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Author : Douglas Wayne Jackson
Publisher :
ISBN 13 :
Total Pages : 82 pages
Book Rating : 4.:/5 (198 download)
Download or read book Electrophoretic Studies of Human Alkaline Phosphatase written by Douglas Wayne Jackson and published by . This book was released on 1966 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Elizabeth Anne Stiene
Publisher :
ISBN 13 :
Total Pages : 110 pages
Book Rating : 4.:/5 (291 download)
Download or read book Electrophoretic Studies of Leukocyte Alkaline Phosphatase written by Elizabeth Anne Stiene and published by . This book was released on 1969 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Robert B. McComb
Publisher : Springer Science & Business Media
ISBN 13 : 1461329701
Total Pages : 998 pages
Book Rating : 4.4/5 (613 download)
Download or read book Alkaline Phosphatase written by Robert B. McComb and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 998 pages. Available in PDF, EPUB and Kindle. Book excerpt: There can be no doubt that alkaline phosphatase is one of the most extensively in vestigated of all enzymes. This has resulted from the ubiquity of its distribution, and from the ease and sensitivity with which its activity can be measured. Unfortunately, these wide-ranging but often superficial experimental studies have been followed up by intensive and systematic investigations in only a few limited areas of the biochemistry and chemical pathology of alkaline phosphatase. The result has been the accumulation of a scientific literature of intimidating proportions, and the inevitable rediscovery of already known facts about the enzyme. Scientists are taught early in their careers that, in the words of Sir John Herschel, "Hasty generalization is the bane of science. " Nevertheless, moments arrive in all spheres of scientific activity when generalization becomes essential, to codify and to select from the mass of data already accumulated, and to provide starting points for new developments and new lines of investigation. This is especially true in a field such as alkaline phosphatase research, in which very real dangers exist that the seeds of fundamental understanding will be lost amidst an unexamined harvest of empirical observations. The history of the study of alkaline phosphatase provides several instances when valuable generalizations have emerged. Occasionally, the conclusions drawn on the basis of available evidence were wrong; more frequently, they have stood the test of further experimentation, and always, they have provided new insights into the nature and proper ties of this enzyme.
Author : Joyce Evelyn Nutt
Publisher :
ISBN 13 :
Total Pages : 167 pages
Book Rating : 4.:/5 (184 download)
Download or read book Studies of Human Alkaline Phosphatases by Isoelectric Focusing and Electrophoresis written by Joyce Evelyn Nutt and published by . This book was released on 1972 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : David D. Weaver
Publisher :
ISBN 13 :
Total Pages : 114 pages
Book Rating : 4.:/5 (36 download)
Download or read book Histochemical, Fluorometric and Electrophoretic Analysis of Human Leukocytic Alkaline Phosphatase written by David D. Weaver and published by . This book was released on 1966 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : J. M. Jones
Publisher :
ISBN 13 :
Total Pages : 170 pages
Book Rating : 4.:/5 (16 download)
Download or read book Immunological and Electrophoretic Studies on Dog Alkaline Phosphatase, with Particular Reference to Serum written by J. M. Jones and published by . This book was released on 1972 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jean Marie Cons
Publisher :
ISBN 13 :
Total Pages : 126 pages
Book Rating : 4.:/5 (44 download)
Download or read book Electrophoretic Studies of Serum Proteins and Selected Enzymes in Non-pregnant, Pregnant, and Lactating Females and in Male Mice written by Jean Marie Cons and published by . This book was released on 1963 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : John George Konrath
Publisher :
ISBN 13 :
Total Pages : 114 pages
Book Rating : 4.:/5 (222 download)
Download or read book Electrophoretic Analysis of Glucose-6-phosphate Dehydrogenase, Alkaline Phosphatase, and Protein of Rabbit Serum, Erythrocytes, and Intraocular Fluid written by John George Konrath and published by . This book was released on 1974 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Angela Randall Whisnant
Publisher :
ISBN 13 :
Total Pages : 71 pages
Book Rating : 4.:/5 (561 download)
Download or read book Studies of Reversible Inhibition, Irreversible Inhibition and Activation of Alkaline Phosphatase by Capillary Electrophoresis written by Angela Randall Whisnant and published by . This book was released on 2004 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : M. Gouldie
Publisher :
ISBN 13 :
Total Pages : 67 pages
Book Rating : 4.:/5 (15 download)
Download or read book Analysis of the Molecular Forms of Placental Alkaline Phosphatase Using Capillary Electrophoresis and HPLC written by M. Gouldie and published by . This book was released on 1995 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Torgny Stigbrand
Publisher :
ISBN 13 :
Total Pages : 400 pages
Book Rating : 4.3/5 (91 download)
Download or read book Human Alkaline Phosphatases written by Torgny Stigbrand and published by . This book was released on 1984 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Connaught Medical Research Laboratories
Publisher :
ISBN 13 :
Total Pages : 82 pages
Book Rating : 4.:/5 (38 download)
Download or read book Starch-gel Electrophoresis written by Connaught Medical Research Laboratories and published by . This book was released on 1964 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : John Richard Beck
Publisher :
ISBN 13 :
Total Pages : 186 pages
Book Rating : 4.3/5 (129 download)
Download or read book Disc Electrophoresis of Turkey Serum Alkaline Phosphatase Isoenzymes Using Polyacrylamide Gel written by John Richard Beck and published by . This book was released on 1974 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies of Alkaline Phosphatase Inhibition by Metal Chelators Using Capillary Electrophoresis written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Alkaline phosphatase inhibition by metal chelators has been studied using capillary electrophoresis. The enzyme-inhibition assays were performed by electrophoretically mixing enzyme and inhibitor zones in a substrate filled capillary. Enzyme inhibition could be seen as a decrease in product formation as detected using laser-induced fluorescence. The enzyme-inhibition assays were adapted so they could be performed using a commercial CE system. Use of a commercial system is desirable for these assays due to ease of use and system features such as autosamplers and capillary and sampling cooling. This technique could prove useful for pharmaceutical industries as a screening tool for new drug therapies. Six metal chelators were studied as enzyme inhibitors using CE enzyme-inhibition assays. EDTA inhibition assays performed previously on a laboratory constructed CE system were compared to those using a commercial system. The assays performed on the commercial system showed irreversible inhibition at concentrations of 1.0 mM or less and activation at 2.0 mM. The opposite trend was seen with the laboratory constructed system. One hypothesis to explain this discrepancy is that two different water sources contained different concentrations of metal contaminants. The two water sources were used due to equipment failure making one source unavailable. The common metal chelators EGTA, NTA, and 1,10-phenanthroline all exhibited reversible inhibition of alkaline phophatase at concentrations of 0.08 to 2.0 mM. Crown ethers, which are not commonly used as enzyme inhibitors, were also studied. 12-crown-4 and 18-crown-6 both reversibly inhibited alkaline phophatase at concentrations of 0.08 to 0.3 mM. The reversible inhibition exhibited by the various chelators examined (excluding EDTA) could be due to a time dependent mechanism in which Zn2, which is required by ALP, is slowly removed, rendering the enzyme inactive. Alkaline phosphatase inhibition was also studied using on-column reagent addition. The enzyme was added on-column, through a gap reactor and mixed with the fluorescent enzyme substrate, Attophos, and zones of inhibitor. Theophylline, a reversible noncompetitive inhibitor, and sodium vanadate, a reversible competitive inhibitor were used. Enzyme inhibition could be easily seen as a decrease in product formation detected by laser-induced fluorescence.
Author : Leo P. Cawley
Publisher : Little Brown GBR
ISBN 13 :
Total Pages : 392 pages
Book Rating : 4.3/5 (91 download)
Download or read book Electrophoresis and Immunoelectrophoresis written by Leo P. Cawley and published by Little Brown GBR. This book was released on 1969 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Patrick J. Lincoln
Publisher : Springer Science & Business Media
ISBN 13 : 0896034437
Total Pages : 617 pages
Book Rating : 4.8/5 (96 download)
Download or read book Forensic DNA Profiling Protocols written by Patrick J. Lincoln and published by Springer Science & Business Media. This book was released on 1998-01-22 with total page 617 pages. Available in PDF, EPUB and Kindle. Book excerpt: This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.
Author : B. J. Haywood
Publisher : Butterworth-Heinemann
ISBN 13 :
Total Pages : 464 pages
Book Rating : 4.3/5 (91 download)
Download or read book Electrophoresis-technical Application written by B. J. Haywood and published by Butterworth-Heinemann. This book was released on 1969 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over 1600 abstracts presenting a selective survey of journals, monographs, and symposia describing electrophoresic techniques. Unnumbered citations are arranged by topics and chronologically in 2 sections, i.e., current literature (1965-1969) and background literature (pre-1965). No indexes.
