Identification And Expression Of G Protein Coupled Receptors

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Identification and Expression of G Protein-Coupled Receptors

Author : Kevin R. Lynch
Publisher : John Wiley & Sons
ISBN 13 : 9780471194934
Total Pages : 246 pages
Book Rating : 4.1/5 (949 download)

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Book Synopsis Identification and Expression of G Protein-Coupled Receptors by : Kevin R. Lynch

Download or read book Identification and Expression of G Protein-Coupled Receptors written by Kevin R. Lynch and published by John Wiley & Sons. This book was released on 1999-05-10 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen tremendous advances in the study of G protein-coupled receptors (GPCRs), including the molecular cloning and identification of more than 100 hundred GPCR genes. But while GPCRs serve as targets for more than 300 medicines in the modern pharmacopoeia, the shrinking pool of known ligands and the continuing discovery of orphan GPCR genes have underscored the need for new approaches to ligand identification. Identification and Expression of G Protein-Coupled Receptors addresses this new direction in GPCR biochemistry-offering a definitive laboratory bench manual that emphasizes expression over primary cloning strategies. In a series of expert contributions by well-known researchers, this book provides detailed protocols for various expression systems-from bacteria to mammalian cells-as well as straightforward opinions on the advantages and shortcomings of each approach. Topics covered include: * Homology screening and the polymerase chain reaction in the cloning of GPCR genes * Cloning of GPCRs using mammalian cell expression * GPCR informatics and the orphan problem * The use of Xenopus laevis oocytes for the study of GPCRs * Stable expression of GPCRs in mammalian cells * Heterologous expression in primary cell cultures * Expression of GPCR in Escherichia coli * Large scale expression and purification of GPCRs in mammalian cells * High-level expression of GPCRs in the Baculovirus/Sf9 cell expression system * Expression of GPCRs in Drosophila Schneider 2 cells * Methods for genetic analysis and ligand identification using heterologous GPCRs expressed in Saccharomyces cerevisiae Supplemented with numerous photographs and illustrations, Identification and Expression of G Protein-Coupled Receptors is important reading for biochemists, pharmacologists, neuroscientists, structural biologists, and anyone involved in GPCR-based research. It delivers a wealth of useful advice, practical tips, and invaluable insight into trends at the cutting-edge of current research.

Regulation of G Protein-coupled Receptor Function and Expression

Author : Jeffrey L. Benovic
Publisher :
ISBN 13 :
Total Pages : 330 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Regulation of G Protein-coupled Receptor Function and Expression by : Jeffrey L. Benovic

Download or read book Regulation of G Protein-coupled Receptor Function and Expression written by Jeffrey L. Benovic and published by . This book was released on 2000 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive text describes many techniques that are used to define the molecular mechanisms involved in receptor regulation. It covers a broad range of important concepts and methodologies in the study of G protein-coupled receptors.

The G Protein-Coupled Receptors Handbook

Author : Lakshmi A. Devi
Publisher : Springer Science & Business Media
ISBN 13 : 1592599192
Total Pages : 414 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis The G Protein-Coupled Receptors Handbook by : Lakshmi A. Devi

Download or read book The G Protein-Coupled Receptors Handbook written by Lakshmi A. Devi and published by Springer Science & Business Media. This book was released on 2008-03-01 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive survey of the many recent advances in the field of G protein-coupled receptors (GPCR). The authors describe the current knowledge of GPCR receptor structure and function, the different mechanisms involved in the regulation of GPCR function, and the role of pharmacological chaperones in GPCR folding and maturation. They also present new findings about how GPCR dimerization/oligomerization modifies the properties of individual receptors and show how recent developments are leading to significant advances in drug discovery, such as the detection of ligands for orphan GPCRs. Also discussed are the most recent developments that could lead to new drug discoveries: the role of GPCRs in mediating pain, the development of receptor-type selective drugs based on the structural plasticity of receptor activation, and the identification of natural ligands of orphan GPCRs (deorphanization) as possible drug targets.

Identification and Characterization of Th G Protein Coupled Receptors GPR100 (Homo Sapiens) and SALPR (Mus Musculus)

Author : Katrin Boels
Publisher : Tenea Verlag Ltd.
ISBN 13 : 3865041124
Total Pages : 66 pages
Book Rating : 4.8/5 (65 download)

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Book Synopsis Identification and Characterization of Th G Protein Coupled Receptors GPR100 (Homo Sapiens) and SALPR (Mus Musculus) by : Katrin Boels

Download or read book Identification and Characterization of Th G Protein Coupled Receptors GPR100 (Homo Sapiens) and SALPR (Mus Musculus) written by Katrin Boels and published by Tenea Verlag Ltd.. This book was released on 2005 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Neuromorphic Olfaction

Author : Krishna C. Persaud
Publisher : CRC Press
ISBN 13 : 1439871728
Total Pages : 237 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Neuromorphic Olfaction by : Krishna C. Persaud

Download or read book Neuromorphic Olfaction written by Krishna C. Persaud and published by CRC Press. This book was released on 2016-04-19 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many advances have been made in the last decade in the understanding of the computational principles underlying olfactory system functioning. Neuromorphic Olfaction is a collaboration among European researchers who, through NEUROCHEM (Fp7-Grant Agreement Number 216916)-a challenging and innovative European-funded project-introduce novel computing p

G Protein-Coupled Receptors in Drug Discovery

Author : Kenneth H. Lundstrom
Publisher : CRC Press
ISBN 13 : 1420028219
Total Pages : 376 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis G Protein-Coupled Receptors in Drug Discovery by : Kenneth H. Lundstrom

Download or read book G Protein-Coupled Receptors in Drug Discovery written by Kenneth H. Lundstrom and published by CRC Press. This book was released on 2005-07-11 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The broad range of G protein-coupled receptors (GPCRs) encompasses all areas of modern medicine and have an enormous impact on the process of drug development. Using disease-oriented methods to cover everything from screening to expression and crystallization, G Protein-Coupled Receptors in Drug Discovery describes the physiological roles of GPCRs

G Protein-Coupled Receptors - Part B

Author :
Publisher : Elsevier
ISBN 13 : 0323994350
Total Pages : 194 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis G Protein-Coupled Receptors - Part B by :

Download or read book G Protein-Coupled Receptors - Part B written by and published by Elsevier. This book was released on 2023-01-25 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: G Protein-Coupled Receptors, Part B, Volume 195 represents the largest class of cell H103 surface proteins that have intricate involvement in nearly every physiological process in our body. Approximately one third of the clinically prescribed medicines target GPCRs and the quest to improve the exiting GPCR-targeting therapeutics continues. Sections in this volume include Adhesion G protein-coupled receptors: Structure and functions, Molecular and cellular mechanisms underlying brain region-specific cannabinoid receptor modulation by estradiol across the rodent estrus cycle, Probing the orphan receptors: Tools and directions, Asymmetric activation of Class C GPCRs, Common and selective signal transduction mechanisms of GPCRs, and much more. Covers a broad range of topics related to GPCR activation and signaling Provides several receptor systems, with an emphasis on emerging paradigms Includes different processes and model systems, with a focus on GPCRs

G Protein-Coupled Receptors

Author : Xinfeng Zhao
Publisher : Springer Nature
ISBN 13 : 9819900786
Total Pages : 100 pages
Book Rating : 4.8/5 (199 download)

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Book Synopsis G Protein-Coupled Receptors by : Xinfeng Zhao

Download or read book G Protein-Coupled Receptors written by Xinfeng Zhao and published by Springer Nature. This book was released on 2023-02-20 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the progress made to functional immobilize G protein-coupled receptors (GPCRs) through site-specific or orientated recognition in both non-covalent and covalent manners. The last decade is the dawn of the “post-structural biology” era for G protein-coupled receptor research. As an emerging approach for state-of-the-art immobilization, this book discusses efforts to explore the elegance of naturally-occurring biochemical reactions by using their high specificity and robust reactivity in the complex system, such as site-specific conjugation by covalent recognition between enzymes and their substrates. With the perspective of protein-drug interactions, this book also reviews the applications of protein immobilization, with an emphasis on G protein-coupled receptors, in drug discovery and protein-ligand interaction analysis. In addition, the merits, opportunities and disadvantages are analyzed for different immobilization methods, and a perspective for future directions is presented. Given its scope, this book appeals to a broad readership, particularly researchers engaged in the field of analytical chemistry, bioconjugate chemistry, and chemical biology, and other related field, as well as teachers of relevant majors in colleges and universities.

G Protein-Coupled Receptors

Author : Gabriel Berstein
Publisher : CRC Press
ISBN 13 : 9781420048513
Total Pages : 440 pages
Book Rating : 4.0/5 (485 download)

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Book Synopsis G Protein-Coupled Receptors by : Gabriel Berstein

Download or read book G Protein-Coupled Receptors written by Gabriel Berstein and published by CRC Press. This book was released on 2019-04-24 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering recently developed methods in membrane-bound receptors, this book emphasizes receptor structure and function, knowledge of which is essential to the study of signal transduction. G Protein-Coupled Receptors has culled contributors from domestic and international sources, providing a broad base of knowledge. Some topics covered are the r

G Protein-Coupled Receptors

Author : Jesus Giraldo
Publisher : Royal Society of Chemistry
ISBN 13 : 1849733449
Total Pages : 549 pages
Book Rating : 4.8/5 (497 download)

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Book Synopsis G Protein-Coupled Receptors by : Jesus Giraldo

Download or read book G Protein-Coupled Receptors written by Jesus Giraldo and published by Royal Society of Chemistry. This book was released on 2011-08-16 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: G protein-coupled receptors (GPCRs) are the largest family of cell-surface receptors, with more than 800 members identified thus far in the human genome. They regulate the function of most cells in the body, and represent approximately 3% of the genes in the human genome. These receptors respond to a wide variety of structurally diverse ligands, ranging from small molecules, such as biogenic amines, nucleotides and ions, to lipids, peptides, proteins, and even light. Ligands (agonists and antagonists) acting on GPCRs are important in the treatment of numerous diseases, including cardiovascular and mental disorders, retinal degeneration, cancer, and AIDS. It is estimated that these receptors represent about one third of the actual identified targets of clinically used drugs. The determination of rhodopsin crystal structure and, more recently, of opsin, 1 and 2 adrenergic and A2A adenosine receptors provides both academia and industry with extremely valuable data for a better understanding of the molecular determinants of receptor function and a more reliable rationale for drug design. GPCR structure and function constitutes a hot topic. The book, which lies between the fields of chemical biology, molecular pharmacology and medicinal chemistry, is divided into three parts. The first part considers what receptor structures tell us about the mechanism of receptor activation. Part II focuses on receptor function. It discusses what the data from biophysical and mutational studies, and the analysis of the interactions of the receptor with ligands and regulator proteins, tell us about the process of signal transduction. The final part, on modelling and simulation, details new insights on the link between structure and mechanism and their implications in drug design.

G Protein-Coupled Receptor Screening Assays

Author : Sofia Aires M. Martins
Publisher : Humana
ISBN 13 : 9781071612231
Total Pages : 327 pages
Book Rating : 4.6/5 (122 download)

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Book Synopsis G Protein-Coupled Receptor Screening Assays by : Sofia Aires M. Martins

Download or read book G Protein-Coupled Receptor Screening Assays written by Sofia Aires M. Martins and published by Humana. This book was released on 2022-06-19 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.

Identifying Novel G Protein-coupled Receptor Targets in Pulmonary Hypertension

Author : Jean Rose Iyinikkel
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (111 download)

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Book Synopsis Identifying Novel G Protein-coupled Receptor Targets in Pulmonary Hypertension by : Jean Rose Iyinikkel

Download or read book Identifying Novel G Protein-coupled Receptor Targets in Pulmonary Hypertension written by Jean Rose Iyinikkel and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interestingly the CRISPR-GPR75 KO mouse is viable and shows no obvious phenotype. We hypothesise that exposing these CRISPR-GPR75 KO mice to hypoxia will protect them from developing PAH. Taken together our data show the utility of profiling GPCRs in disease and highlight GPCR expression and function could contribute to the sex bias in PAH. We have generated a number of tools that will aide in fully uncovering the pharmacological and physiological role of GPR75. Overall we have validated GPR75 as a novel therapeutic target in PAH; inhibitors of GPR75 would be expected to blunt or reverse remodelling of the pulmonary artery associated with the disease.

G Protein-Coupled Receptors

Author : Tatsuya Haga
Publisher : CRC Press
ISBN 13 : 1420038273
Total Pages : 336 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis G Protein-Coupled Receptors by : Tatsuya Haga

Download or read book G Protein-Coupled Receptors written by Tatsuya Haga and published by CRC Press. This book was released on 2005-08-04 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a broad base of knowledge of G-protein-coupled receptors. Useful at both the university and industrial levels, this book is of particular interest to those who are developing therapeutic approaches to diseases using drugs that influence receptor activation.

Adhesion G Protein-coupled Receptors

Author : Tobias Langenhan
Publisher : Springer
ISBN 13 : 3319415239
Total Pages : 409 pages
Book Rating : 4.3/5 (194 download)

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Book Synopsis Adhesion G Protein-coupled Receptors by : Tobias Langenhan

Download or read book Adhesion G Protein-coupled Receptors written by Tobias Langenhan and published by Springer. This book was released on 2016-11-09 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: Latest research on Adhesion GPCRs has unearthed surprising revelations about the events that govern the signal transduction of these receptor molecules and the cellular and organ requirements for these signals. Unexpected and unprecedented findings suggest that Adhesion GPCRs constitute a group of receptors that sense mechanical stimuli and transcode them into metabotropic signals through the action of a novel activation paradigm. Interdisciplinary efforts transcending many areas of biomedical research including pharmacology, physiology, genetics, cell biology, structural biology, biochemistry and bioinformatics were necessary to unveil these fundamental properties. The scientific leaders in the field that carried this research effort have teamed up here to provide a comprehensive overview of our current understanding, how Adhesion GPCRs signal and how these receptors shape organ structure and function.

GPCRs: From Deorphanization to Lead Structure Identification

Author : H. Bourne
Publisher : Springer Science & Business Media
ISBN 13 : 3540489819
Total Pages : 275 pages
Book Rating : 4.5/5 (44 download)

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Book Synopsis GPCRs: From Deorphanization to Lead Structure Identification by : H. Bourne

Download or read book GPCRs: From Deorphanization to Lead Structure Identification written by H. Bourne and published by Springer Science & Business Media. This book was released on 2007-07-03 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an insight into the approaches taken by industry and academia to address GPCRs and depict how mature this target class-oriented research has become in the last decade. Coverage also reflects the actual trends in the fast-emerging field of GPCR research in academia and industry. It is based on the international workshop GPCRs: From Deorphanisation to Lead Structure Identification, held in Berlin in May 2006.

Receptor-Receptor Interactions

Author : Kjell Fuxe
Publisher : Springer Science & Business Media
ISBN 13 : 1468454153
Total Pages : 577 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Receptor-Receptor Interactions by : Kjell Fuxe

Download or read book Receptor-Receptor Interactions written by Kjell Fuxe and published by Springer Science & Business Media. This book was released on 2013-03-13 with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt:

G Protein Coupled Receptors

Author :
Publisher : Academic Press
ISBN 13 : 0123918731
Total Pages : 489 pages
Book Rating : 4.1/5 (239 download)

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Book Synopsis G Protein Coupled Receptors by :

Download or read book G Protein Coupled Receptors written by and published by Academic Press. This book was released on 2013-01-24 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers G protein coupled receptors - Contains chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high-throughput assays for identifying pharmacoperone drugs