Immunoelectron Microscopy

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Immunoelectron Microscopy

Author : Steven D. Schwartzbach
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Immunoelectron Microscopy by : Steven D. Schwartzbach

Download or read book Immunoelectron Microscopy written by Steven D. Schwartzbach and published by Methods in Molecular Biology. This book was released on 2010-07-20 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships.

Microinjection and Transgenesis

Author : Angel Cid-Arregui
Publisher : Springer Science & Business Media
ISBN 13 : 3642803431
Total Pages : 619 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Microinjection and Transgenesis by : Angel Cid-Arregui

Download or read book Microinjection and Transgenesis written by Angel Cid-Arregui and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.

Mitochondria

Author : Dario Leister
Publisher : Springer Science & Business Media
ISBN 13 : 1588296679
Total Pages : 1195 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Mitochondria by : Dario Leister

Download or read book Mitochondria written by Dario Leister and published by Springer Science & Business Media. This book was released on 2007-06-12 with total page 1195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

Author : Alexander D. Hyatt
Publisher : CRC Press
ISBN 13 : 9780849367595
Total Pages : 476 pages
Book Rating : 4.3/5 (675 download)

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Book Synopsis Immuno-Gold Electron Microscopy in Virus Diagnosis and Research by : Alexander D. Hyatt

Download or read book Immuno-Gold Electron Microscopy in Virus Diagnosis and Research written by Alexander D. Hyatt and published by CRC Press. This book was released on 1992-11-10 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Electron Microscopy Methods and Protocols

Author : M. A. Nasser Hajibagheri
Publisher : Springer Science & Business Media
ISBN 13 : 1592592015
Total Pages : 292 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri

Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Immunoelectron Microscopy

Author : Kenneth H. Roux
Publisher :
ISBN 13 :
Total Pages : 95 pages
Book Rating : 4.:/5 (356 download)

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Book Synopsis Immunoelectron Microscopy by : Kenneth H. Roux

Download or read book Immunoelectron Microscopy written by Kenneth H. Roux and published by . This book was released on 1996 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Practical Electron Microscopy

Author : Elaine Evelyn Hunter
Publisher : Cambridge University Press
ISBN 13 : 9780521385398
Total Pages : 210 pages
Book Rating : 4.3/5 (853 download)

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Book Synopsis Practical Electron Microscopy by : Elaine Evelyn Hunter

Download or read book Practical Electron Microscopy written by Elaine Evelyn Hunter and published by Cambridge University Press. This book was released on 1993-09-24 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added--on immuno electron microscopy using colloidal gold and on useful specialized techniques.

Comparison of Electron Microscopy and Immunoelectron Microscopy with the Enzyme-linked Immunosorbent Assay for the Detection of Rotavirus and Antibody to the Virus

Download Comparison of Electron Microscopy and Immunoelectron Microscopy with the Enzyme-linked Immunosorbent Assay for the Detection of Rotavirus and Antibody to the Virus PDF Online Free

Author : Maria del Carmen Monedero
Publisher :
ISBN 13 :
Total Pages : 102 pages
Book Rating : 4.:/5 (612 download)

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Book Synopsis Comparison of Electron Microscopy and Immunoelectron Microscopy with the Enzyme-linked Immunosorbent Assay for the Detection of Rotavirus and Antibody to the Virus by : Maria del Carmen Monedero

Download or read book Comparison of Electron Microscopy and Immunoelectron Microscopy with the Enzyme-linked Immunosorbent Assay for the Detection of Rotavirus and Antibody to the Virus written by Maria del Carmen Monedero and published by . This book was released on 1979 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biomedical Electron Microscopy

Author : Arvid B. Maunsbach
Publisher : Elsevier
ISBN 13 : 0080528090
Total Pages : 569 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Biomedical Electron Microscopy by : Arvid B. Maunsbach

Download or read book Biomedical Electron Microscopy written by Arvid B. Maunsbach and published by Elsevier. This book was released on 1998-11-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs

Peroxisomes

Author : Michael Schrader
Publisher : Springer Nature
ISBN 13 : 1071630482
Total Pages : 507 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Peroxisomes by : Michael Schrader

Download or read book Peroxisomes written by Michael Schrader and published by Springer Nature. This book was released on 2023-03-23 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast, and this progress is reflected in the chapters included in this collection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peroxisomes: Methods and Protocols, Second Edition serves as an ideal guide for researchers working on peroxisome- and organelle-based research questions.

Electron Microscopic Immunocytochemistry

Author : Julia M. Polak
Publisher :
ISBN 13 :
Total Pages : 292 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Electron Microscopic Immunocytochemistry by : Julia M. Polak

Download or read book Electron Microscopic Immunocytochemistry written by Julia M. Polak and published by . This book was released on 1992 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.

