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Integrated Virus Detection
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Book Synopsis Integrated Virus Detection by : Charles H. Wick
Download or read book Integrated Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2014-11-13 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multiple viruses can be detected concurrently using the Integrated Virus Detection System (IVDS). Integrated Virus Detection describes this technology and provides many examples of applications including a chapter on viruses found in honeybees with descriptions of seasonal and yearly variation. This straightforward technology can be used to detect known, unknown, and unsequenced viruses collected from environmental and other complex biological sources. This book summarizes more than ten US patents issued for the invention of the IVDS, which is the common name of the electrospray–differential mobility analyzer method. The IVDS is powering mankind’s ability to rapidly detect, measure, and monitor viruses as well as virus-like particles. Three facts make rapid detection possible: virus size, which ranges from 20 to 800 nm.; disparity in each virus species’ particle size thus allowing size data to be used for detection and preliminary identification; and the fact that virus particle density is distinct from other nanoparticles. The IVDS can ascertain the absence of virion particles, thus presenting compelling evidence of a true negative, which is useful in verifying decontamination and other processes. In addition, large numbers of samples may be processed in an automated fashion, providing an excellent means to prescreen them for judicious targeting of subsequent tests such as PCR or the discriminating method for identifying microbes, which is mass spectrometry proteomics.* The book is helpful to anyone interested in virus detection, especially in situations where many viral types may coexist. *Identifying Microbes by Mass Spectrometry Proteomics (CRC Press 2013)
Download or read book Das Würzburger Glacis written by and published by . This book was released on 1964 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of the Physical Behavior of Viruses Using the Integrated Virus Detection System (IVDS). by :
Download or read book Analysis of the Physical Behavior of Viruses Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2004 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new method for virus analysis has been indicated for years. This situation has reached a new and emerging viruses that tax present detection methods. Adding the cost of current methods to analyze large numbers of samples and a clear and urgent situation has developed. Fortunately, a new means for detecting the present or absence of viruses has been developed that capitalizes on the fundamental physical properties associated with these tiny microbes. The Integrated Virus Detection System (IVDS) utilizes exquisite, but regular methods, to purify and concentrate samples for sizing and counting using the usual methods of Differential Mobility Analysis and Condensation Particle Counting. Thus a new method that is essentially a virus particle counter has been invented, patented and demonstrated for a wide range of both enveloped and non-enveloped viruses. The invention is described and examples illustrated.
Book Synopsis Characterization of the Integrated Virus Detection System (IVDS): A Novel Instrument for Virus Identification and Its Use in the Initial Characterization of a New Marine Virus by :
Download or read book Characterization of the Integrated Virus Detection System (IVDS): A Novel Instrument for Virus Identification and Its Use in the Initial Characterization of a New Marine Virus written by and published by . This book was released on 2004 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detection and quantification of unknown or novel virus samples remains one of the great technical challenges in biological sciences. Here we report the characterization of an instrument, The Integrated Virus Detection System (IVDS) as an accurate and rapid means to size and quantify viral unknowns.
Book Synopsis Characterization of the Integrated Virus Detection System (IVDS) Using MS-2 Bacteriophage by :
Download or read book Characterization of the Integrated Virus Detection System (IVDS) Using MS-2 Bacteriophage written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection and analysis of viruses have been goals of science for more than 70 years, following the first real evidence that a new type of microorganism was responsible for diseases in man and animals. These new microbes were smaller than bacteria, which have now been well documented, classified, and studied. Their small size made classifying the new microbes more difficult, and the field of virology has been advanced by biochemical techniques rather than by direct examination. Advancements in electron microscopy in more recent times have made advances in this area, and much has been reported on the physical features of more than 21 virus families. All these historic techniques are time consuming and require special knowledge and specialized chemicals and preparations to be successful. Capitalizing on the physical characteristics, it was possible to separate the families and count the individual viruses in a new and dramatic way, using easily obtained materials and simple-to-operate techniques. The only materials used in the Integrated Virus Detection System (IVDS) are a buffer, CO2 gas, and butanol. The work in this report presents the advances in the concentration and counting phases of IVDS. Results indicate a practical, easy to use device, which can count viruses in near real time. The JVDS can, in its final configuration, be expected to analyze a sample, count the viruses present, and give a preliminary identification for all viruses.
