Author : Jose Miguel Perez
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (124 download)
Book Synopsis Expanding the Frontiers of Plants Utilization by : Jose Miguel Perez
Download or read book Expanding the Frontiers of Plants Utilization written by Jose Miguel Perez and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biomass is the most abundant terrestrial source of renewable carbon that has the potential to replace fossil fuels to produce fuels and chemicals. For decades, the strategies for the utilization of its sugar-based fraction have involved the combination of physicochemical and biochemical techniques with the utilization of microorganisms, obtaining promising results. One of the major components of plant biomass is lignin, a phenolic heteropolymer that presents additional challenges because of its high recalcitrance and heterogeneity. As lignin valorization is predicted to be critical for the economic viability of a plant-based biorefinery, the development of appropriate tools is required. To make higher value products from lignin, recent studies have proposed the integration of chemical lignin deconstruction with biological upgrade by genetically engineered bacteria.In this work, we explored the potential of Novosphingobium aromaticivorans DSM12444 as a platform organism for lignin valorization in the context of a biomass-to-product pipeline that integrates chemical deconstruction with biological transformation. We used genetic tools to engineer the bacterium's metabolic mechanisms for production of 2-pyrone-4,6-dicarboxylic acid (PDC) and demonstrated its ability to utilize a broad range of phenolic compounds produced by chemical lignin depolymerization. The engineered strain was able to produce PDC with high yields using lignin depolymerized with different techniques and from multiple plant sources. Finally, we explored N. aromaticivorans enzymatic mechanisms for phenolic compound degradation and we were able to identify, using genomic, genetic and enzymology tools, enzymes responsible for critical reactions such as aromatic O-demethylation and aromatic ring opening. As a result, we created a 2nd generation engineered strain with improved product conversion yields with respect to the first engineered strain. Overall, this work provides valuable knowledge about the metabolic potential of bacteria for lignin valorization in the context of a biomass-to-product biorefinery and expands the portfolio of potentially useful platform microorganisms. Ongoing and future research on increasing the knowledge of N. aromaticivorans mechanisms for phenolic compound degradation, compatibility with different chemical biomass deconstruction techniques, expanding the portfolio of valuable products, and developing industrially relevant culture techniques will provide valuable information to fully understand N. aromaticivorans potential for lignin valorization.