Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Dna Conjugates And Sensors
Download Dna Conjugates And Sensors full books in PDF, epub, and Kindle. Read online Dna Conjugates And Sensors ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis DNA Conjugates and Sensors by : Keith R Fox
Download or read book DNA Conjugates and Sensors written by Keith R Fox and published by Royal Society of Chemistry. This book was released on 2012-11-30 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applications of nucleic acids have developed recently to provide solutions for biosensors, diagnostic tools and as platforms for the assembly of complex structures. These developments have been possible as their base sequence can be used to assemble precise structures following simple and predictable rules. Self-assembled DNA can then be amplified using polymerase chain reaction (PCR) and this ultimately enables the preparation of synthetic nucleic acids. Their use as molecular tools or DNA-conjugates has recently been enhanced by the addition of other groups including enzymes, fluorophores and small molecules. Written by leaders in the field, this volume describes the preparation and application of these DNA-conjugates. Several have been used as sensors (aptamers, riboswitches and nanostructures) based on the ability of nucleic acids to adopt specific structures in the presence of ligands, whilst others link reporter groups such as proteins or fluorophores to RNA or DNA for detection, single molecule studies, and increasing the sensitivity of PCR. The book is relevant to researchers in areas related to analytical chemistry, chemical biology, medicinal chemistry, molecular pharmacology, and structural and molecular biology.
Book Synopsis Electrochemical DNA Biosensors by : Mehmet Sengun Ozsoz
Download or read book Electrochemical DNA Biosensors written by Mehmet Sengun Ozsoz and published by CRC Press. This book was released on 2012-04-23 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.
Book Synopsis Nanoparticle Sensors for the Detection of DNA Related to Disease by : Lee Barrett
Download or read book Nanoparticle Sensors for the Detection of DNA Related to Disease written by Lee Barrett and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this research was to develop stable dye-labelled oligonucleotidenanoparticle conjugates for the detection of DNA related to disease using surface enhanced resonance Raman scattering (SERRS) spectroscopy. In order to achieve this, both gold and silver nanoparticle conjugates were successfully functionalised with thiol- and thioctic acid-modified oligonucleotides. To investigate their viability as biosensors, the stability of the conjugates and their hybridisation efficacy was investigated. It was discovered that the thioctic acidmodified oligonucleotide-nanoparticle conjugates remain stable under these conditions for a substantial period of time compared to thiol-modified oligonucleotide nanoparticle conjugates. The nanoparticle conjugates were functionalised for use in surface enhanced resonance Raman scattering (SERRS) spectroscopy by the incorporation of an isothiocyanate dye molecule. Not only were the conjugates SERRS active, they were also proven to exhibit enhanced stability for both thiol- and thioctic acid-modified oligonucleotide nanoparticle conjugates. The thioctic acid-modified oligonucleotide nanoparticle conjugates were investigated for their use as sensors to detect specific DNA sequences. Both gold and silver nanoparticle conjugates were shown to retain their biological activity by hybridising with complementary DNA sequences. This was monitored colorimetrically, due to the characteristic colour changes associated with aggregation of gold and silver nanoparticles, and by monitoring the hybridisation-induced red-shift of the surface plasmon band by UV-Vis spectroscopy. The hybridisation-induced aggregation methodology was also extended to dye-labelled nanoparticle conjugates that were investigated by SERRS. A method for characterising oligonucleotide-nanoparticle conjugates using a fluorescent DNA intercalator, SYBR green I, was developed. This study showed that SYBR green I can be used as a rapid method for determining the melting profiles of individual nanoparticle conjugates and the hybridisation efficiency of the immobilized oligonucleotides.
Book Synopsis Biological DNA Sensor by : Ken Ishii
Download or read book Biological DNA Sensor written by Ken Ishii and published by Academic Press. This book was released on 2013-10-30 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological DNA Sensor defines the meaning of DNA sensing pathways and demonstrates the importance of the innate immune responses induced by double stranded DNA (dsDNA) through its influencing functions in disease pathology and immune activity of adjuvants for vaccines. Though discussed in specific subsections of existing books, dsDNA and its immunogenic properties has never received the complete treatment given in this book. Biological DNA Sensor approaches the impact of dsDNA's immunogenicity on disease and vaccinology holistically. It paints a complete and concise picture on the topic so you can understand this area of study and make more informed choices for your respective research needs. Chapters are authored by researchers who are renowned for their research focus, ensuring that this book provides the most complete views on the topics. - Multi-authored by a distinguished panel of world-class experts - Ideal source of information for those wanting to learn about DNA sensing - Provides in-depth explanations of DNA sensing pathways and the innate immune system, bridging the gap between them
Download or read book DNA Sensors and Inflammasomes written by and published by Academic Press. This book was released on 2019-08-25 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Sensors and Inflammasomes, Volume 625, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this release include Phosphorylation and dimerization of STING and IRF3, cGAS enzymology, Synthesis and identification of immuno-stimulatory CDNs, Tracking cGAS activity/ cGAMP formation using SPR/NMR, Using an enzyme coupled assay to track cGAS activity under steady states, Tracking the polymerization of DNA sensors, inflammasome receptors, and downstream signaling partners using FRET, NLRC4 structure, Tracking TREX1 activity, DNA association and dissociation kinetics of PARP1, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on DNA sensors and inflammasomes
Book Synopsis Electrochemical Biosensors by : Serge Cosnier
Download or read book Electrochemical Biosensors written by Serge Cosnier and published by CRC Press. This book was released on 2015-01-26 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy
Book Synopsis Miniaturized Optical DNA Sensors Based on Conjugated Polymers by : Anupama Rao Gular Srinivas
Download or read book Miniaturized Optical DNA Sensors Based on Conjugated Polymers written by Anupama Rao Gular Srinivas and published by . This book was released on 2013 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of highly sensitive and selective DNA sensors has fuelled applications in a wide range of fields including medical diagnostics, forensics, biodefense, food contamination and environment monitoring. Recently, conjugated polymers (CPs) have drawn attention as attractive novel materials for biosensors due to their tuneable optical and electronic properties and ability to respond to minor changes in their environment. The aim of this research was to develop miniaturised optical DNA sensors based on photoluminescent CPs. Investigations towards the effect of systematically substituting carboxylic acid functionalized CPs with polar methoxy, mono-ethylene glycol, di-ethylene glycol and tri-ethylene glycol sidechains on the solubility, optical and electrochemical properties were conducted. The resultant CPs were characterised using a range of techniques such as nuclear magnetic resonance (NMR), fourier infrared spectroscopy (FTIR), ultra-violet (UV-Vis) visible spectroscopy and fluorescence spectroscopy. Furthermore, three different novel optical DNA sensors on magnetic beads were designed and developed using the synthesized CPs. DNA hybridisation was monitored using fluorescence resonance energy transfer (FRET) and superquenching readout methodologies. These sensors exhibit desirable characteristics such as ease of handling (through magnetic deployment). All the three different sensing platforms were able to discriminate mismatches in the target sequences, theoretical detection limits to target DNA concentrations were calculated between 0.01 pM and 18.8 pM, improving resistance to non-specific interactions with DNA and proteins, fulfilling the requirements for a selective, sensitive, mobile, cost effective miniaturised DNA sensor.
Book Synopsis The Development and Application of a Sequence-selective DNA Sensor by : Kelly M. Millan
Download or read book The Development and Application of a Sequence-selective DNA Sensor written by Kelly M. Millan and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis DNA in Supramolecular Chemistry and Nanotechnology by : Eugen Stulz
Download or read book DNA in Supramolecular Chemistry and Nanotechnology written by Eugen Stulz and published by John Wiley & Sons. This book was released on 2015-09-28 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.
Download or read book Paper Based Sensors written by and published by Elsevier. This book was released on 2020-06-25 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: Paper Based Sensors, Volume 89, the latest release in this comprehensive series that gathers the most important issues relating to the design and application of these cost-effective devices used in many industries, including health and environment diagnostics, safety and security, chemistry, optics, electrochemistry, nanoscience and nanotechnologies, presents the latest updates in the field. Chapters in this new release include Exploring paper as a substrate for electrochemical micro-devices, Paper-based sensors for application in biological compound detection, Printed paper-based (bio)sensors: design, fabrication and applications, Paper-based electrochemical sensing devices, Multifarious aspects of electrochemical paper-based (bio)sensors, Paper Based Biosensors for Clinical and Biomedical Applications, and more. Provides updates on the latest design in paper-based sensors using various nano and micromaterials Includes optical/electrical-based detection modes integrated within paper-based platforms Covers applications of paper-based platforms in diagnostics and other industries
Book Synopsis Microfabricated Sensors by : Richard Joseph Kordal
Download or read book Microfabricated Sensors written by Richard Joseph Kordal and published by . This book was released on 2002 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes the important application of the Bio- or DNA-Chips in biomedical research such as gene expression studies and methods of construction. Details novel optical sensing and processing technologies that are being employed by researchers in this field. Specific topics include DNA Chips, detection technologies, and optical sensing and processing.
Book Synopsis Biological Sensing and DNA Templated Electronics Using Conjugated Polymers by : Per Björk
Download or read book Biological Sensing and DNA Templated Electronics Using Conjugated Polymers written by Per Björk and published by . This book was released on 2007 with total page 65 pages. Available in PDF, EPUB and Kindle. Book excerpt: Likt en termometer som kan mäta en förändring i den omgivande temperaturen kan en sensor inom den biologiska världen mäta exempelvis en förändring hos en viss biomolekyl eller om den interagerar med en annan biomolekyl. I första delen av arbetet som beskrivs i denna avhandling har en speciell typ av polymer använts för att detektera biomolekylers interaktion. Dessa kallas konjugerade polymerer och har en upprepad struktur i sin kemiska formel som är alternerade dubbel- och enkelbindningar mellan kolatomer.
Book Synopsis Synchrotron Radiation in Materials Science by : Chunhai Fan
Download or read book Synchrotron Radiation in Materials Science written by Chunhai Fan and published by John Wiley & Sons. This book was released on 2018-05-29 with total page 846 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the long-felt need for in-depth information on one of the most advanced material characterization methods, a top team of editors and authors from highly prestigious facilities and institutions covers a range of synchrotron techniques that have proven useful for materials research. Following an introduction to synchrotron radiation and its sources, the second part goes on to describe the various techniques that benefit from this especially bright light, including X-ray absorption, diffraction, scattering, imaging, and lithography. The thrid and final part provides an overview of the applications of synchrotron radiation in materials science. bridging the gap between specialists in synchrotron research and material scientists, this is a unique and indispensable resource for academic and industrial researchers alike.
Download or read book DNA Nanotechnology written by Chunhai Fan and published by Springer Science & Business Media. This book was released on 2013-03-12 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts. Part I, Elements of DNA Nanotechnology, provides extensive basic information on DNA nanotechnology. Part II, Static and Dynamic DNA Nanotechnology, describes the design and fabrication of static and dynamic DNA nanostructures. Recent advances in DNA origami, DNA walkers and DNA nanodevices are all covered in this part. Part III, Applications of DNA Nanotechnology, introduces a variety of applications of DNA nanotechnology, including biosensing, computation, drug delivery, etc. Together these provide a comprehensive overview of this emerging area and its broad impact on biological and medical sciences. This book is intended for post-graduates, post-doctoral researchers and research scientists who are interested in expanding their knowledge of DNA nanotechnology. It provides readers an impression of the latest developments in this exciting filed.
Book Synopsis Development of Functional DNA-based Sensors and Investigations Into Their Mechanism by : Nandini Nagraj
Download or read book Development of Functional DNA-based Sensors and Investigations Into Their Mechanism written by Nandini Nagraj and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery that nucleic acids could perform functional roles in addition to being genetic materials carriers opened doors to a new paradigm in nucleic acid chemistry. Catalytic DNA molecules also known as deoxyribozymes or DNAzymes were first isolated in 1994 through an in vitro selection procedure and have since been engineered and isolated to perform various functions that include both RNA and DNA cleavage and ligation. The 8-17 DNAzyme is an RNA-cleaving DNAzyme that has shown high selectivity for Pb2+ under different selection conditions. It has been explored extensively in terms of its applications for bio-sensing as well as for exploring its mechanism from a more fundamental perspective. A critical barrier of DNA-based sensors for practical applications, such as environmental monitoring, is their highly variable sensing performance with changing temperatures, due to the reliance of sensor design on temperature-dependent hybridization. In Chapter 2, this issue has been addressed through the introduction of mismatches in the DNA hybridization arms of this Pb2+-specific 8-17 DNAzyme and these fluorescent sensors can resist temperature-dependent variations from 4 °C to 30 °C. The strategy of using mismatches to tune the temperature dependence is a novel and inexpensive method that can be applied in other nucleic acid sensors for either metal ions or other molecular targets. Currently there is no structure, (either X-ray or NMR) available for the 8-17 DNAzyme. Hence, understanding its mechanism has posed a challenge, particularly in regard to the high selectivity of Pb2+ for this DNAzyme. In Chapter 3, the systematic activity, folding and structural studies of the 8-17 DNAzyme with both monovalent and divalent metal ions has been carried out. The results obtained suggest a clear trend between the folding and activity of all the metal ions studied, the lower the activity, the lesser the folding and vice-versa. Structural studies based on CD and folding studies based on FRET have demonstrated that Pb2+ behaves in a manner that is different from other metal ions and hence it is hypothesized that the 8-17 DNAzyme may have a specific binding pocket for Pb2+. The possibility that the 8-17 DNAzyme might have a metal ion binding pocket for Pb2+ has been investigated in Chapter 4, through systematic phosphorothioate (PS) modifications on the backbone of the DNAzyme. Kinetic assays with the PS modified bases have shown that there are specific bases on the enzyme strand which are important for activity mainly in the presence of Pb2+. The activities of the identical PS modified enzymes are, however, not significantly altered in the presence of Mg2+ and Cd2+. 31P NMR has been used as an additional tool to directly visualize the backbone phosphates since a single PS modification shifts the signal of the phosphate downfield by ~50 ppm. These results, in conjunction, have led to the identification of a proposed metal ion binding site, specifically a potential Pb2+-binding site for the 8-17 DNAzyme. The starting point towards the development of a successful functional nucleic acid-based sensor is its isolation and this is done through in vitro selection. In vitro selection to isolate DNAzymes for Hg2+ and Cd2+ and the use of negative selections to overcome Pb2+ interference at various stages of selection has been described in Chapter 5. Chapter 6 describes the structure-switching strategy to isolate DNA aptamers specific for endotoxins. It is anticipated that the results obtained from the current study and future characterizations will lead to the development of functional DNA sensors for endotoxins.
Book Synopsis Nucleic Acids Conjugates for Biotechnological Applications by : Tamaki Endoh
Download or read book Nucleic Acids Conjugates for Biotechnological Applications written by Tamaki Endoh and published by Mdpi AG. This book was released on 2021-12-06 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: Life in biological systems is maintained by the cooperative actions of various biomolecules. With the development of chemical and biological technologies related to nucleic acids, the details of the mechanisms of such cooperative actions between nucleic acids and other biomolecules have been elucidated and further applied in various applications. In the papers published in this Special Issue, advanced research works involved in nucleic acid conjugates are reported in wide application fields, such as artificial gene regulation, biomolecular sensing, and therapeutics from leading scientists in nucleic acids chemistry and engineering.
Book Synopsis Modified Nucleic Acids by : Kazuhiko Nakatani
Download or read book Modified Nucleic Acids written by Kazuhiko Nakatani and published by Springer. This book was released on 2016-04-04 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.
