Author : Krystyna Sztefko
Publisher : Walter de Gruyter
ISBN 13 : 3110249480
Total Pages : 177 pages
Book Rating : 4.1/5 (12 download)
Book Synopsis Immunodiagnostics and Patient Safety by : Krystyna Sztefko
Download or read book Immunodiagnostics and Patient Safety written by Krystyna Sztefko and published by Walter de Gruyter. This book was released on 2011-05-26 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Today most of immunochemistry methods for the determination of proteins, peptides, drugs, and many small molecules are fully automated, with good precision, excellent sensitivity and short reaction time. However, inaccuracy due to poor standardization and the presence of interfering substances in biological samples is still a serious and life-threatening issue. Proper validation of methods and quality assurance have little effect on frequency of occurrence of false positive or false negative results, which, if unrecognized, may lead to patient's misdiagnosis, unnecessary treatment or even unnecessary surgery. Deep knowledge of basic principles of immunochemical methods (antigen-antibody reaction, standardization, matrix effect, limit of detection, cross-reactivity, etc.), sources of analyte-independent interferences (preanalitycal errors, the presence of binding proteins, the presence of autoantibodies) and analyte-dependent interferences (presence of heterophilic antibodies, high-dose effect) are very important to understand, detect, reduce and/or eliminate the interferences. This book helps to reduce false results and, at the same time, improve patient's care and patient's safety.