Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Investigations Into The Chemistry Of Protein Tyrosine Phosphatase Redox Regulation
Download Investigations Into The Chemistry Of Protein Tyrosine Phosphatase Redox Regulation full books in PDF, epub, and Kindle. Read online Investigations Into The Chemistry Of Protein Tyrosine Phosphatase Redox Regulation ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Investigations Into the Chemistry of Protein Tyrosine Phosphatase Redox Regulation by : Jason N. LaButti
Download or read book Investigations Into the Chemistry of Protein Tyrosine Phosphatase Redox Regulation written by Jason N. LaButti and published by . This book was released on 2009 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transmission of complex intracellular signals, such as those for glucose uptake or proliferation, is often accomplished through the reversible phosphorylation of specific protein tyrosine residues. This reversible phosphorylation serves as a biochemical "rheostat" that alters a protein's functional properties and leads to propagation of the signal. The phosphorylation status of these tyrosine residues, thus transmission of the cellular signal itself, is tightly controlled by the opposing actions of protein tyrosine kinases that catalyze the addition of phosphoryl groups and protein tyrosine phosphatases (PTPs) are cysteine based enzymes that catalyze their removal. Abstraction of these phosphoryl groups, in many cases, serves as an "off switch" to terminate the cellular responses to the extracellular stimulus. PTPs, therefore, play a central role in the regulation of diverse cellular processes including glucose metabolism, cell cycle control and immune responses. Accordingly, small molecules capable of inactivating PTPs through reversible oxidation of their active site cysteine thiolate may find use as therapeutic agents and/or tools for the study of diverse signal transduction pathways. In the body of work presented here we report the chemical properties of a novel PTP redox regulator and develop new methodologies for studying PTP redox regulation.
Book Synopsis Protein Tyrosine Phosphatases—Advances in Research and Application: 2012 Edition by :
Download or read book Protein Tyrosine Phosphatases—Advances in Research and Application: 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Tyrosine Phosphatases—Advances in Research and Application: 2012 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Protein Tyrosine Phosphatases in a compact format. The editors have built Protein Tyrosine Phosphatases—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Protein Tyrosine Phosphatases in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Protein Tyrosine Phosphatases—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Book Synopsis Expanding the Chemical Tool Kit for the Detection of Redox Regulated Protein Tyrosine Phosphatases by : Francisco Javier Garcia
Download or read book Expanding the Chemical Tool Kit for the Detection of Redox Regulated Protein Tyrosine Phosphatases written by Francisco Javier Garcia and published by . This book was released on 2016 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Tyrosine Phosphatases by : Damien Thévenin
Download or read book Protein Tyrosine Phosphatases written by Damien Thévenin and published by Springer Nature. This book was released on 2024-01-27 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. Furthermore, new advances in pharmacology and drug design have contributed to the developing novel therapeutics that target PTPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein Tyrosine Phosphatases: Methods and Protocols, Second Edition is a valuable resource for both experienced and novel researchers in this field, and will lead to discoveries and accelerated progress in the field of PTP, signal transduction, and drug development.
Book Synopsis Kinetics and Mechanisms Associated with Redox Regulation of Protein Tyrosine Phosphatase 1B (PTP1B) by : Zachary D. Parsons
Download or read book Kinetics and Mechanisms Associated with Redox Regulation of Protein Tyrosine Phosphatase 1B (PTP1B) written by Zachary D. Parsons and published by . This book was released on 2014 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, we report on the kinetics and mechanisms associated with redox regulation of the protein tyrosine phosphatase PTP1B. We determined rates of thiol- and phosphine-mediated recovery of catalytic activity from the oxidatively-inactivated enzyme, and shed light on the chemical mechanisms involved in these processes and in the regulation of PTP family member SHP2. Additionally, we describe reactions that oxidativelyinactivated PTP1B undergo with structurally-diverse carbon acids, using both a small chemical model dipeptide of oxidatively-inactivated PTP1B, and the oxidized enzyme itself. Finally, we report a novel method by which to predict Hammett substituent constants via electronic structure calculations.
Book Synopsis Protein Tyrosine Phosphatase Control of Metabolism by : Kendra K. Bence
Download or read book Protein Tyrosine Phosphatase Control of Metabolism written by Kendra K. Bence and published by Springer Science & Business Media. This book was released on 2013-08-30 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although phosphorylation of proteins on tyrosine is relatively rare compared to phosphorylation on serine or threonine residues, the past two decades of research into PTP function have led to a great appreciation of the critical role PTPs have in regulating basic cellular processes. Among these important roles is the regulation of cellular signaling pathways related to metabolism. This volume contains chapters which highlight many aspects of PTP function in the context of metabolism. Given the growing obesity and diabetes epidemics in the United States and throughout the world, the desire to identify possible therapeutic targets for treatment of these diseases is a high priority. In many ways, PTPs may be attractive drug targets since they are amenable to targeting with small molecules; however many challenges abound in making PTP inhibitors.
Book Synopsis Redox Biochemistry by : Ruma Banerjee
Download or read book Redox Biochemistry written by Ruma Banerjee and published by John Wiley & Sons. This book was released on 2007-12-04 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the premier, single-source reference on redox biochemistry, a rapidly emerging field. This reference presents the basic principles and includes detailed chapters focusing on various aspects of five primary areas of redox biochemistry: antioxidant molecules and redox cofactors; antioxidant enzymes; redox regulation of physiological processes; pathological processes related to redox; and specialized methods. This is a go-to resource for professionals in pharmaceuticals, medicine, immunology, nutrition, and environmental fields and an excellent text for upper-level students.
Book Synopsis Specific and Reversible Inactivation of Protein Tyrosine Phosphatases by Hydrogen Peroxide by : John M. Denu
Download or read book Specific and Reversible Inactivation of Protein Tyrosine Phosphatases by Hydrogen Peroxide written by John M. Denu and published by . This book was released on 1998 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biologically Relevant Chemistry of Sulfur Heterocycles by : Santhosh Sivaramakrishnan
Download or read book Biologically Relevant Chemistry of Sulfur Heterocycles written by Santhosh Sivaramakrishnan and published by . This book was released on 2008 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein tyrosine phosphatase 1B (PTP1B) is an important member of protein tyrosine phosphatase (PTPs) family that shares a conserved catalytic cysteine thiol residue. PTP1B acts as a negative regulator of insulin mediated signaling cascade, as inhibition of PTP1B is shown to increase the insulin sensitivity. A key feature of signal transduction pathway involves transient inactivation of PTP1B by an insulin stimulated burst of hydrogen peroxide. The catalytic cysteine thiol of PTP1B undergoes oxidation in presence of hydrogen peroxide to generate an inactive sulfenic acid, which is reactivated by the action of cellular thiols and reducing enzymes. However, recent evidences suggest an unique chemical transformation to generate sulfenyl amide (3-isothiazolidinone) from sulfenic acid at the active site of PTP1B. The mechanism for the formation of this novel sulfenyl amide is not clear. We employed 1,2-benzisothiazolidinone as chemical model to mimic the important functional groups present at the active site of enzyme and examined the chemical mechanism leading to the formation of sulfenyl amide. Our results supported a direct intramolecular attack of neighboring amide nitrogen onto the sulfenic acid to generate sulfenyl amide. Generation of the novel sulfenyl amide heterocycle is believed to protect the enzyme against irreversible over-oxidation to sulfinic and sulfonic acids. However, as the claims lacked a strong experimental support, we used our chemical models to estimate the rate constant for the hydrogen peroxide-mediated over-oxidation of sulfenyl amide. Our results suggested that sulfenyl amide does in fact resist irreversible over-oxidation compared to a sulfenic acid surrogate. Furthermore, we developed a method to generate sulfenyl amide during the hydrogen peroxide-mediated oxidation of PTP1B in aqueous buffer solution. In addition, we determined the rate constant for the formation and over-oxidation of sulfenyl amide at the active site of PTP1B. We also utilized our model compounds to examine the chemical properties of "overoxidized" intermediates. Our results suggested that over-oxidation of sulfenyl amide will generate a thiol reversible but hydrolytically labile sulfinyl amide. We extended our knowledge gained from the model studies and investigated the formation of sulfinyl amide during oxidative inactivation of PTP1B. Consistent with our expectations, we were successful in identifying a thiol reversible but hydrolytically labile intermediate at the active site of PTP1B. Furthermore, we determined the rate constant for the hydrolysis of sulfinyl amide formed in PTP1B. Overall, our findings provided first evidence for the formation of this novel thiol-reversible sulfinyl amide heterocycle during the redox regulation of PTP1B. In addition, our results suggest that formation of sulfinyl amide may protect the enzyme against irreversible over-oxidation. In a different context, we examined possible chemical mechanisms underlying the biological activity of natural product S-deoxy leinamycin. We synthesized a crucial triggering unit 1,2-dithiolan 3-one moiety of the natural product and investigated the generation of reactive oxygen species using DNA cleavage assays. Our results suggested that the reaction of 1,2- dithiolan 3-one heterocycle found in S-deoxy leinamycin with thiols leads to the generation of persulfide intermediate (RSS- ). The persulfide intermediate then generates biologically active polysulfides, hydrogen sulfide and reactive oxygen species that may ultimately explain the biological activity of S-deoxy leinamycin.
Book Synopsis Hydrogen Peroxide and Cell Signaling, Part B by :
Download or read book Hydrogen Peroxide and Cell Signaling, Part B written by and published by Academic Press. This book was released on 2013-07-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling.
Book Synopsis Redox Regulation of Protein Tyrosine Phosphatases in Cell Membrane Receptor-mediated Signal Transduction by : Scott Joseph Salsman
Download or read book Redox Regulation of Protein Tyrosine Phosphatases in Cell Membrane Receptor-mediated Signal Transduction written by Scott Joseph Salsman and published by . This book was released on 2005 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Investigations of Protein Tyrosine Phosphatase Functions by :
Download or read book Investigations of Protein Tyrosine Phosphatase Functions written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The reversible modification of cellular proteins by tyrosine phosphorylation is a central mechanism of signal transduction, which controls cell physiological processes, such as proliferation, migration or differentiation in health and disease. Protein tyrosine kinases (PTKs) and -phosphatases (PTPs) regulate these phosphorylation events in an antagonistic manner. In this thesis the regulation of the receptor protein PTPRK by proteolytic processing was investigated. Thereby, a novel mechanism of PTP regulation was identified, which allows the relocalization of this receptor protein from the cell surface to the nucleus. In a second project, methods and strategies were developed to elucidate the substrate specificity of PTPs with quantitative mass spectrometry. Using this approach it was possible to define a large number of new potential substrate proteins for the phosphatase PTP1B.
Book Synopsis Handbook of Cell Signaling by : Ralph A. Bradshaw
Download or read book Handbook of Cell Signaling written by Ralph A. Bradshaw and published by Academic Press. This book was released on 2009-11-03 with total page 3188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Cell Signaling, Three-Volume Set, 2e, is a comprehensive work covering all aspects of intracellular signal processing, including extra/intracellular membrane receptors, signal transduction, gene expression/translation, and cellular/organotypic signal responses. The second edition is an up-to-date, expanded reference with each section edited by a recognized expert in the field. Tabular and well illustrated, the Handbook will serve as an in-depth reference for this complex and evolving field. Handbook of Cell Signaling, 2/e will appeal to a broad, cross-disciplinary audience interested in the structure, biochemistry, molecular biology and pathology of cellular effectors. Contains over 350 chapters of comprehensive coverage on cell signaling Includes discussion on topics from ligand/receptor interactions to organ/organism responses Provides user-friendly, well-illustrated, reputable content by experts in the field
Book Synopsis Protein Tyrosine Phosphatases in Cancer by : Benjamin G. Neel
Download or read book Protein Tyrosine Phosphatases in Cancer written by Benjamin G. Neel and published by Springer. This book was released on 2016-08-05 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to bridge the gap in understanding how protein-tyrosine phosphatases (PTPs), which carry out the reverse reaction of tyrosine phosphorylation, feature in cancer cell biology. The expertly authored chapters will first review the general features of the PTP superfamily, including their overall structure and enzymological properties; use selected examples of individual PTP superfamily members, to illustrate emerging data on the role of PTPs in cancer; and will review the current status of PTP-based drug development efforts. Protein Tyrosine Phosphatases in Cancer,from renowned researchers Benjamin Neel and Nicholas Tonks, is invaluable reading for researchers in oncology, stem cell signaling,and biochemistry.
