gains important knowledge on 3D analytics. This textbook deepens selected aspects of the textbook “Cell Culture Technology”, which also is published in this series, while offering extended insight into 3D cell culture. The concept of the textbook encompasses various lectures ranging from basics in cell cultivation, tissue engineering, biomaterials and biocompatibility, in vitro test systems and regenerative medicine. The textbook addresses Master- and PhD students interested and/or working in the field of modern cell culture applications and will support the understanding of the essential strategies in 3D cell culture and waken awareness for the potentials and challenges of this application.

Chemical Genomics

Author : Haian Fu
Publisher : Cambridge University Press
ISBN 13 : 0521889480
Total Pages : 359 pages
Book Rating : 4.5/5 (218 download)

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Book Synopsis Chemical Genomics by : Haian Fu

Download or read book Chemical Genomics written by Haian Fu and published by Cambridge University Press. This book was released on 2012-02-13 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text covers new techniques and applications in chemical genomics for researchers, professionals and graduates in biology, biomedicine and chemistry.

Short-Term Versus Long-Term Challenges in Functional Biomaterials Interfacing Living Systems: Two Sides of the Coin

Author : Davide Ricci
Publisher : Frontiers Media SA
ISBN 13 : 2889712451
Total Pages : 302 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Short-Term Versus Long-Term Challenges in Functional Biomaterials Interfacing Living Systems: Two Sides of the Coin by : Davide Ricci

Download or read book Short-Term Versus Long-Term Challenges in Functional Biomaterials Interfacing Living Systems: Two Sides of the Coin written by Davide Ricci and published by Frontiers Media SA. This book was released on 2022-03-11 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tumor Immunology and Immunotherapy - Cellular Methods

Author : Nils-Petter Rudqvist
Publisher : Academic Press
ISBN 13 : 0128186739
Total Pages : 554 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Tumor Immunology and Immunotherapy - Cellular Methods by : Nils-Petter Rudqvist

Download or read book Tumor Immunology and Immunotherapy - Cellular Methods written by Nils-Petter Rudqvist and published by Academic Press. This book was released on 2020-01-15 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tumor Immunology and Immunotherapy - Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more. Contains the authority of authors who are leaders in their field Provides a comprehensive source on new methods and research in enzymology

Micro/Nano Cell and Molecular Sensors

Author : Ping Wang
Publisher : Springer
ISBN 13 : 9811016585
Total Pages : 242 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Micro/Nano Cell and Molecular Sensors by : Ping Wang

Download or read book Micro/Nano Cell and Molecular Sensors written by Ping Wang and published by Springer. This book was released on 2016-12-01 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on cell- and molecule-based biosensors using micro/nano devices as transducers. After providing basic information on micro/nano cell- and molecule-based biosensors, it introduces readers to the basic structures and properties of micro/nano materials and their applications. The topics covered provide a comprehensive review of the current state of the art in micro/nano cell- and molecule-based biosensors as well as their future development trends, ensuring the book will be of great interest to the interdisciplinary community active in this area: researchers, engineers, biologists, medical scientists, and all those whose work involves related interdisciplinary research and applications. Dr. Ping Wang is a Professor in Department of Biomedical Engineering at Zhejiang University, Hangzhou, China. Dr. Chunsheng Wu is a Professor in Medical School at Xi’an Jiaotong University, Xi’an, China. Dr. Ning Hu is an Assistant researcher in Department of Biomedical Engineering at Zhejiang University and a Postdoctoral researcher in Medical School at Harvard University, Boston, USA. Dr. K. Jimmy Hsia is a Professor in Department of Biomedical Engineering at Carnegie Mellon University, Pittsburgh, USA.