Book Synopsis Water Sample Analysis With the Integrated Virus Detection System by :
Download or read book Water Sample Analysis With the Integrated Virus Detection System written by and published by . This book was released on 2010 with total page 23 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study examined using the Integrated Virus Detection System (IVDS) for rapid monitoring and identification of viruses in various water samples. Initial protocols for determining the presence of viruses in the various waters were explored with the IVDS. The IVDS is a generic virus detector that only requires general reagents. The equipment allows for detection and testing of large volumes and multiple samples of water in a short period of time.
Book Synopsis Characterization of MS2 Bacteriophage by Integrated Virus Detection System (IVDS) Physical Counting Methodology by :
Download or read book Characterization of MS2 Bacteriophage by Integrated Virus Detection System (IVDS) Physical Counting Methodology written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new physically based methodology - The Integrated Virus Detection System (IVDS) - was used to characterize a high concentration, 10.2 mg protein/ml, sample preparation of MS2 Bacteriophage with a reported 10(exp 14) pfu/ml (DPM14) virus count in a common TNME buffer. Virus counts were made using the IVDS instrument following serial dilution. Results indicated virus counts of 1.5 x 10(exp 5) for the neat sample (DPM14), followed by 6.5 x 10(exp 4) viruses (DPM13), 1.2 x 10(exp 4) viruses (DPM12), 9.3 x 10(exp 2) viruses (DPM11), 88 viruses (DPM10), and 5 viruses (DPM9), respectively. Lower concentrations display a consistent multiplier and were consistent with target dilutions. Increases in virus concentration appear to decrease the multiplier. Variation is considered to be due to aggregation. Results demonstrate a consistent and simple-to-use methodology. Results further indicate that the IVDS instrument can be used for characterization of other virus preparations with equal ease and similar results.
Book Synopsis Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage by :
Download or read book Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage written by and published by . This book was released on 2005 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report characterizes the stability of the electrospray module of the IVDS system while analyzing a sample of MS2 bacteriophage. The electrospray parameters were varied across the stable range, and resultant output from the detector was analyzed.
Book Synopsis Mass Spectrometry and Integrated Virus Detection System Characterization of MS2 Bacteriophage by :
Download or read book Mass Spectrometry and Integrated Virus Detection System Characterization of MS2 Bacteriophage written by and published by . This book was released on 2005 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study demonstrates the characterization of the sample matrix composition of the MS2 virus using ESI-MS and IVDS detection systems. The MS2 samples were grown and purified using various techniques, which showed ESI-MS samples responding differently from IVDS samples. The LC-MS of the specific biomarker of the MS2 bacteriophage from an infected Escherichia coli (E. coli) sample was characterized in the presence of E. coli proteins. The significant impact of the sample matrix was observed upon the identification of MS2 using a database search. Escherichia coli infected with MS2 showed a different score from other uninfected scores. Only purified MS2, using CsCl and analyzed by LS-MS, showed a positive match in the database search. However, the variation in the MS2 sample matrix had no effect on the characterization of MS2.
Book Synopsis Virus Detection: Limits and Strategies by : Charles Wick
Download or read book Virus Detection: Limits and Strategies written by Charles Wick and published by . This book was released on 1997 with total page 23 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid detection of virus particles is a vexing issue to the military and public health communities. The need for a rapid and trustworthy virus detector remains an urgent and continuing requirement for commanders requiring an assurance that they have early warning from these threats. This short report presents a historical summary, with references, of methods used to extract, purify, and concentrate viruses. Working with viruses is not an easy job. The requirement for detection was identified nearly 60 years ago and just now is seeing an approach with the evolution of the Integrated Virus Detector System (IVDS) device to undertake this exacting and difficult task. This work expounds a new methodology - IVDS. The IVDS utilizes a physical process to detect viruses. This is a departure from the traditional approaches that historically have utilized obscure chemical reactions, complicated reagents and other exacting and difficult procedures for detection. The IVDS is based in four physical stages which are collection, separation, purification, and detection. Development and risks are discussed. An IVDS development schedule and costs are given that could lead to an advance prototype in two years. The IVDS system promises a very sensitive, broad spectrum "generic" virus detector free from reagent based reactions, simple to operate, and providing a capability for continuous monitoring and recording of virus levels in the outdoor bioaerosol.