An Immunoelectron Microscopic Study of Cell Surface Antigens Induced by Avian Tumor Viruses

Author : Edwin Robert Phillips
Publisher :
ISBN 13 :
Total Pages : 558 pages
Book Rating : 4.:/5 (89 download)

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Book Synopsis An Immunoelectron Microscopic Study of Cell Surface Antigens Induced by Avian Tumor Viruses by : Edwin Robert Phillips

Download or read book An Immunoelectron Microscopic Study of Cell Surface Antigens Induced by Avian Tumor Viruses written by Edwin Robert Phillips and published by . This book was released on 1974 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Electron Microscopy

Author : Pushpa Viswanathan
Publisher : MJP Publisher
ISBN 13 :
Total Pages : 570 pages
Book Rating : 4./5 ( download)

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Book Synopsis Electron Microscopy by : Pushpa Viswanathan

Download or read book Electron Microscopy written by Pushpa Viswanathan and published by MJP Publisher. This book was released on 2019-06-12 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of electron microscopes has opened up new vistas in the field of science. The ultrastructural morphological evidence offered by electron microscope to substantiate and support other findings is highly rewarding. This book gives a comprehensive overview of the principle and operations of the electron microscope. Numerous electron micrographs have been provided to acquaint the reader with the appearance of highly magnified features seen through the EM. This book would definitely create “a feel for this subject” particularly among those who want to use this technique for their research work.

Electron Microscopy of Plant Pathogens

Author : Kurt Mendgen
Publisher : Springer Science & Business Media
ISBN 13 : 3642758185
Total Pages : 342 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Electron Microscopy of Plant Pathogens by : Kurt Mendgen

Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.

Electron Microscopy in Diagnostic Virology

Author : Frances W. Doane
Publisher : CUP Archive
ISBN 13 : 9780521243117
Total Pages : 200 pages
Book Rating : 4.2/5 (431 download)

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Book Synopsis Electron Microscopy in Diagnostic Virology by : Frances W. Doane

Download or read book Electron Microscopy in Diagnostic Virology written by Frances W. Doane and published by CUP Archive. This book was released on 1987-01-30 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Autopsy Pathology: A Manual and Atlas

Author : Andrew J Connolly
Publisher : Elsevier Health Sciences
ISBN 13 : 0323287808
Total Pages : 403 pages
Book Rating : 4.3/5 (232 download)

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Book Synopsis Autopsy Pathology: A Manual and Atlas by : Andrew J Connolly

Download or read book Autopsy Pathology: A Manual and Atlas written by Andrew J Connolly and published by Elsevier Health Sciences. This book was released on 2015-09-23 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mainstay for pathology residents, Autopsy Pathology is designed with a uniquely combined manual and atlas format that presents today's most complete coverage of performing, interpreting, and reporting post-mortem examinations. This lasting and useful medical reference book offers a practical, step-by-step approach to discussing not only the basics of the specialty, but the performance of specialized autopsy procedures as well. Material is divided into two sections for ease of use: a manual covering specific autopsy procedures, biosafety, generation of autopsy reports, preparation of death certificates, and other essential subjects; and an atlas, organized by organ system, which captures the appearance of the complete spectrum of autopsy findings. Offers expanded coverage of microscopic anatomy. Includes a chapter on performing special dissection procedures that may not be covered during a typical residency. Examines important techniques, such as autopsy photography and radiology, microscopic examination, supplemental laboratory studies, and other investigative approaches. Addresses the latest legal, social, and ethical issues relating to autopsies, as well as quality improvement and assurance. Presents more than 600 full-color photographs depicting common gross and microscopic autopsy findings for every part of the body. Correlates pathologic findings with their clinical causes to enhance diagnostic accuracy. Improved images in the Atlas section provide greater visual understanding. Additional online features include dissection videos demonstrating autopsy techniques; downloadable, commonly used forms for autopsy reports; and calculators for weights and measures. Expert Consult eBook version included with purchase. This enhanced eBook experience offers access to all of the text, figures, images, videos, forms, calculators, and references from the book on a variety of devices.

Principles and Techniques of Electron Microscopy

Author : M. A. Hayat
Publisher : John Wiley & Sons
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Principles and Techniques of Electron Microscopy by : M. A. Hayat

Download or read book Principles and Techniques of Electron Microscopy written by M. A. Hayat and published by John Wiley & Sons. This book was released on 1970 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative mapping with the electron microscope; Photographic aspects of electron microscopy; Environmental devices in electron microscopy; Optical diffractometry; The analytical electron microscope, emma-4.