Book Synopsis Redox Signaling and Biomarkers in Ageing by : Ufuk Çakatay
Download or read book Redox Signaling and Biomarkers in Ageing written by Ufuk Çakatay and published by Springer Nature. This book was released on 2021-12-02 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to present the age-related alterations in redox signaling networks and their diagnostic biomarkers in aging cells using multidisciplinary approach. Establishing sensitive and specific biomarkers of dynamic redox homeostasis is crucially important in the development of effective antiaging and senolytic interventions. Recent years have seen tremendous advances in the understanding of redox signaling events which highlight the process of aging and age-related pathologies. A major challenge in biological aging research is developing reliable biomarkers to determine the consequences of disrupted redox signaling networks long before the clinical diagnosis of age-related diseases is made. Therefore, we have chosen to concentrate on aging-induced aberrant redox signaling networks, their biomarkers, and pathological consequences in this book. Although oxidation is a natural metabolic process, the imbalance in the level of oxidants and antioxidants causes oxidative stress and eventually leads to inflammatory conditions, diabetes, neurodegenerative diseases, and cancer. Novel redox-sensitive biomarkers for the evaluation of aging-induced proteinopathies such as amyloid ß and tau proteins in Alzheimer's disease, α-synuclein in Parkinson's disease, and islet amyloid polypeptides in type 2 diabetes mellitus recently drew the attention of researchers. Inside this textbook, readers will find comprehensive perspectives on the association between redox homeostasis and the aging process both at the molecular and clinical levels. Due to the inherent relationship between impaired metabolic activities and oxidative stress, the temporal interaction between intermediary metabolism and disturbed redox status can lead to greater susceptibility to aging-induced diseases and disorders, such as cardiovascular diseases, hypertension, and diabetes. This knowledge could be a key to continued research toward improving medication regimens such as in cancer and cardiovascular therapies, and procedural outcomes for patients. This book brings together current research evidence and knowledge on redox signaling and biomarkers in aging in chapters written by leading global experts in this rapidly evolving field. We hope that this textbook is of interest to a wide group of researchers, advanced students, scientifically curious non-specialist readers and clinicians alike.
Book Synopsis Innovative Medicine by : Kazuwa Nakao
Download or read book Innovative Medicine written by Kazuwa Nakao and published by Springer. This book was released on 2015-10-13 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is devoted to innovative medicine, comprising the proceedings of the Uehara Memorial Foundation Symposium 2014. It remains extremely rare for the findings of basic research to be developed into clinical applications, and it takes a long time for the process to be achieved. The task of advancing the development of basic research into clinical reality lies with translational science, yet the field seems to struggle to find a way to move forward. To create innovative medical technology, many steps need to be taken: development and analysis of optimal animal models of human diseases, elucidation of genomic and epidemiological data, and establishment of “proof of concept”. There is also considerable demand for progress in drug research, new surgical procedures, and new clinical devices and equipment. While the original research target may be rare diseases, it is also important to apply those findings more broadly to common diseases. The book covers a wide range of topics and is organized into three complementary parts. The first part is basic research for innovative medicine, the second is translational research for innovative medicine, and the third is new technology for innovative medicine. This book helps to understand innovative medicine and to make progress in its realization.
Book Synopsis Oxidative Stress and Redox Regulation in Plants by :
Download or read book Oxidative Stress and Redox Regulation in Plants written by and published by Academic Press. This book was released on 2009-10-09 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of redox is rapidly changing, specifically in relation to plants where redox reactions are exacerbated compared to non-photosynthetic organisms. The development of proteomics has allowed the identification of hundreds of molecular targets of these systems, and the recent discovery of glutaredoxin's ability to bind iron sulfur centers (ISCs) and to participate in ISC assembly in other apoproteins has provided many new insights. This volume presents new research on oxidative stress in plants, ranging from the production of reactive oxygen species or reactive nitrogen species, to their accumulation, their involvement in signal transduction, and their degradation, while also covering the links among oxidative stress and biotic and abiotic stresses. Cutting-edge reviews written from a broad range of scientific perspectives For over 40 years, series has enjoyed a reputation for excellence Contributors internationally recognized authorities in their respective fields