Monitoring and Evaluation of Biomaterials and their Performance In Vivo

Author : Roger Narayan
Publisher : Woodhead Publishing
ISBN 13 : 0081006047
Total Pages : 226 pages
Book Rating : 4.0/5 (81 download)

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Book Synopsis Monitoring and Evaluation of Biomaterials and their Performance In Vivo by : Roger Narayan

Download or read book Monitoring and Evaluation of Biomaterials and their Performance In Vivo written by Roger Narayan and published by Woodhead Publishing. This book was released on 2016-11-11 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monitoring and Evaluation of Biomaterials and Their Performance In Vivo provides essential information for scientists and researchers who need to assess and evaluate performance, monitor biological responses, gauge efficacy, and observe changes over time. Crucially, it also enables the optimization of design for future biomaterials and implants. This book presents readers with comprehensive coverage of the topic of in vivo monitoring of medical implants and biomaterials. Contains a specific focus on monitoring and evaluation of biomaterials in vivo Multi-faceted coverage of materials function and performance Focuses on a range of implants and subsequent bodily reactions

World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China

Author : Mian Long
Publisher : Springer Science & Business Media
ISBN 13 : 3642293050
Total Pages : 2385 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China by : Mian Long

Download or read book World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China written by Mian Long and published by Springer Science & Business Media. This book was released on 2013-02-11 with total page 2385 pages. Available in PDF, EPUB and Kindle. Book excerpt: The congress’s unique structure represents the two dimensions of technology and medicine: 13 themes on science and medical technologies intersect with five challenging main topics of medicine to create a maximum of synergy and integration of aspects on research, development and application. Each of the congress themes was chaired by two leading experts. The themes address specific topics of medicine and technology that provide multiple and excellent opportunities for exchanges.

Raman Spectroscopy In Human Health And Biomedicine

Author : Hidetoshi Sato
Publisher : World Scientific
ISBN 13 : 9811264627
Total Pages : 657 pages
Book Rating : 4.8/5 (112 download)

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Book Synopsis Raman Spectroscopy In Human Health And Biomedicine by : Hidetoshi Sato

Download or read book Raman Spectroscopy In Human Health And Biomedicine written by Hidetoshi Sato and published by World Scientific. This book was released on 2023-09-21 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the inelastic scattering of light was predicted nearly 100 years ago, Raman spectroscopy has become a mainstay of characterization techniques, with applications in a vast array of fields from chemistry to materials science and nanotechnology, from forensics to geology and art. More recently, it has found usage in the life sciences, and this book hereby outlines the state-of-the-art advances in applications of Raman spectroscopy to human health and biomedicine. It covers a wide range of human health science including medicine (especially cancer), physiology, biological molecules, pharmaceutical science, cells, viruses, microorganisms, and food science. Another highlight is that it describes recent progress on various Raman techniques such as surface-enhanced Raman scattering, tip-enhanced Raman scattering, non-linear Raman spectroscopy, Raman microscopy, and Raman imaging. Novel spectral analysis methods such as chemometrics are also prominently discussed.

Advances in Molecular Toxicology Vol 11

Author :
Publisher : Academic Press
ISBN 13 : 0128125233
Total Pages : 292 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Advances in Molecular Toxicology Vol 11 by :

Download or read book Advances in Molecular Toxicology Vol 11 written by and published by Academic Press. This book was released on 2017-09-15 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Molecular Toxicology, Volume 11, features the latest advances in the subspecialties of the broad area of molecular toxicology. This series details the study of the molecular basis of toxicology by which a vast array of agents encountered in the human environment, and produced by the human body, manifest themselves as toxins. The book is not strictly limited to documenting these examples, but also covers the complex web of chemical and biological events that give rise to toxin-induced symptoms and disease. The new technologies that are being harnessed to analyze and understand these events are also reviewed by leading experts in the field. Provides cutting-edge reviews by leading workers in the discipline Includes in-depth dissection of the molecular aspects that are of interest to a broad range of scientists, physicians and any student in the allied disciplines Presents leading-edge applications of technological innovations in chemistry, biochemistry and molecular medicine

AACR 2017 Proceedings: Abstracts 3063-5947

Author : American Association for Cancer Research
Publisher : CTI Meeting Technology
ISBN 13 :
Total Pages : 2975 pages
Book Rating : 4./5 ( download)

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Book Synopsis AACR 2017 Proceedings: Abstracts 3063-5947 by : American Association for Cancer Research

Download or read book AACR 2017 Proceedings: Abstracts 3063-5947 written by American Association for Cancer Research and published by CTI Meeting Technology. This book was released on 2017-03-13 with total page 2975 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Imaging

Author : Brian D. Ross
Publisher : Academic Press
ISBN 13 : 0128163879
Total Pages : 1872 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Molecular Imaging by : Brian D. Ross

