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High Resolution X Ray Diffraction Microscopy Of Specifically Labeled Yeast Cells Cxidb Id 4
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Book Synopsis High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 4). by :
Download or read book High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 4). written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first of five exposures of the same sample at different tilts. This one is at +0 degrees tilt. Check CXI IDs 4 to 8 for the complete set.
Book Synopsis High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 8). by :
Download or read book High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 8). written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the fifth of five exposures of the same sample at different tilts. This one is at -30 degrees tilt. Check CXI IDs 4 to 8 for the complete set.
Book Synopsis High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 5). by :
Download or read book High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 5). written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of five exposures of the same sample at different tilts. This one is at +15 degrees tilt. Check CXI IDs 4 to 8 for the complete set.
Book Synopsis High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 7). by :
Download or read book High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 7). written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the fourth of five exposures of the same sample at different tilts. This one is at -15 degrees tilt. Check CXI IDs 4 to 8 for the complete set.
Book Synopsis High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 6). by :
Download or read book High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells (CXIDB ID 6). written by and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third of five exposures of the same sample at different tilts. This one is at +30 degrees tilt. Check CXI IDs 4 to 8 for the complete set.
Book Synopsis High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells by :
Download or read book High-resolution X-ray Diffraction Microscopy of Specifically Labeled Yeast Cells written by and published by . This book was released on 2010 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific gold labeling at different depths within cells via through-focus propagation of the reconstructed wavefield. The lectin concanavalin A conjugated to colloidal gold particles was used to label the [alpha]-mannan sugar in the cell wall of the yeast Saccharomyces cerevisiae. Cells were plunge-frozen in liquid ethane and freeze-dried, after which they were imaged whole using x-ray diffraction microscopy at 750 eV photon energy.
Book Synopsis Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell by :
Download or read book Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell written by and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.
Book Synopsis Reconstruction of a Yeast Cell from X-ray Diffraction Data by :
Download or read book Reconstruction of a Yeast Cell from X-ray Diffraction Data written by and published by . This book was released on 2006 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details are provided of the algorithm used for the reconstruction of yeast cell images in the recent demonstration of diffraction microscopy by Shapiro, Thibault, Beetz, Elser, Howells, Jacobsen, Kirz, Lima, Miao, Nieman & Sayre. Two refinements of the iterative constraint-based scheme are developed to address the current experimental realities of this imaging technique, which include missing central data and noise. A constrained power operator is defined whose eigenmodes allow the identification of a small number of degrees of freedom in the reconstruction that are negligibly constrained as a result of the missing data. To achieve reproducibility in the algorithm's output, a special intervention is required for these modes. Weak incompatibility of the constraints caused by noise in both direct and Fourier space leads to residual phase fluctuations. This problem is addressed by supplementing the algorithm with an averaging method. The effect of averaging may be interpreted in terms of an effective modulation transfer function, as used in optics, to quantify the resolution. The reconstruction details are prefaced with simulations of wave propagation through a model yeast cell. These show that the yeast cell is a strong-phase-contrast object for the conditions in the experiment.
