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Author : Tsz-kit Chik
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (8 download)
Download or read book Fabrication of Multi-component Tissue for Intervertebral Disc Tissue Engineering written by Tsz-kit Chik and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Tsz-kit Chik
Publisher :
ISBN 13 :
Total Pages : 394 pages
Book Rating : 4.:/5 (8 download)
Download or read book Fabrication of Multi-component Tissue for Intervertebral Disc Tissue Engineering written by Tsz-kit Chik and published by . This book was released on 2012 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Tsz-Kit Chik
Publisher :
ISBN 13 : 9781361290996
Total Pages : pages
Book Rating : 4.2/5 (99 download)
Download or read book Fabrication of Multi-Component Tissue for Intervertebral Disc Tissue Engineering written by Tsz-Kit Chik and published by . This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Fabrication of Multi-component Tissue for Intervertebral Disc Tissue Engineering" by Tsz-kit, Chik, 戚子傑, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Intervertebral disc tissue engineering is challenging because it involves the integration of multiple tissues with distinct structures and compositions such as lamellar annulus fibrosus, gel'like nucleus pulposus and cartilage endplate. Each of them has different compositions and different structures. It is hypothesized that integration of tissues can be enhanced with appropriate mechanical and biological stimuli. Meanwhile, effect of torsional stimulus on cell re'orientation in mesenchymal stem cell'collagen tubular constructs is investigated in this study. Furthermore, it is proposed that these findings can be used to fabricate a multicomponent unit for intervertebral disc tissue engineering. It has been demonstrated that mechanical and biological stimuli can stabilize the interface between osteogenic and chondrogenic differentiated constructs with enhanced ultimate tensile stress while the phenotype of osteogenic and chondrogenic differentiated constructs were maintained. Scanning electronic microscopic images have shown aligned collagen fibrils and presence of calcium at the interface, indicating the possibility of the formation of a calcified zone. In addition, it is proven that torsional stimulus triggered re'orientation of mesenchymal stem cells in collagen lamellae towards a preferred angle. Cell alignments were confirmed by using a MatLab'based program to analyze the actin filament and the cell alignment via Phalloidin and Hematoxylin staining, respectively. Cells and actin filaments were inclined around 30o from the vertical axis, while cells and filaments in the control group (static loading) aligned along the vertical axis. Furthermore, a double'layers bioengineered unit was fabricated, with intact osteogenic differentiated parts at both ends. Comparatively higher cell density was observed at the interface between layers, demonstrating the interactions between layers, while the phenotype of each part was maintained in 14 days culture. This study concludes that a multi'components bioengineered unit with preferred cell alignments can be fabricated. This provides new insights to future development of bioengineered spinal motion segment for treating late stage disc degeneration. DOI: 10.5353/th_b4784944 Subjects: Intervertebral disk prostheses Tissue engineering - Materials
Author : Tsz-Hang Andrew Choy
Publisher : Open Dissertation Press
ISBN 13 : 9781361308080
Total Pages : pages
Book Rating : 4.3/5 (8 download)
Download or read book Fabrication of a Biphasic Scaffold for Tissue Engineering of Intervertebral Disc written by Tsz-Hang Andrew Choy and published by Open Dissertation Press. This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Fabrication of a Biphasic Scaffold for Tissue Engineering of Intervertebral Disc" by Tsz-hang, Andrew, Choy, 蔡子鏗, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Current treatments to intervertebral disc degeneration alter spine biomechanics and have complications. Tissue engineering offers an approach to regenerate a biological disc that provides flexibility and stability to, and integrates with the spine. To date, a scaffold that mimics the extracellular matrix composition and mechanical strength of a native disc is lacked. In this project, a biphasic scaffold was fabricated using glycosaminoglycan (GAG) and collagen, the prevalent ma-trix components in a native disc. It also adapted the structure of the disc, with la-mellae of collagen surrounding a collagen-GAG (CG) core. The first part of this project studied chemical modification of CG and evaluated the physiochemical and biological properties of modified CGs. As only loosely bound by GAG under physiological environment, collagen was modified by deamination, methylation and amination, and yielded Deaminated, Methylated and Aminated CGs upon co-precipitation with GAG. While GAG was mostly lost within 1 day in Untreated and Deaminated CGs, 20% and 40% GAG was retained after 6 days in Methylated and Aminated CGs respectively. In cell-seeded Aminated CG, over 60% GAG was retained after 8 days. Aminated CG, having the highest GAG/HYP of 4.5, best simulated the GAG-rich nucleus pulposus tissue. In ultrastructural analysis, Aminated CG consisted of abundant granular sub-stances that resembled the nucleus pulposus. Despite the differential initial number adhered to the CG scaffolds, human mesenchymal stem cells (hMSCs) had over 90% viability at all time points. Cell morphology was distinct, being round in Untreated and Methylated CGs but elongated in Deaminated and Aminated ones. The adhesion of hMSCs via collagen receptor, integrin alpha2beta1, was observed in all CG scaffolds, while adhesion via general matrix receptor, integrin alphaV, was extensive in all but Aminated CG. Based on improved GAG incor-poration and retention, which approximate the matrix composition of nucleus pulposus, Aminated CG was chosen as the core of the biphasic scaffold. The second part of this project studied lamination in biphasic disc scaffold and evaluated its mechanical properties in creep, recovery and dynamic loadings. A process was optimized to encapsulate a CG under physiological condition whilst producing an intact collagen gel, which allowed the CG to retain more GAGs and to be confined by the annulus structurally as was in the disc. This encasing approach was repeated for multiple lamellae, one lamella per day. Scaffolds with more lamellae had increased viscous compliance in creep and recovery, which was explained by the less laminated scaffolds being overloaded. Another lamination approach replaced most encasing lamellae with coiling ones. Despite low sample size, it was shown that this combined approach produced scaffolds with lower elastic and viscous compliances and longer equilibrating time in both creep and recovery, and higher complex modulus under dynamic loading. Full recovery was not achieved by any scaffold. This study demonstrated that a biphasic disc scaffold, made of GAG and collagen, contained similar matrix components to native disc, was almost mechanically comparable to the disc, and was cyto-compatible. It paved way towards tissue engineering of intervertebral disc and the intervertebral disc motion segment. DOI: 10.5353/th_b4979945 Subjects: Tissu
Author : Tsz-hang Choy (Andrew)
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (828 download)
Download or read book Fabrication of a Biphasic Scaffold for Tissue Engineering of Intervertebral Disc written by Tsz-hang Choy (Andrew) and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Bertrand Guillotin
Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128090537
Total Pages : 26 pages
Book Rating : 4.1/5 (28 download)
Download or read book Biofabrication written by Bertrand Guillotin and published by Elsevier Inc. Chapters. This book was released on 2013-03-18 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser-assisted bioprinting (LAB) is an emerging technology in the field of tissue engineering. Its physical mechanism makes it possible to print cells and liquid materials with a cell-level resolution. By giving tissue engineers control over cell density and organization of 3D tissue constructs, LAB holds much promise for fabricating living tissues with physiological functionality. After introducing the rationale of applying LAB to tissue engineering, we present exhaustively the physical parameters related to the laser-induced forward transfer technique (LIFT), which is implemented in LAB. These parameters are critical to controlling the cell printing process and must work together to print viable cell patterns with respect to cell-level histological organization and to high-throughput manufacturing. After describing the experimental requirements that should be considered to fabricate 3D tissues by LAB, we present some of the main breakthroughs, including multicomponent printing, 3D printing approaches, and bioprinting in vivo that may serve in tissue engineering and regenerative medicine.
Author : Tsz-hang Choy (Andrew)
Publisher :
ISBN 13 :
Total Pages : 146 pages
Book Rating : 4.:/5 (828 download)
Download or read book Fabrication of a Biphasic Scaffold for Tissue Engineering of Intervertebral Disc written by Tsz-hang Choy (Andrew) and published by . This book was released on 2012 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Paulo Rui Fernandes
Publisher : Springer
ISBN 13 : 303015372X
Total Pages : 86 pages
Book Rating : 4.0/5 (31 download)
Download or read book New Developments in Tissue Engineering and Regeneration written by Paulo Rui Fernandes and published by Springer. This book was released on 2019-03-27 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a new contribution for the field of Tissue Engineering with a focus on the development of mathematical and computational methods that are relevant to understand human tissues, as well to model, design, and fabricate optimized and smart scaffolds. The multidisciplinary character of this field has motivated contributions from different areas with a common objective to replace damaged tissues and organs by healthy ones. This work treats tissue healing approaches, mathematic modelling for scaffold design and bio fabrication methods, giving the reader a broad view of the state of the art in Tissue Engineering. The present book contains contributions from recognized researchers in the field, who were keynote speakers in the Fourth International Conference on Tissue Engineering, held in Lisbon in 2015, and covering different aspects of Tissue Engineering. The book is strongly connected with the conference series of ECCOMAS Thematic Conferences on Tissue Engineering, an event that brings together a considerable number of researchers from all over the world, representing several fields of study related to Tissue Engineering.
Author : 王[Chong] (Researcher on mechanical engineering)
Publisher :
ISBN 13 :
Total Pages : 437 pages
Book Rating : 4.:/5 (873 download)
Download or read book Electrospun Multicomponent and Multifunctional Nanofibrous Tissue Engineering Scaffolds written by 王[Chong] (Researcher on mechanical engineering) and published by . This book was released on 2013 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Phuc Van Pham
Publisher : BoD – Books on Demand
ISBN 13 : 9535136151
Total Pages : 302 pages
Book Rating : 4.5/5 (351 download)
Download or read book Mesenchymal Stem Cells written by Phuc Van Pham and published by BoD – Books on Demand. This book was released on 2017-11-29 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal Stem Cells: Isolation, Characterization, and Applications thoroughly presents the isolation, characterization, and some applications of mesenchymal stem cells in the clinic. The book has two parts: "Isolation and Characterization" and "Clinical Perspectives and Applications." In Part I, the subsequent chapters introduce some techniques in isolation, characterization, and purification of mesenchymal stem cells in different tissues. In Part II, some applications of mesenchymal stem cells in the popular diseases, which include cartilage regeneration, spinal cord injury, and osteoarthritis, are discussed. This book provides a succinct yet comprehensive overview of mesenchymal stem cells for advanced students, graduate students, and researchers.
Author : Julia M Polak
Publisher : World Scientific
ISBN 13 : 1908978902
Total Pages : 947 pages
Book Rating : 4.9/5 (89 download)
Download or read book Advances In Tissue Engineering written by Julia M Polak and published by World Scientific. This book was released on 2008-08-20 with total page 947 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Tissue Engineering is a unique volume and the first of its kind to bring together leading names in the field of tissue engineering and stem cell research. A relatively young science, tissue engineering can be seen in both scientific and sociological contexts and successes in the field are now leading to clinical reality. This book attempts to define the path from basic science to practical application. A contribution from the UK Stem Cell Bank and opinions of venture capitalists offer a variety of viewpoints, and exciting new areas of stem cell biology are highlighted. With over fifty stellar contributors, this book presents the most up-to-date information in this very topical and exciting field./a
Author : Sibylle Grad
Publisher : Morgan & Claypool Publishers
ISBN 13 : 9781608454655
Total Pages : 104 pages
Book Rating : 4.4/5 (546 download)
Download or read book Cells and Biomaterials for Intervertebral Disc Regeneration written by Sibylle Grad and published by Morgan & Claypool Publishers. This book was released on 2010-04-04 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Disorders related to the intervertebral disc (IVD) are common causes of morbidity and of severe life quality deterioration. IVD degeneration, although in many cases asymptomatic, is often the origin of painful neck and back diseases. In Western societies IVD related pain and disability account for enormous health care costs as a result of work absenteeism and thus lost production, disability benefits, medical and insurance expenses. Although only a small percentage of patients with disc disorders finally will undergo surgery, spinal surgery has been one of the fastest growing disciplines in the musculoskeletal field in recent years. Nevertheless, current treatment options are still a matter of controversial discussion. In particular, they hardly can restore normal spine biomechanics and prevent degeneration of adjacent tissues. While degeneration affects all areas of the IVD, the most constant and noticeable changes occur in the gel-like central part, the nucleus pulposus (NP). Recent emphasis has therefore been put in biological ways to regenerate the NP; however, there are a number of obstacles to overcome, considering the exceptional biological and biomechanical environment of this tissue. Different biological approaches such as molecular, gene, and cell based therapies have been investigated and have shown promising results in both in vitro and in vivo studies. Nonetheless, considerable hurdles still exist in their application for IVD regeneration in human patients. The choice of the cells and the choice of the cell carrier suitable for implantation pose major challenges for research and development activities. This lecture recapitulates the basics of IVD structure, function, and degeneration mechanisms. The first part reviews the recent progress in the field of disc and stem cell based regenerative approaches. In the second part, most appropriate biomaterials that have been evaluated as cell or molecule carrier to cope with degenerative disc disease are outlined. The potential and limitations of cell- and biomaterial-based treatment strategies and perspectives for future clinical applications are discussed. Table of Contents: Cell Therapy for Nucleus Pulposus Regeneration / Recent Advances in Biomaterial Based Tissue Engineering for Intervertebral Disc Regeneration
Download or read book FABRICATION OF PREVASCULARIZED CELL-DERIVED EXTRACELLULAR MATRIX BASED BIOMIMETIC TISSUE CONSTRUCTS FOR MULTIPLE TISSUE ENGINEERING written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : Tissue engineering and regenerative medicine has been vast developing since the past decade. A preformed functional vascular network provides effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered tissues. As one of the important mural cells, human mesenchymal stem cells (hMSCs) not only stabilize the engineered microvessel network, but also preserve their multi-potency when grown under optimal culture conditions. A prevascularized hMSC/ECM sheet fabricated by the combination of hMSCs, ECs and a naturally derived nanofibrous extracellular matrix (ECM) scaffold offers great opportunity for engineering mechanically strong and completely biological 3D prevascularized tissues. Moreover, for regeneration of highly organized tissues such as skeletal, cardiac, and neural tissues, it is critical to create aligned vasculatures within the constructs, which not only provide inlets/outlets for ease of vessel anastomosis, but also play important roles in tissue functional performance. The topographical stimulation enhanced the structure, density, and length of the vascular networks by promoting pro-angiogenic growth factor secretion and guiding neovasculature orientation. The matrix remodeling enzymatic activity assessment revealed that CD166 mediated MMP-2 activation played a major role in angiogenesis and vasculature remodeling direction. Preliminary studies have shown that hMSCs can be transplanted to improve nerve regeneration. Hypoxia pretreated hMSCs had an improved potential for peripheral nerve regeneration. The future works can focus on studying the neural protective functions of MSCs, and how prevascularized scaffolds can restore the oxygen supply towards the hypoxic region upon ischemia or injury. In summary, prevascularized nanofibrous ECM constructs can serve as building blocks to engineer 3D oriented tissues with aligned vasculatures, while preserving the therapeutic potential of bulk hMSCs. Together, this study paved the way for engineering large organs, and provided a perspective on replicate native cell/ECM/vessel structure to benefit regenerative medicine.
Author : Antonella Motta
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (112 download)
Download or read book Scaffolds in Tissue Engineering written by Antonella Motta and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Gilson Khang
Publisher : CRC Press
ISBN 13 : 1315341026
Total Pages : 847 pages
Book Rating : 4.3/5 (153 download)
Download or read book Handbook of Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine written by Gilson Khang and published by CRC Press. This book was released on 2017-06-26 with total page 847 pages. Available in PDF, EPUB and Kindle. Book excerpt: Millions of patients suffer from end-stage organ failure or tissue loss annually, and the only solution might be organ and/or tissue transplantation. To avoid poor biocompatibility–related problems and donor organ shortage, however, around 20 years ago a new, hybridized method combining cells and biomaterials was introduced as an alternative to whole-organ and tissue transplantation for diseased, failing, or malfunctioning organs—regenerative medicine and tissue engineering. This handbook focuses on all aspects of intelligent scaffolds, from basic science to industry to clinical applications. Its 10 parts, illustrated throughout with excellent figures, cover stem cell engineering research, drug delivery systems, nanomaterials and nanodevices, and novel and natural biomaterials. The book can be used by advanced undergraduate- and graduate-level students of stem cell and tissue engineering and researchers in macromolecular science, ceramics, metals for biomaterials, nanotechnology, chemistry, biology, and medicine, especially those interested in tissue engineering, stem cell engineering, and regenerative medicine.
Author : 吳連禎
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (11 download)
Download or read book Fabrication and Properties of Decellularized Porcine Annulus Fibrosus for Tissue Engineering in Spine Surgery written by 吳連禎 and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Neil D. Broom
Publisher : Cambridge University Press
ISBN 13 : 1108664768
Total Pages : 810 pages
Book Rating : 4.1/5 (86 download)
Download or read book The Soft–Hard Tissue Junction written by Neil D. Broom and published by Cambridge University Press. This book was released on 2018-11-29 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Richly illustrated throughout with actual tissue images, this innovative book shows that the soft-hard tissue junction is best understood in a biomechanical context. The authors describe their pioneering experimental methods, providing an essential structure-function framework for computational modelling, and thereby encouraging the development of more realistic, predictive models of this important tissue junction. Covering the three main musculoskeletal junctions of cartilage-bone, disc-vertebra, and ligament/tendon-bone, the relevant soft tissues are examined with respect to both their own inherent structure and their mode of integration with the hard tissue. The soft-hard tissue interface is explored with a focus on structural damage resulting from overloading, and its associated pathologies. Adopting a multiscale approach, ranging in structural resolution from the macro to fibril levels, this is a must-have guide to the field and an ideal resource for researchers seeking new and creative approaches for studying the joint and spine tissues.
