Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
High Speed Atomic Force Microscopy
Download High Speed Atomic Force Microscopy full books in PDF, epub, and Kindle. Read online High Speed Atomic Force Microscopy ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Noncontact Atomic Force Microscopy by : S. Morita
Download or read book Noncontact Atomic Force Microscopy written by S. Morita and published by Springer Science & Business Media. This book was released on 2002-07-24 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since 1995, the noncontact atomic force microscope (NC-AFM) has achieved remarkable progress. Based on nanomechanical methods, the NC-AFM detects the weak attractive force between the tip of a cantilever and a sample surface. This method has the following characteristics: it has true atomic resolution; it can measure atomic force interactions, i.e. it can be used in so-called atomic force spectroscopy (AFS); it can also be used to study insulators; and it can measure mechanical responses such as elastic deformation. This is the first book that deals with all of the emerging NC-AFM issues.
Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró
Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-05-14 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM
Book Synopsis Encyclopedia of Biophysics by : Gordon Roberts
Download or read book Encyclopedia of Biophysics written by Gordon Roberts and published by Springer. This book was released on 2012-10-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia of Biophysics is envisioned both as an easily accessible source of information and as an introductory guide to the scientific literature. It includes entries describing both Techniques and Systems. In the Techniques entries, each of the wide range of methods which fall under the heading of Biophysics are explained in detail, together with the value and the limitations of the information each provides. Techniques covered range from diffraction (X-ray, electron and neutron) through a wide range of spectroscopic methods (X-ray, optical, EPR, NMR) to imaging (from electron microscopy to live cell imaging and MRI), as well as computational and simulation approaches. In the Systems entries, biophysical approaches to specific biological systems or problems – from protein and nucleic acid structure to membranes, ion channels and receptors – are described. These sections, which place emphasis on the integration of the different techniques, therefore provide an inroad into Biophysics from a biological more than from a technique-oriented physical/chemical perspective. Thus the Encyclopedia is intended to provide a resource both for biophysicists interested in methods beyond those used in their immediate sub-discipline and for those readers who are approaching biophysics from either a physical or biological background.
Book Synopsis High-Speed Atomic Force Microscopy in Biology by : Toshio Ando
Download or read book High-Speed Atomic Force Microscopy in Biology written by Toshio Ando and published by Springer Nature. This book was released on 2022-03-23 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Numerous examples of HS-AFM studies on proteins demonstrate how to apply this new form of microscopy to specific biological problems. Several precautions for successful imaging and the preparation of cantilever tips and substrate surfaces will greatly benefit first-time users of HS-AFM. In turn, the instrumentation techniques detailed in Chapter 4 can be skipped, but will be useful for engineers and scientists who want to develop the next generation of high-speed scanning probe microscopes for biology. The book is intended to facilitate the first-time use of this new technique, and to inspire students and researchers to tackle their own specific biological problems by directly observing dynamic events occurring in the nanoscopic world. Microscopy in biology has recently entered a new era with the advent of high-speed atomic force microscopy (HS-AFM). Unlike optical microscopy, electron microscopy, and conventional slow AFM, it allows us to directly observe biological molecules in physiological environments. Molecular “movies” created using HS-AFM can directly reveal how molecules behave and operate, without the need for subsequent complex analyses and roundabout interpretations. It also allows us to directly monitor morphological change in live cells, and dynamic molecular events occurring on the surfaces of living bacteria and intracellular organelles. As HS-AFM instruments were recently commercialized, in the near future HS-AFM is expected to become a common tool in biology, and will enhance and accelerate our understanding of biological phenomena.
Book Synopsis Biophysical Analysis of Membrane Proteins by : Eva Pebay-Peyroula
Download or read book Biophysical Analysis of Membrane Proteins written by Eva Pebay-Peyroula and published by Wiley-Blackwell. This book was released on 2008 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.
Book Synopsis Atomic Force Microscopy by : Nuno C. Santos
Download or read book Atomic Force Microscopy written by Nuno C. Santos and published by Humana Press. This book was released on 2018-10-30 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.
Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró
Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-08-01 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM
Book Synopsis Atomic Force Microscopy in Nanobiology by : Kunio Takeyasu
Download or read book Atomic Force Microscopy in Nanobiology written by Kunio Takeyasu and published by CRC Press. This book was released on 2016-04-19 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in atomic force microscopy (AFM) have been accomplished through various technical and instrumental innovations, including high-resolution and recognition imaging technology under physiological conditions, fast-scanning AFM, and general methods for cantilever modification and force measurement. All these techniques are now highly
Book Synopsis Single Molecule Dynamics in Life Science by : Toshio Yanagida
Download or read book Single Molecule Dynamics in Life Science written by Toshio Yanagida and published by John Wiley & Sons. This book was released on 2008-11-24 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
Book Synopsis Atomic Force Microscopy by : Peter Eaton
Download or read book Atomic Force Microscopy written by Peter Eaton and published by Oxford University Press. This book was released on 2010-03-25 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopes are very important tools for the advancement of science and technology. This book provides an introduction to the microscopes so that scientists and engineers can learn both how to use them, and what they can do.
Book Synopsis Control System Design for High-Speed Atomic Force Microscopy by : Nastaran Nikooienejad
Download or read book Control System Design for High-Speed Atomic Force Microscopy written by Nastaran Nikooienejad and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Video-rate atomic force microscopy is in high demand to visualize dynamic processes in realtime, whereas the functionality of a commercial atomic force microscope (AFM) is restricted to low-speed scans. In the past decades, extensive research efforts have aimed to reinforce the AFM structure toward high-speed atomic force microscopy. However, video-rate imaging of a relatively large scan area is still challenging due to the highly resonant nature of AFM and its conventional method of scanning. The AFM control system also needs to be significantly improved to harness the full potential of AFM mechanical structure at video rate and provide adequate robustness during scan. Therefore, besides the AFM configuration, scanning methods and control techniques contribute significantly to achieving the ultimate goal of sequential AFM imaging at video rate. This dissertation focuses on novel scanning methods and control design methodologies that facilitate sequential atomic force microscopy and improve positioning accuracy at high speed. First, we leverage a technique to smoothen the sequential cycloid trajectory and mitigate the sudden back and forth motions of a positioner in capturing successive frames of a scan. The resulting trajectory reduces the residual tracking error and enhances the AFM image quality. We also propose a systematic design methodology for a novel repetitive non-raster scan trajectory based on the rosette pattern. This pattern, generated by pure harmonic waveforms, can address the conventional issues with sequential non-raster imaging by enabling a smooth and continuous scan. We provide a thorvii ough mathematical analysis of the rosette pattern and a step-by-step design procedure for rosette scanning. We proceed by proposing high-precision model-based control design approaches for sequential AFM imaging using a microelectromechanical system (MEMS) nanopositioner. To precisely follow the reference setpoints in sequential cycloid and rosette scans, we design a tracking controller based on the internal model principle. The internal-model-based controller (IMBC) is intuitive and well-suited for tracking non-raster scan patterns. The controller incorporates the fundamental reference frequencies and their corresponding higher harmonics to reduce the deterministic error originated from uncompensated nonlinearities in the system. However, the resulting controller is of high order, and requires a priori knowledge of the dominant harmonics in the experimental tracking error. To resolve this, we develop a novel control scheme involving an internal-model-based control in feedback and an iterative learning control in feedforward. The internal-model-based controller only includes fundamental frequencies of the references while the iterative learning controller rejects the induced higher harmonics by learning from past experiences. The proposed control scheme is employed for tracking the rosette pattern at various scan rates. We investigate the performance of the proposed scanning methods and control techniques in closed-loop experiments. Finally, a series of high-quality images are obtained at high speed using a MEMS nanopositioner and a commercial AFM. A limiting factor toward high-speed atomic force microscopy is the lightly damped nature of the scanners. To increase the imaging bandwidth and scan speed, vibration control techniques have been practiced. Among them, fixed-structure strictly negative imaginary (SNI) controllers ensure robust stability of closed-loop system when the scanner incorporates collocated actuator and sensor pairs. In the third section of this dissertation, we present a convex synthesis of SNI controllers for a class of multi-input multi-output (MIMO) plants satisfying the negative imaginary property. The design procedure is based on the frequency response data of the plant, and the control objective is to minimize the distance between a desired and actual closed-loop frequency response. The controllers are experimentally implemented to augment damping to the fundamental resonant mode of a MEMS nanopositioner in a two-input two-output configuration.
Book Synopsis Atomic Force Microscopy in Molecular and Cell Biology by : Jiye Cai
Download or read book Atomic Force Microscopy in Molecular and Cell Biology written by Jiye Cai and published by Springer. This book was released on 2018-11-03 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book addresses new achievements in AFM instruments – e.g. higher speed and higher resolution – and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc.
Download or read book Myosins written by Lynne M. Coluccio and published by Springer Science & Business Media. This book was released on 2007-11-15 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly authoritative volume highlights the remarkable superfamily of molecular motors called myosins, which are involved in such diverse cellular functions as muscle contraction, intracellular transport, cell migration and cell division. In a timely compilation of chapters written by leading research groups that have made key discoveries in the field, the current understanding of the molecular mechanisms and biological functions of these intriguing proteins is explored.
Book Synopsis Nanoscale Imaging by : Yuri L. Lyubchenko
Download or read book Nanoscale Imaging written by Yuri L. Lyubchenko and published by Humana Press. This book was released on 2018-06-29 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.
Book Synopsis Life at the Nanoscale by : Yves Dufrene
Download or read book Life at the Nanoscale written by Yves Dufrene and published by CRC Press. This book was released on 2019-03-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.
Book Synopsis High Speed Atomic Force Microscopy by : Younkoo Jeong
Download or read book High Speed Atomic Force Microscopy written by Younkoo Jeong and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Since its introduction more than twenty years ago, Atomic Force Microscopy (AFM) has extended its application areas from material science to biology or biophysics, based on its capability to image/manipulate objects in various environments with sub-nanometer spatial resolution in three dimensions. Among the two most commonly used modes, the dynamic (tapping) mode has a great advantage over contact mode when imaging soft materials, minimizing potentially destructive shear and adhesive forces on the sample. The amplitude modulation is the most commonly used control method in the dynamic mode of AFM, in which the oscillation amplitude of the cantilever is regulated. However, in typical implementations, due to tapping dynamics of the AFM cantilevers, the transient response of the cantilever induced by changes of the tip-sample interaction force leads to greater variations in tip-sample interaction via feedback, causing excessive tapping forces and/or possible loss of tapping during the scanning, and thus greater sample distortions and imaging errors. In addition, the low bandwidth of the actuators in conventional AFMs, such as the z-positioner and the raster scanner limits the scanning speed. Therefore, while dynamic mode AFM has many potential applications, the inability to achieve direct and precise control of the tip-sample interaction forces and the low bandwidth of actuators for the tip-sample separation control and the raster scanning have been key barriers which imaging rate and inhibit innovation leading to new applications. In this research, the design, actuation and control of a new generation AFM probing system which enables high-speed and high-resolution imaging of samples are investigated. In order to achieve direct tip-sample interaction control during the scanning, a novel dynamic sensing and control method are implemented, in which the tip-sample interaction force of each tapping cycle is estimated and subsequently controlled for dynamic force microscopy. By employing collocated magnetic actuation of the AFM cantilever and dual-actuator tip-motion control scheme, the high bandwidth tip-motion control, whose bandwidth is comparable to that of the cantilever, the dynamics over-damped, and the motion range comparable to that of conventional z-scanner is achieved. For the high bandwidth raster scanning as well as high bandwidth tip-sample separation control, active multi-axis probing system is implemented, in which multi-axis magnetic actuators along with a multi-axis probe with one magnetic moment, specially designed and fabricated for the multi-axis actuation, achieves high bandwidth multi-axis tip-motion control along the Z axis and the X axis. In order to achieve the high resolution imaging, a low noise laser measurement system is implemented and integrated to a commercial AFM (MFP3D, Asylum research). For the implementation of the direct tip-sample interaction control and high bandwidth active multi-axis probing system, high speed programmable digital controller is developed using field programmable gate array (FPGA) whose closed loop update rate is two orders of magnitude higher than commercially available ones. The results of scanning a standard grating whose pitch is 100nm and a biological grating (repeating protein structure on purple membranes) whose lateral pitch is about 6.2 nm using the high speed AFM are presented and discussed.
Book Synopsis Atomic Force Microscopy for Nanoscale Biophysics by : Mi Li
Download or read book Atomic Force Microscopy for Nanoscale Biophysics written by Mi Li and published by Elsevier. This book was released on 2023-02-15 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells. The book discusses the methodology of AFM-based biomedical detection, diverse biological systems, and the combination of AFM with other complementary techniques. These state-of-the-art chapters empower researchers to address biological issues through the application of atomic force microscopy. Atomic force microscopy (AFM) is a unique, multifunctional tool for investigating the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution. Summarizes the recent progress of atomic force microscopy in biomedical applications Presents the methods and skills of applying atomic force microscopy Aids researchers in investigating the nanoscale biophysics of diverse biological systems
