Dna Sequencing Protocols

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DNA Sequencing Protocols

Author : Annette M. Griffin
Publisher : Springer Science & Business Media
ISBN 13 : 1592595103
Total Pages : 386 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis DNA Sequencing Protocols by : Annette M. Griffin

Download or read book DNA Sequencing Protocols written by Annette M. Griffin and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of DNA Sequencing Protocols is to provide detailed practical procedures for the widest range of DNA sequencing meth ods, and we believe that all the vanguard techniques now being applied in this fast-evolving field are comprehensively covered. Sequencing technology has advanced at a phenomenal rate since the original methods were first described in the late 1970s and there is now a huge variety of strategies and methods that can be employed to determine the sequence of any DNA of interest. More recently, a large number of new and innovative sequencing techniques have been developed, including the use of such novel polymerases as Tag poly merase and Sequenase, the harnessing of PCR technology for linear amplification (cycle) sequencing, and the advent of automated DNA sequencers. DNA sequencing is surely one of the most important techniques in the molecular biology laboratory. Sequence analysis is providing an increasingly useful approach to the characterization of biological systems, and major multinational projects are already underway to map and sequence the entire genome of organisms, such as Escherichia coli, Saccharomyces cerevisiae, Caenorhabditis elegans, and Homo sapiens. Most scientists recognize the importance of DNA sequence data and perceive DNA sequencing as a valuable and indispensable aspect of their work. Recent technological advances, especially in the area of automated sequencing, have removed much of the drudg ery that was formerly associated with the technique, whereas innova tive computer software has greatly simplified the analysis and manipulation of sequence data.

DNA Sequencing Protocols

Author : Colin A Graham
Publisher : Humana Press
ISBN 13 : 9780896037168
Total Pages : 244 pages
Book Rating : 4.0/5 (371 download)

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Book Synopsis DNA Sequencing Protocols by : Colin A Graham

Download or read book DNA Sequencing Protocols written by Colin A Graham and published by Humana Press. This book was released on 2001-01-10 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

DNA Sequencing Protocols

Author : Colin A. Graham
Publisher :
ISBN 13 : 9781489942111
Total Pages : 256 pages
Book Rating : 4.9/5 (421 download)

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Book Synopsis DNA Sequencing Protocols by : Colin A. Graham

Download or read book DNA Sequencing Protocols written by Colin A. Graham and published by . This book was released on 2001 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.

DNA Sequencing II

Author : Jan Kieleczawa
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763733834
Total Pages : 400 pages
Book Rating : 4.7/5 (338 download)

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Book Synopsis DNA Sequencing II by : Jan Kieleczawa

Download or read book DNA Sequencing II written by Jan Kieleczawa and published by Jones & Bartlett Learning. This book was released on 2006 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Kieleczawa's second volume, DNA Sequencing II: Optimizing the Preparation and Clean-Up, is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA. This volume is divided into four comprehensive sections - DNA Purification, Cleanup of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies. The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory. This fascinating text is a wonderful addition to your molecular biology library.

DNA Sequencing

Author : Jan Kieleczawa
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763747824
Total Pages : 230 pages
Book Rating : 4.7/5 (478 download)

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Book Synopsis DNA Sequencing by : Jan Kieleczawa

Download or read book DNA Sequencing written by Jan Kieleczawa and published by Jones & Bartlett Learning. This book was released on 2005 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This in-depth new volume covers important topics in the field, including: biochemical and technological advances induced by Human Genome Project: proven and newly emerging methods of preparing DNA templates; effects of some widely used lab. reagents on DNA sequencing.

DNA Sequencing

Author : Anjana Munshi
Publisher : BoD – Books on Demand
ISBN 13 : 9535105647
Total Pages : 188 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis DNA Sequencing by : Anjana Munshi

Download or read book DNA Sequencing written by Anjana Munshi and published by BoD – Books on Demand. This book was released on 2012-04-20 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates methods of DNA sequencing and its application in plant, animal and medical sciences. It has two distinct sections. The one includes 2 chapters devoted to the DNA sequencing methods and the second includes 6 chapters focusing on various applications of this technology. The content of the articles presented in the book is guided by the knowledge and experience of the contributing authors. This book is intended to serve as an important resource and review to the researchers in the field of DNA sequencing.

DNA Sequencing Strategies

Author : Wilhelm Ansorge
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 220 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis DNA Sequencing Strategies by : Wilhelm Ansorge

Download or read book DNA Sequencing Strategies written by Wilhelm Ansorge and published by Wiley-Liss. This book was released on 1997 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This outstanding lab bench reference to the technology of DNA sequencing offers a collection of concise sequencing strategies and cloning protocols. Concentrates on the most up-to-the-minute automated methods and advanced approaches. Preparing DNA for sequencing, sequencing single- doubled-stranded DNA and their variations, how to optimise the primers used, preparation of DNA sequencing gels and the actual collection of results, labelling of DNA fragments for sequencing and data analysis are among the topics covered.

Current Protocols in Molecular Biology

Author : Frederick M. Ausubel
Publisher :
ISBN 13 :
Total Pages : 1020 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Current Protocols in Molecular Biology by : Frederick M. Ausubel

Download or read book Current Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 1987 with total page 1020 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Next Generation Sequencing

Author : Steven R. Head
Publisher : Humana Press
ISBN 13 : 9781493975129
Total Pages : 266 pages
Book Rating : 4.9/5 (751 download)

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Book Synopsis Next Generation Sequencing by : Steven R. Head

Download or read book Next Generation Sequencing written by Steven R. Head and published by Humana Press. This book was released on 2017-12-10 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications, method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field.

Methods in Molecular Biology: DNA sequencing protocols

Author : John M. Walker
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (84 download)

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Book Synopsis Methods in Molecular Biology: DNA sequencing protocols by : John M. Walker

Download or read book Methods in Molecular Biology: DNA sequencing protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic DNA and RNA Protocols

Author : Adrian J. Harwood
Publisher :
ISBN 13 :
Total Pages : 534 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Basic DNA and RNA Protocols by : Adrian J. Harwood

Download or read book Basic DNA and RNA Protocols written by Adrian J. Harwood and published by . This book was released on 1996-02-19 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Basic DNA and RNA Protocols experienced researchers and innovators present an essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. Its outstanding, thoroughly up-to-date, and eminently practical protocols make it an essential tool for today's molecular biologist.

DNA Barcodes

Author : Ida Lopez
Publisher : Humana Press
ISBN 13 : 9781617795909
Total Pages : 470 pages
Book Rating : 4.7/5 (959 download)

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Book Synopsis DNA Barcodes by : Ida Lopez

Download or read book DNA Barcodes written by Ida Lopez and published by Humana Press. This book was released on 2012-06-12 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.

PCR Protocols

Author : John M. S. Bartlett
Publisher : Springer Science & Business Media
ISBN 13 : 1592593844
Total Pages : 1083 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis PCR Protocols by : John M. S. Bartlett

Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Tag-based Next Generation Sequencing

Author : Matthias Harbers
Publisher : John Wiley & Sons
ISBN 13 : 352732819X
Total Pages : 609 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Tag-based Next Generation Sequencing by : Matthias Harbers

Download or read book Tag-based Next Generation Sequencing written by Matthias Harbers and published by John Wiley & Sons. This book was released on 2012-02-13 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be performed by tag-based approaches, where the focus shifts from 'sequencing power' to better means of data analysis and visualization for common users. Today Next Generation Sequence data require powerful bioinformatics expertise that has to be converted into easy-to-use data analysis tools. The book's intention is to give an overview on recently developed tag-based approaches along with means of their data analysis together with introductions to Next-Generation Sequencing Methods, protocols and user guides to be an entry for scientists to tag-based approaches for Next Generation Sequencing.

DNA Arrays

Author : Jang B. Rampal
Publisher : Springer Science & Business Media
ISBN 13 : 1592592341
Total Pages : 268 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis DNA Arrays by : Jang B. Rampal

Download or read book DNA Arrays written by Jang B. Rampal and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

Gene Mapping, Discovery, and Expression

Author : Minou Bina
Publisher : Springer Science & Business Media
ISBN 13 : 1597450979
Total Pages : 335 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Gene Mapping, Discovery, and Expression by : Minou Bina

Download or read book Gene Mapping, Discovery, and Expression written by Minou Bina and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completion of the sequence of the human genome represents an unpar- leled achievement in the history of biology. The project has produced nearly complete, highly accurate, and comprehensive sequences of genomes of s- eral organisms including human, mouse, drosophila, and yeast. Furthermore, the development of high-throughput technologies has led to an explosion of projects to sequence the genomes of additional organisms including rat, chimp, dog, bee, chicken, and the list is expanding. The nearly completed draft of genomic sequences from numerous species has opened a new era of research in biology and in biomedical sciences. In keeping with the interdisciplinary nature of the new scientific era, the chapters in Gene Mapping, Discovery, and Expression: Methods and Protocols recapitulate the necessity of integration of experimental and computational tools for solving - portant research problems. The general underlying theme of this volume is DNA sequence-based technologies. At one level, the book highlights the importance of databases, genome-browsers, and web-based tools for data access and ana- sis. More specifically, sequencing projects routinely deposit their data in p- licly available databases including GenBank, at the National Center of Biotechnology (NCBI) in the United States; EMBL, maintained by the European Bioinformatics Institute; and DDBJ, the DNA Data Bank of Japan. Currently, several browsers offer facile access to numerous genomic DNA sequences for gene mapping and data retrieval.

Ancient DNA

Author : Beth Alison Shapiro
Publisher :
ISBN 13 : 9781617795169
Total Pages : 247 pages
Book Rating : 4.7/5 (951 download)

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Book Synopsis Ancient DNA by : Beth Alison Shapiro

Download or read book Ancient DNA written by Beth Alison Shapiro and published by . This book was released on 2012-01-01 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ancient DNA presents an overview of the many of the protocols commonly used to study ancient DNA. These include laboratory instructions, extraction protocols, laboratory techniques, and suggestions for appropriate analytical approaches to make sense of the sequences obtained.