Book Synopsis Integrated Virus Detection System Characterization of MS2 and TBSV After Pulsed Lamp Exposure by :
Download or read book Integrated Virus Detection System Characterization of MS2 and TBSV After Pulsed Lamp Exposure written by and published by . This book was released on 2010 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to decontaminate microorganisms with a broad band pulsed light system has been demonstrated (ERDC-TR-456). This study will allow the effects of the pulsed lamp decontamination effects on MS2 bacteriophagc and tomato bushy stunt virus to be analyzed quickly without historical microbiological techniques. The Integrated Virus Detection System can analyze multiple samples quickly and allows rapid determination of pulsed lamp effects.
Book Synopsis Capillary Inlet Concentrator for the Integrated Virus Detection System (IVDS). by :
Download or read book Capillary Inlet Concentrator for the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2010 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: The standard method for concentration of virus samples with the Integrated Vims Detection System (IVDS) is to use a tangential flow filtration system to reduce the volume of the sample, while removing impurities such as salts and small cellular debris. This report investigated the IVDS' ability to concentrate the virus sample as well as remove impurities with a filtration attachment on the inlet side of the electrospray module. The filtration attachment or concentrator allowed the virus sample to be purified and concentrated and then analyzed with the IVDS without removing the sample from the IVDS.
Book Synopsis Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS). by :
Download or read book Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2005 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third report in a series of reports that investigates the survivability of the MS2 virus in harsh environments. The first two reports study the effects of pH on the virus. In this report, we study the effect of elevated temperatures on the virus. The stability of the virus was studied by counting the number of virus particles using the Integrated Virus Detection System (IVDS). Test results show that the virus particles are relatively stable for a limited time (up to 0.50 hr) at temperatures as high as 63 degrees C. Longer exposure or exposure to higher temperatures, results in quick breakdown of the virus particles.
Book Synopsis Conference on Hydrocarbon Emissions Control Policy by :
Download or read book Conference on Hydrocarbon Emissions Control Policy written by and published by . This book was released on 1976 with total page 47 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS). by :
Download or read book Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2006 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes testing of the survivability of MS2 virus under harsh conditions using the Integrated Virus Detection System (IVDS). In this study, the MS2 was subjected to a highly acidic and oxidative environment. Judging by the concentration of the virus particles, the virus survived 66 hr in 0.1 N HNO3 solution without any signs of diminution in the number of virus particles. This suggests that viruses might be able to survive such harsh environmental conditions.
Book Synopsis Virus Detection by : Charles H. Wick
Download or read book Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2023-05-03 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viruses do not behave as other microbes; their life cycles require infecting healthy cells, commandeering their cellular apparatus, replicating and then killing the host cell. Methods for virus detection and identification have been developed only in the past few decades. These recently developed methods include molecular, physical, and proteomic techniques. All these approaches (Electron Microscopy, Molecular, Direct Counting, and Mass Spectrometry Proteomics) to detection and identification are reviewed in this succinct volume. It is written in approachable language with enough detail for trained professionals to follow and want to recommend to others. Key Features Covers common detection methods Reviews the history of detection from antiquity to the present Documents the strengths and weaknesses of various detection methods Describes how to detect newly discovered viruses Recommends specific applications for clinical, hospital, environmental, and public health uses
Book Synopsis Molecular Methods for Virus Detection by : Danny L. Wiedbrauk
Download or read book Molecular Methods for Virus Detection written by Danny L. Wiedbrauk and published by Elsevier. This book was released on 1995-02-08 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables
