High-Performance Liquid Chromatography of Proteins and Peptides

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Publisher : Elsevier
ISBN 13 : 032315770X
Total Pages : 284 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis High-Performance Liquid Chromatography of Proteins and Peptides by : Milton Hearn

Download or read book High-Performance Liquid Chromatography of Proteins and Peptides written by Milton Hearn and published by Elsevier. This book was released on 2012-12-02 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.

High-Performance Liquid Chromatography of Peptides and Proteins

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Author :
Publisher : CRC Press
ISBN 13 : 1351440756
Total Pages : 960 pages
Book Rating : 4.3/5 (514 download)

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Book Synopsis High-Performance Liquid Chromatography of Peptides and Proteins by : Colin T. Mant

Download or read book High-Performance Liquid Chromatography of Peptides and Proteins written by Colin T. Mant and published by CRC Press. This book was released on 2017-11-22 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

HPLC of Peptides and Proteins

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1592597424
Total Pages : 411 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis HPLC of Peptides and Proteins by : Marie-Isabel Aguilar

Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

High-performance Liquid Chromatography of Proteins and Peptides

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (664 download)

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Book Synopsis High-performance Liquid Chromatography of Proteins and Peptides by :

Download or read book High-performance Liquid Chromatography of Proteins and Peptides written by and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

High Performance Liquid Chromatography of Proteins and Peptides

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Publisher :
ISBN 13 : 9780471828426
Total Pages : pages
Book Rating : 4.8/5 (284 download)

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Book Synopsis High Performance Liquid Chromatography of Proteins and Peptides by : Regnier

Download or read book High Performance Liquid Chromatography of Proteins and Peptides written by Regnier and published by . This book was released on 1997-05-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Gel Permeation and Ion-Exchange Chromatography of Proteins and Peptides

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Author :
Publisher : CRC Press
ISBN 13 : 1000083179
Total Pages : 258 pages
Book Rating : 4.0/5 ( download)

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Book Synopsis Gel Permeation and Ion-Exchange Chromatography of Proteins and Peptides by : Kato

Download or read book Gel Permeation and Ion-Exchange Chromatography of Proteins and Peptides written by Kato and published by CRC Press. This book was released on 2020-03-24 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a collection of critical reviews of the use of high-performance liquid chromatography in a very specialized area of research. It describes in detail modern methodology to separate nucleic acids, enzymes and a wide variety of biologically active proteins such as renin.

High Performance Liquid Chromatography of Proteins and Peptides

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Publisher :
ISBN 13 :
Total Pages : 267 pages
Book Rating : 4.:/5 (867 download)

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Book Synopsis High Performance Liquid Chromatography of Proteins and Peptides by : Milton T. W. Hearn

Download or read book High Performance Liquid Chromatography of Proteins and Peptides written by Milton T. W. Hearn and published by . This book was released on 1983 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt:

HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES & PROTEINS

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Publisher : Springer
ISBN 13 :
Total Pages : 520 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES & PROTEINS by : William S. Hancock

Download or read book HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES & PROTEINS written by William S. Hancock and published by Springer. This book was released on 1984-07-02 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: V.1 - HPLC Instrumentation; Mobile phases; Detection nethods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives; Separation of peptides; Analysis of biologically active peptides; Use of HPLC in protein sequencing; Protein separations; Examples of protein separations by reversed-phase HPLC; v.2 - Separation of peptides; Analysis of biologically active peptides; Protein separations; Examples of protein separations by reversed-phase HPLC.

Handbook HPLC for Separation Amino Acids Peptides and Proteins

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Publisher : CRC-Press
ISBN 13 : 9780849335112
Total Pages : 544 pages
Book Rating : 4.3/5 (351 download)

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Book Synopsis Handbook HPLC for Separation Amino Acids Peptides and Proteins by : William S. Hancock

Download or read book Handbook HPLC for Separation Amino Acids Peptides and Proteins written by William S. Hancock and published by CRC-Press. This book was released on 1984-08-14 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.

High-performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (437 download)

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Book Synopsis High-performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : Erich Heftmann

Download or read book High-performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by Erich Heftmann and published by . This book was released on 1986 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : 665 pages
Book Rating : 4.:/5 (635 download)

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Book Synopsis International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

Download or read book International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides and published by . This book was released on 1983 with total page 665 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Seventh International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (159 download)

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Book Synopsis Seventh International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides (7, 1987, Washington, DC)

Download or read book Seventh International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides (7, 1987, Washington, DC) and published by . This book was released on 1988 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : 448 pages
Book Rating : 4.:/5 (635 download)

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Book Synopsis International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

Download or read book International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides and published by . This book was released on 1990 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : 445 pages
Book Rating : 4.:/5 (635 download)

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Book Synopsis International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

Download or read book International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides and published by . This book was released on 1985 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Third International Symposium of High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (313 download)

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Book Synopsis Third International Symposium of High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

Download or read book Third International Symposium of High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Author :
Publisher :
ISBN 13 :
Total Pages : 274 pages
Book Rating : 4.:/5 (635 download)

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Book Synopsis International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

Download or read book International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High-Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides and published by . This book was released on 1992 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fourth International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (313 download)

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Book Synopsis Fourth International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides by : International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides (4, 1984, Baltimore, Md.)

Download or read book Fourth International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides written by International Symposium on High Performance Liquid Chromatography of Proteins, Peptides and Polynucleotides (4, 1984, Baltimore, Md.) and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: