Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Label Free Characterization Of The Biochemistry And Dynamics Of Individual Cells By Laser Tweezers Raman Spectroscopy
Download Label Free Characterization Of The Biochemistry And Dynamics Of Individual Cells By Laser Tweezers Raman Spectroscopy full books in PDF, epub, and Kindle. Read online Label Free Characterization Of The Biochemistry And Dynamics Of Individual Cells By Laser Tweezers Raman Spectroscopy ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Label-free Characterization of the Biochemistry and Dynamics of Individual Cells by Laser Tweezers Raman Spectroscopy by : Tobias Jan Moritz
Download or read book Label-free Characterization of the Biochemistry and Dynamics of Individual Cells by Laser Tweezers Raman Spectroscopy written by Tobias Jan Moritz and published by . This book was released on 2010 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development of Automated Analysis and Sorting of Single Cells Using Laser Tweezers Raman Spectroscopy by : Stephen Casabella
Download or read book Development of Automated Analysis and Sorting of Single Cells Using Laser Tweezers Raman Spectroscopy written by Stephen Casabella and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Optical Techniques in Regenerative Medicine by : Stephen P. Morgan
Download or read book Optical Techniques in Regenerative Medicine written by Stephen P. Morgan and published by Taylor & Francis. This book was released on 2016-04-19 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: In regenerative medicine, tissue engineers largely rely on destructive and time-consuming techniques that do not allow in situ and spatial monitoring of tissue growth. Furthermore, once the therapy is implanted in the patient, clinicians are often unable to monitor what is happening in the body. To tackle these barriers, optical techniques have bee
Book Synopsis Label-Free Monitoring of Cells in vitro by : Joachim Wegener
Download or read book Label-Free Monitoring of Cells in vitro written by Joachim Wegener and published by Springer Nature. This book was released on 2019-11-18 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.
Book Synopsis Laser Tweezers Raman Spectroscopy of Single Cells by : De Chen
Download or read book Laser Tweezers Raman Spectroscopy of Single Cells written by De Chen and published by . This book was released on 2008 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Label-Free Analysis of Drug Delivery Systems and Cellular Interaction Studies Using Confocal Raman Microscopy by : Birthe Kann
Download or read book Label-Free Analysis of Drug Delivery Systems and Cellular Interaction Studies Using Confocal Raman Microscopy written by Birthe Kann and published by Cuvillier Verlag. This book was released on 2016-08-12 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: In pharmaceutical development many questions still remain unsolved despite the availability of many analytical techniques. Consequently, the need of novel analytical approaches is not yet satisfied. In this thesis, confocal Raman microscopy is utilized to fill the scientific gap. In fact, the benefit of this non-destructive, label-free visualization technique for profound analysis in complex pharmaceutical applications is successfully demonstrated. The impact of drying on drug distribution is proven by localizing the drug in wet-extruded pellets with Raman imaging. Additionally to this finding, the correlation between drug distribution and release is successfully elucidated. For the first time, confocal Raman microscopy is combined with optical profilometry. Thus, the limitations of the confocal microscope are overcome and all-encompassing component visualization in complex drug delivery systems exhibiting challenging structured surfaces is realized. During development of a lipid-based drug permeation model, the successive formation of the permeation barrier during coating is finally described using Raman analysis. Investigations benefit tremendously from a combination of chemical imaging in lateral and vertical planes to depict barrier integrity and stability. Finally, human cells as well as the uptake of different nanoparticles are analyzed label-free in aqueous environment, utilizing linear and coherent Raman techniques.
Book Synopsis Imaging and Spectroscopic Analysis of Living Cells by :
Download or read book Imaging and Spectroscopic Analysis of Living Cells written by and published by Academic Press. This book was released on 2012-03-15 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Book Synopsis Dynamic Force Spectroscopy and Biomolecular Recognition by : Anna Rita Bizzarri
Download or read book Dynamic Force Spectroscopy and Biomolecular Recognition written by Anna Rita Bizzarri and published by CRC Press. This book was released on 2012-01-25 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies and is finding wider applications in areas such as biophysics and polymer science. In six chapters, Dynamic Force Spectroscopy and Biomolecular Recognition covers the most recent ideas and advances in the field of DFS applied to biorecognition: Chapter 1: Reviews the basic and novel aspects of biorecognition and discusses the emerging capabilities of single-molecule techniques to disclose kinetic properties and molecular mechanisms usually hidden in bulk measurements Chapter 2: Describes the basic principle of atomic force microsocopy (AFM) and DFS, with particular attention to instrumental and theoretical aspects more strictly related to the study of biomolecules Chapter 3: Overviews the theoretical background in which experimental data taken in nonequilibrum measurements of biomolecular unbinding forces are extrapolated to equilibrium conditions Chapter 4: Reviews the most common and efficient strategies adopted in DFS experiments to immobilize the interacting biomolecules to the AFM tip and to the substrate Chapter 5: Presents and discusses the most representative aspects related to the analysis of DFS data and the challenges of integrating well-defined criteria to calibrate data in automatic routinary procedures Chapter 6: Overviews the most relevant DFS applications to study biorecognition processes, including the biotin/avidin pair, and selected results on various biological complexes, including antigen/antibody, proteins/DNA, and complexes involved in adhesion processes Chapter 7: Summarizes the main results obtained by DFS applied to study biorecognition processes with forthcoming theoretical and experimental advances Although DFS is a widespread, worldwide technique, no books focused on this subject have been available until now. Dynamic Force Spectroscopy and Biomolecular Recognition provides the state of the art of experimental data analysis and theoretical procedures, making it a useful tool for researchers applying DFS to study biorecognition processes.
Book Synopsis Spectroscopy of Inorganic Bioactivators by : T. Theophanides
Download or read book Spectroscopy of Inorganic Bioactivators written by T. Theophanides and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition is the labor of many enthousiastic scientists who were invited to teach at a NATO Advanced Study Institute on the "Spectroscopy of Inorganic Bioactivators" which took place on August 20 -30, 1988 at Club Poseidon, Loutraki, Greece. In this book the subjects were taught through several well prepared lectures. These lectures stretch the fact that scientific knowledge is the painfully gathered product of many wonderful human minds. I made an attempt to divide the lectures into separate chapters, however, there is interaction among the lectures, as I hope the book will show. First, there is introductory on an lecture supercomputing and super computers and their applications to solving structures of biological molecules followed by a state-of-the-art x-ray diffraction method at pi co second times. Important new advances have been made in x-ray diffraction analysis at picosecond times, in Hadamard spectroscopy, in micro-Raman spectroscopy in the Near Infrared region (1. 01 ~m) and remote sensing by Fourier Transform Infrared Spectroscopy. The chapters that follow include applications of spectroscopic technique to vii viii biologically important molecules, such as, DNA, proteins, membranes, and metal ion-biological molecule interactions. I would like to express my thanks to all the authors for their contributions and their cooperation in submitting their manuscript. I also thank the NATO Science Committee for making this possible. The field looks very promising for significant and exciting developments in the application of spectroscopy to bioactivators.
Download or read book Biophotonics written by Matthew Baker and published by Morgan & Claypool Publishers. This book was released on 2016-02-01 with total page 93 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophotonic diagnostics/biomedical spectroscopy can revolutionise the medical environment by providing a responsive and objective diagnostic environment. This book aims to explain the fundamentals of the physical techniques used combined with the particular requirements of analysing medical/clinical samples as a resource for any interested party. In addition, it will show the potential of this field for the future of medical science and act as a driver for translation across many different biological problems/questions.
Book Synopsis Imaging and Spectroscopic Analysis of Living Cells by : P. Michael Conn
Download or read book Imaging and Spectroscopic Analysis of Living Cells written by P. Michael Conn and published by Academic Press. This book was released on 2012 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Publisher's note.
Book Synopsis Neurophotonics and Biomedical Spectroscopy by : Robert R. Alfano
Download or read book Neurophotonics and Biomedical Spectroscopy written by Robert R. Alfano and published by William Andrew. This book was released on 2018-11-22 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neurophotonics and Biomedical Spectroscopy addresses the novel state-of-the-art work in non-invasive optical spectroscopic methods that detect the onset and progression of diseases and other conditions, including pre-malignancy, cancer, Alzheimer’s disease, tissue and cell response to therapeutic intervention, unintended injury and laser energy deposition. The book then highlights research in neurophotonics that investigates single and multi-photon excitation optical signatures of normal/diseased nerve tissues and in the brain, providing a better understanding of the underlying biochemical and structural changes of tissues and cells that are responsible for the observed spectroscopic signatures. Topics cover a wide array of well-established UV, visible, NIR and IR optical and spectroscopic techniques and novel approaches to diagnose tissue changes, including: label free in vivo and ex vivo fluorescence spectroscopy, Stoke shift spectroscopy, spectral imaging, Resonance Raman spectroscopy, multiphoton two Photon excitation, and more. Provides an overview of the spectroscopic properties of tissue and tissue-light interaction, describing techniques to exploit these properties in imaging Explores the potential and significance of molecule-specific imaging and its capacity to reveal vital new information on nanoscale structures Offers a concise overview of different spectroscopic methods and their potential benefits for solving diagnostic and therapeutic problems
Book Synopsis Imaging and Spectroscopic Analysis of Living Cells by : P. Michael Conn
Download or read book Imaging and Spectroscopic Analysis of Living Cells written by P. Michael Conn and published by Academic Press. This book was released on 2012-01-24 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Book Synopsis Dynamic Studies in Biology by : Maurice Goeldner
Download or read book Dynamic Studies in Biology written by Maurice Goeldner and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers. A must-have for every biochemist, biophysicist and molecular biologist developing and working with these novel methods.
Download or read book Biophotonics, Part B written by and published by Elsevier. This book was released on 2003-03-11 with total page 627 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume and its companion volume 360 introduce a new topic to the Methods in Enzymology series. They will cover, among other topics, imaging, screening, and diagnosis in biological systems. See key features for greater detail. Key Features* Optical instrumentation for imaging, screening and diagnosis in molecules, tissues, and cells* Development and application of optical probes and techniques for imaging and drug screening, protemics, genomics, and cellomics* Applications of biophotonics research to the understanding of mechanisms of cellular reactions and processes, investigating the structure and dynamics of biomolecular systems, screening and drug discovery, and diagnosis and treatment of disease
Book Synopsis Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths by : Ran Li
Download or read book Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths written by Ran Li and published by . This book was released on 2013 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: In recent years, Raman spectroscopy has emerged as a potentially useful tool for the detection and pathological diagnosis of cancerous tissues. This technique has the merit of being highly sensitive to the biochemical alterations in tissue compositions. Nevertheless, the use of Raman spectroscopy in cancer detection has been impeded by high background intensity which can mask the Raman signal of biological molecules. In this study, we aimed at investigating the origins of background and reducing it by studying the influence of both excitation wavelengths and optical modes. Different optical modes, single mode and multimode lasers, with the same excitation 785 nm and different excitation wavelengths, 785 nm and 830 nm lasers with the same multimode are compared here. According to this study, autofluorescence and multiple Mie scattering give rise to the high background intensities in the Raman spectra. The reduction in autofluorescence is demonstrated in the comparison between 785 nm multimode (785 MM) and 830 nm multimode (830 MM) lasers. 830 MM demonstrates better autofluorescence reduction ability than 785 MM. However, the Raman signal intensity is subsequently reduced by 830 MM. Therefore, the SNRs are not improved in spectra taken by 830 MM than by 785 MM. The reduction in multiple Mie scattering is demonstrated in the comparison between 785 MM and 785 nm single mode (785 SM). 785 SM demonstrates better Mie scattering reduction ability than 785 MM, with equal Raman signal intensities.
Book Synopsis Single Cell Analysis by : J. Paul Robinson
Download or read book Single Cell Analysis written by J. Paul Robinson and published by Springer. This book was released on 2017-04-18 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings together an eclectic group of current applications, all of which have a significant impact on our current state of knowledge. The authors of these chapters are all pioneering researchers in the field of single cell analysis. The book will not only appeal to those readers more focused on clinical applications, but also those interested in highly technical aspects of the technologies. All of the technologies identified utilize unique applications of photon detection systems.
