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Glycopeptide Detection Selection And Characerization Through A Liquid Chromatography Mass Spectrometry Differential Ion Mobility Platform
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Book Synopsis Glycopeptide Detection, Selection, and Characerization Through a Liquid Chromatography-mass Spectrometry-differential Ion Mobility Platform by : Daniel Graham Delafield
Download or read book Glycopeptide Detection, Selection, and Characerization Through a Liquid Chromatography-mass Spectrometry-differential Ion Mobility Platform written by Daniel Graham Delafield and published by . This book was released on 2018 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Application of Liquid Chromatography-mass Spectrometry and Differential Mobility Spectrometry-mass Spectrometry for Detection and Quantitation of Biomarkers in Complex Biological Matrices by : Zhidan Chen
Download or read book Application of Liquid Chromatography-mass Spectrometry and Differential Mobility Spectrometry-mass Spectrometry for Detection and Quantitation of Biomarkers in Complex Biological Matrices written by Zhidan Chen and published by . This book was released on 2017 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: The projects and discussions in this dissertation are about utilizing modern separation technologies coupled with mass spectrometry for detection and quantitation of various biomarkers in complex biological matrices. Based on the differences of separation phases, the separation technologies in this dissertation will be divided into two directions: Differential Mobility Spectrometry (DMS, one of the ion mobility technologies) in gas phase and Liquid Chromatography (LC) in liquid phase. The projects and applications of each technology will be discussed separately. However, despite the differences of DMS and LC, they can serve as a complementary method for each other. Together, the combination of Ion Mobility technology with Liquid Chromatography technology nowadays have been widely used in both modern life science industry and academia, and will continue being utilized as the workhorse technologies in the near future. This dissertation is mainly divided into three parts: Part I is about the utility of Differential Mobility Spectrometry-Mass Spectrometry (DMS-MS) in radiation biodosimetry. Part II is about the utility of Liquid Chromatography-Mass Spectrometry (LC-MS) in DNA adducts analysis. There are three chapters in each part. Chapter 1-3 are for part I, Chapter 4-6 are for part II. Part III is the conclusions and future directions for this dissertation. Chapter 1 is a relatively short but well-covered introduction about the background of the first project, DMS-MS with its application in biodosimetry. Four major types of ion mobility technologies including drift tube, travelling wave, trapped ion mobility and DMS/FAIMS are summarized and compared in this chapter, with DMS being the focus. Then the history of the development of DMS technology and its development and applications in Vouros lab are introduced, followed with the background of its application in biodosimetry as a rapid and high-throughput screening technology. In chapter 2, a preliminary study of utilizing DMS-MS for rapid and high-throughput detection and quantitation of radiation biomarkers in nonhuman primate urine is shown. In this study, we explored the capability of DMS-MS as an alternative platform for rapid assessment of radiation exposure since the traditional LC-MS platform is too slow due to the nature of chromatography and thus cannot be used for high-throughput analysis purposes. Two radiation biomarkers hypoxanthine and trimethyllysine were chosen for the analysis. The calibration curves and validation tests for each compound were built within 2 hours which is about 10-20 times faster than LC-MS. The level of hypoxanthine in control and 10 Gy radiated group of nonhuman primate urine was determined by this DMS-MS platform and showed a dose response similar to the one obtained by LC-MS but in a much faster manner. This preliminary study has shown that our DMS-MS platform is capable of doing in-field and in-clinic rapid screening of radiation exposure. In chapter 3, as a continuing study of chapter 2, we applied DMS-MS method for simultaneously monitoring multiple radiation biomarkers rather than a single biomarker as shown in the preliminary study in order to achieve better and more confident assessment of radiation exposure. The calibration curves and dose response experiments for all the biomarkers in this study were conducted and showed a much faster throughput than LC-MS as also shown in chapter 2. In chapter 4, the background of DNA adducts and its relationship with cancer, followed by an introduction of using LC-MS as the tool for analysis of DNA adducts, especially the DNA adduct dG-C8-4-ABP in bladder cancer, are introduced. Different mass analyzers and scanning modes are also included in this chapter to give a more detailed information about how to conduct DNA adducts screening. In chapter 5, a relatively detailed review of recent technical and biological development in the analysis of the DNA adduct dG-C8-4-ABP is shown, which allows for a more comprehensive understanding of the whole process of the analysis of DNA adducts, the carcinogenesis of 4-ABP, different methods that can be used for detection and quantitation of dG-C8-4-ABP or other DNA adducts and their comparisons. This chapter is only focused on this single DNA adduct since it is one of the most studied DNA adducts in our laboratory, but the information applies to any other kind of adducts. The different methods of analyzing dG-C8-4-ABP will be covered and discussed thoroughly, from the traditional ℗đ℗øP-postlabelling to the most recent nanoLC-MS and UPLC-MS. In the end, there is a very short introduction about the gender disparity in bladder cancer which is potentially related with hepatic Sulfotransferase (Sult) enzymes. This is a connection linked to chapter 6 which presented a detailed search for the key Sult enzyme/enzymes that are responsible for gender disparity in bladder cancer, and its long-term implications. In chapter 6, as mentioned above, a thorough investigation about the role of hepatic Sult enzymes in the formation of dG-C8-4-ABP in both liver and bladder is conducted. The study is relied on the hypothesis that the androgen can down-regulate some key enzymes which are responsible for activating 4-ABP in the liver, and thus cause less formation of dG-C8-4-ABP in the liver and more in the bladder. This can eventually cause the higher incidence of bladder cancer in men than in women (gender disparity). The purpose of this project is to try to find out which are the key enzymes in the activating process. Previous studies have already excluded most of the candidate enzyme families but left enzyme Sulfotransferase which has several isoforms as our last hope. Only one sult isoform sult1d1 is shown to be positive in in-vitro experiments but unfortunately it was shown to be negative in in-vivo (knock-out) experiments. Other unknown Sult isoforms therefore could be involved with the gender disparity and waiting to be discovered. In Part III, a brief conclusion of the two projects in this dissertation is shown and followed with some expectations and future directions for both the use of DMS-MS in biodosimetry areas and the analysis of DNA adducts.
Book Synopsis Mass Spectrometry–Based Glycoproteomics and Its Clinic Application by : Haojie Lu
Download or read book Mass Spectrometry–Based Glycoproteomics and Its Clinic Application written by Haojie Lu and published by CRC Press. This book was released on 2021-09-20 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: As one of the most extensive and important protein post-translational modifications, glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently, researchers have focused on the need to characterize protein glycosylation sites, structures, and their degree of modification, to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative, quantitative, and data analysis, together with their clinical applications. Significant examples are discussed to illustrate the principles, laboratory protocols, and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine. Editor: Haojie Lu is a professor at Fudan University, specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.
Book Synopsis Systems Biology and Computational Proteomics by : Trey Ideker
Download or read book Systems Biology and Computational Proteomics written by Trey Ideker and published by Springer. This book was released on 2007-09-19 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the thoroughly refereed post-proceedings of two joint RECOMB 2006 satellite events: the Second Annual Workshop on Systems Biology, RSB 2006, and the First Biennial Workshop on Computational Proteomics, RCP 2006, held in San Diego, CA, USA in December 2006. The papers cover various aspects of systems biology and explore the use of computational mass spectrometry in various proteomic applications.
Book Synopsis Multidimensional Liquid Chromatography by : Steven A. Cohen
Download or read book Multidimensional Liquid Chromatography written by Steven A. Cohen and published by Wiley-Interscience. This book was released on 2008-04-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.
Book Synopsis Advancing Mass Spectrometry Methods for Glycosylation Analysis and Their Application to Disease-related Glyco-alteration Study by : Zhengwei Chen
Download or read book Advancing Mass Spectrometry Methods for Glycosylation Analysis and Their Application to Disease-related Glyco-alteration Study written by Zhengwei Chen and published by . This book was released on 2018 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) has evolved as a powerful tool for glycosylation analysis benefiting from its high speed, high resolution and sensitivity. It enables detailed structure characterization of glycans, glycosylation site and the peptide sequences of complex biological samples in a high-throughput. This dissertation is dedicated to developing MS-based analytical methods for the structure characterization and quantitative analysis of both released glycans and intact glycopeptides utilizing a bottom-up approach. We started by developing a hydrophilic interaction liquid chromatography (HILIC) coupled with matrix-assisted laser desorption/ionization-mass spectrometric imaging (MALDI-MSI) platform for improved quantitative analysis of N-glycans compared to MALDI-MS. Faced with the challenges of N-glycan structure complexity, capillary electrophoresis (CE) emerges as a robust separation technique affording very small amount of sample consumption, fast separation speed, and high resolving power. Therefore, we developed a CE-ESI-MS/MS based 6-plex aminoxy tandem mass tag (TMT) labeling method for quantitative analysis of protein N-glycosylation analysis from human serum, providing the ability of N-glycan quantitation across up to six samples. Improved resolution and quantification accuracy of the labeled human milk oligosaccharides (HMOs) isomers was also achieved by coupling CE with traveling wave ion mobility (TWIM)-CID-MS/MS. The advantage of the glycan-focused approach (glycomics) is that the wealthy structure information and glycan isomer separation could be achieved; however, this approach resulted in loss of the glycosylation site-specific information. Therefore, we developed a powerful glycoproteomics workflow for system-wide structure characterization and quantitation of intact glycopeptides. Improvements of this workflow include improved glycoprotein extraction, sequential enrichment strategies for improved N-/O- glycopeptide enrichment, hybrid novel fragmentation technique electron transfer and higher-energy collision dissociation (EThcD), multiplex quantitation enabled by our custom-made isobaric N, N-dimethyl leucine (DiLeu) tags, and automated FDR-based large-scale data analysis by Byonic. We have successfully applied this workflow to analyze complex biological samples for disease-related glyco-alteration study, including the glycosylation alteration in PKM2 knockout breast cancer cells vs. parental cells and glycosylation pattern alteration in AD patients.
Book Synopsis Ion Mobility-Mass Spectrometry by : Alison E Ashcroft
Download or read book Ion Mobility-Mass Spectrometry written by Alison E Ashcroft and published by Royal Society of Chemistry. This book was released on 2021-11-11 with total page 621 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, the use of ion mobility separation in combination with mass spectrometry analysis has developed significantly. This technique adds a unique extra dimension enabling the in-depth analysis of a wide range of complex samples in the areas of the chemical and biological sciences. Providing a comprehensive guide to the technique, each chapter is written by an internationally recognised expert and with numerous different commercial platforms to choose from, this book will help the end users understand the practicalities of using different instruments for different ion mobility purposes. The first section provides a detailed account of the fundamentals behind the technique and the current range of available instrumentation. The second section focusses on the wide range of applications that have benefitted from ion mobility – mass spectrometry and includes topics taken from current research in the pharmaceutical, metabolomics, glycomics, and structural molecular biology fields. The book is primarily aimed at researchers, appealing to practising chemists and biochemists, as well as those in the pharmaceutical and medical fields.
Book Synopsis Chiral Analysis by : Kenneth W. Busch
Download or read book Chiral Analysis written by Kenneth W. Busch and published by Elsevier. This book was released on 2011-10-13 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chiral Analysis covers an important area of analytical chemistry of relevance to a wide variety of scientific professionals. The target audience is scientific professionals with an undergraduate background in chemistry or a related discipline, specifically organic chemists, researchers in drug discovery, pharmaceutical researchers involved with process analysis or combinatorial libraries, and graduate students in chemistry. Chapters have been written with the nonspecialist in mind so as to be self-contained. * Broad coverage - spectroscopic and separation methods covered in a single volume * Up-to-date and detailed review of the various techniques available and/or under development in this field * Contributions from leading experts in the field
Book Synopsis Analysis of Protein Post-Translational Modifications by Mass Spectrometry by : John R. Griffiths
Download or read book Analysis of Protein Post-Translational Modifications by Mass Spectrometry written by John R. Griffiths and published by John Wiley & Sons. This book was released on 2016-11-07 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Book Synopsis Detection and Characterization of Glycans and Glycoconjugates Using Ion Mobility-mass Spectrometry by : Larissa Spell Fenn
Download or read book Detection and Characterization of Glycans and Glycoconjugates Using Ion Mobility-mass Spectrometry written by Larissa Spell Fenn and published by . This book was released on 2010 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Differential Ion Mobility Spectrometry by : Alexandre A. Shvartsburg
Download or read book Differential Ion Mobility Spectrometry written by Alexandre A. Shvartsburg and published by CRC Press. This book was released on 2008-12-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.
Book Synopsis Advances in Glycoproteomics and Glyco-biomarker Discovery Studies by : Francisca Owusu Gbormittah
Download or read book Advances in Glycoproteomics and Glyco-biomarker Discovery Studies written by Francisca Owusu Gbormittah and published by . This book was released on 2014 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of analytical technologies to investigate the glycoproteome of clinical relevant samples has improved over the last 10 years. These new developments aim to improve the identification and quantification of disease-specific glyco-biomarkers, which are present at low amounts in biological matrices. Glyco-biomakers have the potential to significantly contribute to cancer discovery studies in specific areas such as; early diagnosis, prognosis, monitor cancer recurrence and improve the low survival rate of cancer. In this thesis, we focused on the development and application of novel liquid affinity chromatography fractionation platforms integrated with nano-LC-MS/MS to characterize and quantify the glycoproteome as well as selected glyco-biomarker candidates of cancer samples. In chapter 1, brief background information covering glycoproteomics and glyco-biomarker discovery studies is presented. Specifically, protein glycosylation process and how the field of 'omics', which includes glycoproteomics, have revolutionized clinical glyco-biomarkers discovery are discussed. Further, various disease models, current sample fractionation strategies and analytical methodologies involved in glyco-biomarker development pipeline and their significance as well as their short falls are described. Reviewing biomarker validation and current bio-infomatics tools utilized in glycoproteomics discovery studies concludes chapter 1. Chapter 2 details the development of a novel multi-dimensional affinity liquid chromatography fractionation approach that combines the depletion of the top 12 abundant proteins and multi-lectin fractionation of the human plasma. Evaluating and validating the reproducibility, specificity and overall recoveries of the platform demonstrated the suitability of the developed method in glyco-biomarker discovery studies of clinical samples. After establishing this robust platform, it was applied in chapter 3 to comprehensively study the global glycoproteome profile of clear cell renal cell carcinoma plasma (ccRCC) samples to identify and characterize potential biomarkers for early detection of the disease. During this study, protein abundance alterations as well as glycan shifts were investigated to understand the sub-proteome of ccRCC. Chapter 4 focuses on the structural characterization of a glycoprotein (clusterin) that was identified during the ccRCC biomarker discovery studies. Clusterin has been implicated in ccRCC cancer progression however; its structure and biological function(s) are not yet well defined. Therefore, to have more structural insights into clusterin, the protein was immuno-affinity purified from ccRCC plasma followed by tandem mass spectrometry to profile glycoforms, "N"-glycosylation sites and quantify glycan amounts. We discovered that the levels of bi-antennary digalactosylated disialylated (A2G2S2) and core fucosylated bi-antennary digalactosylated disialylated (FA2G2S2) glycans differed significantly in the plasma of patients before and after curative nephrectomy of localized ccRCC. In chapter 5, a multi-lectin affinity chromatography platform previously developed in our laboratory was optimized and applied to investigate glycoproteins and non-glycoproteins present in pancreatic cyst fluid samples. This study was aimed at identifying potential candidate markers for early detection of malignant cyst (pancreatic cancer precursor). Our data showed the identification of proteins with significant differential expression in mucinous cysts (malignant cyst) compared to non-mucinous cysts (benign) of which one protein (periostin) associated with cancer progression was confirmed by immunoblotting assay. In the final chapter (chapter 6), we summarize and conclude our findings in this work and provide our perspective on the potential of glycoproteins in glyco-biomarker discovery studies.
Book Synopsis Analyzing Biomolecular Interactions by Mass Spectrometry by : Jeroen Kool
Download or read book Analyzing Biomolecular Interactions by Mass Spectrometry written by Jeroen Kool and published by John Wiley & Sons. This book was released on 2015-05-04 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
Book Synopsis Antibody Glycosylation by : Marija Pezer
Download or read book Antibody Glycosylation written by Marija Pezer and published by Springer Nature. This book was released on 2021-10-22 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes recent advances in antibody glycosylation research. Covering major topics relevant for immunoglobulin glycosylation - analytical methods, biosynthesis and regulation, modulation of effector functions - it provides new perspectives for research and development in the field of therapeutic antibodies, biomarkers, vaccinations, and immunotherapy. Glycans attached to both variable and constant regions of antibodies are known to affect the antibody conformation, stability, and effector functions. Although it focuses on immunoglobulin G (IgG), the most explored antibody in this context, and unravels the natural phenomena resulting from the mixture of IgG glycovariants present in the human body, the book also discusses other classes of human immunoglobulins, as well as immunoglobulins produced in other species and production systems. Further, it reviews the glycoanalytical methods applied to antibodies and addresses a range of less commonly explored topics, such as automatization and bioinformatics aspects of high-throughput antibody glycosylation analysis. Lastly, the book highlights application areas ranging from the ones already benefitting from antibody glycoengineering (such as monoclonal antibody production), to those still in the research stages (such as exploration of antibody glycosylation as a clinical or biological age biomarker), and the potential use of antibody glycosylation in the optimization of vaccine production and immunization protocols. Summarizing the current knowledge on the broad topic of antibody glycosylation and its therapeutic and biomarker potential, this book will appeal to a wide biomedical readership in academia and industry alike. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Book Synopsis Mass Spectrometry by : Edmond de Hoffmann
Download or read book Mass Spectrometry written by Edmond de Hoffmann and published by Wiley. This book was released on 2001-10-10 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a complete overview of the principles, theories and key applications of modern mass spectrometry in this introductory textbook. Following on from the highly successful first edition, this edition is extensively updated including new techniques and applications. All instrumental aspects of mass spectrometry are clearly and concisely described; sources, analysers and detectors. * Revised and updated * Numerous examples and illustrations are combined with a series of exercises to help encourage student understanding * Includes biological applications, which have been significantly expanded and updated * Also includes coverage of ESI and MALDI
Download or read book The Glycome written by Adeel Malik and published by CRC Press. This book was released on 2021-07-07 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive understanding of the enigmatic identity of the glycome, a complex but important area of research that has been largely ignored due to its complexity. The authors thoroughly deal with almost all aspects of the glycome, i.e., elucidation of the glycan identity enigma and its role in regulation of the cellular process, and in disease etiology. The book bridges the knowledge gap in understanding the glycome, from being a cell signature to its applications in disease etiology. In addition, it details many of the major insights regarding the possible role of the glycome in various diseases as a therapeutic marker. The book systematically covers the major aspects of the glycome, including the significance of substituting the diverse monosaccharide units to glycoproteins, the role of glycans in disease pathologies, and the challenges and advances in glycobiology. The authors stress the significance and huge encoding power of carbohydrates as well as provide helpful insights in framing the bigger picture. The Glycome: Understanding the Diversity and Complexity of Glycobiology details state-of-the-art developments and emerging challenges of glycome biology, which are going to be key areas of future research, not only in the glycobiology field but also in pharmaceutics.
Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao
Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.
