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Interaction Of Ecoli Rna Polymerase With Phage Lambda Promoters
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Book Synopsis Interaction of E.coli RNA Polymerase with Phage [lambda] Promoters by : Elizabeth Margaret Owens
Download or read book Interaction of E.coli RNA Polymerase with Phage [lambda] Promoters written by Elizabeth Margaret Owens and published by . This book was released on 1979 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Interaction of Escherichia Coli RNA Polymerase with Phage [lambda] Promoters by : Jung-Hye Roe
Download or read book The Interaction of Escherichia Coli RNA Polymerase with Phage [lambda] Promoters written by Jung-Hye Roe and published by . This book was released on 1984 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Interaction of E. Coli RNA Polymerase with Lambda Phage PR Promoter by : Won-Chul Suh
Download or read book The Interaction of E. Coli RNA Polymerase with Lambda Phage PR Promoter written by Won-Chul Suh and published by . This book was released on 1993 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Mechanism of Interaction of E. Coli RNA Polymerase with Bacteriophage and Bacterial Promoters by : Sigrid Leirmo
Download or read book The Mechanism of Interaction of E. Coli RNA Polymerase with Bacteriophage and Bacterial Promoters written by Sigrid Leirmo and published by . This book was released on 1989 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Interactions Between Phage T7 Early Promoters and E. Coli RNA Polymerase by : Tao-shih Hsieh
Download or read book Interactions Between Phage T7 Early Promoters and E. Coli RNA Polymerase written by Tao-shih Hsieh and published by . This book was released on 1976 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Interaction of Bacillus Subtilis RNA Polymerase and Phage Ø29 Promoters by : Cynthia Dianne Dickel
Download or read book Interaction of Bacillus Subtilis RNA Polymerase and Phage Ø29 Promoters written by Cynthia Dianne Dickel and published by . This book was released on 1980 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Interaction of Base-analog Subsituted Lambda PR̳ Promoters with E. Coli RNA Polymerase by : John Woods Dubendorff
Download or read book The Interaction of Base-analog Subsituted Lambda PR̳ Promoters with E. Coli RNA Polymerase written by John Woods Dubendorff and published by . This book was released on 1985 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies on the Interaction of E. Coli RNA Polymerase with Lactose Promoter DNA by : Donald Duane Lorimer
Download or read book Studies on the Interaction of E. Coli RNA Polymerase with Lactose Promoter DNA written by Donald Duane Lorimer and published by . This book was released on 1989 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Interaction Between Phage Functions and E. Coli RNA Polymerase in the Control of Transcription in by : Siobhan Yeats
Download or read book Interaction Between Phage Functions and E. Coli RNA Polymerase in the Control of Transcription in written by Siobhan Yeats and published by . This book was released on 1980 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Selective Binding of E. Coli RNA Polymerase to Promoters on Phage T7 DNA by : Harlee S. Strauss
Download or read book The Selective Binding of E. Coli RNA Polymerase to Promoters on Phage T7 DNA written by Harlee S. Strauss and published by . This book was released on 1979 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Interaction of Escherichia Coli RNA Polymerase Holoenzyme Containing Sigma 32 with Promoters for Heat Shock Genes by : Deborah Walker Cowing
Download or read book Interaction of Escherichia Coli RNA Polymerase Holoenzyme Containing Sigma 32 with Promoters for Heat Shock Genes written by Deborah Walker Cowing and published by . This book was released on 1988 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Study of the Interaction of E. Coli RNA Polymerase and Bacteriophage S13 DNA by : Rassart, Eric
Download or read book A Study of the Interaction of E. Coli RNA Polymerase and Bacteriophage S13 DNA written by Rassart, Eric and published by 1977.. This book was released on 1977 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Study of the Interaction of E. Coli RNA Polymerase and Bacteriophage S13 DNA by : Eric Rassart
Download or read book A Study of the Interaction of E. Coli RNA Polymerase and Bacteriophage S13 DNA written by Eric Rassart and published by . This book was released on 1977 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Kinetic Investigation of the Molecular Processes Involved in the Mechanism of Open Complex Formation Between E. Coli RNA Polymerase and the [lambda]Pr Promoter by : Wayne Kontur
Download or read book Kinetic Investigation of the Molecular Processes Involved in the Mechanism of Open Complex Formation Between E. Coli RNA Polymerase and the [lambda]Pr Promoter written by Wayne Kontur and published by . This book was released on 2006 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Kinetics and Mechanism of Open Complex Formation, Stabilization, and Initiation by E. Coli RNA Polymerase at [lambda]PR and T7A1 Promoters by : Hao-Che Wang
Download or read book Kinetics and Mechanism of Open Complex Formation, Stabilization, and Initiation by E. Coli RNA Polymerase at [lambda]PR and T7A1 Promoters written by Hao-Che Wang and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The flow of genetic information in cells starts with transcription by the enzyme RNA polymerase (RNAP) of DNA information to synthesize a wide variety of structural and functional RNAs which collectively participate in most cellular processes. Transcription is therefore a highly regulated step in gene expression. Research discussed here focuses on transcription initiation, the earliest phase of transcription, and on questions of how differences in promoter DNA sequences and length affect initiation thermodynamics and kinetics. During transcription initiation, RNAP binds to and unwinds the transcription start site region of promoter DNA, opening 13 bp to form an open complex (OC). Chapter 2 reports a detailed kinetic-mechanistic comparison of OC formation for the model λPR and T7A1 promoters, and Chapter 3 does the same for OC dissociation kinetics and mechanisms. For OC formation, kinetics of 2-amino purine (-4) fluorescence, Cy3 (-100) - Cy5 (+14) FRET, and single-dye PIFE were determined and compared with filter binding kinetics of formation of long-lived promoter complexes. A range of temperatures and of glycine betaine concentrations was examined. From these comparisons, we conclude that early steps in the mechanism of OC formation are similar for the two promoters while later steps including DNA opening differ significantly. Our data are consistent with previous reports that opening of the T7A1 bubble occurs in two adjacent kinetic steps vs. one step for λPR. After the DNA opening transition state, the kinetics and mechanisms of OC formation at these two promoters become similar again. In Chapter 3, we investigated the roles of clamp closing and of allosteric effects of discriminator and upstream interactions on downstream elements in stabilizing E. coli RNA polymerase- λPR promoter open complexes (OC). Our approach is to determine dissociation rate constants for the stable OC (kd) and for the unstable I2 intermediate OC (k-2) at λPR and T7A1 promoters and promoter variants with exchanged discriminators, core promoters or UP elements by filter binding assays. Values of kd are also determined for downstream-truncated promoters (at +12, +6) and for a jaw deletion RNAP variant. Analysis yields free energy changes 8́6G3^o for the conversion of unstable I2 to a stable OC, and the large contribution to 8́6G3^o for all promoters, from clamp closing on the promoter from +6 upstream, as well as the significant contributions to 8́6G3^o for promoters with the λPR discriminator from downstream interactions with the Îø lobe and Îø' jaw that are allosterically controlled by interactions of the discriminator with ϳ1.2 and the Îø gate loop. Lastly, in chapter 4, we further test how promoters with different OC stability affect the escaping of the RNAP from promoter. Preliminary results indicate the OC lifetime mainly alters the rate for converting the OC into initial complex (IC) for NTP incorporations. Taken together, our studies provide a more complete understanding of how promoter sequence and length influences different phases of transcription initiation.
Book Synopsis The Bacteriophage Lambda by : Alfred Day Hershey
Download or read book The Bacteriophage Lambda written by Alfred Day Hershey and published by . This book was released on 1971 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of Transcription of the Gene for the Sigma Subunit of E. Coli RNA Polymerase by a Heat Shock Promoter by : Wayne Emery Taylor
Download or read book Regulation of Transcription of the Gene for the Sigma Subunit of E. Coli RNA Polymerase by a Heat Shock Promoter written by Wayne Emery Taylor and published by . This book was released on 1983 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: