Fluorescent Gold Silver Alloy Core Silica Shell Nanoparticles For Bioimaging Applications

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Fluorescent Gold-silver Alloy Core Silica Shell Nanoparticles for Bioimaging Applications

Author : Siyu Tu
Publisher :
ISBN 13 :
Total Pages : 107 pages
Book Rating : 4.:/5 (114 download)

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Book Synopsis Fluorescent Gold-silver Alloy Core Silica Shell Nanoparticles for Bioimaging Applications by : Siyu Tu

Download or read book Fluorescent Gold-silver Alloy Core Silica Shell Nanoparticles for Bioimaging Applications written by Siyu Tu and published by . This book was released on 2016 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials have been a popular research field for decades and scientists are always trying to develop novel materials. This can be achieved with a hybrid of existing material to either significantly improve the performance for certain applications or generate new properties which can be exploited for new applications. Two perfect examples are gold-silver (Au/Ag) alloy nanoparticles (NPs) with strong and tunable extinction spectra in the visible range and superluminescent NPs with a metallic core and dye-doped silica shell. Both of them can be used as contrast agents for biomedical imaging applications. This research is based on the combination of these two types of NPs: the demonstration of a multiplexed bioimaging application with fluorescent NPs composed of a Au/Ag alloy core and a fluorophore-doped silica shell(Au/Ag SiO2). It has long been known that immunofluorescence, as the standard technique to label cells, has some drawbacks such as intensity limit, limited multiplexing and loss of signal due to photobleaching. Plasmonic NPs do not photobleach and do not lose their scattering signal. The Au/Ag alloy NPs with a core-shell structure not only exploit the merits of a tunable scattering signal from the core but also the metal enhanced fluorescence (MEF) effect from the shell near a metallic surface. In this research, we have fabricated the hybrid NPs and applied them in a multimodal platform to detect both scattering and fluorescence signals in order to improve the multiplexing capability. During the master study, Au/Ag SiO2 NPs were successfully fabricated with high reproducibility. Different fluorophores with different emission wavelengths were incorporated in the silica shell of Au/Ag SiO2 NPs resulting in a stronger fluorescence signal in the presence of a metallic core. An etching effect of the metallic core was noted during the silica shell growth, creating a spacer between the surface of the metallic core and silica shell resulting in a slight blueshift of the plasmonic peak. This effect is more severe for silver-rich cores. Four types of NPs were prepared, with two different Au/Ag composition ratios (pure Ag and 50/50 Au/Ag) and two different fluorophores (fluorescein and rhodamine B) were incorporated into the silica shell for a bimodal multiplexed imaging test in scattering and fluorescence. A proportion of 86% of NPs on a glass slide can be identified and a similar result was obtained for NPs on cells.

Multifunctional Core-shell Silica Particles

Author : Hooisweng Ow
Publisher :
ISBN 13 :
Total Pages : 314 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis Multifunctional Core-shell Silica Particles by : Hooisweng Ow

Download or read book Multifunctional Core-shell Silica Particles written by Hooisweng Ow and published by . This book was released on 2005 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Gold and Silver Nanoparticles

Author : Suban K. Sahoo
Publisher : Elsevier
ISBN 13 : 0323994555
Total Pages : 454 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Gold and Silver Nanoparticles by : Suban K. Sahoo

Download or read book Gold and Silver Nanoparticles written by Suban K. Sahoo and published by Elsevier. This book was released on 2023-01-20 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gold and Silver Nanoparticles: Synthesis and Applications provides detailed information on the preparation and utilization of Au- and Ag-based nanoparticles in a range of novel areas. Gold and silver nanoparticles offer a range of interesting properties, including unique size-dependent optoelectronic properties, chemical stability and biocompatibility, ease of synthesis and surface modification, excellent resistance to corrosion, and catalytic properties, hence paving the way to a wide range of cutting-edge applications with continual advances and innovations. Sections introduce gold and silver nanoparticles, fundamental theory, synthesis, and characterization techniques before focusing on requirements and preparation methods. Specific applications areas, such as surface-enhanced Raman spectroscopy (SERS), sensing and biosensing, imaging, drug and gene delivery, disease diagnosis, catalysis, and optoelectronic device fabrication are covered. Finally, synthesis and applications of platinum- and palladium-based nanoparticles are discussed. This is a valuable resource for researchers and advanced students across nanoscience and nanotechnology, chemistry, and materials science, as well as scientists, engineers, and R&D professionals with an interest in noble metal nanomaterials for a range of industrial applications. - Explains theory, synthesis, characterization, and properties of Au- and Ag- based nanoparticles - Explores a range of novel applications across biomedicine, optoelectronics, and other areas - Analyzes the latest developments in the field and considers noble metal nanoparticles beyond gold and silver

Rational Design of SERS Active Silica Coated Silver Nanoparticles as a Versatile Platform for Semi-quantitative Bio-imaging

Author : Louise Rocks
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)

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Book Synopsis Rational Design of SERS Active Silica Coated Silver Nanoparticles as a Versatile Platform for Semi-quantitative Bio-imaging by : Louise Rocks

Download or read book Rational Design of SERS Active Silica Coated Silver Nanoparticles as a Versatile Platform for Semi-quantitative Bio-imaging written by Louise Rocks and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years there has been an increased demand for real-time detection of specific biological species and interactions within a meaningful environment. Surface enhanced Raman scattering (SERS) from metallic nanoparticles represents an approach under development with added advantages over fluorescence. One such benefit is the narrower vibrational bands in SERS compared to fluorescence which increases potential for simultaneous detection of more than one species. The use of metallic nanoparticles in Raman enhancement is hindered by non-specific adsorption of contaminating species and uncontrolled aggregation in vivo. Silica encapsulation of the nanoparticles results in a biocompatible and robust substrate with consistent surface chemistry. While gold nanoparticles have been shelled in silica by many different methods, transfer of encapsulation methods to silver has not been possible due to the reduced chemical stability of silver. New surface chemistries are therefore required to achieve reproducible, silica encapsulated silver nanoparticles. Tri-functional molecules were synthesised which stabilise the silver nanoparticle core, act as (resonance) Raman reporters and provide suitable precursors for subsequent silica encapsulation. Subsequent bio-functionalisation of these nanoparticles, by covalent attachment of proteins, generates SERS active bioimaging nanoparticles. Raman mapping of biological samples has been used for ex vivo detection and imaging of the inflammatory marker tumour necrosis factor. Semi-quantitative discrimination, of cerebral tissue sections, was achieved between mice infected with experimental cerebral malaria and uninfected controls. These results were produced using both 632.8 nm and 514.5 nm excitation wavelengths. In this work, a sensitive SERS based approach to biomolecule imaging has been developed. Successful targeting of TNF using silver nanoparticles, and excitation wavelengths in the visible region, may provide advantages over the use of gold in a wide range of SERS based applications.

Fluorescence and Magnetic Resonance Enabled Silica Nanoparticles for Bio-imaging Applications

Author : Parvesh Sharma
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (129 download)

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Book Synopsis Fluorescence and Magnetic Resonance Enabled Silica Nanoparticles for Bio-imaging Applications by : Parvesh Sharma

Download or read book Fluorescence and Magnetic Resonance Enabled Silica Nanoparticles for Bio-imaging Applications written by Parvesh Sharma and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Metal-Enhanced Fluorescence

Author : Chris D. Geddes
Publisher : John Wiley & Sons
ISBN 13 : 0470642785
Total Pages : 655 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Metal-Enhanced Fluorescence by : Chris D. Geddes

Download or read book Metal-Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.

Gold-silver Core-shell Nanoparticle Growth

Author : Austin James Ethridge
Publisher :
ISBN 13 :
Total Pages : 212 pages
Book Rating : 4.:/5 (17 download)

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Book Synopsis Gold-silver Core-shell Nanoparticle Growth by : Austin James Ethridge

Download or read book Gold-silver Core-shell Nanoparticle Growth written by Austin James Ethridge and published by . This book was released on 2018 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Novel Nanomaterials

Author : George Kyzas
Publisher : BoD – Books on Demand
ISBN 13 : 1789230888
Total Pages : 372 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Novel Nanomaterials by : George Kyzas

Download or read book Novel Nanomaterials written by George Kyzas and published by BoD – Books on Demand. This book was released on 2018-04-18 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Nanomaterials" is a special topic of recent research and is a milestone of nanoscience and nanotechnology. Nanoscale materials are a series of substances/compounds, in which at least one dimension has smaller size than 100 nm. Nanomaterials have a broad area of development, which is growing rapidly day by day. Their impact on commercial applications as well as on the respective academia and education is huge. The basic points of this book can be divided into synthesis of nanomaterials and their applications. For example, special mention is about metal-oxide nanostructures, nanocomposites, and polymeric nanomaterials. Also, synthesis, characterizations, various processes, fabrications and some promising applications are also developed and analyzed.

Nanoalloys

Author : Florent Calvo
Publisher : Newnes
ISBN 13 : 0123946166
Total Pages : 433 pages
Book Rating : 4.1/5 (239 download)

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Book Synopsis Nanoalloys by : Florent Calvo

Download or read book Nanoalloys written by Florent Calvo and published by Newnes. This book was released on 2013-03-12 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoalloys: From Fundamentals to Emergent Applications presents and discusses the major topics related to nanoalloys at a time when the literature on the subject remains scarce. Particular attention is paid to experimental and theoretical aspects under the form of broad reviews covering the most recent developments. The book is organized into 11 chapters covering the most fundamental aspects of nanoalloys related to their synthesis and characterization, as well as their theoretical study. Aspects related to their thermodynamics and kinetics are covered as well. The coverage then moves to more specific topics, including optics, magnetism and catalysis, and finally to biomedical applications and the technologically relevant issue of self-assembly.With no current single reference source on the subject, the work is invaluable for researchers as the nanoscience field moves swiftly to full monetization. - Encapsulates physical science of structure, properties, size, composition and ordering at nanoscale, aiding synthesis of experimentation and modelling - Multi-expert and interdisciplinary perspectives on growth, synthesis and characterization of bimetallic clusters and particulates supports expansion of your current research activity into applications - Synthesizes concepts and draws links between fundamental metallurgy and cutting edge nanoscience, aiding interdisciplinary research activity

Silver and Gold

Author : Matthew Lawrence Weinlander
Publisher :
ISBN 13 :
Total Pages : 124 pages
Book Rating : 4.:/5 (65 download)

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Book Synopsis Silver and Gold by : Matthew Lawrence Weinlander

Download or read book Silver and Gold written by Matthew Lawrence Weinlander and published by . This book was released on 2010 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology is a new scientific field with many exciting potential applications in medicine, energy development, and daily life. However, due to their size, nanoparticles have many properties that are distinct from their bulk counterparts. Like many new technologies, the environmental implications of its use have not been thoroughly investigated. It has been shown that silver nanoparticles (AgNPs) cause structural abnormalities in developing Zebrafish (danio rerio) (Mohrmann, Lawrence University 2008). Using a TUNEL assay, it becomes apparent that there are no significant differences between the developmental apoptosis of embryos treated with AgNPs and those without treatment. Tamayo Uechi, et. al. have shown that knockdowns of certain ribosomal proteins results in the same phenotypic defects displayed in the nanoparticle-treated embryos. It is possible that interactions between silver nanoparticles (~1 nm) (http://purestcolloids.com/mesoworld.htm) and these ribosomes (~25 nm) (Moore, 2004) are the source of the abnormalities. RT-PCR (real time polymerase chain reaction) shows that the expression of at least one ribosomal protein gene (ribosomal protein 3) is attenuated by the presence of silver nanoparticles. A translation assay shows promise in determining if the nanoparticles also affect translation, but more data must be collected. A potentially useful application of nanoparticles is in the use of fluorescent nanoparticles (quantum dots) in biomedical imaging. The common nanoparticle that is used in these capacities is Cadmium Selenide (CdSe). The concern with these nanoparticles is their toxicity to humans. However, gold nanoparticles have been repeatedly shown to be nontoxic. This suggests that fluorescent gold nanoparticles are a potential nontoxic alternative for medical applications. The question that remains, then, is how best to manufacture these fluorescent gold nanoparticles. By adding dendrimer (regular, highly-branched monomers that leads to a tree-like generational structure), HAuCl4, and a reducing agent (either trisodium citrate, sodium borohydride, or sodium ascorbate) to water, fluorescent gold nanoparticles are synthesized. Not only are these nanoparticles fluorescent, but their size and shape can be predetermined as well by adding variable amounts of methanol or polyethylene glycol. Both the size and shape of fluorescent gold nanoparticles are important variables to consider, as each may affect the fluorescence of the nanoparticles. Along with the size and shape, the addition of dendrimer could influence fluorescence. With so many variables that can be modified, gold nanoparticle synthesis seems to be an ideal introduction to the nanoworld for introductory chemistry classes. The preliminary data suggest the synthesis of different gold nanoparticle shapes and the synthesis of fluorescent gold nanoparticles. Testing the physical properties of the fluorescence and shape of the gold nanoparticles is an ongoing endeavor.

Development of New Fluorescent Silica and Multifunctional Nanoparticles for Bio-imaging and Diagnostics

Author : Arnaud Lemelle
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (86 download)

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Book Synopsis Development of New Fluorescent Silica and Multifunctional Nanoparticles for Bio-imaging and Diagnostics by : Arnaud Lemelle

Download or read book Development of New Fluorescent Silica and Multifunctional Nanoparticles for Bio-imaging and Diagnostics written by Arnaud Lemelle and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Silica nanoparticles are effective fluorophore carriers with high potential in imaging, diagnostics, and therapy. The particles are resistant to drastic change of environmental conditions (pH, temperature etc.) and insulate the dyes so as to protect them from photobleaching. Silica chemistry is also versatile and affords an easy modification of the particle composition and surface to integrate targeting ligands or to integrate other nanoparticles. Regardless of their advantages, there exists a lack of dye diversity in the literature that is connected to a low affinity for potential tools for biology and medicine. This thesis describes the developmentof an alternative method for the synthesis of fluorescent silica nanoparticles and their modification to incorporate iron oxide and gold.

Metal Semiconductor Core-shell Nanostructures for Energy and Environmental Applications

Author : Raju Kumar Gupta
Publisher : Elsevier
ISBN 13 : 0128124458
Total Pages : 220 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Metal Semiconductor Core-shell Nanostructures for Energy and Environmental Applications by : Raju Kumar Gupta

Download or read book Metal Semiconductor Core-shell Nanostructures for Energy and Environmental Applications written by Raju Kumar Gupta and published by Elsevier. This book was released on 2017-04-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metal Semiconductor Core-Shell Nanostructures for Energy and Environmental Applications provides a concise, scholarly overview of current research into the characterization of metal semiconductor core-shell nanostructures; the book shows how their properties can be best used in energy and environmental applications, particularly for solar cell and catalysis application. Coverage is also given to the effect of metal nanoparticle for charge generation or charge separation. The book is a valuable resource for academic researchers working in the areas of nanotechnology, sustainable energy and chemical engineering, and is also of great use to engineers working in photovoltaic and pollution industries. - Includes a clear method for synthesis of core-shell nanomaterials - Explores how metal semiconductor core-shell nanostructures can be used to improve the efficiency of solar cells - Explains how the characteristics of metal semiconductor core-shell nanostructures make them particularly useful for sustainable energy and environmental applications

Preparation, Transformation, and Optical Applications of Silver-gold Bimetallic Core-shell Nanoparticles

Author : Brett W. Boote
Publisher :
ISBN 13 :
Total Pages : 150 pages
Book Rating : 4.:/5 (863 download)

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Book Synopsis Preparation, Transformation, and Optical Applications of Silver-gold Bimetallic Core-shell Nanoparticles by : Brett W. Boote

Download or read book Preparation, Transformation, and Optical Applications of Silver-gold Bimetallic Core-shell Nanoparticles written by Brett W. Boote and published by . This book was released on 2013 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Characterisation and Peparation [sic] of Fluorescent Magetic [sic] Silica Nanoparticles for Drug Delivery

Author : Niancen Peng
Publisher :
ISBN 13 :
Total Pages : 126 pages
Book Rating : 4.:/5 (743 download)

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Book Synopsis Characterisation and Peparation [sic] of Fluorescent Magetic [sic] Silica Nanoparticles for Drug Delivery by : Niancen Peng

Download or read book Characterisation and Peparation [sic] of Fluorescent Magetic [sic] Silica Nanoparticles for Drug Delivery written by Niancen Peng and published by . This book was released on 2010 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Magnetic silica nanoparticles with the core-shell structure were prepared by Stober method. There has been dramatic interest in the use of these particles for drug delivery in the recent years, however, little is known about their stability in aqueous environments. The aim of the current project is to examine their fate in water, to optimize their surface properties and finally to label them with a fluorescent dye for tracking purposes. Magnetic particles served as the core, and the silica shell was prepared from tetraethyl orthosilicate (TEOS). Changing the amount and the surface properties of the core, and varying the concentration of the shell or the solvent (ethanol) were conducted to prepare different types of magnetic silica nanoparticles. Different methods were used to characterize the nanoparticles, with the aim of finding the best conditions to prepare stable, uniform and narrow small sized nanoparticles. These measurements included: dynamic light scattering (DLS) for measuring the particles size, average count rate and to perform dissolution studies, differential scanning calorimetry (DSC) for thermal analysis of the dissolution process, UV/Vis Spectroscopy to study the turbidity, transmission electron microscopy (TEM), and measurement for zeta potential and pH. -- The first parameter studied was the magnetic core (Fe304). Coating these particles with citric acid seemed to improve the silica coating. This was attributed to the complexation between the TEOS and the citric acid, which resulted in uniform coating with silica. Another factor studied was the concentration of the precursor (TEOS). The results showed that the particle size increased with increasing the amount of TEOS. The latter was also accompanied by better stability (against dissolution) in water.

Fluorescent Silica Nanoparticles

Author : Song Liang
Publisher :
ISBN 13 :
Total Pages : 148 pages
Book Rating : 4.:/5 (648 download)

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Book Synopsis Fluorescent Silica Nanoparticles by : Song Liang

Download or read book Fluorescent Silica Nanoparticles written by Song Liang and published by . This book was released on 2007 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Silica Coating of Gold Nanoparticles

Author : Milind Laxman Surve
Publisher :
ISBN 13 :
Total Pages : 128 pages
Book Rating : 4.:/5 (548 download)

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Book Synopsis Silica Coating of Gold Nanoparticles by : Milind Laxman Surve

Download or read book Silica Coating of Gold Nanoparticles written by Milind Laxman Surve and published by . This book was released on 2003 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The project deals with the synthesis and characterization of gold-silica core-shell nanoparticles. It uses solution chemistry to polymerize silica on gold surface and form a coating of desirable thickness. . . However, this research specifically deals with coating gold nanoparticles of size less than 15 nm, like 10 nm, 5 nm and 2 nm."--Introduction, p. 2.

Fluorescent Nanodiamonds

Author : Huan-Cheng Chang
Publisher : John Wiley & Sons
ISBN 13 : 1119477085
Total Pages : 294 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Fluorescent Nanodiamonds by : Huan-Cheng Chang

Download or read book Fluorescent Nanodiamonds written by Huan-Cheng Chang and published by John Wiley & Sons. This book was released on 2018-11-12 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most comprehensive reference on fluorescent nanodiamond physical and chemical properties and contemporary applications Fluorescent nanodiamonds (FNDs) have drawn a great deal of attention over the past several years, and their applications and development potential are proving to be manifold and vast. The first and only book of its kind, Fluorescent Nanodiamonds is a comprehensive guide to the basic science and technical information needed to fully understand the fundamentals of FNDs and their potential applications across an array of domains. In demonstrating the importance of FNDs in biological applications, the authors bring together all relevant chemistry, physics, materials science and biology. Nanodiamonds are produced by powerful cataclysmic events such as explosions, volcanic eruptions and meteorite impacts. They also can be created in the lab by high-pressure high-temperature treatment of graphite or detonating an explosive in a reactor vessel. A single imperfection can give a nanodiamond a specific, isolated color center which allows it to function as a single, trapped atom. Much smaller than the thickness of a human hair, a nanodiamond can have a huge surface area that allows it to bond with a variety of other materials. Because of their non-toxicity, nanodiamonds may be useful in biomedical applications, such as drug delivery and gene therapy. The most comprehensive reference on a topic of rapidly increasing interest among academic and industrial researchers across an array of fields Includes numerous case studies and practical examples from many areas of research and industrial applications, as well as fascinating and instructive historical perspectives Each chapter addresses, in-depth, a single integral topic including the fundamental properties, synthesis, mechanisms and functionalisation of FNDs The first book published by the key patent holder with his research group in the field of FNDs Fluorescent Nanodiamonds is an important working resource for a broad range of scientists and engineers in industry and academia. It will also be a welcome reference for instructors in chemistry, physics, materials science, biology and related fields.