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Experiments With Fission Yeast
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Download or read book Fission Yeast written by Iain Hagan and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.
Book Synopsis Molecular Biology of the Fission Yeast by :
Download or read book Molecular Biology of the Fission Yeast written by and published by Elsevier. This book was released on 2012-12-02 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly researched yeast, which represents a system used by cell biologists, geneticists and molecular biologists, has been given only minimal coverage in the literature. Its properties make it an excellent organism for DNA and related biotechnology reseach. This book, which is the first attempt to collate existing information in one source, will be an invaluable aid to those initiating projects with this organism.
Book Synopsis The Molecular Biology of Schizosaccharomyces pombe by : Richard Egel
Download or read book The Molecular Biology of Schizosaccharomyces pombe written by Richard Egel and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.
Book Synopsis The Early Days of Yeast Genetics by : Michael N. Hall
Download or read book The Early Days of Yeast Genetics written by Michael N. Hall and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast genetics began with Winge's 1935 studies of S. cerevisiae in Copenhagen, and afterwards was pursued by Lindegren in the U.S. and Ephrussi in France. Genetic studies in S. pombe were pioneered by Leupold in the 1940s in Switzerland. Within four decades, not without controversies, both yeast species were recognized as essential models in eukaryotic molecular cell biology. In this remarkable volume, Hall and Linder have assembled the reminiscences of many early investigators whose pioneering studies in the years before 1975 brought yeast biology to its current maturity. These illustrated essays about the science, the events and the personalities involved capture a fascinating era, in the informal style made famous by Phage and the Origins of Molecular Biology. This is a book that all scientists interested in the development of modern genetics and molecular biology should have on their shelves.
Book Synopsis Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis by :
Download or read book Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis written by and published by Academic Press. This book was released on 2010-02-27 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. - Provides up-to-date methods necessary to study genes in yeast - Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations - Serves as an essential reference for any beginning or experienced researcher in the field
Book Synopsis The Evolution of Multicellularity by : Matthew D. Herron
Download or read book The Evolution of Multicellularity written by Matthew D. Herron and published by CRC Press. This book was released on 2022-06-07 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Among the most important innovations in the history of life is the transition from single-celled organisms to more complex, multicellular organisms. Multicellularity has evolved repeatedly across the tree of life, resulting in the evolution of new kinds of organisms that collectively constitute a significant portion of Earth’s biodiversity and have transformed the biosphere. This volume examines the origins and subsequent evolution of multicellularity, reviewing the types of multicellular groups that exist, their evolutionary relationships, the processes that led to their evolution, and the conceptual frameworks in which their evolution is understood. This important volume is intended to serve as a jumping-off point, stimulating further research by summarizing the topics that students and researchers of the evolution of multicellularity should be familiar with, and highlighting future research directions for the field.
Book Synopsis Yeast Genetics by : Jeffrey S. Smith
Download or read book Yeast Genetics written by Jeffrey S. Smith and published by Humana Press. This book was released on 2014-09-12 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
Book Synopsis The Evolution of Senescence in the Tree of Life by : Richard P. Shefferson
Download or read book The Evolution of Senescence in the Tree of Life written by Richard P. Shefferson and published by Cambridge University Press. This book was released on 2017-02-23 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: The existing theories on the evolution of senescence assume that senescence is inevitable in all organisms. However, recent studies have shown that this is not necessarily true. A better understanding of senescence and its underlying mechanisms could have far-reaching consequences for conservation and eco-evolutionary research. This book is the first to offer interdisciplinary perspectives on the evolution of senescence in many species, setting the stage for further developments. It brings together new insights from a wide range of scientific fields and cutting-edge research done on a multitude of different animals (including humans), plants and microbes, giving the reader a complete overview of recent developments and of the controversies currently surrounding the topic. Written by specialists from a variety of disciplines, this book is a valuable source of information for students and researchers interested in ageing and life history traits and populations.
Book Synopsis Handbook of Genetics by : Robert King
Download or read book Handbook of Genetics written by Robert King and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many modern geneticists attempt to elucidate the molecular basis of phenotype by utilizing a battery of techniques derived from physical chemistry on subcellular components isolated from various species of organisms. Volume 5 of the Handbook of Genetics provides explanations of the advantages and shortcomings of some of these revolutionary tech niques, and the nonspecialist is alerted to key research papers, reviews, and reference works. Much of the text deals with the structure and func tioning of the molecules bearing genetic information which reside in the nucleus and with the processing of this information by the ribosomes resid ing in the cytoplasm of eukaryotic cells. The mitochondria, which also live in the cytoplasm of the cells of all eukaryotes, now appear to be separate little creatures. These, as Lynn Margulis pointed out in Volume 1, are the colonial posterity of migrant prokaryotes, probably primitive bacteria that swam into the ancestral precursors of all eukaryotic cells and remained as symbionts. They have maintained themselves and their ways ever since, replicating their own DNA and transcribing an RNA quite different from that of their hosts. In a similar manner, the chloroplasts in all plants are self-replicating organelles presumably derived from the blue-green algae, with their own nucleic acids and ribosomes. Four chapters are devoted to the nucleic acids and the ribosomal components of both classes of these semi-independent lodgers. Finally, data from various sources on genetic variants of enzymes are tabulated for ready reference, and an evaluation of this information is attempted.
Book Synopsis Cell Biology by the Numbers by : Ron Milo
Download or read book Cell Biology by the Numbers written by Ron Milo and published by Garland Science. This book was released on 2015-12-07 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Book Synopsis Scientific Frontiers in Developmental Toxicology and Risk Assessment by : National Research Council
Download or read book Scientific Frontiers in Developmental Toxicology and Risk Assessment written by National Research Council and published by National Academies Press. This book was released on 2000-12-21 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
Book Synopsis Cell Cycle Checkpoint Control Protocols by : Howard B. Lieberman
Download or read book Cell Cycle Checkpoint Control Protocols written by Howard B. Lieberman and published by Humana Press. This book was released on 2003-11-14 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer.
Download or read book Enzymes of Epigenetics Part B written by and published by Elsevier. This book was released on 2016-07-14 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes of Epigenetics: Part B, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods that are employed in the study of epigenetic regulation, including structural, biochemical, molecular, biological, cellular, computational, and systems approaches. Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies, and small molecule epigenetic regulators and biological connections. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Contains two new volumes that cover research methods in enzymes of epigenetics - Covers such topics as chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Yeast Genetics by : David C. Amberg
Download or read book Methods in Yeast Genetics written by David C. Amberg and published by CSHL Press. This book was released on 2005 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Book Synopsis Inanimate Life by : George M. Briggs
Download or read book Inanimate Life written by George M. Briggs and published by . This book was released on 2021-07-16 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Biology of the Cell Cycle by : J. M. Mitchison
Download or read book The Biology of the Cell Cycle written by J. M. Mitchison and published by CUP Archive. This book was released on 1971-11-30 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