Download or read book Molecular Imaging written by Brian D. Ross and published by Academic Press. This book was released on 2021-08-03 with total page 1872 pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection and measurement of the dynamic regulation and interactions of cells and proteins within the living cell are critical to the understanding of cellular biology and pathophysiology. The multidisciplinary field of molecular imaging of living subjects continues to expand with dramatic advances in chemistry, molecular biology, therapeutics, engineering, medical physics and biomedical applications. Molecular Imaging: Principles and Practice, Volumes 1 and 2, Second Edition provides the first point of entry for physicians, scientists, and practitioners. This authoritative reference book provides a comprehensible overview along with in-depth presentation of molecular imaging concepts, technologies and applications making it the foremost source for both established and new investigators, collaborators, students and anyone interested in this exciting and important field. The most authoritative and comprehensive resource available in the molecular-imaging field, written by over 170 of the leading scientists from around the world who have evaluated and summarized the most important methods, principles, technologies and data Concepts illustrated with over 600 color figures and molecular-imaging examples Chapters/topics include, artificial intelligence and machine learning, use of online social media, virtual and augmented reality, optogenetics, FDA regulatory process of imaging agents and devices, emerging instrumentation, MR elastography, MR fingerprinting, operational radiation safety, multiscale imaging and uses in drug development This edition is packed with innovative science, including theranostics, light sheet fluorescence microscopy, (LSFM), mass spectrometry imaging, combining in vitro and in vivo diagnostics, Raman imaging, along with molecular and functional imaging applications Valuable applications of molecular imaging in pediatrics, oncology, autoimmune, cardiovascular and CNS diseases are also presented This resource helps integrate diverse multidisciplinary concepts associated with molecular imaging to provide readers with an improved understanding of current and future applications

Frontiers in Anti-Cancer Drug Discovery Volume 10

Author : Atta-ur-Rahman
Publisher : Bentham Science Publishers
ISBN 13 : 9811401918
Total Pages : 252 pages
Book Rating : 4.8/5 (114 download)

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Book Synopsis Frontiers in Anti-Cancer Drug Discovery Volume 10 by : Atta-ur-Rahman

Download or read book Frontiers in Anti-Cancer Drug Discovery Volume 10 written by Atta-ur-Rahman and published by Bentham Science Publishers. This book was released on 2019-05-31 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Frontiers in Anti-Cancer Drug Discovery is a book series devoted to publishing the latest advances in anti-cancer drug design and discovery. In each volume, eminent scientists contribute reviews relevant to all areas of rational drug design and drug discovery including medicinal chemistry,in-silico drug design, combinatorial chemistry, high-throughput screening, drug targets, recent important patents, and structure-activity relationships. The book series should prove to be of interest to all pharmaceutical scientists involved in research in anti-cancer drug design and discovery. The book series is essential reading to all scientists involved in drug design and discovery who wish to keep abreast of rapid and important developments in the field. The tenth volume of the series features chapters covering the following topics: - Challenges in the Management of Hepatoblastoma - The Emerging Role of Monocarboxylate Transporter-1 in Cancer - In-vitro Anti-Proliferative Assays and Techniques Used in Pre-Clinical Anti-Cancer Drug Discovery - Recent Advances in the Development of Mesoporous Anti-Cancer Drug Nanocarriers - Polyphenols and Cancer - Glioblastoma Multiforme - Cutting Edge Targeting Strategies Utilizing Nanotechnology in Breast Cancer Therapy.

Surface and Interfacial Aspects of Cell Adhesion

Author : Alain Carre
Publisher : CRC Press
ISBN 13 : 9004190805
Total Pages : 546 pages
Book Rating : 4.0/5 (41 download)

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Book Synopsis Surface and Interfacial Aspects of Cell Adhesion by : Alain Carre

Download or read book Surface and Interfacial Aspects of Cell Adhesion written by Alain Carre and published by CRC Press. This book was released on 2011-01-19 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell adhesion comes into play in almost all domains of life. The range of situations in which it occurs, involving organisms, living tissues, microorganisms or single cells, is endless. Cell adhesion is involved in the binding of a cell to a surface, extracellular matrix, or another cell using cell adhesion molecules. It is crucial in the formation